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J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Malformations of cortical development frequently underlie drug-resistant epilepsy, yet little is known about how malformed cortical networks terminate epileptiform activity. In acute cortical slices from juvenile male Wistar rats with a focal freeze lesion, we compared the microgyrus and paramicrogyral zone during low-Mg2+\/gabazine-induced epileptiform activity. Discharges terminated earlier in the microgyrus than in the paramicrogyral zone (median, 268 vs. 496 ms), without detectable regional differences in peak discharge-associated inward current or the weighted decay time constant of extracellular K+ transients. In separate voltage-clamp recordings, the transition to a slow post-discharge outward current occurred earlier in microgyral neurons, and the current peaked sooner and showed a smaller normalized late component. GABAB receptor blockade with CGP-55845 prolonged discharges and preferentially disrupted the faster post-peak current decay in the microgyrus. Intracellular QX-314, used to probe a postsynaptic component, eliminated detectable regional differences in outward-current kinetics. Gabbr1 and Gabbr2 mRNA abundance did not differ detectably between the microgyrus and contralateral cortex. Overall, the findings support a postsynaptic GABAB-dependent contribution to earlier epileptiform discharge termination within the microgyrus. More broadly, malformation-associated reorganization includes local negative-feedback processes that constrain pathological network persistence alongside mechanisms that promote hyperexcitability.<\/jats:p>","DOI":"10.3390\/ijms27177896","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T13:50:44Z","timestamp":1788529844000},"page":"7896","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["GABAB-Dependent Negative Feedback Contributes to Earlier Epileptiform Discharge Termination Within Malformed Cortex"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7098-4768","authenticated-orcid":false,"given":"Dmitry V.","family":"Amakhin","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Mechanisms of Neural Interactions, Sechenov Institute of Evolutionary Physiology and Biochemistry of RAS, 194223 Saint Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7690-583X","authenticated-orcid":false,"given":"Elena B.","family":"Soboleva","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Mechanisms of Neural Interactions, Sechenov Institute of Evolutionary Physiology and Biochemistry of RAS, 194223 Saint Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3790-2396","authenticated-orcid":false,"given":"Anna A.","family":"Kovalenko","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Mechanisms of Neural Interactions, Sechenov Institute of Evolutionary Physiology and Biochemistry of RAS, 194223 Saint Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7049-4478","authenticated-orcid":false,"given":"Tatiana Yu.","family":"Postnikova","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Mechanisms of Neural Interactions, Sechenov Institute of Evolutionary Physiology and Biochemistry of RAS, 194223 Saint Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4503-0647","authenticated-orcid":false,"given":"Aleksey V.","family":"Zaitsev","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Mechanisms of Neural Interactions, Sechenov Institute of Evolutionary Physiology and Biochemistry of RAS, 194223 Saint Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"47","DOI":"10.31887\/DCNS.2008.10.1\/rjleventer","article-title":"Malformations of Cortical Development and Epilepsy","volume":"10","author":"Leventer","year":"2008","journal-title":"Dialogues Clin. 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