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J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Visceral white-nodules disease (VWND), caused by Pseudomonas plecoglossicida, poses a severe threat to the large yellow croaker (Larimichthys crocea) aquaculture industry. Although breeding resistant strains is a promising strategy, the molecular basis of disease resistance in this host remains poorly understood. Here, 1500 fish were artificially infected, and extreme phenotypes (30 resistant, RL; 30 susceptible, SL) were selected based on survival time and liver pathogen load. Liver histopathology revealed that RL fish maintained intact architecture with only mild vacuolation, whereas SL fish exhibited widespread necrosis, inflammation, and hemosiderin deposition. Consistently, RL fish showed lower MDA levels and higher GSH-Px activity and TAC. Transcriptomic analysis identified 172 differentially expressed genes (DEGs): RL fish were characterized by upregulation of anti-inflammatory and tissue-protective genes (Epo, CAV3) and downregulation of pro-coagulant factors (PAI1, K1kb1). Proteomic analysis identified 111 differentially expressed proteins, with significantly enriched pathways including the peroxisome, pentose phosphate, and phagosome pathways. Integrated cross-omics analysis revealed eight co-enriched KEGG pathways; among them, arginine\/proline metabolism, phagosome, oxidative phosphorylation, and focal adhesion were consistently upregulated in the RL group. These findings suggest that effective resistance to VWND in L. crocea may involve a coordinated, multi-layered defense program encompassing redox balance, regulated immune responses, metabolic reprogramming, and cellular homeostasis. Cross-omics-supported candidate factors (e.g., P4ha1, COX6B, RAB5A, CAV3) represent promising targets for functional validation via DNA-level experiments in independent sample sets, and the prominent enrichment of arginine-proline metabolism indicates a potential target for dietary intervention that merits further investigation.<\/jats:p>","DOI":"10.3390\/ijms27167208","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T12:53:48Z","timestamp":1786539228000},"page":"7208","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Liver Transcriptomic and Proteomic Analysis Reveals Resistance Mechanisms Against Pseudomonas plecoglossicida in Larimichthys crocea"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-3389-7178","authenticated-orcid":false,"given":"Ting","family":"Ye","sequence":"first","affiliation":[{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"},{"name":"Zhejiang Key Laboratory of Coastal Biological Germplasm Resources Conservation and Utilization, Wenzhou 325005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajie","family":"Zhu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"},{"name":"Zhejiang Key Laboratory of Coastal Biological Germplasm Resources Conservation and Utilization, Wenzhou 325005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Liang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"},{"name":"Zhejiang Key Laboratory of Coastal Biological Germplasm Resources Conservation and Utilization, Wenzhou 325005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dandan","family":"Guo","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"},{"name":"Zhejiang Key Laboratory of Coastal Biological Germplasm Resources Conservation and Utilization, Wenzhou 325005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yilian","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"},{"name":"Zhejiang Key Laboratory of Coastal Biological Germplasm Resources Conservation and Utilization, Wenzhou 325005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bao","family":"Lou","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"},{"name":"Zhejiang Key Laboratory of Coastal Biological Germplasm Resources Conservation and Utilization, Wenzhou 325005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3949-9067","authenticated-orcid":false,"given":"Feng","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China"},{"name":"Zhejiang Key Laboratory of Coastal Biological Germplasm Resources Conservation and Utilization, Wenzhou 325005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.repbre.2025.03.006","article-title":"Germplasm innovation of large yellow croaker and its research progress","volume":"5","author":"Liu","year":"2025","journal-title":"Reprod. Breed."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1002\/aah.10215","article-title":"The bacterial pathogen Pseudomonas plecoglossicida, its epidemiology, virulence factors, vaccine development, and host-pathogen interactions","volume":"36","author":"Mao","year":"2024","journal-title":"J. Aquat. Anim. Health"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.1111\/jfd.13206","article-title":"An outbreak of visceral white nodules disease caused by Pseudomonas plecoglossicida at a water temperature of 12 \u00b0C in cultured large yellow croaker (Larimichthys crocea) in China","volume":"43","author":"Li","year":"2020","journal-title":"J. Fish Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1099\/00207713-50-1-83","article-title":"Pseudomonas plecoglossicida sp. nov., the causative agent of bacterial haemorrhagic ascites of ayu, Plecoglossus altivelis","volume":"50","author":"Nishimori","year":"2000","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Huang, L.X., Liu, W.J., Jiang, Q.L., Zuo, Y.F., Su, Y.Q., Zhao, L.M., Qin, Y.X., and Yan, Q.P. (2018). Integration of transcriptomic and proteomic approaches reveals the temperature-dependent virulence of Pseudomonas plecoglossicida. Front. Cell Infect. Microbiol., 8.","DOI":"10.3389\/fcimb.2018.00207"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"111147","DOI":"10.1016\/j.fsi.2026.111147","article-title":"Mechanism of Pseudomonas plecoglossicida T6SS effector vgrG1a in disrupting hepatic immunometabolic homeostasis in large yellow croaker","volume":"170","author":"Peng","year":"2026","journal-title":"Fish Shellfish Immunol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1038\/cmi.2016.3","article-title":"Liver immunology and its role in inflammation and homeostasis","volume":"13","author":"Robinson","year":"2016","journal-title":"Cell Mol. Immunol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1958","DOI":"10.1111\/jfb.14901","article-title":"Immune defence mechanisms presented in liver homogenates and bile of gilthead seabream (Sparus aurata)","volume":"99","author":"Cuesta","year":"2021","journal-title":"J. Fish Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"109467","DOI":"10.1016\/j.fsi.2024.109467","article-title":"Molecular characterization, antibacterial and immunoregulatory activities of liver-expressed antimicrobial peptide 2 in black rockfish, Sebastes schlegelii","volume":"147","author":"Zhang","year":"2024","journal-title":"Fish Shellfish Immunol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"vkaf319","DOI":"10.1093\/jimmun\/vkaf319","article-title":"Negative regulation of a new collectin-11\/calreticulin-mediated NF-\u03baB pathway by miR-194b-3p in antibacterial immunity of Takifugu obscurus","volume":"215","author":"Huang","year":"2026","journal-title":"J. Immunol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"110533","DOI":"10.1016\/j.fsi.2025.110533","article-title":"BSA-seq and transcriptome analysis identification candidate genes associated with Pseudomonas plecoglossicida resistance in the large yellow croaker (Larimichthys crocea)","volume":"165","author":"Ye","year":"2025","journal-title":"Fish Shellfish Immunol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1016\/j.fsi.2011.07.024","article-title":"Dietary arginine and repeated handling increase disease resistance and modulate innate immune mechanisms of Senegalese sole (Solea senegalensis Kaup, 1858)","volume":"31","author":"Costas","year":"2011","journal-title":"Fish Shellfish Immunol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1007\/s11427-013-4557-2","article-title":"Transcriptomics: Advances and approaches","volume":"56","author":"Dong","year":"2013","journal-title":"Sci. China Life Sci."},{"key":"ref_14","first-page":"100886","article-title":"Transcriptome analysis revealed multiple immune processes and energy metabolism pathways involved in the defense response of the large yellow croaker Larimichthys crocea against Pseudomonas plecoglossicida","volume":"40","author":"Zhang","year":"2021","journal-title":"Comp. Biochem. Physiol. D Genom. Proteom."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"110526","DOI":"10.1016\/j.fsi.2025.110526","article-title":"Transcriptomic analysis reveals the role of MAPK signaling pathway in IgM+ B cells against Pseudomonas plecoglossicida infection in large yellow croaker (Larimichthys crocea)","volume":"165","author":"Zhu","year":"2025","journal-title":"Fish Shellfish Immunol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1016\/j.fsi.2022.07.036","article-title":"Skin immune response to Aeromonas hydrophila infection in crucian carp Carassius auratus revealed by multi-omics analysis","volume":"127","author":"Li","year":"2022","journal-title":"Fish Shellfish Immunol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"108704","DOI":"10.1016\/j.fsi.2023.108704","article-title":"Integration of transcriptome and proteome analyses reveals the regulation mechanisms of Larimichthys polyactis liver exposed to heat stress","volume":"135","author":"Liu","year":"2023","journal-title":"Fish Shellfish Immunol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1038\/nrg3185","article-title":"Insights into the regulation of protein abundance from proteomic and transcriptomic analyses","volume":"13","author":"Vogel","year":"2012","journal-title":"Nat. Rev. Genet."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1111\/jnc.14664","article-title":"Poor transcript-protein correlation in the brain: Negatively correlating gene products reveal neuronal polarity as a potential cause","volume":"149","author":"Moritz","year":"2019","journal-title":"J. Neurochem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1002\/alr.22315","article-title":"Translating transcription: Proteomics in chronic rhinosinusitis with nasal polyps reveals significant discordance with messenger RNA expression","volume":"9","author":"Workman","year":"2019","journal-title":"Int. Forum. Allergy Rhinol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"109243","DOI":"10.1016\/j.fsi.2023.109243","article-title":"An aluminium adjuvant compound with ginseng stem leaf saponins enhances the potency of inactivated Pseudomonas plecoglossicida vaccine in large yellow croaker (Larimichthys crocea)","volume":"144","author":"Sun","year":"2024","journal-title":"Fish Shellfish Immunol."},{"key":"ref_22","first-page":"100864","article-title":"Combining of transcriptomic and proteomic data to mine immune-related genes and proteins in the liver of Cynoglossus semilaevis challenged with Vibrio anguillarum","volume":"39","author":"Qi","year":"2021","journal-title":"Comp. Biochem. Physiol. D Genom. Proteom."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"743931","DOI":"10.1016\/j.aquaculture.2026.743931","article-title":"Progression of liver injury and apoptosis in small yellow croaker (Larimichthys polyactis) during Pseudomonas plecoglossicida infection","volume":"620","author":"Zhu","year":"2026","journal-title":"Aquaculture"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"739045","DOI":"10.1016\/j.aquaculture.2022.739045","article-title":"Disentangling genetic variation for endurance and resistance to visceral white-nodules disease in large yellow croaker (Larimichthys crocea) using genome information","volume":"564","author":"Li","year":"2023","journal-title":"Aquaculture"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.1007\/s10499-019-00376-4","article-title":"A genome-wide association study of resistance to Pseudomonas plecoglossicida infection in the large yellow croaker (Larimichthys crocea)","volume":"27","author":"Wan","year":"2019","journal-title":"Aquacult. Int."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"9828","DOI":"10.1073\/pnas.88.21.9828","article-title":"Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations","volume":"88","author":"Michelmore","year":"1991","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1016\/j.molp.2018.12.018","article-title":"QTG-Seq accelerates QTL fine mapping through QTL partitioning and whole-genome sequencing of bulked segregant samples","volume":"12","author":"Zhang","year":"2018","journal-title":"Mol. Plant."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"111252","DOI":"10.1016\/j.fsi.2026.111252","article-title":"Serial comparative transcriptomics reveals dynamic and coordinated immune-metabolic responses in the liver-spleen axis of Oplegnathus punctatus to Edwardsiella piscicida infection","volume":"173","author":"Yang","year":"2026","journal-title":"Fish Shellfish Immunol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1007\/s10126-014-9569-9","article-title":"A genome scan for quantitative trait loci associated with Vibrio anguillarum infection resistance in Japanese flounder (Paralichthys olivaceus) by bulked segregant analysis","volume":"16","author":"Wang","year":"2014","journal-title":"Mar. Biotechnol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.fsi.2019.02.041","article-title":"Whole-genome resequencing from bulked-segregant analysis reveals gene set based association analyses for the Vibrio anguillarum resistance of turbot (Scophthalmus maximus)","volume":"88","author":"Zhang","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Menon, S.V., Kumar, A., Middha, S.K., Paital, B., Mathur, S., Johnson, R., Kademan, A., Usha, T., Hemavathi, K.N., and Dayal, S. (2023). Water physicochemical factors and oxidative stress physiology in fish, a review. Front. Environ. Sci., 11.","DOI":"10.3389\/fenvs.2023.1240813"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1007\/s10499-024-01741-8","article-title":"Dietary intervention of propolis and\/or turmeric boosted growth, hematology, biochemical profile, and antioxidant-immune responses and their associated gene expression in Nile tilapia (Oreochromis niloticus) challenged with Edwardsiella tarda","volume":"33","author":"Edrees","year":"2025","journal-title":"Aquacult. Int."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.fsi.2020.02.010","article-title":"Orange-spotted grouper (Epinephelus coioides) NADPH oxidase: Cloning and expression analysis after Cryptocaryon irritans infection","volume":"99","author":"Wang","year":"2020","journal-title":"Fish Shellfish Immunol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"145","DOI":"10.3329\/jsr.v12i1.41716","article-title":"Oxidative stress in fish: A review","volume":"12","author":"Chowdhury","year":"2020","journal-title":"J. Sci. Res."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Zou, J., and Secombes, C.J. (2016). The function of fish cytokines. Biology, 5.","DOI":"10.3390\/biology5020023"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Parida, S., and Sahoo, P.K. (2024). Antioxidant defence in Labeo rohita to biotic and abiotic stress: Insight from mRNA expression, molecular characterization and recombinant protein-based ELISA of catalase, glutathione peroxidase, CuZn superoxide dismutase, and glutathione S-transferase. Antioxidants, 13.","DOI":"10.3390\/antiox13010018"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"146589","DOI":"10.1242\/dev.146589","article-title":"Wnt signaling in development and tissue homeostasis","volume":"145","author":"Steinhart","year":"2018","journal-title":"Development"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1084\/jem.20151570","article-title":"Immunometabolism governs dendritic cell and macrophage function","volume":"213","author":"Pearce","year":"2016","journal-title":"J. Exp. Med."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3890","DOI":"10.1016\/j.molcel.2025.09.028","article-title":"Cell-death networks","volume":"85","author":"Kashkar","year":"2025","journal-title":"Mol. Cell"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.micinf.2011.10.005","article-title":"The pleiotropic effects of erythropoietin in infection and inflammation","volume":"14","author":"Nairz","year":"2012","journal-title":"Microbes Infect."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Zhang, X., He, D., Jia, J., Liang, F., Mei, J., Li, W., Liu, T.T., Wang, Z.Y., Liu, Y., and Zhang, F.X. (2022). Erythropoietin mediates re-programming of endotoxin-tolerant macrophages through PI3K\/AKT signaling and protects mice against secondary infection. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.938944"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"S\u00e1nchez-Roncancio, C., Garc\u00eda, B., Gallardo-Hidalgo, J., and Y\u00e1\u00f1ez, J.M. (2022). GWAS on imputed whole-genome sequence variants reveal genes associated with resistance to Piscirickettsia salmonis in rainbow trout (Oncorhynchus mykiss). Genes, 14.","DOI":"10.3390\/genes14010114"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2020","DOI":"10.1111\/j.1538-7836.2011.04473.x","article-title":"Plasminogen activator inhibitor type I contributes to protective immunity during experimental Gram-negative sepsis (melioidosis)","volume":"9","author":"Kager","year":"2011","journal-title":"J. Thromb. Haemost."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Oehmcke-Hecht, S., and K\u00f6hler, J. (2018). Interaction of the human contact system with pathogens-an update. Front. Immunol., 9.","DOI":"10.3389\/fimmu.2018.00312"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"3027","DOI":"10.1128\/JVI.00552-12","article-title":"Mandarin fish caveolin 1 interaction with major capsid protein of infectious spleen and kidney necrosis virus and its role in early stages of infection","volume":"87","author":"Jia","year":"2013","journal-title":"J. Virol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"936","DOI":"10.1126\/science.1214935","article-title":"Disease tolerance as a defense strategy","volume":"335","author":"Medzhitov","year":"2012","journal-title":"Science"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1755","DOI":"10.1016\/j.bbamcr.2006.09.006","article-title":"Peroxisomes and oxidative stress","volume":"1763","author":"Schrader","year":"2006","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1111\/brv.12140","article-title":"The return of metabolism: Biochemistry and physiology of the pentose phosphate pathway","volume":"90","author":"Stincone","year":"2015","journal-title":"Biol. Rev. Camb. Philos. Soc."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1189\/jlb.68.5.627","article-title":"Dynamics of rab5 activation in endocytosis and phagocytosis","volume":"68","author":"Roberts","year":"2000","journal-title":"J. Leukoc. Biol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.ceb.2008.03.015","article-title":"The V-type H+-ATPase in vesicular trafficking","volume":"20","author":"Marshansky","year":"2008","journal-title":"Curr. Opin. Cell Biol."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Rath, M., M\u00fcller, I., Kropf, P., Closs, E.I., and Munder, M. (2014). Metabolism via arginase or nitric oxide synthase: Two competing arginine pathways in macrophages. Front. Immunol., 5.","DOI":"10.3389\/fimmu.2014.00532"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.fsi.2015.02.027","article-title":"Effect of dietary arginine on the immune response and gene expression in head kidney and spleen following infection of Jian carp with Aeromonas hydrophila","volume":"44","author":"Chen","year":"2015","journal-title":"Fish Shellfish Immunol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1146\/annurev-immunol-032713-120236","article-title":"Metabolic regulation of immune responses","volume":"32","author":"Ganeshan","year":"2014","journal-title":"Annu. Rev. Immunol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"aaf2154","DOI":"10.1126\/science.aaf2154","article-title":"Programmed necrosis in inflammation: Toward identification of the effector molecules","volume":"352","author":"Wallach","year":"2016","journal-title":"Science"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1016\/j.cell.2017.02.004","article-title":"mTOR signaling in growth, metabolism, and disease","volume":"168","author":"Saxton","year":"2017","journal-title":"Cell"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"738421","DOI":"10.1016\/j.aquaculture.2022.738421","article-title":"Genomic selection for visceral white-nodules diseases resistance in large yellow croaker","volume":"559","author":"Bai","year":"2022","journal-title":"Aquaculture"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Chapagain, P., Ali, A., and Salem, M. (2023). Dual RNA-Seq of flavobacterium psychrophilum and its outer membrane vesicles distinguishes genes associated with susceptibility to bacterial cold-water disease in rainbow trout (Oncorhynchus mykiss). Pathogens, 12.","DOI":"10.3390\/pathogens12030436"},{"key":"ref_58","first-page":"328","article-title":"Establishment and application of a quantitative method for detecting of Pseudomonas Plecoglossicid infection in large yellow croaker (Larimichthys crocea)","volume":"24","author":"Cui","year":"2019","journal-title":"J. Jimei Univ."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/16\/7208\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T12:55:49Z","timestamp":1786539349000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/16\/7208"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,12]]},"references-count":58,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["ijms27167208"],"URL":"https:\/\/doi.org\/10.3390\/ijms27167208","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,12]]}}}