{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T05:20:31Z","timestamp":1787462431204,"version":"build-2736575974"},"reference-count":46,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Guangxi Natural Science Foundation","award":["2025GXNSFAA069329"],"award-info":[{"award-number":["2025GXNSFAA069329"]}]},{"name":"Guangxi Natural Science Foundation","award":["2023GXNSFBA026292"],"award-info":[{"award-number":["2023GXNSFBA026292"]}]},{"name":"the Open Project of Guangxi Key Laboratory of Biology for Mango","award":["GKLBMO2305"],"award-info":[{"award-number":["GKLBMO2305"]}]},{"name":"Guangxi Minzu University Research Funding Project","award":["2022KJQD18"],"award-info":[{"award-number":["2022KJQD18"]}]},{"award":["2022KJQD18"],"award-info":[{"award-number":["2022KJQD18"]}],"id":[{"id":"https:\/\/ror.org\/0495efn48","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Funds of Guangxi Academy of Agricultural Sciences","award":["Guinongke2025YP132"],"award-info":[{"award-number":["Guinongke2025YP132"]}]},{"name":"Funds of Guangxi Academy of Agricultural Sciences","award":["Guinongke2026YT001"],"award-info":[{"award-number":["Guinongke2026YT001"]}]},{"name":"Funds of Guangxi Academy of Agricultural Sciences","award":["Guinongke2026YP056"],"award-info":[{"award-number":["Guinongke2026YP056"]}]},{"award":["Guinongke2025YP132"],"award-info":[{"award-number":["Guinongke2025YP132"]}],"id":[{"id":"https:\/\/ror.org\/020rkr389","id-type":"ROR","asserted-by":"publisher"}]},{"award":["Guinongke2026YT001"],"award-info":[{"award-number":["Guinongke2026YT001"]}],"id":[{"id":"https:\/\/ror.org\/020rkr389","id-type":"ROR","asserted-by":"publisher"}]},{"award":["Guinongke2026YP056"],"award-info":[{"award-number":["Guinongke2026YP056"]}],"id":[{"id":"https:\/\/ror.org\/020rkr389","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Modern Agricultural Technology Industrial System Program","award":["nycytxgxcxtd-2021-06-01"],"award-info":[{"award-number":["nycytxgxcxtd-2021-06-01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Mango fruits are popular for their flavor. Sugar, a key component of fruit nutrition and flavor, determines fruit quality via its composition and content. While many studies explore molecular regulatory pathways of sugar metabolism in various fruits, large-scale physiological screening of related enzymes via advanced mathematical methods is largely neglected, and the physiological mechanism of sugar accumulation in mango remains unclear. This study analyzed glucose, fructose, sucrose and starch contents, and the activities of 11 sugar metabolism-related enzymes (adenosine diphosphate glucose pyrophosphorylase (AGP); sucrose synthase (SS); sucrose phosphate synthase (SPS); protein kinase (PK); starch debranching enzyme (DBE); phosphoglucomutase (PGM); \u03b1-amylase; \u03b2-amylase; isoamylase (ISA); sucrose invertase (INV); acid invertase (AI)) from fruit growth to post-ripening stages of mango cultivar \u2018Renong No.1\u2019, determined the correlations among soluble sugar, starch and enzyme activities, and constructed mathematical models of inter-group relationships using R language. The results showed that glucose, fructose and starch accumulated during fruit growth, while sucrose accumulated during post-ripening. Fructose content was significantly positively correlated with AGP, \u03b1-amylase, AI, \u03b2-amylase and SPS activities; so was glucose content and AGP activity; sucrose content and AGP, AI, PK, \u03b1-amylase, ISA, INV and SPS activities; and sweetness value and AGP, \u03b1-amylase, AI, SPS, SS and \u03b2-amylase activities. AI, AGP and \u03b1-amylase were identified as the key enzymes influencing sweetness value. Ultimately, a new visualizing and digitizing statistical approach for roles of eleven key enzymes in sweet value and soluble sugar component content during mango development was shown.<\/jats:p>","DOI":"10.3390\/ijms27167486","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T09:31:59Z","timestamp":1787304719000},"page":"7486","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Roles of Eleven Key Enzymes in Sweet Value and Soluble Sugar Component Content During Mango Development"],"prefix":"10.3390","volume":"27","author":[{"given":"Li","family":"Li","sequence":"first","affiliation":[{"name":"Guangxi Key Laboratory of Biology for Mango, College of Agriculture and Food Engineering, Baise University, Baise 533000, China"},{"name":"Guangxi Engineering Research Center of Green and Efficient Development for Mango Industry, Guangxi Subtropical Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zisong","family":"Wang","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Biology for Mango, College of Agriculture and Food Engineering, Baise University, Baise 533000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiming","family":"Li","sequence":"additional","affiliation":[{"name":"School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning 530008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2212-6402","authenticated-orcid":false,"given":"Xiang","family":"Li","sequence":"additional","affiliation":[{"name":"Guangxi Engineering Research Center of Green and Efficient Development for Mango Industry, Guangxi Subtropical Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaiyi","family":"Zou","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Biology for Mango, College of Agriculture and Food Engineering, Baise University, Baise 533000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofen","family":"Xie","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Biology for Mango, College of Agriculture and Food Engineering, Baise University, Baise 533000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenxing","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Biology for Mango, College of Agriculture and Food Engineering, Baise University, Baise 533000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanke","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Mathematics and Computer, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiehuan","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Mathematics and Computer, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guodi","family":"Huang","sequence":"additional","affiliation":[{"name":"Guangxi Engineering Research Center of Green and Efficient Development for Mango Industry, Guangxi Subtropical Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7874-7142","authenticated-orcid":false,"given":"Caihua","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Biology for Mango, College of Agriculture and Food Engineering, Baise University, Baise 533000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,21]]},"reference":[{"key":"ref_1","unstructured":"FAO (2026, August 17). Available online: https:\/\/www.fao.org\/faostat\/zh\/#data\/QCL."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Liu, B.D., Xin, Q., Zhang, M., Chen, J.H., Lu, Q.C., Zhou, X.Q., Li, X.X., Zhang, W.L., Feng, W., and Pei, H.S. (2023). Research progress on mango post-harvest ripening physiology and the regulatory technologies. Foods, 12.","DOI":"10.3390\/foods12010173"},{"key":"ref_3","first-page":"56","article-title":"Protection, Utilization status quo and development path of mango germplasm resources in Guangxi","volume":"37","author":"Huang","year":"2024","journal-title":"Agric. Res. Appl."},{"key":"ref_4","first-page":"259","article-title":"A Study on the relationships between acid invertase, sucrose synthase and sucrose metabolism in \u2018Red Fuji\u2019apple fruit","volume":"28","author":"Wang","year":"2001","journal-title":"Acta Hortic. Sin."},{"key":"ref_5","unstructured":"Zhang, L., and Chen, K.S. (2006). Molecular Physiology of Fruit Quality Development and Regulation, China Agricultural Press."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/0304-4238(94)00731-T","article-title":"Growth inhibition of persimmon fruit caused by calyx lobe removal and possible involvement of endogenous hormones","volume":"61","author":"Yonemori","year":"1995","journal-title":"Sci. Hortic."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1023\/A:1002912325702","article-title":"Estimation of the proportion of offspring having soluble solids content in fruit exceeding a given critical Value in Japanese persimmon","volume":"93","author":"Yamada","year":"1997","journal-title":"Euphytica"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"112787","DOI":"10.1016\/j.scienta.2023.112787","article-title":"Combined metabolome and transcriptome analyses of quality components and related molecular regulatory mechanisms during the ripening of Huangjin Peach","volume":"327","author":"Feng","year":"2024","journal-title":"Sci. Hortic."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"fyac074","DOI":"10.1093\/fqsafe\/fyac074","article-title":"Effects of 1-methylcyclopropene treatment on the quality of red \u2018Fuji\u2019 apples fruit during short-term storage","volume":"7","author":"Peng","year":"2023","journal-title":"Food Qual. Saf."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.21273\/HORTSCI.34.6.1024","article-title":"Modification of carbohydrate content in developing tomato fruit","volume":"34","author":"Schaffer","year":"1999","journal-title":"HortScience"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"149","DOI":"10.17660\/ActaHortic.1989.239.20","article-title":"The grape berry as a sink","volume":"239","author":"Coombe","year":"1989","journal-title":"Acta Hortic."},{"key":"ref_12","first-page":"519","article-title":"Progress on the research of the molecular regulation of sugar content and composition in fruit","volume":"22","author":"Qin","year":"2005","journal-title":"J. Fruit Sci."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Zhang, A., Yang, X., Liu, D., Luo, Z., Zhang, Z., Huo, J., Han, D., Zhang, Y., and Zhang, L. (2025). Genome-wide characterization of kiwifruit invertase gene family reveals roles of AcCWINV4 in sugar accumulation and cold tolerance. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms262010089"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Chen, C., Chen, H., Yang, W., Li, J., Tang, W., and Gong, R. (2022). Transcriptomic and metabolomic analysis of quality changes during sweet cherry fruit development and mining of related genes. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23137402"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Gou, N., Chen, C., Huang, M., Zhang, Y., Bai, H., Li, H., Wang, L., and Wuyun, T. (2023). Transcriptome and metabolome analyses reveal sugar and acid accumulation during apricot fruit development. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms242316992"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"142821","DOI":"10.1016\/j.ijbiomac.2025.142821","article-title":"Discovery and interaction of four key biosynthetic enzymes under co-regulation for dopamine biosynthesis with marine Meyerozyma guilliermondii GXDK6 and Bacillus aryabhattai NM1-A2","volume":"309","author":"Sun","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1111\/j.1745-4514.2008.00175.x","article-title":"Starch mobilization and sucrose accumulation in the pulp of Keitt mangoes during postharvest ripening","volume":"32","author":"Nascimento","year":"2008","journal-title":"J. Food Biochem."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Maldonado-Celis, M.E., Yahia, E.M., Bedoya, R., Land\u00e1zuri, P., Loango, N., Aguill\u00f3n, J., Restrepo, B., and Guerrero Ospina, J.C. (2019). Chemical composition of mango (Mangifera indica L.) fruit: Nutritional and phytochemical compounds. Front. Plant Sci., 10.","DOI":"10.3389\/fpls.2019.01073"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4505","DOI":"10.1016\/j.ygeno.2020.07.038","article-title":"Transcriptional mechanism of differential sugar accumulation in pulp of two contrasting mango (Mangifera indica L.) cultivars","volume":"112","author":"Li","year":"2020","journal-title":"Genomics"},{"key":"ref_20","unstructured":"Seymour, G.B., Taylor, J.E., and Tucker, G.A. (1996). Biochemistry of Fruit Ripening, Chapman & Hall."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1002\/jsfa.2740360707","article-title":"Analysis of sugars and organic acids in ripening mango fruits (Mangifera indica L. var Keitt) by high performance liquid chromatography","volume":"36","author":"Medlicott","year":"1985","journal-title":"J. Sci. Food Agric."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"7410","DOI":"10.1021\/jf800467v","article-title":"Mango starch degradation. I. a microscopic view of the granule during ripening","volume":"56","author":"Silva","year":"2008","journal-title":"J. Agric. Food Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1104\/pp.98.3.1163","article-title":"Sucrose synthase in wild tomato, Lycopersicon chemieleleuskii, and tomato fruit sink strength","volume":"98","author":"Sun","year":"1992","journal-title":"Plant Physiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"112832","DOI":"10.1016\/j.scienta.2023.112832","article-title":"Exploring the role of nitrogen and potassium in photosynthesis implications for sugar: Accumulation and translocation in horticultural crops","volume":"327","author":"Shah","year":"2024","journal-title":"Sci. Hortic."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.1104\/pp.90.4.1394","article-title":"Sucrose-metabolizing enzymes in transport tissue and adjacent sink structures in developing citrus fruit","volume":"90","author":"Lowell","year":"1989","journal-title":"Plant Physiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1093\/jexbot\/53.366.61","article-title":"Analysis of sucrose synthase genes in citrus suggests different roles and phylogenetic relationships","volume":"53","author":"Komatsu","year":"2002","journal-title":"J. Exp. Bot."},{"key":"ref_27","unstructured":"Mattoo, A.K., and Modi, V.V. (1970). Biochemical aspects of ripening and chilling injury in mango fruit. Proceedings of the Tropical Product Institute Conference, Tropical Products Institute. Available online: https:\/\/www.mendeley.com\/catalogue\/7939618f-bea2-3059-b2ed-ad618d1f577e\/."},{"key":"ref_28","first-page":"972","article-title":"Sucrose metabolism in Irwin mango (Mangifera indica L.) during maturation","volume":"21","author":"Wei","year":"2008","journal-title":"Southwest China J. Agric. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1590\/S1677-04202009000400001","article-title":"Expression analysis of a set of genes related to the ripening of bananas and mangoes","volume":"21","author":"Godoy","year":"2009","journal-title":"Braz. J. Plant Physiol."},{"key":"ref_30","first-page":"24","article-title":"The relationship between sugar accumulation and related enzyme activities during the development process of \u2018KRS\u2019 mango fruits","volume":"44","author":"Wu","year":"2016","journal-title":"J. Anhui Agric. Sci."},{"key":"ref_31","first-page":"735","article-title":"Sucrose metabolism in fruits of mango cultivar Yuexi No. 1 (Mangifera indica L.) at the mature stage","volume":"30","author":"Wei","year":"2009","journal-title":"Chin. J. Trop. Crops"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"109235","DOI":"10.1016\/j.plaphy.2024.109235","article-title":"Characterization of ATP-dependent phosphofructokinase genes during ripening and their modulation by phytohormones during postharvest storage of citrus fruits (Citrus reticulata Blanco.)","volume":"217","author":"Ochiki","year":"2024","journal-title":"Plant Physiol. Biochem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"127144","DOI":"10.1016\/j.ijbiomac.2023.127144","article-title":"MaNAC19\u2013MaXB3 regulatory module mediates sucrose synthesis in banana fruit during ripening","volume":"253","author":"Wei","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Wang, P., Feng, X., Jiang, J., Yan, P., Li, Z., Luo, W., Chen, Y., and Ye, W. (2023). Transcriptome analysis reveals fruit quality formation in Actinidia eriantha Benth. Plants, 12.","DOI":"10.3390\/plants12244079"},{"key":"ref_35","first-page":"3453","article-title":"Difference in starch accumulation and characterization of sugar metabolism during fruit development of kiwi fruit","volume":"47","author":"Zhang","year":"2014","journal-title":"Sci. Agric. Sin."},{"key":"ref_36","first-page":"1","article-title":"Sugar accumulation and related enzyme activities in the litchi fruit of \u2018Nuomici\u2019 and \u2018Feizixiao\u2019","volume":"30","author":"Wang","year":"2003","journal-title":"Acta Hortic. Sin."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1093\/pcp\/41.4.408","article-title":"Purification and characterization of two sucrose synthase isoforms from Japanese pear fruit","volume":"41","author":"Tanase","year":"2000","journal-title":"Plant Cell Physiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.tplants.2017.11.001","article-title":"Evolution of sucrose metabolism: The dichotomy of invertases and beyond","volume":"23","author":"Wan","year":"2018","journal-title":"Trends Plant Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1104\/pp.120.1.257","article-title":"Two SNF1-related protein kinases from spinach leaf phosphorylate and inactivate 3-hydroxy-3-methylglutaryl-coenzyme a reductase, nitrate reductase, and sucrose phosphate synthase in vitro","volume":"120","author":"Sugden","year":"1999","journal-title":"Plant Physiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1046\/j.1365-313X.1998.00108.x","article-title":"Antisense expression of a sucrose nonfermenting-1-related protein kinase sequence in potato results in decreased expression of sucrose synthase in tubers and loss of sucrose-inducibility of sucrose synthase transcripts in leaves","volume":"14","author":"Purcell","year":"1998","journal-title":"Plant J."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.tplants.2006.11.005","article-title":"SNF1\/AMPK\/SnRK1 kinases, global regulators at the heart of energy control","volume":"12","author":"Polge","year":"2007","journal-title":"Trends Plant Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/S0092-8674(00)80107-5","article-title":"From glycogen to amylopectin: A model for the biogenesis of the plant starch granule","volume":"86","author":"Ball","year":"1996","journal-title":"Cell"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1699","DOI":"10.1105\/tpc.10.10.1699","article-title":"A mutant of Arabidopsis lacking a chloroplastic isoamylase accumulates both starch and phytoglycogen","volume":"10","author":"Zeeman","year":"1998","journal-title":"Plant Cell"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1105\/tpc.006635","article-title":"Three isoforms of isoamylase contribute different catalytic properties for the debranching of potato glucans","volume":"15","author":"Hussain","year":"2003","journal-title":"Plant Cell"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"125219","DOI":"10.1016\/j.carbpol.2026.125219","article-title":"Low-molecular-weight ferulic oligosaccharides derived from rice bran enhance the inhibitory effect on rice starch digestion by attenuating self-aggregation","volume":"381","author":"Chang","year":"2026","journal-title":"Carbohydr. Polym."},{"key":"ref_46","first-page":"4229","article-title":"Characteristics of components and contents of soluble sugars in pear fruits from different species","volume":"43","author":"Yao","year":"2010","journal-title":"Sci. Agric. Sin."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/16\/7486\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T04:22:08Z","timestamp":1787458928000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/16\/7486"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,21]]},"references-count":46,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["ijms27167486"],"URL":"https:\/\/doi.org\/10.3390\/ijms27167486","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,21]]}}}