{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T05:23:43Z","timestamp":1787289823832,"version":"build-2736575974"},"reference-count":64,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004442","name":"National Science Centre","doi-asserted-by":"crossref","award":["NN 311 55 8840"],"award-info":[{"award-number":["NN 311 55 8840"]}],"id":[{"id":"10.13039\/501100004442","id-type":"DOI","asserted-by":"crossref"}]},{"award":["NN 311 55 8840"],"award-info":[{"award-number":["NN 311 55 8840"]}],"id":[{"id":"https:\/\/ror.org\/03ha2q922","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Body condition score (BCS) is widely used to assess the energy status of dairy cows, yet its relationship with coordinated metabolic and immune responses during early lactation remains incompletely understood. The aim of this study was to characterize the physiological profile associated with low BCS using biomarkers of energy metabolism, hepatic function, oxidative status, inflammation, mammary gland health, milk production, and milk composition. A total of 580 Polish Holstein\u2013Friesian cows in early lactation were classified into four BCS categories and evaluated using univariate and multivariate statistical approaches, including composite physiological indices and partial least squares discriminant analysis (PLS-DA). Cows with the lowest BCS (2.0\u20132.5) exhibited marked lipid mobilization and hepatic stress, reflected by higher concentrations of non-esterified fatty acids, \u03b2-hydroxybutyrate, aspartate aminotransferase, and gamma-glutamyl transferase (all p &lt; 0.001). These changes were accompanied by increased oxidative stress, elevated inflammatory activity, alterations in mammary gland biomarkers, and changes in selected milk traits. Composite physiological indices consistently differentiated low-BCS cows from the remaining groups, while PLS-DA identified a distinct biomarker profile associated with low body condition. Among the evaluated variables, interleukin-8 showed the greatest contribution to class discrimination, ranking above conventional metabolic biomarkers in the multivariate analysis. The results indicate that low body condition score is associated with coordinated alterations involving metabolism, liver function, oxidative balance, inflammation, and mammary gland responses rather than isolated metabolic disturbances. The proposed composite physiological indices and the Integrated Low-BCS Phenotype Score (ILBPS) provide an integrative framework for characterizing the physiological phenotype associated with low body condition during early lactation. Prospective longitudinal studies are required to determine whether these biomarkers and composite indices have prognostic value for subsequent metabolic or clinical outcomes.<\/jats:p>","DOI":"10.3390\/ijms27167416","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T13:14:01Z","timestamp":1787145241000},"page":"7416","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Metabolic, Oxidative, Inflammatory, and Mammary Health Signatures Characterize Low Body Condition Score in Dairy Cows"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2909-2087","authenticated-orcid":false,"given":"Kamila","family":"Puppel","sequence":"first","affiliation":[{"name":"Institute of Animal Science, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Sl\u00f3sarz","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5715-6514","authenticated-orcid":false,"given":"Grzegorz","family":"Grodkowski","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7683-4998","authenticated-orcid":false,"given":"Pawe\u0142","family":"Solarczyk","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5413-0754","authenticated-orcid":false,"given":"Piotr","family":"Kostusiak","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma\u0142gorzata","family":"Kunowska-Sl\u00f3sarz","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7340-4100","authenticated-orcid":false,"given":"Karol","family":"Tucki","sequence":"additional","affiliation":[{"name":"Institute of Mechanical Engineering, Warsaw University of Life Sciences, Nowoursynowska 164, 02-787 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0202-3340","authenticated-orcid":false,"given":"Krzysztof","family":"M\u0142ynek","sequence":"additional","affiliation":[{"name":"Institute of Animal Science and Fisheries, University of Siedlce, ul. B. Prusa 14, 08-110 Siedlce, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marek","family":"Balcerak","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.anifeedsci.2005.08.003","article-title":"Feeding-and management-related diseases in the transition cow: Physiological adaptations around calving and strategies to reduce feeding-related diseases","volume":"126","author":"Ingvartsen","year":"2006","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"S29","DOI":"10.1262\/jrd.1056S29","article-title":"Monitoring metabolic health of dairy cattle in the transition period","volume":"56","author":"LeBlanc","year":"2010","journal-title":"J. Reprod. Dev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2820","DOI":"10.2527\/1995.7392820x","article-title":"Impact of changes in organic nutrient metabolism on feeding the transition dairy cow","volume":"73","author":"Grummer","year":"1995","journal-title":"J. Anim. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1596","DOI":"10.3168\/jds.2009-2852","article-title":"Associations of elevated nonesterified fatty acids and \u03b2-hydroxybutyrate concentrations with early lactation reproductive performance and milk production in transition dairy cattle in the northeastern United States","volume":"93","author":"Ospina","year":"2010","journal-title":"J. Dairy Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2259","DOI":"10.3168\/jds.S0022-0302(99)75474-3","article-title":"Biology of dairy cows during the transition period: The final frontier?","volume":"82","author":"Drackley","year":"1999","journal-title":"J. Dairy Sci."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Solarczyk, P., Go\u0142\u0119biewski, M., Sl\u00f3sarz, J., and Puppel, K. (2023). Interaction between the Concentration of \u03b2-Hydroxybutyric Acid and the Content of Long-Chain Fatty Acids in the Early Stage of Lactation\u2013\u2013Comparing Multiparous and Primiparous Cows. Appl. Sci., 13.","DOI":"10.3390\/app13137870"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Solarczyk, P., Go\u0142\u0119biewski, M., Sl\u00f3sarz, J., Natalello, A., Musati, M., Menci, R., Sakowski, T., Tucki, K., and Puppel, K. (2024). Effect of Age at First Calving on the Reproduction Parameters, Metabolic Profile, and Fatty Acid Composition of Polish Holstein Friesian (PHF) and Crossbreds PHF \u00d7 Swedish Red (SRB) Cattle. Metabolites, 14.","DOI":"10.3390\/metabo14110583"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1186\/s40104-020-00501-x","article-title":"Role of nutraceuticals during the transition period of dairy cows: A review","volume":"11","author":"Lopreiato","year":"2020","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"5769","DOI":"10.3168\/jds.2009-2431","article-title":"Invited review: Body condition score and its association with dairy cow productivity, health, and welfare","volume":"92","author":"Roche","year":"2009","journal-title":"J. Dairy Sci."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"M\u0142ynek, K., Wnorowska, K., Danielewicz, A., Natalello, A., and Puppel, K. (2025). Inversely correlated restoration of body condition score and systemic metabolic burden in lactating cows: Implications for milk fat globule size and mitigation of negative energy balance effects on milk fat composition. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26094296"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"4321","DOI":"10.1002\/jsfa.7779","article-title":"Metabolic profiles of cow\u2019s blood; a review","volume":"96","author":"Puppel","year":"2016","journal-title":"J. Sci. Food Agric."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.immuni.2017.08.009","article-title":"Foundations of immunometabolism and implications for metabolic health and disease","volume":"47","author":"Hotamisligil","year":"2017","journal-title":"Immunity"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1038\/nri.2016.70","article-title":"A guide to immunometabolism for immunologists","volume":"16","author":"Kishton","year":"2016","journal-title":"Nat. Rev. Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"6631","DOI":"10.3168\/jds.2015-9683","article-title":"Invited review: Inflammation during the transition to lactation: New adventures with an old flame","volume":"98","author":"Bradford","year":"2015","journal-title":"J. Dairy Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.cimid.2011.01.004","article-title":"Lipid mobilization and inflammatory responses during the transition period of dairy cows","volume":"34","author":"Contreras","year":"2011","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"51","DOI":"10.24425\/pjvs.2025.154013","article-title":"Investigation on body condition score, milk yield, reproductive performance, and health of dairy cows in four intensive dairy farms during lactation cycle","volume":"28","author":"Jiang","year":"2025","journal-title":"Pol. J. Vet. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"4131","DOI":"10.3168\/jds.2024-25425","article-title":"Associations among body condition score, body weight, and serum biochemistry in dairy cows","volume":"108","author":"Sheedy","year":"2025","journal-title":"J. Dairy Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.rvsc.2017.12.001","article-title":"Assessment of the innate immune response in the periparturient cow","volume":"116","author":"Trevisi","year":"2018","journal-title":"Res. Vet. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"5056","DOI":"10.3168\/jds.2012-5443","article-title":"Epidemiology of subclinical ketosis in early lactation dairy cattle","volume":"95","author":"McArt","year":"2012","journal-title":"J. Dairy Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"5011","DOI":"10.3168\/jds.2012-5382","article-title":"Nonesterified fatty acids modify inflammatory response and eicosanoid biosynthesis in bovine endothelial cells","volume":"95","author":"Contreras","year":"2012","journal-title":"J. Dairy Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/S0749-0720(15)30102-X","article-title":"Ruminant adaptation to negative energy balance: Influences on the etiology of ketosis and fatty liver","volume":"16","author":"Herdt","year":"2000","journal-title":"Vet. Clin. N. Am. Food Anim. Pract."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3105","DOI":"10.3168\/jds.S0022-0302(04)73446-3","article-title":"Invited review: Pathology, etiology, prevention, and treatment of fatty liver in dairy cows","volume":"87","author":"Bobe","year":"2004","journal-title":"J. Dairy Sci."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Puppel, K., Sl\u00f3sarz, J., Grodkowski, G., Solarczyk, P., Kostusiak, P., Kunowska-Sl\u00f3sarz, M., Grodkowska, K., Zalewska, A., Kuczy\u0144ska, B., and Go\u0142\u0119biewski, M. (2022). Comparison of enzyme activity in order to describe the metabolic profile of dairy cows during early lactation. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23179771"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"251","DOI":"10.4142\/jvs.2011.12.3.251","article-title":"Relationship among blood indicators of lipomobilization and hepatic function during early lactation in high-yielding dairy cows","volume":"12","author":"Pereira","year":"2011","journal-title":"J. Vet. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1146\/annurev-animal-031412-103728","article-title":"Systems physiology in dairy cattle: Nutritional genomics and beyond","volume":"1","author":"Loor","year":"2013","journal-title":"Ann. Rev. Anim. Biosci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1740","DOI":"10.3168\/jds.2006-445","article-title":"Plasma paraoxonase, health, inflammatory conditions, and liver function in transition dairy cows","volume":"90","author":"Bionaz","year":"2007","journal-title":"J. Dairy Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.cvfa.2013.03.002","article-title":"Significance of Metabolic Stress, Lipid Mobilization, and Inflammation on Transition Cow Disorders","volume":"29","author":"Sordillo","year":"2013","journal-title":"Vet. Clin. Food Anim. Pract."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.vetimm.2008.10.305","article-title":"Impact of oxidative stress on the health and immune function of dairy cattle","volume":"128","author":"Sordillo","year":"2009","journal-title":"Vet. Imm. Immunopathol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2017","DOI":"10.3168\/jds.S0022-0302(05)72878-2","article-title":"Influence of body condition score on relationships between metabolic status and oxidative stress in periparturient dairy cows","volume":"88","author":"Bernabucci","year":"2005","journal-title":"J. Dairy Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.tvjl.2004.02.001","article-title":"Oxidative status during late pregnancy and early lactation in dairy cows","volume":"169","author":"Castillo","year":"2005","journal-title":"Vet. J."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Halliwell, B., and Gutteridge, J.M. (2015). Free Radicals in Biology and Medicine, Oxford University Press.","DOI":"10.1093\/acprof:oso\/9780198717478.001.0001"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"4967","DOI":"10.3168\/jds.2015-10354","article-title":"Nutritional strategies to optimize dairy cattle immunity","volume":"99","author":"Sordillo","year":"2016","journal-title":"J. Dairy Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1016\/j.tvjl.2006.06.005","article-title":"Oxidants and antioxidants in disease: Oxidative stress in farm animals","volume":"173","author":"Lykkesfeldt","year":"2007","journal-title":"Vet. J."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1374","DOI":"10.1017\/S1751731113000396","article-title":"Oxidative stress index (OSi) as a new tool to assess redox status in dairy cattle during the transition period","volume":"7","author":"Abuelo","year":"2013","journal-title":"Animal"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.vetimm.2006.03.022","article-title":"Neutrophil function and energy status in Holstein cows with uterine health disorders","volume":"113","author":"Hammon","year":"2006","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.anireprosci.2013.11.007","article-title":"Interactions between negative energy balance, metabolic diseases, uterine health and immune response in transition dairy cows","volume":"144","author":"Esposito","year":"2014","journal-title":"Anim. Reprod. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2010","DOI":"10.3168\/jds.S0022-0302(05)72877-0","article-title":"Lymphocyte functions in overconditioned cows around parturition","volume":"88","author":"Lacetera","year":"2005","journal-title":"J. Dairy Sci."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Wnorowska, K., M\u0142ynek, K., Solarczyk, P., G\u0142owi\u0144ska, B., Tucki, K., and Puppel, K. (2025). Molecular Response of Simmental Cows to Negative Energy Balance: Regulation of Interleukin-6 and Plasminogen During Early Lactation. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms262311725"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1038\/cr.2010.178","article-title":"Crosstalk of reactive oxygen species and NF-\u03baB signaling","volume":"21","author":"Morgan","year":"2011","journal-title":"Cell Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.1089\/ars.2012.5149","article-title":"Reactive oxygen species in inflammation and tissue injury","volume":"20","author":"Mittal","year":"2014","journal-title":"Antioxid. Redox Signal."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1038\/nature09663","article-title":"A role for mitochondria in NLRP3 inflammasome activation","volume":"469","author":"Zhou","year":"2011","journal-title":"Nature"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1002\/emmm.201201771","article-title":"Of inflammasomes and pathogens\u2013sensing of microbes by the inflammasome","volume":"5","author":"Bauernfeind","year":"2013","journal-title":"EMBO Mol. Med."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1051\/vetres:2006007","article-title":"Innate immunity of the bovine mammary gland","volume":"37","author":"Rainard","year":"2006","journal-title":"Vet. Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.tvjl.2011.09.013","article-title":"The innate immune response of the bovine mammary gland to bacterial infection","volume":"192","author":"Wellnitz","year":"2012","journal-title":"Vet. J."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1023\/A:1020347818725","article-title":"Mammary gland immunity and mastitis susceptibility","volume":"7","author":"Sordillo","year":"2002","journal-title":"J. Mammary Gland Biol. Neoplasia"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"429","DOI":"10.5713\/ajas.2011.10233","article-title":"Relationship of somatic cell count and mastitis: An overview","volume":"24","author":"Sharma","year":"2011","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.cyto.2007.04.006","article-title":"Differential cytokine and chemokine responses of bovine mammary epithelial cells to Staphylococcus aureus and Escherichia coli","volume":"38","author":"Lahouassa","year":"2007","journal-title":"Cytokine"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Puppel, K., Sl\u00f3sarz, J., Solarczyk, P., Grodkowski, G., Kostusiak, P., Kali\u0144ska, A., Balcerak, M., Kunowska-Sl\u00f3sarz, M., and Go\u0142\u0119biewski, M. (2024). Assessing the usefulness of interleukin-8 as a biomarker of inflammation and metabolic dysregulation in dairy cows. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms252011129"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"10","DOI":"10.2527\/jas.2008-1187","article-title":"Pathogen-dependent induction of cytokines and other soluble inflammatory mediators during intramammary infection of dairy cows","volume":"87","author":"Bannerman","year":"2009","journal-title":"J. Anim. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Puppel, K., Staniszewska, P., Go\u0142\u0119biewski, M., Sl\u00f3sarz, J., Grodkowski, G., Solarczyk, P., Kunowska-Sl\u00f3sarz, M., Kostusiak, P., Kuczy\u0144ska, B., and Przysucha, T. (2021). Using the relationship between concentrations of selected whey proteins and BHBA to characterize the metabolism of dairy cows in early lactation. Animals, 11.","DOI":"10.3390\/ani11082298"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1641","DOI":"10.3168\/jds.S0022-0302(04)73319-6","article-title":"Nomenclature of the proteins of cows\u2019 milk\u2014Sixth revision","volume":"87","author":"Farrell","year":"2004","journal-title":"J. Dairy Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1146\/annurev.nutr.23.011702.073408","article-title":"Nutritional regulation of milk fat synthesis","volume":"23","author":"Bauman","year":"2003","journal-title":"Annu. Rev. Nutr."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1051\/animres:2000117","article-title":"Ruminant milk fat plasticity: Nutritional control of saturated, polyunsaturated, trans and conjugated fatty acids","volume":"49","author":"Chilliard","year":"2000","journal-title":"Ann. Zootech."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1007\/s11745-004-1348-6","article-title":"Modifying milk fat composition of dairy cows to enhance fatty acids beneficial to human health","volume":"39","author":"Lock","year":"2004","journal-title":"Lipids"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Puppel, K., Go\u0142\u0119biewski, M., Solarczyk, P., Grodkowski, G., Sl\u00f3sarz, J., Kunowska-Sl\u00f3sarz, M., Balcerak, M., Przysucha, T., Kali\u0144ska, A., and Kuczy\u0144ska, B. (2019). The relationship between plasma \u03b2-hydroxybutyric acid and conjugated linoleic acid in milk as a biomarker for early diagnosis of ketosis in postpartum Polish Holstein-Friesian cows. BMC Vet. Res., 15.","DOI":"10.1186\/s12917-019-2131-2"},{"key":"ref_56","first-page":"387","article-title":"Oleic acid as a biomarker for early diagnosis of elevated blood levels of non-esterified fatty acids and \u03b2-hydroxybutyric acid in the early stages of lactation in high-yielding Polish Holstein cows","volume":"35","author":"Puppel","year":"2017","journal-title":"Anim. Sci. Pap. Rep."},{"key":"ref_57","first-page":"92","article-title":"Multivariate analysis in metabolomics","volume":"1","author":"Worley","year":"2013","journal-title":"Curr. Metabolom."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1093\/nar\/gkae253","article-title":"MetaboAnalyst 6.0: Towards a unified platform for metabolomics data processing, analysis and interpretation","volume":"52","author":"Pang","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1038\/nrm.2016.25","article-title":"Metabolomics: Beyond biomarkers and towards mechanisms","volume":"17","author":"Johnson","year":"2016","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Fiore, F., Fiore, E., Contiero, B., and Lisuzzo, A. (2025). Ultrasound back-fat thickness association with risk of metabolic disease of dairy cows in early lactation. Animals, 15.","DOI":"10.3390\/ani15060883"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"8122","DOI":"10.3168\/jds.2023-23373","article-title":"Inflammatory tone in liver and adipose tissue in dairy cows experiencing a healthy transition from late pregnancy to early lactation","volume":"106","author":"McGuckin","year":"2023","journal-title":"J. Dairy Sci."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"68","DOI":"10.3168\/jds.S0022-0302(89)79081-0","article-title":"A body condition scoring chart for Holstein dairy cows","volume":"72","author":"Edmonson","year":"1989","journal-title":"J. Dairy Sci."},{"key":"ref_63","unstructured":"(2000). Animal and Vegetable Fats and Oils-Preparation of Methyl Esters of Fatty Acids (ISO 5509:2000) (Standard No. EN ISO 5509)."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"2179","DOI":"10.1002\/jsfa.7015","article-title":"The etiology of oxidative stress in the various species of animals, a review","volume":"95","author":"Puppel","year":"2015","journal-title":"J. Sci. 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