{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T18:12:39Z","timestamp":1788545559978,"version":"build-2803163510"},"reference-count":29,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fund of scientific research innovation of The first affiliated hospital of Harbin medical university","award":["2025B05"],"award-info":[{"award-number":["2025B05"]}]},{"award":["2025B05"],"award-info":[{"award-number":["2025B05"]}],"id":[{"id":"https:\/\/ror.org\/05vy2sc54","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"Young Scientists Fund of the National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82400523"],"award-info":[{"award-number":["82400523"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["82400523"],"award-info":[{"award-number":["82400523"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Coronary artery disease (CAD) and non-obstructive azoospermia (NOA) have distinct aetiologies. We examined whether separately analysed public transcriptomic cohorts contained overlapping exploratory candidate signals without assuming a shared causal mechanism. We re-analysed five Gene Expression Omnibus datasets using differential-expression screening, weighted gene co-expression network analysis (WGCNA), an archived neural-network candidate ranking, xCell enrichment scoring, and single-cell transcriptomic mapping. Nominal differential-expression screening identified 978 CAD-associated and 2562 NOA-associated candidate transcripts. WGCNA showed a moderate correlation between the MElightyellow module and NOA status (r = 0.58, p = 0.007) in GSE45887, a small, imbalanced, non-independent subset of GSE45885. An archived neural-network (NNET) ranking prioritised HSPA1B, PLCL2, ISLR2, STRN, and AQP7 for descriptive analyses; the ranking was generated from the same 20 specimens and is treated as heuristic. xCell produced marker-gene enrichment scores rather than direct measurements of cell abundance or function. Single-cell mapping was descriptive because GSE149512 combined heterogeneous NOA aetiologies with paediatric and adult comparator tissues. The analyses generate hypotheses from separate CAD and NOA cohorts. They do not establish a shared causal pathway, a sex- or age-independent association, temporal sequence, clinical diagnostic utility, or direct correspondence between testicular and coronary cell states.<\/jats:p>","DOI":"10.3390\/ijms27177909","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T17:25:56Z","timestamp":1788542756000},"page":"7909","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploratory Cross-Cohort Transcriptomic Comparison of Coronary Artery Disease and Non-Obstructive Azoospermia"],"prefix":"10.3390","volume":"27","author":[{"given":"Tengyu","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Cardiology, Cardiovascular Institute, The First Affiliated Hospital, Harbin Medical University, 23 Youzheng Street, Harbin 150001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengwei","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Cardiovascular Institute, The First Affiliated Hospital, Harbin Medical University, 23 Youzheng Street, Harbin 150001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Cardiovascular Institute, The First Affiliated Hospital, Harbin Medical University, 23 Youzheng Street, Harbin 150001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Cardiovascular Institute, The First Affiliated Hospital, Harbin Medical University, 23 Youzheng Street, Harbin 150001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruxin","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Radiology, The First Affiliated Hospital, Harbin Medical University, 23 Youzheng Street, Harbin 150001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyuan","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Cardiovascular Institute, The First Affiliated Hospital, Harbin Medical University, 23 Youzheng Street, Harbin 150001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1152\/physrev.00017.2015","article-title":"Male Reproductive Disorders and Fertility Trends: Influences of Environment and Genetic Susceptibility","volume":"96","author":"Skakkebaek","year":"2016","journal-title":"Physiol. 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