{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T09:36:13Z","timestamp":1786700173692,"version":"build-2736575974"},"reference-count":95,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>This study explores the biotechnological applications of the ovine secretome by mapping protein expression trends and lipidomic dynamics in Chios (n = 5) and Karagouniko (n = 10) ewes during early lactation (1, 3, 5, 9, and 15 days post-partum-DPP). Immunoglobulin-G (IgG) expression was monitored via ELISA, lactoferrin (Lf) profiles were quantified using densitometric Tricine-SDS-PAGE, and fatty acid (FA) patterns were resolved by gas chromatography. Two-way mixed-factor repeated-measures ANOVA and Principal Component Analysis (PCA) revealed a highly significant temporal transition (p &lt; 0.0001) across all components. Total IgG expression dropped sharply from DPP 1 (62.0\u201383.5 mg\/mL) to day 15 (12.8\u201321.4 mg\/mL), displaying a significant breed-by-time interaction (p &lt; 0.0001), while the resilient Karagouniko breed exhibited a more buffered clearance slope. Conversely, local Lf mucosal expression followed a parallel, breed-independent temporal decay trajectory (p = 0.5877). Concurrently, saturated FAs decreased while monounsaturated and polyunsaturated fractions rose (p &lt; 0.01), optimizing health-related lipid indices over time, including Atherogenicity (IA), Thrombogenicity (IT), and Health-Promoting (HPI). Unsupervised PCA successfully isolated early colostrum profiles based on coordinated immunological and lipidomic vectors. These findings demonstrate that tracking early secretome trajectories serves as a valuable, non-invasive indicator for translational veterinary medicine, establishing these genotype-specific fractions as potential substrates for future evaluation in bioactive functional food matrices research.<\/jats:p>","DOI":"10.3390\/ijms27167252","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T09:04:01Z","timestamp":1786698241000},"page":"7252","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biotechnological Advancements in Ovine Bioactives: Mapping Protein Expression Trends and Immuno-Lipidomic Dynamics for Functional Food Applications"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5516-5126","authenticated-orcid":false,"given":"Kostas A.","family":"Triantaphyllopoulos","sequence":"first","affiliation":[{"name":"Department of Biotechnology, Laboratory of Genetics, School of Applied Biology and Biotechnology, Agricultural University of Athens, 75 Iera Odos Street, 11855 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyriakos","family":"Bastas","sequence":"additional","affiliation":[{"name":"Department of Food Science and Human Nutrition, Laboratory of Dairy Research, Agricultural University of Athens, 75 Iera Odos Street, 11855 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolaos","family":"Moustridis","sequence":"additional","affiliation":[{"name":"Department of Food Science and Human Nutrition, Laboratory of Dairy Research, Agricultural University of Athens, 75 Iera Odos Street, 11855 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Masoura","sequence":"additional","affiliation":[{"name":"Department of Food Science and Human Nutrition, Laboratory of Dairy Research, Agricultural University of Athens, 75 Iera Odos Street, 11855 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5419-1290","authenticated-orcid":false,"given":"Theofilos","family":"Massouras","sequence":"additional","affiliation":[{"name":"Department of Food Science and Human Nutrition, Laboratory of Dairy Research, Agricultural University of Athens, 75 Iera Odos Street, 11855 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.5187\/jast.2021.e98","article-title":"Performance of Hanwoo calves fed a commercial colostrum replacer versus natural bovine colostrum","volume":"63","author":"Ahmadi","year":"2021","journal-title":"J. Anim. Sci. Technol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1093\/af\/vfad012","article-title":"The importance of colostrum in maternal care and its formation in mammalian species","volume":"13","author":"Baumrucker","year":"2023","journal-title":"Anim. Front."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/s43014-022-00104-1","article-title":"Bioactive compounds, nutritional profile and health benefits of colostrum: A review","volume":"4","author":"Poonia","year":"2022","journal-title":"Food Prod. Process. Nutr."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/0921-4488(95)00697-3","article-title":"Composition of ewe, goat and cow milk and of colostrum of ewes and goats","volume":"18","author":"Hadjipanayiotou","year":"1995","journal-title":"Small Rumin. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1007\/s11259-025-10646-7","article-title":"Deciphering the colostral-immunity transfer: From mammary gland to neonates small intestine","volume":"49","author":"Abdelrahman","year":"2025","journal-title":"Vet. Res. Commun."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"104624","DOI":"10.1016\/j.livsci.2021.104624","article-title":"Lambs need colostrum: A review","volume":"251","author":"Agenbag","year":"2021","journal-title":"Livest. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2320726","DOI":"10.1080\/10495398.2024.2320726","article-title":"Biochemical properties of sheep colostrum and its potential benefits for lamb survival: A review","volume":"35","author":"Farooq","year":"2024","journal-title":"Anim. Biotechnol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Flis, Z., Molik, E., Ptak, A., and Szatkowski, P. (2025). Natural Bioactive Compounds in Sheep Milk: Potential Biomedical Applications. Curr. Issues Mol. Biol., 47.","DOI":"10.3390\/cimb47060456"},{"key":"ref_9","first-page":"3706","article-title":"Colostrum composition of Santa Ines sheep and passive transfer of immunity to lambs","volume":"98","author":"Alves","year":"2015","journal-title":"J. Dairy Sci."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Abuelo, A., Cullens, F., Hanes, A., and Brester, J.L. (2021). Impact of 2 Versus 1 Colostrum Meals on Failure of Transfer of Passive Immunity, Pre-Weaning Morbidity and Mortality, and Performance of Dairy Calves in a Large Dairy Herd. Animals, 11.","DOI":"10.3390\/ani11030782"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e297","DOI":"10.1002\/vetr.5922","article-title":"Influence of ewe metabolic status on failure of passive transfer of immunity and lamb production in a UK lowland flock","volume":"197","author":"Kelly","year":"2025","journal-title":"Vet. Rec."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3521","DOI":"10.3168\/jds.2019-17397","article-title":"Risk factors associated with failed transfer of passive immunity in male and female dairy calves: A 2008 retrospective cross-sectional study","volume":"103","author":"Renaud","year":"2020","journal-title":"J. Dairy Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1064","DOI":"10.2460\/ajvr.1983.44.06.1064","article-title":"Failure in passive transfer of immunoglobulin G1 to lambs: Measurement of immunoglobulin G1 in ewe colostrums","volume":"44","author":"McGuire","year":"1983","journal-title":"Am. J. Vet. Res."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Cao, X., Ren, Y., Lu, Q., Wang, K., Wu, Y., Wang, Y., Zhang, Y., Cui, X.S., Yang, Z., and Chen, Z. (2022). Lactoferrin: A glycoprotein that plays an active role in human health. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.1018336"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1007\/s10534-022-00477-3","article-title":"Bovine lactoferrin for the prevention of COVID-19 infection in health care personnel: A double-blinded randomized clinical trial (LF-COVID)","volume":"36","author":"Navarro","year":"2023","journal-title":"Biometals"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1626","DOI":"10.1080\/10942912.2019.1666137","article-title":"Lactoferrin (LF): A natural antimicrobial protein","volume":"22","author":"Niaz","year":"2019","journal-title":"Int. J. Food Prop."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Playford, R.J., Weiser, M.J., and Marchbank, T. (2021). Methods to improve efficacy of orally administered bioactive peptides using bovine colostrum as an exemplar. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0253422"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1080\/10408398.2017.1381583","article-title":"Lactoferrin: Structure, function, denaturation and digestion","volume":"59","author":"Wang","year":"2019","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"111228","DOI":"10.1016\/j.biopha.2021.111228","article-title":"The role of iron in the pathogenesis of COVID-19 and possible treatment with lactoferrin and other iron chelators","volume":"136","author":"Habib","year":"2021","journal-title":"Biomed. Pharmacother. = Biomed. Pharmacother."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.actbio.2022.03.012","article-title":"Genetically engineered pH-responsive silk sericin nanospheres with efficient therapeutic effect on ulcerative colitis","volume":"144","author":"Xu","year":"2022","journal-title":"Acta Biomater."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1039\/D1FO02750F","article-title":"The effect of lactoferrin in aging: Role and potential","volume":"13","author":"Li","year":"2022","journal-title":"Food Funct."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.lfs.2018.01.002","article-title":"Multifunctional capacity and therapeutic potential of lactoferrin","volume":"195","author":"Ruiz","year":"2018","journal-title":"Life Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1097\/MPG.0000000000002908","article-title":"The Gut Microbiome and the Triple Environmental Hit Concept of Inflammatory Bowel Disease Pathogenesis","volume":"71","author":"Kellermayer","year":"2020","journal-title":"J. Pediatr. Gastroenterol. Nutr."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Triantaphyllopoulos, K.A., Ragia, N.D., Panagiotopoulou, M.E., and Sourlingas, T.G. (2025). Integrating Inflammatory and Epigenetic Signatures in IBD-Associated Colorectal Carcinogenesis: Models, Mechanisms, and Clinical Implications. Int. J. Mol. Sci., 26.","DOI":"10.20944\/preprints202508.1820.v1"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Todaro, M., Maniaci, G., Gannuscio, R., Pampinella, D., and Scatassa, M.L. (2023). Chemometric Approaches to Analyse the Composition of a Ewe\u2019s Colostrum. Animals, 13.","DOI":"10.3390\/ani13060983"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/0958-6946(94)90058-2","article-title":"Composition of colostrum from goats, ewes and cows producing twins","volume":"4","author":"Martin","year":"1994","journal-title":"Int. Dairy J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.nutos.2022.07.001","article-title":"Colostrum ingredients, its nutritional and health benefits\u2014An overview","volume":"44","year":"2022","journal-title":"Clin. Nutr. Open Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.smallrumres.2019.07.009","article-title":"Sheep milk components: Focus on nutritional advantages and biofunctional potential","volume":"180","author":"Moatsou","year":"2019","journal-title":"Small Rumin. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e70014","DOI":"10.1111\/1471-0307.70014","article-title":"Colostrum composition of Karya and \u00c7ine \u00c7apar\u0131 sheep: Variations in fatty acid and protein profiles during early lactation","volume":"78","author":"Durmaz","year":"2025","journal-title":"Int. J. Dairy Technol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Guiso, M.F., Battacone, G., Canu, L., Deroma, M., Langasco, I., Sanna, G., Tsiplakou, E., Pulina, G., and Nudda, A. (2022). Essential and Toxic Mineral Content and Fatty Acid Profile of Colostrum in Dairy Sheep. Animals, 12.","DOI":"10.3390\/ani12202730"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.idairyj.2011.07.006","article-title":"The lipid component of Massese ewes\u2019 colostrum: Morphometric characteristics of milk fat globules and fatty acid profile","volume":"24","author":"Martini","year":"2012","journal-title":"Int. Dairy J."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1016\/j.idairyj.2010.02.008","article-title":"Variation in fatty acid composition of ewes\u2019 colostrum and mature milk fat","volume":"20","author":"Kubinec","year":"2010","journal-title":"Int. Dairy J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.smallrumres.2018.09.002","article-title":"Lactoferrin and IgG levels in ovine milk throughout lactation: Correlation with milk quality parameters","volume":"168","author":"Navarro","year":"2018","journal-title":"Small Rumin. Res."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Zhang, X., Liu, X., Li, F., and Yue, X. (2020). The Differential Composition of Whey Proteomes in Hu Sheep Colostrum and Milk during Different Lactation Periods. Animals, 10.","DOI":"10.3390\/ani10101784"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"107750","DOI":"10.1016\/j.smallrumres.2026.107750","article-title":"Factors influencing small ruminant colostrum quality: A comprehensive review from 2020 to present","volume":"259","author":"DiGiacomo","year":"2026","journal-title":"Small Rumin. Res."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Puppel, K., Golebiewski, M., Grodkowski, G., Slosarz, J., Kunowska-Slosarz, M., Solarczyk, P., Lukasiewicz, M., Balcerak, M., and Przysucha, T. (2019). Composition and Factors Affecting Quality of Bovine Colostrum: A Review. Animals, 9.","DOI":"10.3390\/ani9121070"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"744","DOI":"10.56093\/ijans.v95i8.167679","article-title":"Nutritional and biochemical composition of colostrum and milk in sheep of the arid Region","volume":"95","author":"Vyas","year":"2026","journal-title":"Indian J. Anim. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Banos, G., Clark, E.L., Bush, S.J., Dutta, P., Bramis, G., Arsenos, G., Hume, D.A., and Psifidi, A. (2019). Genetic and genomic analyses underpin the feasibility of concomitant genetic improvement of milk yield and mastitis resistance in dairy sheep. PLoS ONE, 14.","DOI":"10.1101\/577015"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Ipharraguerre, I.R., Rosales Cavaglieri, L.A., and Rimbach, G. (2025). Bioenergetic signatures of circulating blood cells as biosensors of metabolic health in food-producing animals. Front. Vet. Sci., 12.","DOI":"10.3389\/fvets.2025.1653669"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Conte, G., Palombo, V., Serra, A., Correddu, F., D\u2019Andrea, M., Macciotta, N.P.P., and Mele, M. (2022). Study of the Fatty Acid Profile of Milk in Different Sheep Breeds: Evaluation by Multivariate Factorial Analysis. Animals, 12.","DOI":"10.3390\/ani12060722"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1007\/s13594-015-0234-5","article-title":"Assessment of lactation stage and breed effect on sheep milk fatty acid profile and lipid quality indices","volume":"95","author":"Sinanoglou","year":"2015","journal-title":"Dairy Sci. Technol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"9448","DOI":"10.1038\/s41598-021-88976-0","article-title":"Expression patterns of major genes in fatty acid synthesis, inflammation, oxidative stress pathways from colostrum to milk in Damascus goats","volume":"11","author":"Yakan","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"239","DOI":"10.5398\/tasj.2023.46.2.239","article-title":"Fatty Acid Composition and Nutritional Indices\/Ratios of Colostrum and Milk from Crossbred Goats in the Philippines","volume":"46","author":"Bondoc","year":"2023","journal-title":"Trop. Anim. Sci. J."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"5065","DOI":"10.3168\/jds.2013-7517","article-title":"Bovine colostrum: Changes in lipid constituents in the first 5 days after parturition","volume":"97","author":"Contarini","year":"2014","journal-title":"J. Dairy Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"3075","DOI":"10.3168\/jds.2025-27695","article-title":"A multivariate approach to exploring interrelationships among milk fatty acids across ruminant species","volume":"109","author":"Conte","year":"2026","journal-title":"J. Dairy Sci."},{"key":"ref_46","first-page":"1628","article-title":"Variations in fatty acid composition of Laoshan goat milk from partum to 135 days postpartum","volume":"89","author":"Lou","year":"2018","journal-title":"Anim. Sci. J. = Nihon Chikusan Gakkaiho"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"28","DOI":"10.17221\/5481-CJAS","article-title":"Changes in the composition of goat colostrum and milk fatty acids during the first month of lactation","volume":"57","author":"Marounek","year":"2012","journal-title":"Czech J. Anim. Sci."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Sinanoglou, V.J., Cavouras, D., Boutsikou, T., Briana, D.D., Lantzouraki, D.Z., Paliatsiou, S., Volaki, P., Bratakos, S., Malamitsi-Puchner, A., and Zoumpoulakis, P. (2017). Factors affecting human colostrum fatty acid profile: A case study. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0175817"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3617","DOI":"10.3168\/jds.2020-19462","article-title":"Metabolic and hormonal control of energy utilization and partitioning from early to mid lactation in Sarda ewes and Saanen goats","volume":"104","author":"Lunesu","year":"2021","journal-title":"J. Dairy Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"7054","DOI":"10.3168\/jds.2014-8039","article-title":"Milk fatty acids as possible biomarkers to early diagnose elevated concentrations of blood plasma nonesterified fatty acids in dairy cows","volume":"97","author":"Jorjong","year":"2014","journal-title":"J. Dairy Sci."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Mu, T., Hu, H., Ma, Y., Feng, X., Zhang, J., and Gu, Y. (2021). Regulation of Key Genes for Milk Fat Synthesis in Ruminants. Front. Nutr., 8.","DOI":"10.3389\/fnut.2021.765147"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1016\/0140-6736(91)91846-M","article-title":"Coronary heart disease: Seven dietary factors","volume":"338","author":"Ulbricht","year":"1991","journal-title":"Lancet"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"444S","DOI":"10.1093\/jn\/128.2.444S","article-title":"Dietary fatty acids and the regulation of plasma low density lipoprotein cholesterol concentrations","volume":"128","author":"Dietschy","year":"1998","journal-title":"J. Nutr."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.foodchem.2013.09.009","article-title":"Atherogenicity index and health-related fatty acids in different stages of lactation from Friesian, Jersey and FriesianxJersey cross cow milk under a pasture-based dairy system","volume":"146","author":"Nantapo","year":"2014","journal-title":"Food Chem."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Yang, C., Du, M., Ahmad, A.A., Cheng, Y., and Gebeyew, K. (2025). Passive Immunity Establishment Through Colostral IgG Absorption in Neonatal Ruminants: Foundation for Efficient Ruminant Production. Animals, 15.","DOI":"10.3390\/ani15213093"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/s12711-018-0378-x","article-title":"Genomic selection signatures in sheep from the Western Pyrenees","volume":"50","author":"Langa","year":"2018","journal-title":"Genet. Sel. Evol. GSE"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"601","DOI":"10.3168\/jds.S0022-0302(97)75976-9","article-title":"Genetic Parameters for Linear Udder Traits of Dairy Ewes","volume":"80","author":"Baro","year":"1997","journal-title":"J. Dairy Sci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"3672","DOI":"10.3168\/jds.2008-1111","article-title":"Quantitative Trait Loci Underlying Udder Morphology Traits in Dairy Sheep","volume":"91","author":"Alvarez","year":"2008","journal-title":"J. Dairy Sci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2238","DOI":"10.3168\/jds.S0022-0302(05)72899-X","article-title":"Genetic Parameters of Udder Traits, Somatic Cell Score, and Milk Yield in Latxa Sheep","volume":"88","author":"Legarra","year":"2005","journal-title":"J. Dairy Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2087","DOI":"10.3168\/jds.S0022-0302(85)81072-9","article-title":"Udder and teat morphology related to mastitis resistance: A review","volume":"68","author":"Seykora","year":"1985","journal-title":"J. Dairy Sci."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1017\/S0022029918000407","article-title":"Association of subclinical mastitis prevalence with sheep breeds in Greece","volume":"85","author":"Vasileiou","year":"2018","journal-title":"J. Dairy Res."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Piras, C., Ceniti, C., Hartmane, E., Costanzo, N., Morittu, V.M., Roncada, P., Britti, D., and Cramer, R. (2020). Rapid Liquid AP-MALDI MS Profiling of Lipids and Proteins from Goat and Sheep Milk for Speciation and Colostrum Analysis. Proteomes, 8.","DOI":"10.3390\/proteomes8030020"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"109037","DOI":"10.1016\/j.jfca.2026.109037","article-title":"Recent advances in the authentication of goat milk and its derived products","volume":"152","author":"Chaji","year":"2026","journal-title":"J. Food Compos. Anal."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Duli\u0144ski, R., Gancarz, M., Shakhovska, N., and Byczy\u0144ski, \u0141. (2025). The Application of Fourier Transform Infrared Spectroscopy and Chemometrics in Identifying Signatures for Sheep\u2019s Milk Authentication. Processes, 13.","DOI":"10.3390\/pr13020518"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Guerrero, L., Lamas, J.R., Mata, L., Razquin, P., Corrales, F., and Paradela, A. (2026). Qualitative and quantitative analysis of mixed-milk cheeses by targeted proteomics. J. Dairy Sci.","DOI":"10.3168\/jds.2025-27330"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"442","DOI":"10.3390\/nu3040442","article-title":"Perspectives on immunoglobulins in colostrum and milk","volume":"3","author":"Hurley","year":"2011","journal-title":"Nutrients"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Mafra, I., Honrado, M., and Amaral, J.S. (2022). Animal Species Authentication in Dairy Products. Foods, 11.","DOI":"10.3390\/foods11081124"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1071\/AN19305","article-title":"Fatty acid profile, antioxidative status and dietary value of the breast muscle of broiler chickens receiving glycine-Zn chelates","volume":"60","author":"Kwiatkowska","year":"2020","journal-title":"Anim. Prod. Sci."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Xu, M., Ma, B., Zhu, K., Tu, W., Li, C., Hao, P., and Zhang, M. (2026). Research Progress in Multi-Omics Analysis of Dairy Products: Nutritional Quality, Safety Evaluation, and Health Functions. Foods, 15.","DOI":"10.3390\/foods15132389"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"105741","DOI":"10.1016\/j.jff.2023.105741","article-title":"Prebiotic and modulatory evidence of lactoferrin on gut health and function","volume":"108","author":"Zhao","year":"2023","journal-title":"J. Funct. Foods"},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Triantaphyllopoulos, K.A. (2023). Long Non-Coding RNAs and Their \u201cDiscrete\u201d Contribution to IBD and Johne\u2019s Disease-What Stands out in the Current Picture? A Comprehensive Review. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241713566"},{"key":"ref_72","first-page":"301.e1","article-title":"Lactoferrin: Structure, function and applications","volume":"33","year":"2009","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Telang, S. (2018). Lactoferrin: A Critical Player in Neonatal Host Defense. Nutrients, 10.","DOI":"10.3390\/nu10091228"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1007\/s11250-023-03754-6","article-title":"Review: Genetic background of milk fatty acid synthesis in bovines","volume":"55","author":"Singh","year":"2023","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"105208","DOI":"10.1016\/j.jff.2022.105208","article-title":"Transcriptional regulation of milk fat synthesis in dairy cattle","volume":"96","author":"Tian","year":"2022","journal-title":"J. Funct. Foods"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/s40104-022-00823-y","article-title":"Rumen microbial-driven metabolite from grazing lambs potentially regulates body fatty acid metabolism by lipid-related genes in liver","volume":"14","author":"Li","year":"2023","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_77","first-page":"223","article-title":"Effects of breed on milk fatty acid profile in dairy ewes, with particular reference to cis-9, trans-11 conjugated linoleic acid","volume":"41","author":"Mierlita","year":"2011","journal-title":"South Afr. J. Anim. Sci."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"4692","DOI":"10.3168\/jds.2022-20880a","article-title":"Fatty acid profile characterization in colostrum, transition milk, and mature milk of primi- and multiparous cows during the first week of lactation","volume":"105","author":"Wilms","year":"2022","journal-title":"J. Dairy Sci."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"1989","DOI":"10.1038\/s41598-023-28966-6","article-title":"Dynamics of fatty acid and non-volatile polar metabolite profiles in colostrum and milk depending on the lactation stage and parity number of sows","volume":"13","author":"Settachaimongkon","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1186\/s40104-015-0026-z","article-title":"The scope for manipulating the polyunsaturated fatty acid content of beef: A review","volume":"6","author":"Vahmani","year":"2015","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Chen, J., and Liu, H. (2020). Nutritional Indices for Assessing Fatty Acids: A Mini-Review. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21165695"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.aquaculture.2017.12.043","article-title":"A blend of land animal fats can replace up to 75% fish oil without affecting growth and nutrient utilization of European seabass","volume":"487","author":"Monteiro","year":"2018","journal-title":"Aquaculture"},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Omri, B., Chalghoumi, R., Izzo, L., Ritieni, A., Lucarini, M., Durazzo, A., Abdouli, H., and Santini, A. (2019). Effect of Dietary Incorporation of Linseed Alone or Together with Tomato-Red Pepper Mix on Laying Hens\u2019 Egg Yolk Fatty Acids Profile and Health Lipid Indexes. Nutrients, 11.","DOI":"10.3390\/nu11040813"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"2596","DOI":"10.3168\/jds.2006-875","article-title":"Butter composition and texture from cows with different milk fatty acid compositions fed fish oil or roasted soybeans","volume":"90","author":"Bobe","year":"2007","journal-title":"J. Dairy Sci."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"2074","DOI":"10.1017\/S1751731116001130","article-title":"Effects of ewes grazing sulla or ryegrass pasture for different daily durations on forage intake, milk production and fatty acid composition of cheese","volume":"10","author":"Bonanno","year":"2016","journal-title":"Animal"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"6790","DOI":"10.3168\/jds.2018-15857","article-title":"Comparison of cheeses from goats fed 7 forages based on a new health index","volume":"102","author":"Giorgio","year":"2019","journal-title":"J. Dairy Sci."},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"Hadrova, S., Sedlakova, K., Krizova, L., and Malyugina, S. (2021). Alternative and Unconventional Feeds in Dairy Diets and Their Effect on Fatty Acid Profile and Health Properties of Milk Fat. Animals, 11.","DOI":"10.3390\/ani11061817"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"2646","DOI":"10.1007\/s13197-014-1303-0","article-title":"Effects of smoking and sun-drying on proximate, fatty and amino acids compositions of Southern pink shrimp (Penaeus notialis)","volume":"52","author":"Akintola","year":"2015","journal-title":"J. Food Sci. Technol."},{"key":"ref_89","doi-asserted-by":"crossref","unstructured":"Potocnik, D., Strojnik, L., Eftimov, T., Levart, A., and Ogrinc, N. (2020). Fatty Acid and Stable Carbon Isotope Composition of Slovenian Milk: Year, Season, and Regional Variability. Molecules, 25.","DOI":"10.3390\/molecules25122892"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.foodchem.2018.02.041","article-title":"Fatty acid profile of milk from Saanen and Swedish Landrace goats","volume":"254","author":"Yurchenko","year":"2018","journal-title":"Food Chem."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.idairyj.2017.06.007","article-title":"Chemical composition, protein fraction and fatty acid profile of donkey milk during lactation","volume":"75","author":"Massouras","year":"2017","journal-title":"Int. Dairy J."},{"key":"ref_93","first-page":"2825","article-title":"Scikit-learn: Machine Learning in Python","volume":"12","author":"Pedregosa","year":"2011","journal-title":"J. Mach. Learn. Res."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.6009\/jjrt.2023-2266","article-title":"Basics 5. Python+scikit-learn to try with medical image machine learning","volume":"79","author":"Tanaka","year":"2023","journal-title":"Jpn. J. Radiol. Technol."},{"key":"ref_95","doi-asserted-by":"crossref","unstructured":"Tang, D., Chen, M., Huang, X., Zhang, G., Zeng, L., Wu, S., and Wang, Y. (2023). SRplot: A free online platform for data visualization and graphing. PLoS ONE, 18.","DOI":"10.1371\/journal.pone.0294236"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/16\/7252\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T09:06:01Z","timestamp":1786698361000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/16\/7252"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,14]]},"references-count":95,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["ijms27167252"],"URL":"https:\/\/doi.org\/10.3390\/ijms27167252","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,14]]}}}