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J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Sec61\u03b2 encodes one of the subunits of the Sec61 translocon, which is essential for translocation of the nascent polypeptides to the endoplasmic reticulum (ER). Knockdown of Sec61\u03b2 in the testis leads to male sterility in Drosophila melanogaster. Although Sec61\u03b2 is expressed in both testes and ovaries, its role in female reproduction remains unclear. In this study, we found that knockdown of Sec61\u03b2 in either germ cells or somatic cells of ovaries causes complete female sterility, with a small size of ovaries from larval to adult stages, significant reduction in germ cell numbers beginning in the late third-instar larval stage and a complete absence of germ cells in pupal and adult ovaries. Overexpression of Sec61\u03b2 efficiently rescues these defects. Furthermore, we observed that Sec61\u03b2 knockdown in the germline triggers the unfolded-protein response (UPR), which not only non-autonomously activates JAK\/STAT signaling in adjacent somatic cells to inhibit Bam expression but also autonomously impedes the G1\/S phase progression of germ cells. These effects collectively impact cell proliferation and differentiation. Our results suggest that Sec61\u03b2 is essential for germ cell maintenance via UPR-mediated cell-autonomous proliferation control and non-autonomous JAK\/STAT signaling in Drosophila ovaries.<\/jats:p>","DOI":"10.3390\/ijms27177640","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T10:59:23Z","timestamp":1787741963000},"page":"7640","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sec61\u03b2 Deficiency Disrupts Drosophila Oogenesis Through UPR-Mediated Defects in Germ Cell Proliferation and Differentiation"],"prefix":"10.3390","volume":"27","author":[{"given":"Zhi-Xian","family":"Cao","sequence":"first","affiliation":[{"name":"Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Min","family":"Xiao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Ming","family":"Ji","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuan-Xiang","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-Qiu","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1823-373X","authenticated-orcid":false,"given":"Yu-Feng","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-1-0716-2970-3_1","article-title":"The Vast Utility of Drosophila Oogenesis","volume":"Volume 2626","author":"Giedt","year":"2023","journal-title":"Drosophila Oogenesis"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.cub.2023.12.033","article-title":"Juvenile Hormones Direct Primordial Germ Cell Migration to the Embryonic Gonad","volume":"34","author":"Barton","year":"2024","journal-title":"Curr. 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