{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T11:22:33Z","timestamp":1788434553080,"version":"build-2803163510"},"reference-count":53,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Doxorubicin (DOX) is an antineoplastic agent commonly used in cancer therapy that is known for its testicular toxicity. DOX-induced testicular injury involves inflammation, reactive oxygen species, and oxidative stress. Centella asiatica exhibits anti-inflammatory and antioxidant properties. The present experimental study aimed to determine the potential protective efficacy of Centella asiatica in a rat model of doxorubicin-induced testicular toxicity. Rats were randomly assigned to four groups: healthy control (HC), CA, DOX, and CA + DOX (CADX). An oral dose of 200 mg\/kg Centella asiatica was administered to the CA and CADX groups. The HC and DOX groups received saline. One hour later, DOX (7.5 mg\/kg) was intraperitoneally injected into the DOX and CADX groups on days 1, 4, and 7. Centella asiatica markedly attenuated the DOX-induced increase in malondialdehyde, nitric oxide, and pro-inflammatory cytokine levels, while preventing the decrease in superoxide dismutase activity in testicular tissue (p &lt; 0.001). Centella asiatica reduced the severity of DOX-induced morphological damage in testicular tissue (p &lt; 0.05). Moreover, it partially restored reproductive hormone function. In conclusion, Centella asiatica demonstrates significant potential in mitigating DOX-induced oxidative damage, attenuating testicular injury and preserving the functional integrity of the male reproductive system.<\/jats:p>","DOI":"10.3390\/ijms27177883","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T11:08:53Z","timestamp":1788433733000},"page":"7883","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Investigation of the Protective Effect of Centella asiatica Against Doxorubicin-Induced Testicular Damage in Rats"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0506-0777","authenticated-orcid":false,"given":"Fevzi","family":"Bedir","sequence":"first","affiliation":[{"name":"Department of Urology, Health Sciences University, Erzurum Regional Training and Research Hospital, Erzurum 25240, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0128-7990","authenticated-orcid":false,"given":"Zeynep","family":"Suleyman","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Nursing, Faculty of Health Sciences, Erzincan Binali Yildirim University, Erzincan 24030, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Huseyin","family":"Kocaturk","sequence":"additional","affiliation":[{"name":"Department of Urology, Health Sciences University, Erzurum Regional Training and Research Hospital, Erzurum 25240, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"validation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5691-0666","authenticated-orcid":false,"given":"Mehmet Sefa","family":"Altay","sequence":"additional","affiliation":[{"name":"Department of Urology, Health Sciences University, Erzurum Regional Training and Research Hospital, Erzurum 25240, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"validation","vocabulary":"credit"}]},{"given":"Ferda","family":"Keskin Cimen","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan 24030, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5756-3459","authenticated-orcid":false,"given":"Durdu","family":"Altuner","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan 24030, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"data-curation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-5109-7052","authenticated-orcid":false,"given":"Mansura","family":"Babayeva","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Azerbaijan Medical University, Baku AZ1022, Azerbaijan"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9239-4099","authenticated-orcid":false,"given":"Halis","family":"Suleyman","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan 24030, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1023\/A:1007094214460","article-title":"Adriamycin-induced heart failure: Mechanism; modulation","volume":"207","author":"Singal","year":"2000","journal-title":"Mol. 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