{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T05:23:32Z","timestamp":1787203412750,"version":"build-2736575974"},"reference-count":55,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:00:00Z","timestamp":1787011200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Platelet-rich plasma (PRP) has emerged as one of the most extensively investigated orthobiologic therapies for tendon disorders because of its potential to modulate inflammation, enhance extracellular matrix remodeling, and promote tissue regeneration through the delivery of concentrated platelets and bioactive molecules. However, despite compelling biological rationale and encouraging preclinical evidence, clinical outcomes remain inconsistent across different tendon pathologies. This narrative review critically examines the principal biological and methodological factors underlying this variability, including differences in cellular composition, growth factor and cytokine profiles, activation strategies, and current PRP classification systems. We further synthesize the available clinical evidence across major tendon disorders, highlighting the influence of disease-specific biology, product heterogeneity, and procedural variability on treatment response. In addition, the emerging role of quantitative imaging biomarkers in objectively evaluating tendon regeneration is discussed as a complementary tool for biological outcome assessment. Based on the evidence reviewed, we propose the quantifiable platelet-rich plasma (Q-PRP) framework, a practical reporting model that integrates cellular, molecular, procedural, and clinical variables into a standardized approach for biologically meaningful PRP characterization. Rather than replacing existing classification systems, the proposed framework aims to improve reproducibility, facilitate cross-study comparison, and support the transition toward precision regenerative medicine. Standardized biological characterization, combined with objective outcome assessment, may represent a critical step toward optimizing PRP research and clinical application in tendon healing.<\/jats:p>","DOI":"10.3390\/ijms27167393","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:12:11Z","timestamp":1787069531000},"page":"7393","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Toward Standardized Platelet-Rich Plasma Therapy in Tendon Healing: Integrating Biological Characterization with Clinical Translation"],"prefix":"10.3390","volume":"27","author":[{"given":"Jeries Issa","family":"Alghishan","sequence":"first","affiliation":[{"name":"Department of Orthopedics and Traumatology, Victor Babe\u0219 University of Medicine and Pharmacy, 300723 Timi\u0219oara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9784-176X","authenticated-orcid":false,"given":"Bogdan","family":"Andor","sequence":"additional","affiliation":[{"name":"Department of Orthopedics and Traumatology, Victor Babe\u0219 University of Medicine and Pharmacy, 300723 Timi\u0219oara, Romania"},{"name":"Teodor \u0218ora Research Center, Victor Babe\u0219 University of Medicine and Pharmacy, 300723 Timi\u0219oara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5931","DOI":"10.2147\/JPR.S546655","article-title":"Biological Mechanisms and Clinical Challenges of Platelet-Rich Plasma in Chronic Musculoskeletal Pain: From Standardized Preparation to Multi-Omics-Guided Precision Therapy","volume":"18","author":"Zhou","year":"2025","journal-title":"J. Pain Res."},{"key":"ref_2","first-page":"e65636","article-title":"Mechanisms, Efficacy, and Clinical Applications of Platelet-Rich Plasma in Tendinopathy: A Comprehensive Review","volume":"16","author":"Kale","year":"2024","journal-title":"Cureus"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1007\/s12178-024-09922-x","article-title":"The Effect of Platelet Dose on Outcomes after Platelet Rich Plasma Injections for Musculoskeletal Conditions: A Systematic Review and Meta-Analysis","volume":"17","author":"Berrigan","year":"2024","journal-title":"Curr. Rev. Musculoskelet. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"104593","DOI":"10.1016\/j.amsu.2022.104593","article-title":"Standardization and Validation of a Conventional High Yield Platelet-Rich Plasma Preparation Protocol","volume":"82","author":"Muthu","year":"2022","journal-title":"Ann. Med. Surg."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Wu, W.S., Chen, L.R., and Chen, K.H. (2025). Platelet-Rich Plasma (PRP): Molecular Mechanisms, Actions and Clinical Applications in Human Body. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms262110804"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Xu, J., Du, W., Xue, X., Chen, M., Zhou, W., and Luo, X. (2023). Global Research Trends on Platelet-Rich Plasma for Tendon and Ligament Injuries from the Past Two Decades: A Bibliometric and Visualized Study. Front. Surg., 10.","DOI":"10.3389\/fsurg.2023.1113491"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1007\/s12178-018-9527-7","article-title":"Current Clinical Recommendations for Use of Platelet-Rich Plasma","volume":"11","author":"Le","year":"2018","journal-title":"Curr. Rev. Musculoskelet. Med."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Berdini, M., Clementi, G., Torcianti, M., Del Bianco, D., Cantori, I., Procaccini, R., and Gigante, A. (2026). Standardization of Platelet-Rich Plasma Preparation in Orthopedics: A Review of the Literature and Proposal for a Reproducible Protocol. LabMed, 3.","DOI":"10.3390\/labmed3010008"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Corsini, A., Perticarini, L., Palermi, S., Bettinsoli, P., and Marchini, A. (2025). Re-Evaluating Platelet-Rich Plasma Dosing Strategies in Sports Medicine: The Role of the \u201c10 Billion Platelet Dose\u201d in Optimizing Therapeutic Outcomes\u2014A Narrative Review. J. Clin. Med., 14.","DOI":"10.3390\/jcm14082714"},{"key":"ref_10","first-page":"60","article-title":"DEPA Classification: A Proposal for Standardising PRP Use and a Retrospective Application of Available Devices","volume":"2","author":"Magalon","year":"2016","journal-title":"Exerc. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1080\/08977194.2022.2082294","article-title":"Transforming Growth Factor-\u03b2 Signalling Pathway in Tendon Healing","volume":"40","author":"Li","year":"2022","journal-title":"Growth Factors"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3852898","DOI":"10.1155\/2022\/3852898","article-title":"Variability in Platelet-Rich Plasma Preparations Used in Regenerative Medicine: A Comparative Analysis","volume":"2022","author":"Tey","year":"2022","journal-title":"Stem Cells Int."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1302\/2058-5241.6.200017","article-title":"Platelet-Rich Plasma: A Narrative Review","volume":"6","author":"Collins","year":"2021","journal-title":"EFORT Open Rev."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Liu, X., Zhu, B., Li, Y., Liu, X., Guo, S., Wang, C., Li, S., and Wang, D. (2021). The Role of Vascular Endothelial Growth Factor in Tendon Healing. Front. Physiol., 12.","DOI":"10.3389\/fphys.2021.766080"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"311ra173","DOI":"10.1126\/scitranslmed.aac4269","article-title":"Inflammation Activation and Resolution in Human Tendon Disease","volume":"7","author":"Dakin","year":"2015","journal-title":"Sci. Transl. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1038\/nrrheum.2016.213","article-title":"Inflammatory Mechanisms in Tendinopathy\u2014Towards Translation","volume":"13","author":"Millar","year":"2017","journal-title":"Nat. Rev. Rheumatol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2135","DOI":"10.1177\/0363546511417792","article-title":"Growth Factor and Catabolic Cytokine Concentrations Are Influenced by the Cellular Composition of Platelet-Rich Plasma","volume":"39","author":"Sundman","year":"2011","journal-title":"Am. J. Sports Med."},{"key":"ref_18","first-page":"3","article-title":"Classification of Platelet Concentrates (Platelet-Rich Plasma-PRP, Platelet-Rich Fibrin-PRF) for Topical and Infiltrative Use in Orthopedic and Sports Medicine: Current Consensus, Clinical Implications and Perspectives","volume":"4","author":"Andia","year":"2014","journal-title":"Muscles Ligaments Tendons J."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Lu, J., Li, H., Zhang, Z., Xu, R., Wang, J., and Jin, H. (2023). Platelet-Rich Plasma in the Pathologic Processes of Tendinopathy: A Review of Basic Science Studies. Front. Bioeng. Biotechnol., 11.","DOI":"10.3389\/fbioe.2023.1187974"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e335","DOI":"10.1016\/j.asmr.2021.10.004","article-title":"Double-Spin Leukocyte-Rich Platelet-Rich Plasma Is Predominantly Lymphocyte Rich with Notable Concentrations of Other White Blood Cell Subtypes","volume":"4","author":"Marathe","year":"2022","journal-title":"Arthrosc. Sports Med. Rehabil."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"5289145","DOI":"10.1155\/2022\/5289145","article-title":"Leukocyte and Platelet-Rich Plasma (L-PRP) in Tendon Models: A Systematic Review and Meta-Analysis of In Vivo\/In Vitro Studies","volume":"2022","author":"Liu","year":"2022","journal-title":"Evid.-Based Complement. Altern. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1186\/s13018-025-06308-8","article-title":"The Effect of Platelet-Rich Plasma on Clinical Outcomes within Two Years after Anterior Cruciate Ligament Reconstruction: A Systematic Review and Meta-Analysis of Randomized Controlled Trials","volume":"20","author":"Li","year":"2025","journal-title":"J. Orthop. Surg. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1096\/fj.14-258236","article-title":"Scleraxis Is Required for the Development of a Functional Tendon Enthesis","volume":"30","author":"Killian","year":"2016","journal-title":"FASEB J."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1002\/jor.22806","article-title":"Mechanisms of Tendon Injury and Repair","volume":"33","author":"Thomopoulos","year":"2015","journal-title":"J. Orthop. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2097","DOI":"10.1007\/s00167-013-2680-z","article-title":"Tendon Healing: An Overview of Physiology, Biology, and Pathology of Tendon Healing and Systematic Review of State of the Art in Tendon Bioengineering","volume":"23","author":"Todorov","year":"2015","journal-title":"Knee Surg. Sports Traumatol. Arthrosc."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s11010-017-3097-7","article-title":"MicroRNAs Associated with Inflammation in Shoulder Tendinopathy and Glenohumeral Arthritis","volume":"437","author":"Thankam","year":"2018","journal-title":"Mol. Cell. Biochem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1136\/bjsports-2015-094754","article-title":"Are Inflammatory Cells Increased in Painful Human Tendinopathy? A Systematic Review","volume":"50","author":"Dean","year":"2016","journal-title":"Br. J. Sports Med."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Ackermann, P.W., Franklin, S.L., Dean, B.J.F., Carr, A.J., Salo, P.T., and Hart, D.A. (2014). Neuronal Pathways in Tendon Healing and Tendinopathy-Update. Front. Biosci., 19.","DOI":"10.2741\/4280"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1302\/2046-3758.612.BJR-2017-0112.R1","article-title":"Cytokines in Tendon Disease: A Systematic Review","volume":"6","author":"Morita","year":"2017","journal-title":"Bone Jt. Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.cveq.2011.05.001","article-title":"Autologous Biologic Treatment for Equine Musculoskeletal Injuries: Platelet-Rich Plasma and IL-1 Receptor Antagonist Protein","volume":"27","author":"Textor","year":"2011","journal-title":"Vet. Clin. N. Am.\u2014Equine Pract."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Mart\u00ednez, C.E., Smith, P.C., and Palma Alvarado, V.A. (2015). The Influence of Platelet-Derived Products on Angiogenesis and Tissue Repair: A Concise Update. Front. Physiol., 6.","DOI":"10.3389\/fphys.2015.00290"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1097\/ID.0b013e31819e8ec6","article-title":"Platelet-Rich Plasma: A Literature Review","volume":"18","author":"Arora","year":"2009","journal-title":"Implant. Dent."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/j.reth.2025.01.008","article-title":"Effect of Platelet-Rich Plasma on Angiogenic and Regenerative Properties in Patients with Critical Limb Ischemia","volume":"28","author":"Tanzadehpanah","year":"2025","journal-title":"Regen. Ther."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1742","DOI":"10.1177\/0363546512452713","article-title":"The Positive Effects of Different Platelet-Rich Plasma Methods on Human Muscle, Bone, and Tendon Cells","volume":"40","author":"Mazzocca","year":"2012","journal-title":"Am. J. Sports Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1177\/0363546510387517","article-title":"Comparison of Growth Factor and Platelet Concentration from Commercial Platelet-Rich Plasma Separation Systems","volume":"39","author":"Castillo","year":"2011","journal-title":"Am. J. Sports Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1080\/09537104.2017.1336211","article-title":"The Use of Platelet-Rich Plasma to Treat Chronic Tendinopathies: A Technical Analysis","volume":"29","author":"Kaux","year":"2018","journal-title":"Platelets"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"6444120","DOI":"10.1155\/2024\/6444120","article-title":"Current Challenges in the Development of Platelet-Rich Plasma-Based Therapies","volume":"2024","author":"Beitia","year":"2024","journal-title":"BioMed Res. Int."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1097\/00000658-200101000-00014","article-title":"Immunologic Impact and Clinical Outcomes After Surgical Exposure to Bovine Thrombin","volume":"233","author":"Ortel","year":"2001","journal-title":"Ann. Surg."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1016\/j.athoracsur.2003.09.110","article-title":"Antihuman Factor V Antibodies after Use of Relatively Pure Bovine Thrombin","volume":"79","author":"Lawson","year":"2005","journal-title":"Ann. Thorac. Surg."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"e25","DOI":"10.1136\/bjsm.2004.015164","article-title":"High Strain Mechanical Loading Rapidly Induces Tendon Apoptosis: An Ex Vivo Rat Tibialis Anterior Model","volume":"39","author":"Scott","year":"2005","journal-title":"Br. J. Sports Med."},{"key":"ref_41","first-page":"umae017","article-title":"Platelet-Rich Plasma for Patellar Tendinopathy: A Randomized Controlled Trial Correlating Clinical Outcomes and Quantitative Imaging","volume":"1","author":"Stewart","year":"2024","journal-title":"Radiol. Adv."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1177\/03635465231185289","article-title":"Randomized Controlled Trials on Platelet-Rich Plasma for Knee Osteoarthritis Poorly Adhere to the Minimum Information for Studies Evaluating Biologics in Orthopaedics (MIBO) Guidelines: A Systematic Review","volume":"52","author":"Stone","year":"2024","journal-title":"Am. J. Sports Med."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"17171","DOI":"10.1038\/s41598-020-74341-0","article-title":"Effects of Applying Platelet-Rich Plasma during Arthroscopic Rotator Cuff Repair: A Systematic Review and Meta-Analysis of Randomised Controlled Trials","volume":"10","author":"Yang","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1001\/jama.2021.6986","article-title":"Effect of Platelet-Rich Plasma Injection vs Sham Injection on Tendon Dysfunction in Patients with Chronic Midportion Achilles Tendinopathy: A Randomized Clinical Trial","volume":"326","author":"Kearney","year":"2021","journal-title":"JAMA"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1533","DOI":"10.1016\/j.jse.2022.02.017","article-title":"A Systematic Review on the Efficacy of Different Types of Platelet-Rich Plasma in the Management of Lateral Epicondylitis","volume":"31","author":"Li","year":"2022","journal-title":"J. Shoulder Elb. Surg."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Kizilkurt, T., Aydin, A.S., Yagci, T.F., Ersen, A., Ercan, C.C., and Salmaslioglu, A. (2025). Platelet-Rich Plasma Provides Superior Clinical Outcomes Without Radiologic Differences in Lateral Epicondylitis: Randomized Controlled Trial. Medicina, 61.","DOI":"10.3390\/medicina61050894"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Hamid, M.S.A., and Sazlina, S.G. (2021). Platelet-Rich Plasma for Rotator Cuff Tendinopathy: A Systematic Review and Meta-Analysis. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0251111"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"23259671241296508","DOI":"10.1177\/23259671241296508","article-title":"Effect of Platelet-Rich Plasma Injection on the Treatment of Achilles Tendinopathy: A Systematic Review and Meta-Analysis","volume":"12","author":"Ling","year":"2024","journal-title":"Orthop. J. Sports Med."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Ger\u0161ak, \u017d.M., Pintari\u0107, K., Vidmar, J., and Salapura, V. (2026). Quantitative Imaging Biomarkers of PRP-Induced Tendon Remodelling in Chronic Tendinopathy: Review and Single-Centre Experience with Ultrasound Radiomics and MRI T2 Profiling. Diagnostics, 16.","DOI":"10.3390\/diagnostics16081233"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1016\/j.arthro.2012.04.148","article-title":"Platelet-Rich Plasma: The PAW Classification System","volume":"28","author":"Delong","year":"2012","journal-title":"Arthroscopy"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"809","DOI":"10.2106\/JBJS.16.00793","article-title":"Minimum Information for Studies Evaluating Biologics in Orthopaedics (MIBO): Platelet-Rich Plasma and Mesenchymal Stem Cells","volume":"99","author":"Murray","year":"2017","journal-title":"J. Bone Jt. Surg."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Niemiec, P., Jarosz, A., Balcerzyk-Mati\u0107, A., Iwanicka, J., Nowak, T., Iwanicki, T., Gierek, M., Kalita, M., Garczorz, W., and Francuz, T. (2023). Genetic Variability in VEGFA Gene Influences the Effectiveness of Tennis Elbow Therapy with PRP: A Two-Year Prospective Cohort Study. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms242417292"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Jarosz, A., Wrona, J., Balcerzyk-Mati\u0107, A., Szyluk, K., Nowak, T., Iwanicki, T., Iwanicka, J., Kalita, M., Kania, W., and Gawron, K. (2025). Association of the TGFB1 Gene Polymorphisms with Pain Symptoms and the Effectiveness of Platelet-Rich Plasma in the Treatment of Lateral Elbow Tendinopathy: A Prospective Cohort Study. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26062431"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Jarosz, A., Balcerzyk-Mati\u0107, A., Iwanicka, J., Iwanicki, T., Nowak, T., Szyluk, K., Kalita, M., G\u00f3rczy\u0144ska-Kosiorz, S., Kania, W., and Niemiec, P. (2024). Association between Platelet-Derived Growth Factor Receptor Alpha Gene Polymorphisms and Platelet-Rich Plasma\u2019s Efficiency in Treating Lateral Elbow Tendinopathy\u2014A Prospective Cohort Study. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25084266"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Szyluk, K., Jarosz, A., Balcerzyk-Mati\u0107, A., Iwanicka, J., Iwanicki, T., Nowak, T., Gierek, M., Negru, M., Kalita, M., and G\u00f3rczy\u0144ska-Kosiorz, S. (2022). Polymorphic Variants of the PDGFRB Gene Influence Efficacy of PRP Therapy in Treating Tennis Elbow: A Prospective Cohort Study. J. Clin. Med., 11.","DOI":"10.3390\/jcm11216362"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/16\/7393\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T04:27:02Z","timestamp":1787200022000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/16\/7393"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,18]]},"references-count":55,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["ijms27167393"],"URL":"https:\/\/doi.org\/10.3390\/ijms27167393","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,18]]}}}