{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T13:13:44Z","timestamp":1788527624047,"version":"build-2803163510"},"reference-count":127,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Calcium signaling plays a central role in plant adaptation to abiotic stresses and is primarily mediated by calmodulin and its associated transcription factors. Calmodulin-binding transcription activators (CAMTAs) regulate stress-responsive gene expression, but their characteristics and functions remain largely unexplored in Aegilops tauschii Coss., the D-genome progenitor of bread wheat. In this study, a genome-wide identification and characterization of the CAMTA gene family was performed, followed by phylogenetic, structural, conserved domain, promoter cis-element, and expression analyses under drought stress. Five AetCAMTA genes were identified and classified into three phylogenetic groups. All proteins contained conserved CG-1 DNA-binding, ankyrin repeat (ANK), and IQ calmodulin-binding domains and exhibited similar exon\u2013intron organization. Promoter analysis revealed abundant hormone- and stress-responsive cis-elements, particularly abscisic acid-responsive element (ABRE) and drought-responsive MYB-binding site (MBS) motifs, suggesting their involvement in drought-responsive signaling. Quantitative RT-PCR showed genotype- and stress-dependent expression patterns, with the drought-tolerant ecotype (TN-01-1747) exhibiting higher expression of AetCAMTA1, AetCAMTA2, and AetCAMTA3 than the drought-sensitive ecotype (TN-01-1559) under moderate drought stress. These findings provide new insights into the evolutionary and functional characteristics of AetCAMTA genes and identify promising candidates for improving drought tolerance in wheat through molecular breeding and biotechnological approaches.<\/jats:p>","DOI":"10.3390\/ijms27177790","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T09:48:36Z","timestamp":1788256116000},"page":"7790","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome-Wide Characterization of Calmodulin-Binding Transcription Activators Genes in Aegilops tauschii"],"prefix":"10.3390","volume":"27","author":[{"given":"Elnaz","family":"Nozari","sequence":"first","affiliation":[{"name":"Agricultural Biotechnology Program, Department of Plant Production and Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil 56199-11367, Iran"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"validation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"conceptualization","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1613-4455","authenticated-orcid":false,"given":"Rasool","family":"Asghari-Zakaria","sequence":"additional","affiliation":[{"name":"Agricultural Biotechnology Program, Department of Plant Production and Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil 56199-11367, Iran"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"given":"Nasser","family":"Zare","sequence":"additional","affiliation":[{"name":"Agricultural Biotechnology Program, Department of Plant Production and Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil 56199-11367, Iran"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"supervision","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"}]},{"given":"Parisa","family":"Sheikhzadeh","sequence":"additional","affiliation":[{"name":"Agricultural Biotechnology Program, Department of Plant Production and Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil 56199-11367, Iran"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0428-822X","authenticated-orcid":false,"given":"Mehran","family":"Nozari-Asbemarz","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences, Bernal Institute, University of Limerick, V94 T9PX Limerick, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"validation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.scienta.2018.09.022","article-title":"Genome-wide identification and expression analysis of calmodulin-binding transcription activator genes in banana under drought stress","volume":"244","author":"Meer","year":"2019","journal-title":"Sci. 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