{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T05:19:34Z","timestamp":1788326374738,"version":"build-2803163510"},"reference-count":53,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T00:00:00Z","timestamp":1787961600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004505","name":"University of Catania","doi-asserted-by":"crossref","award":["Progetti di ricerca collaborativa\u201d PIACERI 2024\u20132026 to C.F."],"award-info":[{"award-number":["Progetti di ricerca collaborativa\u201d PIACERI 2024\u20132026 to C.F."]}],"id":[{"id":"10.13039\/501100004505","id-type":"DOI","asserted-by":"crossref"}]},{"award":["Progetti di ricerca collaborativa\u201d PIACERI 2024\u20132026 to C.F."],"award-info":[{"award-number":["Progetti di ricerca collaborativa\u201d PIACERI 2024\u20132026 to C.F."]}],"id":[{"id":"https:\/\/ror.org\/03a64bh57","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Sergentomyia minuta is a widespread Mediterranean sand fly traditionally regarded as herpetophilic and of limited relevance for human leishmaniasis. Occasional detection of mammalian Leishmania DNA in Sergentomyia species has challenged this assumption. This study aimed to investigate the abundance, host-feeding behaviour, and molecular detection of Leishmania infantum DNA in S. minuta in peri-urban areas of southern Italy. Sand flies were collected using sticky traps at three sites in the Catania area (Italy). Unengorged females were screened for Leishmania spp. DNA using ITS1 and SSU rRNA markers. ITS1 PCR-RFLP was used exclusively to discriminate L. infantum-compatible from non-L. infantum molecular profiles. Engorged females were analysed for blood-meal origin using mitochondrial cytochrome b markers (CytB1 and CytB2) and a nuclear human-specific transthyretin (TTR) marker through PCR-RFLP and multiplex PCR. A total of 1361 S. minuta specimens were collected, with a strongly female-biased sex ratio. Leishmania spp. DNA was detected in a substantial proportion of pooled samples. ITS1 PCR-RFLP profiles revealed heterogeneous molecular patterns that consistently excluded L. infantum and were compatible with non-L. infantum trypanosomatid DNA. Blood-meal analyses suggested feeding on multiple vertebrate hosts, including occasional detection of human mitochondrial DNA, whereas the nuclear TTR marker was consistently negative, likely reflecting DNA degradation during blood digestion. S. minuta populations from southern Italy display high abundance, ecological plasticity, and a strong association with peri-urban habitats. Our findings refine, rather than overturn, the current understanding of the epidemiological role of S. minuta in Mediterranean ecosystems. Multilocus molecular screening revealed heterogeneous non-L. infantum trypanosomatid DNA patterns, while no evidence of Leishmania infantum was detected. Occasional human blood meals were detected, but the epidemiological relevance of S. minuta for human leishmaniasis remains unproven. These findings highlight the importance of integrated entomological and molecular approaches for careful interpretation of vector\u2013parasite\u2013host relationships Mediterranean ecosystems.<\/jats:p>","DOI":"10.3390\/ijms27177741","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T09:48:36Z","timestamp":1788256116000},"page":"7741","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Targeted Molecular Screening of Leishmania infantum in Sergentomyia minuta Populations from Southern Italy"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-2513-1349","authenticated-orcid":false,"given":"Desiree","family":"Brancato","sequence":"first","affiliation":[{"name":"Department of Biological, Geological and Environmental Sciences, University of Catania, 95124 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnese","family":"Messina","sequence":"additional","affiliation":[{"name":"Department of Biological, Geological and Environmental Sciences, University of Catania, 95124 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar","family":"Lisi","sequence":"additional","affiliation":[{"name":"Department of Biological, Geological and Environmental Sciences, University of Catania, 95124 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2704-0013","authenticated-orcid":false,"given":"Francesca","family":"Bruno","sequence":"additional","affiliation":[{"name":"Department of Biological, Geological and Environmental Sciences, University of Catania, 95124 Catania, Italy"},{"name":"Department of Medicine and Surgery, Kore University of Enna, 94100 Enna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elvira","family":"Coniglio","sequence":"additional","affiliation":[{"name":"Department of Biological, Geological and Environmental Sciences, University of Catania, 95124 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Vadal\u00e0","sequence":"additional","affiliation":[{"name":"Department of Biological, Geological and Environmental Sciences, University of Catania, 95124 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Maria","family":"Pappalardo","sequence":"additional","affiliation":[{"name":"Department of Biological, Geological and Environmental Sciences, University of Catania, 95124 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Venera","family":"Ferrito","sequence":"additional","affiliation":[{"name":"Department of Biological, Geological and Environmental Sciences, University of Catania, 95124 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7568-5823","authenticated-orcid":false,"given":"Salvatore","family":"Saccone","sequence":"additional","affiliation":[{"name":"Department of Biological, Geological and Environmental Sciences, University of Catania, 95124 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Concetta","family":"Federico","sequence":"additional","affiliation":[{"name":"Department of Biological, Geological and Environmental Sciences, University of Catania, 95124 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,29]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2024). Global Health Estimates 2021: Deaths by Cause, Age, Sex, by Country and by Region, 2000\u20132021, WHO. Available online: https:\/\/www.who.int\/data\/gho\/data\/themes\/mortality-and-global-health-estimates\/ghe-leading-causes-of-death?authuser=0&utm_source=chatgpt.com."},{"key":"ref_2","unstructured":"World Health Organization (2023). Leishmaniasis\u2014Fact Sheet, WHO. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/leishmaniasis."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Nemhauser, J.B. (2023). CDC Yellow Book 2024: Health Information for International Travel, Oxford University Press.","DOI":"10.1093\/oso\/9780197570944.001.0001"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"19505","DOI":"10.2807\/ese.15.10.19505-en","article-title":"Leishmaniasis Emergence in Europe","volume":"15","author":"Ready","year":"2010","journal-title":"Euro Surveill."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.21767\/1791-809X.1000519","article-title":"Leishmaniasis: A Review on Parasite, Vector and Reservoir Host","volume":"11","author":"Alemayehu","year":"2017","journal-title":"Health Sci. J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1038\/s41572-025-00663-w","article-title":"Leishmaniasis","volume":"11","author":"Pareyn","year":"2025","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mam.2016.11.012","article-title":"Leishmania Infections: Molecular Targets and Diagnosis","volume":"57","author":"Akhoundi","year":"2017","journal-title":"Mol. Asp. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1186\/s13071-024-06631-9","article-title":"Comparison of ELISA and IFAT for Leishmania infantum by European and Middle Eastern Diagnostic Laboratories","volume":"17","author":"Mahachi","year":"2024","journal-title":"Parasites Vectors"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e00395","DOI":"10.1016\/j.parepi.2024.e00395","article-title":"Molecular Detection of Leishmania DNA in Wild-Caught Sand Flies, Phlebotomus and Sergentomyia spp. in Northern Iran","volume":"27","author":"Nikookar","year":"2024","journal-title":"Parasite Epidemiol. Control"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1186\/s13071-024-06542-9","article-title":"Phlebotomine Sand Fly Survey, Blood Meal Source Identification, and Description of Sergentomyia imihra n. sp. in the Central Sahara of Algeria","volume":"17","author":"Benallal","year":"2024","journal-title":"Parasites Vectors"},{"key":"ref_11","first-page":"69","article-title":"Leishmaniasis Vectors in Italy","volume":"3","author":"Maroli","year":"1998","journal-title":"G. Ital. Med. Trop."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1186\/s13071-014-0560-0","article-title":"Persistence of Phlebotomine Leishmania Vectors in Urban Sites of Catania (Sicily, Italy)","volume":"7","author":"Lisi","year":"2014","journal-title":"Parasites Vectors"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Bruno, F., Vitale, F., La Russa, F., Reale, S., Sp\u00e4th, G.F., Oliveri, E., Gargano, V., Valenza, V., Facciponte, F., and Giardina, S. (2022). Retrospective Analysis of Leishmaniasis in Sicily (Italy) from 2013 to 2021: One-Health Impact and Future Control Strategies. Microorganisms, 10.","DOI":"10.3390\/microorganisms10091704"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"107704","DOI":"10.1016\/j.actatropica.2025.107704","article-title":"Detection and Isolation of Leishmania infantum and Leishmania tarentolae in Sand Flies from a Canine Leishmaniasis Endemic Area","volume":"268","author":"Ricci","year":"2025","journal-title":"Acta Trop."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"13297","DOI":"10.1038\/s41598-025-96296-w","article-title":"Species-Specific Variation in Predicted Distribution and Habitat Suitability of Phlebotomine Sand Flies in Italy under Different Climate Change Scenarios","volume":"15","author":"Bernardini","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.vetpar.2018.02.006","article-title":"Detection of Leishmania infantum DNA in Phlebotomine Sand Flies from an Area Where Canine Leishmaniosis Is Endemic in Southern Italy","volume":"253","author":"Latrofa","year":"2018","journal-title":"Vet. Parasitol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1111\/mve.12464","article-title":"Molecular Detection of Leishmania (Sauroleishmania) tarentolae in Human Blood and Leishmania (Leishmania) infantum in Sergentomyia minuta: Unexpected Host-Parasite Contacts","volume":"34","author":"Pombi","year":"2020","journal-title":"Med. Vet. Entomol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1186\/s13071-015-0787-4","article-title":"Molecular Detection of Leishmania DNA and Identification of Blood Meals in Wild Caught Phlebotomine Sand Flies (Diptera: Psychodidae) from Southern Portugal","volume":"8","author":"Maia","year":"2015","journal-title":"Parasites Vectors"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1186\/s13071-015-1269-4","article-title":"First Report of Naturally Infected Sergentomyia minuta with Leishmania major in Tunisia","volume":"8","author":"Jaouadi","year":"2015","journal-title":"Parasites Vectors"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1590\/S0074-02762013000400020","article-title":"The First Detection of Leishmania major in Naturally Infected Sergentomyia minuta in Portugal","volume":"108","author":"Campino","year":"2013","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.actatropica.2017.06.020","article-title":"First Molecular Detection of Leishmania infantum in Sergentomyia minuta (Diptera, Psychodidae) in Alentejo, Southern Portugal","volume":"174","author":"Pereira","year":"2017","journal-title":"Acta Trop."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1186\/s13071-023-05758-5","article-title":"Experimental Feeding of Sergentomyia minuta on Reptiles and Mammals: Comparison with Phlebotomus papatasi","volume":"16","author":"Ticha","year":"2023","journal-title":"Parasites Vectors"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Abbate, J.M., Maia, C., Pereira, A., Arfuso, F., Gaglio, G., Rizzo, M., Caracappa, G., Marino, G., Pollmeier, M., and Giannetto, S. (2020). Identification of Trypanosomatids and Blood Feeding Preferences of Phlebotomine Sand Fly Species Common in Sicily, Southern Italy. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0229536"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"106936","DOI":"10.1016\/j.actatropica.2023.106936","article-title":"Molecular Detection of Leishmania (Sauroleishmania) adleri (Trypanosomatida: Trypanosomatidae) in Sergentomyia sp. Sand Flies (Diptera: Psychodidae) in Mali and Niger","volume":"243","author":"Balczun","year":"2023","journal-title":"Acta Trop."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1051\/parasite\/2016062","article-title":"Can Sergentomyia (Diptera, Psychodidae) Play a Role in the Transmission of Mammal-Infecting Leishmania?","volume":"23","author":"Maia","year":"2016","journal-title":"Parasite"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Bennai, K., Tahir, D., Lafri, I., Bendjaballah-Laliam, A., Bitam, I., and Parola, P. (2018). Molecular Detection of Leishmania infantum DNA and Host Blood Meal Identification in Phlebotomus in a Hypoendemic Focus of Human Leishmaniasis in Northern Algeria. PLoS Negl. Trop. Dis., 12.","DOI":"10.1371\/journal.pntd.0006513"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1393","DOI":"10.1111\/tbed.13464","article-title":"Leishmania sp. Detection and Blood-Feeding Behaviour of Sergentomyia minuta Collected in the Human Leishmaniasis Focus of Southwestern Madrid, Spain (2012\u20132017)","volume":"67","author":"Molina","year":"2020","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1111\/j.1365-2915.2012.01034.x","article-title":"Phlebotomine Sandflies and the Spreading of Leishmaniases and Other Diseases of Public Health Concern","volume":"27","author":"Maroli","year":"2013","journal-title":"Med. Vet. Entomol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2423","DOI":"10.1111\/tbed.13580","article-title":"Leishmaniasis Due to Leishmania infantum: Integration of Human, Animal and Environmental Data through a One Health Approach","volume":"67","author":"Sampedro","year":"2020","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1016\/j.trstmh.2008.01.011","article-title":"Asymptomatic Leishmania infantum\/chagasi Infection in Blood Donors of Western Sicily","volume":"102","author":"Scarlata","year":"2008","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1878","DOI":"10.1017\/S003118201100148X","article-title":"Predicting the Distribution of Canine Leishmaniasis in Western Europe Based on Environmental Variables","volume":"138","author":"Franco","year":"2011","journal-title":"Parasitology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1186\/s13071-022-05388-3","article-title":"Concurrent Molecular Characterization of Sand Flies and Leishmania Parasites by Amplicon-Based next-Generation Sequencing","volume":"15","author":"Nasereddin","year":"2022","journal-title":"Parasites Vectors"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.exppara.2006.04.001","article-title":"Analysis of Mosquito Bloodmeals Using RFLP Markers","volume":"114","author":"Oshaghi","year":"2006","journal-title":"Exp. Parasitol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S0001-706X(03)00190-6","article-title":"Host Preferences of Phlebotomine Sand Flies at a Hypoendemic Focus of Canine Leishmaniasis in Central Italy","volume":"88","author":"Bongiorno","year":"2003","journal-title":"Acta Trop."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1111\/j.1948-7134.2013.12053.x","article-title":"Comparative Field Study to Evaluate the Performance of Three Different Traps for Collecting Sand Flies in Northeastern Italy","volume":"38","author":"Signorini","year":"2013","journal-title":"J. Vector Ecol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1186\/s13071-020-04249-1","article-title":"Understanding Sand Fly Sampling Methods: Sticky Traps Are Attraction-Based and Not Interceptive Sampling Tools of Phlebotomus orientalis","volume":"13","author":"Elnaiem","year":"2020","journal-title":"Parasites Vectors"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.actatropica.2010.08.013","article-title":"Phlebotomine Sand Fly Population Dynamics in a Leishmaniasis Endemic Peri-Urban Area in Southern Italy","volume":"116","author":"Tarallo","year":"2010","journal-title":"Acta Trop."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.vetpar.2011.08.031","article-title":"Towards a Rapid Molecular Identification of the Common Phlebotomine Sand Flies in the Mediterranean Region","volume":"184","author":"Latrofa","year":"2012","journal-title":"Vet. Parasitol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/S0732-8893(03)00093-2","article-title":"PCR Diagnosis and Characterization of Leishmania in Local and Imported Clinical Samples","volume":"47","author":"Nasereddin","year":"2003","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"105570","DOI":"10.1016\/j.rvsc.2025.105570","article-title":"Development of an RFLP-PCR Assay for Discrimination of Three Prevalent Old World Leishmania Species in Canine and Human Samples","volume":"186","author":"Fazeli","year":"2025","journal-title":"Res. Vet. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"62","DOI":"10.4103\/1995-7645.275414","article-title":"A PCR and RFLP-Based Molecular Diagnostic Algorithm for Visceral Leishmaniasis","volume":"13","author":"Lindoso","year":"2020","journal-title":"Asian Pac. J. Trop. Med."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Usmael, U.A., Tesema, N.B., Girma, S., Kendie, D.A., and Abas, M.K. (2022). Detection of Leishmania donovani Using ITS1-RFLP from Positive and Negative Smear Samples among Clinically Reported Patients Visiting University of Gondar Comprehensive Specialized Hospital. BMC Infect. Dis., 22.","DOI":"10.1186\/s12879-022-07930-1"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Brancato, D., Treccarichi, S., Bruno, F., Coniglio, E., Vinci, M., Saccone, S., Cal\u00ec, F., and Federico, C. (2025). NGS Approaches in Clinical Diagnostics: From Workflow to Disease-Specific Applications. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26199597"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1186\/s13071-023-05884-0","article-title":"Efficiency of Mitochondrial Genes and Nuclear Alu Elements in Detecting Human DNA in Blood Meals of Anopheles stephensi Mosquitoes: A Time-Course Study","volume":"16","author":"Talebzadeh","year":"2023","journal-title":"Parasites Vectors"},{"key":"ref_45","first-page":"8","article-title":"Blood Meal Identification in Field-Captured Sand Flies: Comparison of PCR-RFLP and ELISA Assays","volume":"3","author":"Oshaghi","year":"2009","journal-title":"Iran. J. Arthropod-Borne Dis."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.actatropica.2015.08.020","article-title":"Identification of Blood Meals in Field Captured Sand Flies by a PCR-RFLP Approach Based on Cytochrome b Gene","volume":"152","author":"Molina","year":"2015","journal-title":"Acta Trop."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1186\/s13071-014-0479-5","article-title":"Morphological Keys for the Identification of Italian Phlebotomine Sand Flies (Diptera: Psychodidae: Phlebotominae)","volume":"7","author":"Tarallo","year":"2014","journal-title":"Parasites Vectors"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/S0035-9203(00)90093-2","article-title":"Genetic Heterogeneity of Ribosomal Internal Transcribed Spacer in Clinical Samples of Leishmania donovani Spotted on Filter Paper as Revealed by Single-Strand Conformation Polymorphisms and Sequencing","volume":"94","author":"Osman","year":"2000","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_49","first-page":"419","article-title":"Development and Application of the Polymerase Chain Reaction for the Detection and Identification of Leishmania Parasites in Clinical Material","volume":"70","author":"Meredith","year":"1993","journal-title":"Arch. L\u2019institut Pasteur Tunis"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1111\/j.1755-0998.2010.02920.x","article-title":"Taxon-Specific PCR for DNA Barcoding Arthropod Prey in Bat Faeces","volume":"11","author":"Zeale","year":"2011","journal-title":"Mol. Ecol. Resour."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1007\/s00439-017-1810-y","article-title":"Somatic Mosaicism with Reversion to Normality of a Mutated Transthyretin Allele Related to a Familial Amyloidotic Polyneuropathy","volume":"136","author":"Federico","year":"2017","journal-title":"Hum. Genet."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"336","DOI":"10.4269\/ajtmh.2005.73.336","article-title":"Identification of Mammalian Blood Meals in Mosquitoes by a Multiplexed Polymerase Chain Reaction Targeting Cytochrome B","volume":"73","author":"Kent","year":"2005","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Dugo, K., Bruno, F., Sturiale, V., Brancato, D., Saccone, S., and Federico, C. (2022). Hereditary Transthyretin-Related Amyloidosis: Genetic Heterogeneity and Early Personalized Gene Therapy. Biomedicines, 10.","DOI":"10.3390\/biomedicines10102394"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/17\/7741\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T04:28:44Z","timestamp":1788323324000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/17\/7741"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,29]]},"references-count":53,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["ijms27177741"],"URL":"https:\/\/doi.org\/10.3390\/ijms27177741","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,29]]}}}