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J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Xyloglucan endotransglucosylase\/hydrolases (XTHs) are key cell-wall-remodeling enzymes that mediate xyloglucan modification, cell expansion, and organ elongation, yet the composition and variation in the XTH gene family across tea accessions remain unclear. In this study, we systematically identified and comparatively analyzed the XTH gene family across the genomes of nine tea accessions, yielding 477 putative XTH genes. Phylogenetic, conserved-motif, protein-domain, and conserved catalytic-motif analyses indicated that the major structural features of tea XTH proteins are broadly conserved, whereas orthogroup-based presence\u2013absence variation (PAV) analysis revealed core, variable, and accession-specific components within the family. Using transcriptomic expression profiles across eight tissues, we selected eight CsXTH genes for internode expression analysis. Reverse transcription quantitative PCR (RT-qPCR) showed that all eight genes were expressed at significantly higher levels in the third internode (I3) than in the second internode (I2) in \u2018Longjing 43\u2019 (LJ43), and CsXTH27 retained the same internode-associated expression pattern in \u2018Baihaozao\u2019 (BHZ) and \u2018Zhongcha 108\u2019 (ZC108), supporting its selection for functional analysis. Transient overexpression of CsXTH27 significantly increased the lengths of I2, I3, and I4, as well as the total length of I1\u2013I4, compared with the empty-vector control. This phenotype was accompanied by significantly increased XTH protein content in stems and an apparent tendency toward larger pith parenchyma cell profiles. Overall, these findings reveal both structural conservation and accession-level variation in the tea XTH gene family and provide preliminary functional evidence linking CsXTH27 to shoot internode elongation.<\/jats:p>","DOI":"10.3390\/ijms27167388","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:12:11Z","timestamp":1787069531000},"page":"7388","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Genomics of the Tea XTH Gene Family Reveals the Involvement of CsXTH27 in Internode Elongation"],"prefix":"10.3390","volume":"27","author":[{"given":"Hong","family":"Shen","sequence":"first","affiliation":[{"name":"College of Horticulture, Qingdao Agricultural University, Qingdao 266109, China"},{"name":"State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengshui","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China"},{"name":"College of Food Science, Southwest University, Chongqing 400715, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6702-8345","authenticated-orcid":false,"given":"Liping","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aihua","family":"Zhu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1079-189X","authenticated-orcid":false,"given":"Xin","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0814-2567","authenticated-orcid":false,"given":"Peiqiang","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Horticulture, Qingdao Agricultural University, Qingdao 266109, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuixing","family":"Zhu","sequence":"additional","affiliation":[{"name":"Agricultural Technology Promotion Center in Xianju County, Taizhou 317399, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Mi, X., Qiao, D., Yang, C., Chen, J., Liang, S., and Guo, Y. 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