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J. Mol. Sci.","IJMS"],"abstract":"<jats:p>The RNA cargo of extracellular vesicles (EVs) has been investigated to search for circulating biomarkers possibly associated with a given disease. In this direction, vesicular small RNAs like microRNAs (miRNAs) showed great potential e.g., in monitoring neurodegenerative diseases and their clinical signs. In this exploratory cross-sectional study, we looked for miRNAs possibly associated with age-related cognitive impairment. Using a high processivity and sensitive OpenArray technology, we analyzed the expression of 223 miRNAs in plasmatic total EV-enriched fractions from 43 subjects older than 65 years who received neuropsychological examination. In a smaller cohort of 17 subjects, we verified any miRNA discrepancies between EV-associated and unfractionated plasma samples. A set of candidate miRNAs showed reduced expression in EV-associated fractions from cognitively impaired (n. 12) compared to cognitively preserved (n. 23) subjects. In particular, the expression of 15 of them (miR-106b-5p, miR-128-3p, miR-15a-5p, miR-16-5p, miR-17-5p, miR-191-5p, miR-192-5p, miR-199a-3p, miR-221-3p, miR-222-3p, miR-223-3p, miR-22-3p, miR-26a-5p, miR-335-5p, miR-505-3p) correlated with the MMSE score. Pathways of neurodegeneration (ND), such as PI3K-Akt, Ras\/MAPK, and Wnt signaling, resulted significantly involved. Furthermore, 18 miRNAs were implicated in metal ion binding processes, thus supporting the key role of this molecular mechanism in ND-associated cognitive dysfunction. The paired analysis of EV-plasma samples reported the overall upregulation of most of the 223 miRNAs in plasma samples, whereas miR-424-5p, miR-505-3p, miR-192-5p, miR-338-3p and miR-1271-5p were almost stable in the two fractions and miR-205-5p and miR-28-5p were significantly upregulated in EVs compared to plasma. In our view, these findings support the potential relevance of vesicular-associated miRNAs as candidates of age-related cognitive dysfunctions, although for clinical practice some methodological issues may arise, i.e., the feasibility of total EVs extraction. Further confirmations are needed, e.g., the complete characterization of EVs\u2019 cargo and origin, and validation in larger samples.<\/jats:p>","DOI":"10.3390\/ijms27177838","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T15:08:43Z","timestamp":1788275323000},"page":"7838","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Low-Sample Input miRNA Profiling in Extracellular Vesicles-Enriched Plasma Fractions from Older Adults With\/out Cognitive Impairment"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3768-6352","authenticated-orcid":false,"given":"Arianna","family":"Consiglio","sequence":"first","affiliation":[{"name":"Institute for Biomedical Technologies, National Research Council, Bari Unit, 70125 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9546-6231","authenticated-orcid":false,"given":"Ylenia","family":"Antonacci","sequence":"additional","affiliation":[{"name":"Institute for Biomedical Technologies, National Research Council, Bari Unit, 70125 Bari, Italy"},{"name":"Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies, National Research Council, 70125 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Claudia","family":"Lograno","sequence":"additional","affiliation":[{"name":"Alzheimer\u2019s Association Bari, Confederated to Alzheimer\u2019s Federation Italy, 70124 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Claudia","family":"Chiapparino","sequence":"additional","affiliation":[{"name":"Alzheimer\u2019s Association Bari, Confederated to Alzheimer\u2019s Federation Italy, 70124 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Katia","family":"Pinto","sequence":"additional","affiliation":[{"name":"Alzheimer\u2019s Association Bari, Confederated to Alzheimer\u2019s Federation Italy, 70124 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"supervision","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Apollonia","family":"Tullo","sequence":"additional","affiliation":[{"name":"Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies, National Research Council, 70125 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"supervision","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4338-0392","authenticated-orcid":false,"given":"Maria","family":"Liguori","sequence":"additional","affiliation":[{"name":"Institute for Biomedical Technologies, National Research Council, Bari Unit, 70125 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1864","DOI":"10.1038\/s41467-021-22126-y","article-title":"Quantitative characterization of extracellular vesicle uptake and content delivery within mammalian cells","volume":"12","author":"Bonsergent","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1038\/ncb1596","article-title":"Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells","volume":"9","author":"Valadi","year":"2007","journal-title":"Nat. Cell Biol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"13368","DOI":"10.1073\/pnas.0403453101","article-title":"Identification and proteomic profiling of exosomes in human urine","volume":"101","author":"Pisitkun","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1093\/intimm\/dxh267","article-title":"Exosomal-like vesicles are present in human blood plasma","volume":"17","author":"Caby","year":"2005","journal-title":"Int. Immunol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"578064","DOI":"10.1016\/j.jneuroim.2023.578064","article-title":"Emerging role of extracellular vesicles in multiple sclerosis: From cellular surrogates to pathogenic mediators and beyond","volume":"377","author":"Palacio","year":"2023","journal-title":"J. Neuroimmunol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.neuroscience.2018.04.003","article-title":"Cell-to-cell Communication by Extracellular Vesicles: Focus on Microglia","volume":"405","author":"Paolicelli","year":"2019","journal-title":"Neuroscience"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/1750-1326-7-42","article-title":"Exosomal cell-to-cell transmission of alpha synuclein oligomers","volume":"7","author":"Danzer","year":"2012","journal-title":"Mol. Neurodegener."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1038\/cdd.2013.180","article-title":"Microglia convert aggregated amyloid-\u03b2 into neurotoxic forms through the shedding of microvesicles","volume":"21","author":"Joshi","year":"2013","journal-title":"Cell Death Differ."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Guix, F.X., Corbett, G.T., Cha, D.J., Mustapic, M., Liu, W., Mengel, D., Chen, Z., Aikawa, E., Young-Pearse, T., and Kapogiannis, D. (2018). Detection of Aggregation-Competent Tau in Neuron-Derived Extracellular Vesicles. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19030663"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e2317402121","DOI":"10.1073\/pnas.2317402121","article-title":"Microglia-derived extracellular vesicles trigger age-related neurodegeneration upon DNA damage","volume":"121","author":"Arvanitaki","year":"2024","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.1016\/S1474-4422(12)70191-6","article-title":"Cognitive reserve in ageing and Alzheimer\u2019s disease","volume":"11","author":"Stern","year":"2012","journal-title":"Lancet Neurol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Liu, Y., Lu, G., Liu, L., He, Y., and Gong, W. (2024). Cognitive reserve over the life course and risk of dementia: A systematic review and meta-analysis. Front. Aging Neurosci., 16.","DOI":"10.3389\/fnagi.2024.1358992"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.colsurfb.2018.02.006","article-title":"Counting of peripheral extracellular vesicles in Multiple Sclerosis patients by an improved nanoplasmonic assay and dynamic light scattering","volume":"168","author":"Mallardi","year":"2018","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"12983","DOI":"10.1021\/acsomega.4c09363","article-title":"Hetero Sandwich Immunoassay as Tool to Probe the Composition of the Extracellular Vesicles Membranes: The Case Study of L1CAM Localization","volume":"10","author":"Mateos","year":"2025","journal-title":"ACS Omega"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Nuzziello, N., Blonda, M., Licciulli, F., Liuni, S., Amoruso, A., Valletti, A., Consiglio, A., Avolio, C., and Liguori, M. (2017). Molecular Characterization of Peripheral Extracellular Vesicles in Clinically Isolated Syndrome: Preliminary Suggestions from a Pilot Study. Med. Sci., 5.","DOI":"10.3390\/medsci5030019"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.1186\/s12967-024-05852-x","article-title":"MST1, a novel therapeutic target for Alzheimer\u2019s disease, regulates mitochondrial homeostasis by mediating mitochondrial DNA transcription and the PI3K-Akt-ROS pathway","volume":"22","author":"Cui","year":"2024","journal-title":"J. Transl. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1146\/annurev-genom-091416-035332","article-title":"Cognitive Dysfunctions in Intellectual Disabilities: The Contributions of the Ras-MAPK and PI3K-AKT-mTOR Pathways","volume":"18","author":"Borrie","year":"2017","journal-title":"Annu. Rev. Genom. Hum. Genet."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"107558","DOI":"10.1016\/j.nlm.2021.107558","article-title":"Role of Wnt signaling in synaptic plasticity and memory","volume":"187","author":"Narvaes","year":"2022","journal-title":"Neurobiol. Learn. Mem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.yhbeh.2015.06.021","article-title":"The endocrine dyscrasia that accompanies menopause and andropause induces aberrant cell cycle signaling that triggers re-entry of post-mitotic neurons into the cell cycle, neurodysfunction, neurodegeneration and cognitive disease","volume":"76","author":"Atwood","year":"2015","journal-title":"Horm. Behav."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Martinez, T.P., Larsen, M.E., Sullivan, E., Woolfrey, K.M., and Dell\u2019Acqua, M.L. (2024). Amyloid-\u03b2-Induced Dendritic Spine Elimination Requires Ca2+-Permeable AMPA Receptors, AKAP-Calcineurin-NFAT Signaling, and the NFAT Target Gene Mdm2. eNeuro, 11.","DOI":"10.1523\/ENEURO.0175-23.2024"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2097","DOI":"10.1002\/acn3.70143","article-title":"Cognitive Resilience in Apolipoprotein \u03b54 Carrier Women Predicted by Neuron-Derived Extracellular Vesicles","volume":"12","author":"Manolopoulos","year":"2025","journal-title":"Ann. Clin. Transl. Neurol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1038\/s41398-018-0268-6","article-title":"Schizophrenia-related cognitive dysfunction in the Cyclin-D2 knockout mouse model of ventral hippocampal hyperactivity","volume":"8","author":"Grimm","year":"2018","journal-title":"Transl. Psychiatry"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1042\/BST20200109","article-title":"Mutations in genes encoding regulators of mRNA decapping and translation initiation: Links to intellectual disability","volume":"48","author":"Weil","year":"2020","journal-title":"Biochem. Soc. Trans."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e13465","DOI":"10.1111\/acel.13465","article-title":"YAP prevents premature senescence of astrocytes and cognitive decline of Alzheimer\u2019s disease through regulating CDK6 signaling","volume":"20","author":"Xu","year":"2021","journal-title":"Aging Cell"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"23670","DOI":"10.1039\/D5RA02993G","article-title":"Profiling characteristics of plasma exosomal miRNAs across cognitive stages: From normalcy to mild cognitive impairment and Alzheimer\u2019s disease","volume":"15","author":"Chen","year":"2025","journal-title":"RSC Adv."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/S1474-4422(21)00361-6","article-title":"Blood-based biomarkers for Alzheimer\u2019s disease: Towards clinical implementation","volume":"21","author":"Teunissen","year":"2022","journal-title":"Lancet Neurol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Rybak-Wolf, A., and Plass, M. (2021). RNA Dynamics in Alzheimer\u2019s Disease. Molecules, 26.","DOI":"10.3390\/molecules26175113"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Sproviero, D., Gagliardi, S., Zucca, S., Arigoni, M., Giannini, M., Garofalo, M., Olivero, M., Dell\u2019orco, M., Pansarasa, O., and Bernuzzi, S. (2021). Different miRNA Profiles in Plasma Derived Small and Large Extracellular Vesicles from Patients with Neurodegenerative Diseases. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22052737"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"4952","DOI":"10.1002\/alz.13055","article-title":"MicroRNA expression in extracellular vesicles as a novel blood-based biomarker for Alzheimer\u2019s disease","volume":"19","author":"Kumar","year":"2023","journal-title":"Alzheimers Dement."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1177\/1533317517698793","article-title":"Supporting Simple Activity Engagement in Persons with Moderate to Severe Alzheimer\u2019s Disease Through a Technology-Aided Program","volume":"32","author":"Lancioni","year":"2017","journal-title":"Am. J. Alzheimer\u2019s Dis. Other Dementiasr"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1111\/joim.12190","article-title":"Mild cognitive impairment: A concept in evolution","volume":"275","author":"Petersen","year":"2014","journal-title":"J. Intern. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.1007\/s00213-025-06763-5","article-title":"MiR-223-3p inhibits hippocampal neurons injury and exerts anti- anxiety\/depression-like behaviors by directly targeting NLRP3","volume":"242","author":"Wang","year":"2025","journal-title":"Psychopharmacology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"9393","DOI":"10.1038\/s41598-019-45687-x","article-title":"Circulatory miR-223-3p Discriminates Between Parkinson\u2019s and Alzheimer\u2019s Patients","volume":"9","author":"Mancuso","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"851","DOI":"10.3233\/JAD-142214","article-title":"Linking Genetics of Brain Changes to Alzheimer\u2019s Disease: Sparse Whole Genome Association Scan of Regional MRI Volumes in the ADNI and AddNeuroMed Cohorts","volume":"45","author":"Khondoker","year":"2015","journal-title":"J. Alzheimer\u2019s Dis."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1843","DOI":"10.1016\/j.jmb.2019.01.018","article-title":"Metal Toxicity Links to Alzheimer\u2019s Disease and Neuroinflammation","volume":"431","author":"Huat","year":"2019","journal-title":"J. Mol. Biol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.1007\/s11011-021-00765-w","article-title":"Role of metals in Alzheimer\u2019s disease","volume":"36","author":"Das","year":"2021","journal-title":"Metab. Brain Dis."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3250","DOI":"10.1021\/acschemneuro.1c00444","article-title":"miR-29a\/b1 Regulates BACE1 in Aluminum-Induced A\u03b2 Deposition in Vitro","volume":"12","author":"Li","year":"2021","journal-title":"ACS Chem. Neurosci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"15966","DOI":"10.1038\/s41598-022-20305-5","article-title":"Lead-exposure associated miRNAs in humans and Alzheimer\u2019s disease as potential biomarkers of the disease and disease processes","volume":"12","author":"Wen","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.neuro.2017.04.007","article-title":"Environmental neurotoxicant manganese regulates exosome-mediated extracellular miRNAs in cell culture model of Parkinson\u2019s disease: Relevance to \u03b1-synuclein misfolding in metal neurotoxicity","volume":"64","author":"Harischandra","year":"2018","journal-title":"NeuroToxicology"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Visconte, C., Fenoglio, C., Serpente, M., Muti, P., Sacconi, A., Rigoni, M., Arighi, A., Borracci, V., Arcaro, M., and Arosio, B. (2023). Altered Extracellular Vesicle miRNA Profile in Prodromal Alzheimer\u2019s Disease. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241914749"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"107132","DOI":"10.1016\/j.nbd.2025.107132","article-title":"Comparison of neuron-derived extracellular vesicles miRNA profile between patients with behavioural variant frontotemporal dementia and primary psychiatric disorders","volume":"216","author":"Giudici","year":"2025","journal-title":"Neurobiol. Dis."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"106867","DOI":"10.1016\/j.nbd.2025.106867","article-title":"Differential miRNA expression in neural-enriched extracellular vesicles as potential biomarker for frontotemporal dementia and bipolar disorder","volume":"208","author":"Serpente","year":"2025","journal-title":"Neurobiol. Dis."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Sandau, U.S., McFarland, T.J., Smith, S.J., Galasko, D.R., Quinn, J.F., and Saugstad, J.A. (2022). Differential Effects of APOE Genotype on MicroRNA Cargo of Cerebrospinal Fluid Extracellular Vesicles in Females with Alzheimer\u2019s Disease Compared to Males. Front. Cell Dev. Biol., 10.","DOI":"10.3389\/fcell.2022.864022"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"12302","DOI":"10.1002\/jev2.12302","article-title":"Platelet removal from human blood plasma improves detection of extracellular vesicle-associated miRNA","volume":"12","author":"Bracht","year":"2023","journal-title":"J. Extracell. Vesicles"},{"key":"ref_45","first-page":"S235","article-title":"Small RNA Profiles of Brain Tissue-Derived Extracellular Vesicles in Alzheimer\u2019s Disease","volume":"99","author":"Huang","year":"2024","journal-title":"J. Alzheimer\u2019s Dis."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/0022-3956(75)90026-6","article-title":"\u201cMini-mental state\u201d. A practical method for grading the cognitive state of patients for the clinician","volume":"12","author":"Folstein","year":"1975","journal-title":"J. Psychiatr. Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1111\/j.1468-1331.1996.tb00423.x","article-title":"Mini-Mental State Examination: A normative study in Italian elderly population","volume":"3","author":"Magni","year":"1996","journal-title":"Eur. J. Neurol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1001\/jama.1963.03060120024016","article-title":"Studies of illness in the aged. the index of ADL: A standardized measure of biological and psychosocial function","volume":"185","author":"Katz","year":"1963","journal-title":"JAMA"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1093\/geront\/9.3_Part_1.179","article-title":"Assessment of older people: Self-maintaining and instrumental activities of daily living","volume":"9","author":"Lawton","year":"1969","journal-title":"Gerontologist"},{"key":"ref_50","unstructured":"American Psychiatric Association (2015). Diagnostic and Statistical Manual of Mental Disorders, Raffaello Cortina. [5th ed.]. DSM-5."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1016\/0895-4356(94)90122-8","article-title":"The mini-mental state examination score and the clinical diagnosis of dementia","volume":"47","author":"Kukull","year":"1994","journal-title":"J. Clin. Epidemiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"e69","DOI":"10.1002\/cphg.69","article-title":"MicroRNA Isolation from Plasma for Real-Time qPCR Array","volume":"99","author":"Witvrouwen","year":"2018","journal-title":"Curr. Protoc. Hum. Genet."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"e12404","DOI":"10.1002\/jev2.12404","article-title":"Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches","volume":"13","author":"Welsh","year":"2024","journal-title":"J. Extracell. Vesicles"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1080\/09537104.2022.2027903","article-title":"Does hsa-miR-223-3p from platelet-derived extracellular vesicles regulate tissue factor expression in monocytic cells?","volume":"33","author":"Collier","year":"2022","journal-title":"Platelets"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Gevaert, A.B., Witvrouwen, I., Vrints, C.J., Heidbuchel, H., Van Craenenbroeck, E.M., Van Laere, S.J., and Van Craenenbroeck, A.H. (2018). MicroRNA profiling in plasma samples using qPCR arrays: Recommendations for correct analysis and interpretation. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0193173"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/17\/7838\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T13:52:14Z","timestamp":1788529934000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/17\/7838"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,1]]},"references-count":55,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["ijms27177838"],"URL":"https:\/\/doi.org\/10.3390\/ijms27177838","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,1]]}}}