{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T05:14:36Z","timestamp":1788671676151,"version":"build-2803163510"},"reference-count":31,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Rugulopteryx okamurae is an aggressively invasive seaweed whose proliferation along the Moroccan coastline represents both an ecological problem and an underexploited source of biomass. This work highlights the isolation of sodium alginate from R. okamurae under four combinations of acid pretreatment and alkaline (carbonate) extraction conditions. The extracted alginates were characterized by determining the extraction yield and analyzing their structural features using Fourier-transform infrared spectroscopy (FTIR) and proton nuclear magnetic resonance (1H NMR). Alginate yields ranged from 11 to ~16% (dry weight), and the highest yield 15.85 \u00b1 0.66% dw was obtained with acidification treatments of 2 h at 40 \u00b0C and alkaline extraction at 60 \u00b0C over 5 h. FTIR analysis confirmed the sodium alginate structure and suggested the absence of detectable sulfate polysaccharide (fucoidan) contamination, as evidenced by the absence of a S=O band. 1H NMR analysis revealed a guluronate rich polymer within the four used protocols, highlighting its potential to form a strong and rigid gel. The present study highlights the alien R. okamurae as a viable source of high guluronate sodium alginate and clarifies how extraction conditions might shape its yields.<\/jats:p>","DOI":"10.3390\/ijms27177898","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T13:50:44Z","timestamp":1788529844000},"page":"7898","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Valorization of Beach-Cast Biomass from the Invasive Seaweed Rugulopteryx okamurae on the Moroccan Coast: A Comparative Assessment of Extraction Conditions for Sodium Alginate Recovery"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-2587-1274","authenticated-orcid":false,"given":"Khansae","family":"Kamal","sequence":"first","affiliation":[{"name":"Phycology, Blue Biodiversity and Biotechnology RU, Laboratory of Plant Biotechnology, Ecology and Ecosystem Valorization\u2014CNRST Labeled Research Unit N\u00b010, Faculty of Sciences, University Chouaib Doukkali, P.O. 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Box 20, El Jadida 24000, Morocco"},{"name":"Materials and Transformations Unit, University of Lille, CNRS, INRAE, Centrale Lille, UMR 8207-UMET, F-59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5240-7804","authenticated-orcid":false,"given":"Charafeddine","family":"Jama","sequence":"additional","affiliation":[{"name":"Materials and Transformations Unit, University of Lille, CNRS, INRAE, Centrale Lille, UMR 8207-UMET, F-59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"software","vocabulary":"credit"}]},{"given":"Abdellatif","family":"Chaouti","sequence":"additional","affiliation":[{"name":"Phycology, Blue Biodiversity and Biotechnology RU, Laboratory of Plant Biotechnology, Ecology and Ecosystem Valorization\u2014CNRST Labeled Research Unit N\u00b010, Faculty of Sciences, University Chouaib Doukkali, P.O. 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Appl. Phycol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1111\/jpy.70084","article-title":"A taxonomic sudoku: Resolving the long-standing puzzle of Japanese Dictyoteae (Dictyotales, Phaeophyceae) with new records and biogeographic insights","volume":"61","author":"Vieira","year":"2025","journal-title":"J. Phycol."},{"key":"ref_3","first-page":"92","article-title":"Occurrence of the invasive brown seaweed Rugulopteryx okamurae (E.Y. Dawson)","volume":"1","author":"Idhalla","year":"2018","journal-title":"Mediterr. Fish. Aquac. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"103722","DOI":"10.1016\/j.aquabot.2023.103722","article-title":"Recent occurrence and expansion of the non-indigenous alga Rugulopteryx okamurae in Morocco (Mediterranean and Atlantic shores)","volume":"190","author":"Hassoun","year":"2024","journal-title":"Aquat. Bot."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"385","DOI":"10.3391\/bir.2024.13.2.08","article-title":"Dictyota rugulopteryx (Dictyotales, Ochrophyta) continues to expand: First record in Italy","volume":"13","author":"Bellissimo","year":"2024","journal-title":"BioInvasions Rec."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"107135","DOI":"10.1016\/j.ecolind.2020.107135","article-title":"Impacts of the non-indigenous seaweed Rugulopteryx okamurae on a Mediterranean coralligenous community (Strait of Gibraltar): The role of long-term monitoring","volume":"121","author":"Maestre","year":"2021","journal-title":"Ecol. Indic."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"113358","DOI":"10.1016\/j.marpolbul.2022.113358","article-title":"Dramatic changes in the structure of shallow-water marine benthic communities following the invasion by Rugulopteryx okamurae (Dictyotales, Ochrophyta) in Azores (NE Atlantic)","volume":"175","author":"Faria","year":"2022","journal-title":"Mar. Pollut. Bull."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Santana, I., F\u00e9lix, M., Guerrero, A., and Bengoechea, C. (2022). Processing and characterization of bioplastics from the invasive seaweed Rugulopteryx okamurae. Polymers, 14.","DOI":"10.3390\/polym14020355"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Barcellos, L., Pham, C.K., Menezes, G., Bettencourt, R., Rocha, N., Carvalho, M., and Felgueiras, H.P. (2023). A concise review on the potential applications of Rugulopteryx okamurae macroalgae. Mar. Drugs, 21.","DOI":"10.3390\/md21010040"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1007\/s12649-022-01849-z","article-title":"Composting the invasive toxic seaweed Rugulopteryx okamurae using five invertebrate species, and a mini-review on composting macroalgae","volume":"14","author":"Loring","year":"2023","journal-title":"Waste Biomass Valorization"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Santana, I., F\u00e9lix, M., and Bengoechea, C. (2024). Feasibility of invasive brown seaweed Rugulopteryx okamurae as source of alginate: Characterization of products and evaluation of derived gels. Polymers, 16.","DOI":"10.3390\/polym16050702"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Rinc\u00f3n-Cervera, M.A., de Burgos-Navarro, I., Chileh-Chelh, T., Belarbi, E.H., \u00c1lvarez-Corral, M., Carmona-Fern\u00e1ndez, M., Ezzaitouni, M., and Guil-Guerrero, J.L. (2024). The agronomic potential of the invasive brown seaweed Rugulopteryx okamurae: Optimisation of alginate, mannitol, and phlorotannin extraction. Plants, 13.","DOI":"10.3390\/plants13243539"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1021\/bm2013026","article-title":"Alginate sequencing: An analysis of block distribution in alginates using specific alginate-degrading enzymes","volume":"13","author":"Aarstad","year":"2012","journal-title":"Biomacromolecules"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/s11947-025-04096-x","article-title":"Sustainable valorization of alginate: A review of green extraction, structure\u2013function relationships, and next-generation food applications","volume":"19","author":"Felicia","year":"2026","journal-title":"Food Bioprocess Technol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1081\/DDC-120003853","article-title":"Alginate in drug delivery systems","volume":"28","author":"Karlsen","year":"2002","journal-title":"Drug Dev. Ind. Pharm."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Belattmania, Z., Kaidi, S., Atouani, S.E., Katif, C., Bentiss, F., Jama, C., Reani, A., Sabour, B., and Vasconcelos, V. (2020). Isolation and FTIR-ATR and 1H NMR characterization of alginates from the main alginophyte species of the Atlantic coast of Morocco. Molecules, 25.","DOI":"10.3390\/molecules25184335"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.progpolymsci.2011.06.003","article-title":"Alginate: Properties and biomedical applications","volume":"37","author":"Lee","year":"2012","journal-title":"Prog. Polym. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2067","DOI":"10.1016\/j.carres.2007.05.022","article-title":"Extraction and physicochemical characterization of Sargassum vulgare alginate from Brazil","volume":"342","author":"Torres","year":"2007","journal-title":"Carbohydr. Res."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Ferreira-Anta, T., Fl\u00f3rez-Fern\u00e1ndez, N., Torres, M.D., Maz\u00f3n, J., and Dom\u00ednguez, H. (2023). Microwave-assisted hydrothermal processing of Rugulopteryx okamurae. Mar. Drugs, 21.","DOI":"10.3390\/md21060319"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"138241","DOI":"10.1016\/j.foodchem.2023.138241","article-title":"Characterization of the invasive macroalgae Rugulopteryx okamurae for potential biomass valorisation","volume":"440","author":"Calvo","year":"2024","journal-title":"Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/S0065-2318(08)60077-3","article-title":"Vibrational spectra of carbohydrates","volume":"44","author":"Mathlouthi","year":"1987","journal-title":"Adv. Carbohydr. Chem. Biochem."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Rashedy, S.H., Abd El Hafez, M.S.M., Dar, M.A., Cotas, J., and Pereira, L. (2021). Evaluation and characterization of alginate extracted from brown seaweed collected in the Red Sea. Appl. Sci., 11.","DOI":"10.3390\/app11146290"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1111\/pre.12135","article-title":"The invasive brown seaweed Sargassum muticum as new resource for alginate in Morocco: Spectroscopic and rheological characterization","volume":"64","author":"Bentiss","year":"2016","journal-title":"Phycol. Res."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Kamal, K., Belattmania, Z., Khaya, K., Chaouti, A., Bentiss, F., Jama, C., Stiger-Pouvreau, V., and Sabour, B. (2025). Characterization of alginate from drifted pelagic Sargassum natans and Sargassum fluitans along the Moroccan Atlantic coast. Phys. Chem., 5.","DOI":"10.3390\/physchem5040051"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Kaidi, S., Bentiss, F., Jama, C., Khaya, K., Belattmania, Z., Reani, A., and Sabour, B. (2022). Isolation and structural characterization of alginates from the kelp species Laminaria ochroleuca and Saccorhiza polyschides from the Atlantic coast of Morocco. Colloids Interfaces, 6.","DOI":"10.3390\/colloids6040051"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/S0008-6215(00)84051-3","article-title":"Study of the composition and sequence of uronate residues in alginates","volume":"68","author":"Grasdalen","year":"1979","journal-title":"Carbohydr. Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1007\/978-981-10-6910-9_2","article-title":"Alginate Production from Marine Macroalgae, with Emphasis on Kelp Farming","volume":"Volume 11","author":"Rehm","year":"2018","journal-title":"Alginates and Their Biomedical Applications"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"121557","DOI":"10.1016\/j.carbpol.2023.121557","article-title":"In-process epimerisation of alginates from Saccharina latissima, Alaria esculenta and Laminaria hyperborea","volume":"325","author":"Aachmann","year":"2024","journal-title":"Carbohydr. Polym."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"54","DOI":"10.6000\/1929-5995.2025.14.06","article-title":"Multifunctional characterization of fucoidan: Structural insights and efficient removal of toxic metal ions","volume":"14","author":"Alberto","year":"2025","journal-title":"Res. Updat. Polym. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Roca, M., Dunbar, M.B., Rom\u00e1n, A., Caballero, I., Zoffoli, M.L., Gernez, P., and Navarro, G. (2022). Monitoring the marine invasive alien species Rugulopteryx okamurae using unmanned aerial vehicles and satellites. Front. Mar. Sci., 9.","DOI":"10.3389\/fmars.2022.1004012"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1023\/A:1017015824822","article-title":"Population and alginate yield and quality assessment of four Sargassum species in Negros Island, central Philippines","volume":"398","author":"Calumpong","year":"1999","journal-title":"Hydrobiologia"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/17\/7898\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T04:30:39Z","timestamp":1788669039000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/17\/7898"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,4]]},"references-count":31,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["ijms27177898"],"URL":"https:\/\/doi.org\/10.3390\/ijms27177898","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,4]]}}}