{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T04:26:31Z","timestamp":1786854391239,"version":"build-2736575974"},"reference-count":40,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Chang Gung Medical Research Program","award":["CMRPG2G0061"],"award-info":[{"award-number":["CMRPG2G0061"]}]},{"name":"Chang Gung Medical Research Program","award":["CMRPG2G0062"],"award-info":[{"award-number":["CMRPG2G0062"]}]},{"name":"Chang Gung Medical Research Program","award":["CMRPG2G0063"],"award-info":[{"award-number":["CMRPG2G0063"]}]},{"award":["CMRPG2G0061"],"award-info":[{"award-number":["CMRPG2G0061"]}],"id":[{"id":"https:\/\/ror.org\/02verss31","id-type":"ROR","asserted-by":"publisher"}]},{"award":["CMRPG2G0062"],"award-info":[{"award-number":["CMRPG2G0062"]}],"id":[{"id":"https:\/\/ror.org\/02verss31","id-type":"ROR","asserted-by":"publisher"}]},{"award":["CMRPG2G0063"],"award-info":[{"award-number":["CMRPG2G0063"]}],"id":[{"id":"https:\/\/ror.org\/02verss31","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Ministry of Science and Technology","award":["MOST 108-2314-B-182-049-MY3"],"award-info":[{"award-number":["MOST 108-2314-B-182-049-MY3"]}]},{"award":["MOST 108-2314-B-182-049-MY3"],"award-info":[{"award-number":["MOST 108-2314-B-182-049-MY3"]}],"id":[{"id":"https:\/\/ror.org\/02kv4zf79","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>The corneal epithelium serves as the outermost transparent barrier of the eye and depends on rapid and coordinated cellular responses for wound repair. Although negative pressure (NP) has been shown to accelerate wound healing in other tissues, its cellular and molecular effects on corneal epithelium remain undefined. This study investigates how NP regulates corneal epithelial cell physiology and identifies the molecular mechanisms underlying NP-induced migration. Human corneal epithelial cells were exposed to normal or NP conditions, and cell motility was quantified using scratch-wound and transwell migration assays. Nuclear and cytoplasmic fractions were isolated for proteomic profiling to identify NP-responsive proteins. G3BP1 was selected as a candidate regulator and subsequently examined using molecular, biochemical, and functional assays to determine its role in NP-mediated signaling. Proteomic analysis revealed a significant NP-induced upregulation of G3BP1. Mechanistically, G3BP1 suppressed epithelial junctional proteins, including E-cadherin, p120-catenin, and ZO-1, while activating key pro-migratory signaling pathways involving AKT, ERK1\/2, FAK, and Paxillin. These coordinated changes enhanced cytoskeletal dynamics and promoted corneal epithelial cell migration under NP stimulation. G3BP1 functions as a critical mechanotransduction mediator of NP, orchestrating adhesion remodeling and activating pro-migratory signaling cascades to facilitate corneal epithelial cell motility. These findings reveal a previously unrecognized cellular mechanism through which NP promotes epithelial repair and highlight G3BP1 as a potential therapeutic target for persistent corneal epithelial defects.<\/jats:p>","DOI":"10.3390\/ijms27167273","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T13:12:56Z","timestamp":1786713176000},"page":"7273","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Negative Pressure Promotes G3BP1-Mediated Migration of Corneal Epithelial Cells Through Activation of AKT\/ERK\/Paxillin Pathway"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-6700-7773","authenticated-orcid":false,"given":"Chia-Hui","family":"Lai","sequence":"first","affiliation":[{"name":"Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6873-6434","authenticated-orcid":false,"given":"Pang-Hung","family":"Hsu","sequence":"additional","affiliation":[{"name":"Department of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung 202, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5558-3456","authenticated-orcid":false,"given":"Chih-Chin","family":"Hsu","sequence":"additional","affiliation":[{"name":"Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital at Keelung, Keelung 204, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chien-Tzung","family":"Chen","sequence":"additional","affiliation":[{"name":"Division of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital at Keelung, Keelung 204, Taiwan"},{"name":"Department of Plastic and Reconstructive Surgery, Craniofacial Research Center, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1747-2226","authenticated-orcid":false,"given":"Yu-Chiau","family":"Shyu","sequence":"additional","affiliation":[{"name":"Department of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung 202, Taiwan"},{"name":"Community Medicine Research Center, Chang Gung Memorial Hospital, Keelung Branch, Keelung 204, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong-Hwei","family":"Su Pang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi-Chin","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Chang Gung Memorial Hospital at Keelung, Keelung 204, Taiwan"},{"name":"School of Medicine, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/bs.pmbts.2015.04.001","article-title":"Overview of the Cornea: Structure, Function, and Development","volume":"134","author":"Eghrari","year":"2015","journal-title":"Prog. 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