{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T05:23:28Z","timestamp":1787203408960,"version":"build-2736575974"},"reference-count":29,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:00:00Z","timestamp":1787011200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004539","name":"Kasetsart University","doi-asserted-by":"crossref","award":["Project 2025"],"award-info":[{"award-number":["Project 2025"]}],"id":[{"id":"10.13039\/501100004539","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004539","name":"Kasetsart University","doi-asserted-by":"crossref","award":["[FF(KU-SRIU)7.68]"],"award-info":[{"award-number":["[FF(KU-SRIU)7.68]"]}],"id":[{"id":"10.13039\/501100004539","id-type":"DOI","asserted-by":"crossref"}]},{"award":["Project 2025"],"award-info":[{"award-number":["Project 2025"]}],"id":[{"id":"https:\/\/ror.org\/05gzceg21","id-type":"ROR","asserted-by":"publisher"}]},{"award":["[FF(KU-SRIU)7.68]"],"award-info":[{"award-number":["[FF(KU-SRIU)7.68]"]}],"id":[{"id":"https:\/\/ror.org\/05gzceg21","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Feline kobuvirus (FKoV) has been linked to diarrheal disease in cats and has recently emerged in several Asian countries. This study aimed to investigate the epidemiological and genetic characterization of FKoV in domestic cats in northern Vietnam from 2022 to 2025. In total, 244 fecal samples were obtained from both healthy and sick cats in Hanoi, Hungyen, Bacninh, and Ninhbinh in the north of Vietnam. Of 244 fecal samples examined, eight (3.28%) samples were found to be positive for the FKoV genome by using the conventional PCR method. Viral genomes were identified in both healthy and sick cats. The highest FKoV-positive rate was 12.50% in 6\u201312-month-old cats, significantly higher than those detected in cats aged 3\u20136 months and &gt;12 months. Eight positive samples were successfully sequenced and characterized for the partial 3D gene and full-length VP1 genes. Results showed that the level of nucleotide identity of the partial 3D gene and full-length VP1 genes among the FKoV strains in this study ranged from 95.86% to 100% and 94.09% to 99.73%, respectively. Phylogenetic analysis revealed that the eight Vietnamese viral strains obtained in this study formed a novel cluster within FKoV and are genetically related to Chinese FKoV strains. Two positive selections were found in the VP1 protein of the Vietnamese FKoV strains.<\/jats:p>","DOI":"10.3390\/ijms27167394","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:12:11Z","timestamp":1787069531000},"page":"7394","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Feline Kobuvirus in Domestic Cats in Northern Vietnam: Molecular Insights Reveal Adaptive Evolution in VP1 Protein"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6340-9204","authenticated-orcid":false,"given":"Hieu Van","family":"Dong","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine, Vietnam National University of Agricuture, Hanoi 131000, Vietnam"},{"name":"Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0841-3358","authenticated-orcid":false,"given":"Giang Thi Huong","family":"Tran","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Vietnam National University of Agricuture, Hanoi 131000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoang Viet The","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Vietnam National University of Agricuture, Hanoi 131000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linh Ngoc","family":"Phan","sequence":"additional","affiliation":[{"name":"GAIA Pet Clinic Ocean Park, Gia Lam, Hanoi 131000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amonpun","family":"Rattanasrisomporn","sequence":"additional","affiliation":[{"name":"Interdisciplinary of Genetic Engineering and Bioinformatics, Graduate School, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaiwat","family":"Boonkaewwan","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Phetchaburi Rajabhat University, Phetchaburi 76000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4639-8945","authenticated-orcid":false,"given":"Dao Anh Tran","family":"Bui","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Vietnam National University of Agricuture, Hanoi 131000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2426-0961","authenticated-orcid":false,"given":"Jatuporn","family":"Rattanasrisomporn","sequence":"additional","affiliation":[{"name":"Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand"},{"name":"Interdisciplinary of Genetic Engineering and Bioinformatics, Graduate School, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2421","DOI":"10.1099\/jgv.0.000911","article-title":"ICTV virus taxonomy profile: Picornaviridae","volume":"98","author":"Zell","year":"2017","journal-title":"J. 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