{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T05:16:48Z","timestamp":1788499008340,"version":"build-2803163510"},"reference-count":48,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Programs for Science and Technology Development of Henan province","award":["262102111156"],"award-info":[{"award-number":["262102111156"]}]},{"name":"The 14th Five-Year National key Research and development Plan","award":["2023YFD2201202"],"award-info":[{"award-number":["2023YFD2201202"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Light critically limits rattan seedling establishment. To elucidate adaptive mechanisms of Calamus viminalis under varied light, we conducted a 360-day pot experiment with four shading levels (0%, 20%, 50%, 75%), integrating morphological, physiological, antioxidant, and RNA-seq analyses. Moderate shading (20%) maximized seedling height (46.97 cm) and ground diameter (12.46 mm), increased net photosynthetic rate by 57.92% versus full light, and raised soluble protein\/sugar while minimizing MDA and proline\u2014indicating the lowest oxidative damage. Severe shading (75%) suppressed carbon fixation, reduced antioxidant enzymes, aggravated lipid peroxidation, and drastically lowered survival. Transcriptomics revealed intensity-dependent reprogramming: moderate shading activated phenylpropanoid\/flavonoid defense pathways; severe shading upregulated photosynthesis\/ribosomal genes but constrained overall carbon flux. Four core pathways (photosynthesis, hormone signaling, phenylpropanoid metabolism, carbon metabolism) coordinated shade responses\u2014BR signaling upregulated (shade avoidance) while ABA downregulated under low light; light deprivation globally downregulated LHCB1 and rbcL, impairing capture\/assimilation. We identify 20% shading as optimal for C. viminalis cultivation and reveal multi-level phenotype\u2013physiology\u2013molecule mechanisms, providing a theoretical basis for Calamus viminalis seedling nursery management under controlled nursery conditions.<\/jats:p>","DOI":"10.3390\/ijms27177804","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T09:48:36Z","timestamp":1788256116000},"page":"7804","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Physiological and Transcriptomic Analysis of Shading Stress Responses in Calamus viminalis Seedlings"],"prefix":"10.3390","volume":"27","author":[{"given":"Benxue","family":"Chen","sequence":"first","affiliation":[{"name":"College of Design, Zhoukou Normal University, Zhoukou 466001, China"},{"name":"Henan Key Laboratory of Crop Molecular Breeding and Bioreactor, Zhoukou 466001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Wu","sequence":"additional","affiliation":[{"name":"Zhengzhou Academy of Agricultural Science and Technology, Zhengzhou 450007, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Du","sequence":"additional","affiliation":[{"name":"College of Design, Zhoukou Normal University, Zhoukou 466001, China"},{"name":"Henan Key Laboratory of Crop Molecular Breeding and Bioreactor, Zhoukou 466001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Wei","sequence":"additional","affiliation":[{"name":"College of Design, Zhoukou Normal University, Zhoukou 466001, China"},{"name":"Henan Key Laboratory of Crop Molecular Breeding and Bioreactor, Zhoukou 466001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanbing","family":"Li","sequence":"additional","affiliation":[{"name":"College of Design, Zhoukou Normal University, Zhoukou 466001, China"},{"name":"Henan Key Laboratory of Crop Molecular Breeding and Bioreactor, Zhoukou 466001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanglu","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory for Bamboo and Rattan, International Center for Bamboo and Rattan, Beijing 100102, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"key":"ref_1","first-page":"320","article-title":"Resources and Distribution of Rattan in China","volume":"33","author":"Lin","year":"2015","journal-title":"Plant Sci. 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