{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T18:12:41Z","timestamp":1788545561045,"version":"build-2803163510"},"reference-count":60,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"General Directorate of Research, Postgraduate Studies, and Service","award":["24036-EI"],"award-info":[{"award-number":["24036-EI"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>The excessive use of fungicides in agriculture has generated environmental problems, increased resistance in plant pathogenic fungi, and raised concerns for food safety and human health. This study evaluates silver-based nanoparticles (NPs) as a potential alternative for the control of plant pathogens. Ag2S-S Janus and AgNPs were synthesized through chemical reduction using non-toxic compounds, employing glucose as a reducing agent (0.30 and 0.90%) and gelatin as a passivating agent (0.50 and 1.00%), generating four treatments. The synthesized NPs showed average sizes ranging from 5 to 11 nm, with the smallest particles obtained in treatment T3 (5.90 \u00b1 0.30 nm) and the largest in T4 (10.34 \u00b1 0.46 nm). The antifungal and anti-oomycete activity of the NPs was evaluated in vitro against four microorganisms: Alternaria alternata, Colletotrichum boninense, Fusarium oxysporum, and Phytophthora capsici. Results showed complete inhibition (100%) of A. alternata and 60\u201370% inhibition of P. capsici in all treatments, demonstrating strong antimicrobial potential. Additionally, NPs were applied to tomato seedlings (Solanum lycopersicum) inoculated with Fusarium oxysporum f. sp. lycopersici. NPs were not phytotoxic and reduced disease severity (on a 1\u20132 scale) while promoting lesion healing.<\/jats:p>","DOI":"10.3390\/ijms27177916","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T17:25:56Z","timestamp":1788542756000},"page":"7916","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antifungal and Anti-Oomycete Potential of Ag2S-S Janus and Ag Nanoparticles Synthesized with Non-Toxic Agents and Their Application to Control Fusarium Wilt of Tomato"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4947-8262","authenticated-orcid":false,"given":"Rosa Elvira","family":"S\u00e1nchez-Fern\u00e1ndez","sequence":"first","affiliation":[{"name":"Laboratorio Nacional de Investigaci\u00f3n y Servicio Agroalimentario y Forestal (LANISAF), Universidad Aut\u00f3noma Chapingo, Carretera M\u00e9xico-Texcoco km 38.5, Chapingo, Texcoco 56230, Estado de M\u00e9xico, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7812-0948","authenticated-orcid":false,"given":"Mois\u00e9s Camacho","family":"Tapia","sequence":"additional","affiliation":[{"name":"Departamento de Parasitolog\u00eda, Universidad Aut\u00f3noma Chapingo, Carretera M\u00e9xico-Texcoco km 38.5, Chapingo, Texcoco 56230, Estado de M\u00e9xico, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8285-252X","authenticated-orcid":false,"given":"Daniel","family":"Canseco-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"SECIHTI-Laboratorio Nacional de Investigaci\u00f3n y Servicio Agroalimentario y Forestal, Universidad Aut\u00f3noma Chapingo, Texcoco 56230, Estado de M\u00e9xico, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guadalupe Stefanny","family":"Aguilar-Moreno","sequence":"additional","affiliation":[{"name":"Secretar\u00eda de Ciencia, Humanidades, Tecnolog\u00eda e Innovaci\u00f3n (SECIHTI)\u2014Universidad Aut\u00f3noma Chapingo, Carretera M\u00e9xico-Texcoco km 38.5, Chapingo, Texcoco 56230, Estado de M\u00e9xico, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3525-6519","authenticated-orcid":false,"given":"Miguel Angel","family":"Aguilar-M\u00e9ndez","sequence":"additional","affiliation":[{"name":"Instituto Polit\u00e9cnico Nacional, Centro de Investigaci\u00f3n en Ciencia Aplicada y Tecnolog\u0131\u00eda Avanzada, Legaria 694, Colonia Irrigaci\u00f3n, Mexico City 11500, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5697-4697","authenticated-orcid":false,"given":"Francisco","family":"Ascencio","sequence":"additional","affiliation":[{"name":"Laboratorio Nacional de Investigaci\u00f3n y Servicio Agroalimentario y Forestal (LANISAF), Universidad Aut\u00f3noma Chapingo, Carretera M\u00e9xico-Texcoco km 38.5, Chapingo, Texcoco 56230, Estado de M\u00e9xico, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leticia","family":"Rojas-Sandoval","sequence":"additional","affiliation":[{"name":"Departamento de Fitotecnia, Universidad Aut\u00f3noma Chapingo, Carretera M\u00e9xico-Texcoco km 38.5, Chapingo, Texcoco 56230, Estado de M\u00e9xico, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Navarro Cer\u00f3n","sequence":"additional","affiliation":[{"name":"Laboratorio Nacional de Investigaci\u00f3n y Servicio Agroalimentario y Forestal (LANISAF), Universidad Aut\u00f3noma Chapingo, Carretera M\u00e9xico-Texcoco km 38.5, Chapingo, Texcoco 56230, Estado de M\u00e9xico, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s10658-012-0144-9","article-title":"Climate change and potential future risks through wheat diseases: A review","volume":"136","author":"Juroszek","year":"2013","journal-title":"Eur. J. Plant Pathol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3347","DOI":"10.1021\/acs.est.8b04392","article-title":"Fungicides: An overlooked pesticide class?","volume":"53","author":"Zubrod","year":"2019","journal-title":"Environ. Sci. Technol."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"S\u00e1nchez-Torres, P. (2021). Molecular mechanisms underlying fungicide resistance in citrus postharvest green mold. J. Fungi, 7.","DOI":"10.3390\/jof7090783"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1049\/iet-nbt.2016.0213","article-title":"Biosynthesis of nanosilver using Chaetomium globosum and its application to control Fusarium wilt of tomato in the greenhouse","volume":"11","author":"Madbouly","year":"2017","journal-title":"IET Nanobiotechnol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1080\/15226514.2019.1634000","article-title":"Exposure of tomato (Lycopersicon esculentum) to silver nanoparticles and silver nitrate: Physiological and molecular response","volume":"22","author":"Noori","year":"2020","journal-title":"Int. J. Phytoremed."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1007\/s11706-020-0496-6","article-title":"Facile synthesis of asymmetric patchy Janus Ag\/Cu particles and study of their antifungal activity","volume":"14","author":"Biswas","year":"2020","journal-title":"Front. Mater. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1007\/s42452-021-04524-5","article-title":"Potential antifungal effects of silver nanoparticles (AgNPs) of different sizes against phytopathogenic Fusarium oxysporum f. sp. radicis-lycopersici (FORL) strains","volume":"3","author":"Akpinar","year":"2021","journal-title":"SN Appl. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1007\/s00203-023-03431-0","article-title":"Green synthesis of nanoparticles using botanicals and their application in management of fungal phytopathogens: A review","volume":"205","author":"Chauhan","year":"2023","journal-title":"Arch. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"105171","DOI":"10.1016\/j.fbio.2024.105171","article-title":"Janus nanoparticles synthesized from hydrophobic carbon dots and carboxymethyl cellulose: Novel antimicrobial additives for fresh food applications","volume":"62","author":"Razavi","year":"2024","journal-title":"Food Biosci."},{"key":"ref_10","first-page":"821","article-title":"Evaluation of the myco-toxicities of silver sulfide nanoparticles against phytopathogenic fungi","volume":"58","author":"Sidhu","year":"2021","journal-title":"Agric. Res. J."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Shoala, T. (2018). Positive impacts of nanoparticles in plant resistance against different stimuli. Nanobiotechnology Applications in Plant Protection, Springer International Publishing.","DOI":"10.1007\/978-3-319-91161-8_10"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Islam, A.S., Bhuiyan, R., Nihad, S.A.I., Akter, R., Khan, M.A.I., Akter, S., Islam, M.R., Khokon, M.A., and Latif, M.A. (2024). Green synthesis and characterization of silver nanoparticles and its efficacy against Rhizoctonia solani, a fungus causing sheath blight disease in rice. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0304817"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Slavin, Y.N., and Bach, H. (2022). Mechanisms of antifungal properties of metal nanoparticles. Nanomaterials, 12.","DOI":"10.3390\/nano12244470"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.fcr.2014.05.007","article-title":"Optimizing nutrient management strategies for rice\u2013wheat system in the Indo-Gangetic Plains of India and adjacent region for higher productivity, nutrient use efficiency and profits","volume":"164","author":"Singh","year":"2014","journal-title":"Field Crops Res."},{"key":"ref_15","first-page":"50","article-title":"Induction of chitinase and \u03b2-1, 3-glucanase PR proteins in tomato through liquid formulated Bacillus subtilis EPCO 16 against Fusarium wilt","volume":"1","author":"Ramyabharathi","year":"2012","journal-title":"J. Today\u2019s Biol. Sci. Res. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"73","DOI":"10.18633\/biotecnia.v22i3.952","article-title":"Silver nanoparticles coated with chitosan against Fusarium oxysporum causing the tomato wilt","volume":"22","author":"Juarez","year":"2020","journal-title":"Biotecnia"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1947","DOI":"10.1016\/S1001-0742(12)60301-5","article-title":"Comparison of the toxicity of silver nanoparticles and silver ions on the growth of terrestrial plant model Arabidopsis thaliana","volume":"25","author":"Qian","year":"2013","journal-title":"J. Environ. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.plgene.2017.07.005","article-title":"Differential phytotoxic responses of silver nitrate (AgNO3) and silver nanoparticle (AgNps) in Cucumis sativus L.","volume":"11","author":"Tripathi","year":"2017","journal-title":"Plant Gene"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.chemosphere.2014.03.056","article-title":"Physiological and molecular level effects of silver nanoparticles exposure in rice (Oryza sativa L.) seedlings","volume":"112","author":"Nair","year":"2014","journal-title":"Chemosphere"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"26644","DOI":"10.3390\/ijms161125980","article-title":"Plant responses to nanoparticle stress","volume":"16","author":"Hossain","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.3109\/17435390.2014.999139","article-title":"Silver sulfide nanoparticles (Ag2S-NPs) are taken up by plants and are phytotoxic","volume":"9","author":"Wang","year":"2015","journal-title":"Nanotoxicology"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1016\/j.scitotenv.2018.02.307","article-title":"Quantitative study of the capture of silver nanoparticles by several kinds of soils","volume":"630","year":"2018","journal-title":"Sci. Total Environ."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1835","DOI":"10.2134\/jeq2019.03.0098","article-title":"The Mobility of Silver Nanoparticles and Silver Ions in the Soil-Plant System","volume":"48","author":"Saleeb","year":"2019","journal-title":"J. Environ. Qual."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Yan, A., and Chen, Z. (2019). Impacts of silver nanoparticles on plants: A focus on the phytotoxicity and underlying mechanism. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20051003"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2525","DOI":"10.1007\/s11051-010-0145-6","article-title":"Synthesis and characterization of silver nanoparticles: Effect on phytopathogen Colletotrichum gloesporioides","volume":"13","year":"2011","journal-title":"J. Nanoparticle Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5942","DOI":"10.3390\/ma6125942","article-title":"Biosynthesis of silver nanoparticles using brown marine macroalga, Sargassum muticum aqueous extract","volume":"6","author":"Azizi","year":"2013","journal-title":"Materials"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"569","DOI":"10.2147\/IJN.S16867","article-title":"Green synthesis and characterization of gelatin-based and sugar-reduced silver nanoparticles","volume":"6","author":"Darroudi","year":"2011","journal-title":"Int. J. Nanomed."},{"key":"ref_28","first-page":"411","article-title":"Green synthesis of Ag2S nanoparticles: Effect of pH and capping agent on size and shape of Nps and their antibacterial activity","volume":"13","author":"Sibiya","year":"2018","journal-title":"Dig. J. Nanomater. Biostruct. (DJNB)"},{"key":"ref_29","unstructured":"Klug, H.P., and Alexander, L.E. (1974). X-Ray Diffraction Procedures: For Polycrystalline and Amorphous Materials, Wiley-VCH."},{"key":"ref_30","first-page":"42","article-title":"Green synthesis of gold nanoparticles, silver nanoparticles and gold-silver alloy nanoparticles using Ziziphus spina-christi leaf extracts and antibacterial activity against multidrug-resistant bacteria","volume":"84","author":"Alzahrani","year":"2022","journal-title":"Indian J. Pharm. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Sivera, M., Kvitek, L., Soukupova, J., Panacek, A., Prucek, R., Vecerova, R., and Zboril, R. (2014). Silver nanoparticles modified by gelatin with extraordinary pH stability and long-term antibacterial activity. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0103675"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"6346","DOI":"10.3390\/ijms12096346","article-title":"Fabrication and characterization of gelatin stabilized silver nanoparticles under UV-light","volume":"12","author":"Darroudi","year":"2011","journal-title":"Int. J. Mol. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"6857","DOI":"10.3390\/ijms15046857","article-title":"Preparation and characterization of gelatin nanofibers containing silver nanoparticles","volume":"15","author":"Jeong","year":"2014","journal-title":"Int. J. Mol. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"8281","DOI":"10.1038\/s41598-026-37330-3","article-title":"Ecofriendly synthesis of silver nanoparticles using Barleria gibsonii and evaluation of antibacterial antioxidant cytotoxic and catalytic activities","volume":"16","author":"Ali","year":"2026","journal-title":"Sci. Rep."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"103131","DOI":"10.1016\/j.nxmate.2026.103131","article-title":"Phyto-mediated green synthesis of silver nanoparticles for structural characterization, cytocompatibility, antioxidant and anti-inflammatory evaluation","volume":"13","author":"Mwamatope","year":"2026","journal-title":"Next Mater."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"100639","DOI":"10.1016\/j.nxnano.2026.100639","article-title":"Harnessing phytochemicals for nanotechnology: Antimicrobial silver nanoparticles from extracts of Curcuma caesia and Capparis multiflora","volume":"10","author":"Seema","year":"2026","journal-title":"Next Nanotechnol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Abbas, R., Luo, J., Qi, X., Naz, A., Khan, I.A., Liu, H., Yu, S., and Wei, J. (2024). Silver nanoparticles: Synthesis, structure, properties and applications. Nanomaterials, 14.","DOI":"10.3390\/nano14171425"},{"key":"ref_38","first-page":"65","article-title":"Silver and gold nanostructures: Antifungal property of different shapes of these nanostructures on Candida species","volume":"52","author":"Jebali","year":"2014","journal-title":"Med. Mycol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"100513","DOI":"10.1016\/j.apmt.2019.100513","article-title":"Janus nanoparticles: New generation of multifunctional nanocarriers in drug delivery, bioimaging and theranostics","volume":"18","author":"Rahiminezhad","year":"2020","journal-title":"Appl. Mater. Today"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Leslie, J.F., and Summerell, B.A. (2006). The Fusarium Laboratory Manual, Blackwell Publishing.","DOI":"10.1002\/9780470278376"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"15593258211044576","DOI":"10.1177\/15593258211044576","article-title":"Silver nanoparticles increase nitrogen, phosphorus, and potassium concentrations in leaves and stimulate root length and number of roots in tomato seedlings in a hormetic manner","volume":"19","year":"2021","journal-title":"Dose-Response"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1016\/j.sjbs.2019.06.002","article-title":"Fusarium oxysporum f. sp. lycopersici causal agent of vascular wilt disease of tomato: Biology to diversity\u2013A review","volume":"26","author":"Srinivas","year":"2019","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"103376","DOI":"10.1016\/j.bcab.2024.103376","article-title":"Green synthesis of silver nanoparticles for antifungal activity against tomato fusarium wilt caused by Fusarium oxysporum","volume":"61","author":"Tabassum","year":"2024","journal-title":"Biocatal. Agric. Biotechnol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"37","DOI":"10.21608\/bjas.2022.244584","article-title":"Control of tomato fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici by grafting and silver nanoparticles under greenhouse conditions","volume":"7","author":"Gomaa","year":"2022","journal-title":"Benha J. Appl. Sci."},{"key":"ref_45","first-page":"29","article-title":"Evaluaci\u00f3n de la fitotoxicidad del herbicida metribuzin en el cultivo de jitomate (Lycopersicum esculentum Mill.) y su eficiencia en el control de malezas","volume":"8","author":"Salas","year":"2013","journal-title":"Investig. Agrar."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1186\/s12951-014-0050-8","article-title":"Uptake, transport, distribution and bio-effects of SiO2 nanoparticles in Bt-transgenic cotton","volume":"12","author":"Le","year":"2014","journal-title":"J. Nanobiotechnol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1039\/D1EN00870F","article-title":"Uptake, translocation, and transformation of silver nanoparticles in plants","volume":"9","author":"Huang","year":"2022","journal-title":"Environ. Sci. Nano"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1080\/10590501.2019.1676600","article-title":"Phytotoxic effect of silver nanoparticles on seed germination and growth of terrestrial plants","volume":"37","author":"Budhani","year":"2019","journal-title":"J. Environ. Sci. Health Part C"},{"key":"ref_49","first-page":"493","article-title":"The effect of 35 nm silver nanoparticles on antagonistic and synergistic mineral elements in leaves and fruit of tomato (Lycopersicon esculentum Mill.)","volume":"5","author":"Shams","year":"2013","journal-title":"Int. J. Agric. Crop Sci."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Zuverza-Mena, N., Armendariz, R., Peralta-Videa, J.R., and Gardea-Torresdey, J.L. (2016). Effects of silver nanoparticles on radish sprouts: Root growth reduction and modifications in the nutritional value. Front. Plant Sci., 7.","DOI":"10.3389\/fpls.2016.00090"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"7552","DOI":"10.1080\/07391102.2024.2311333","article-title":"Insight into carbohydrate metabolism, protein quantification and mineral regulation in wheat (Triticum aestivum L.) by the action of green synthesized silver nanoparticles (AgNPs) against heat stress","volume":"43","author":"Iqbal","year":"2025","journal-title":"J. Biomol. Struct. Dyn."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1104\/pp.65.2.336","article-title":"Silver Uptake, Distribution, and Effect on Calcium, Phosphorus, and Sulfur Uptake","volume":"65","author":"Koontz","year":"1980","journal-title":"Plant Physiol."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Guzm\u00e1n-B\u00e1ez, G.A., Trejo-T\u00e9llez, L.I., Navarro-L\u00f3pez, D.E., Mej\u00eda-M\u00e9ndez, J.L., and G\u00f3mez-Merino, F.C. (2026). Influence of Silver Nanoparticles (AgNPs) on Vegetative Growth and Concentrations of Nutrients and Phytohormones in Tomato. Plants, 15.","DOI":"10.3390\/plants15030405"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"104670","DOI":"10.1016\/j.pestbp.2020.104670","article-title":"Antifungal and antioomycete activities and modes of action of isobenzofuranones isolated from the endophytic fungus Hypoxylon anthochroum strain Gseg1","volume":"169","year":"2020","journal-title":"Pestic. Biochem. Physiol."},{"key":"ref_55","unstructured":"Campbell, C.L., and Madden, L.V. (1990). Introduction to Plant Disease Epidemiology, John Wiley & Sons."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1093\/jee\/18.2.265a","article-title":"A method of computing the effectiveness of an insecticide","volume":"18","author":"Abbott","year":"1925","journal-title":"J. Econ. Entomol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"227","DOI":"10.18257\/raccefyn.854","article-title":"Comparison of inoculation methods of Fusarium oxysporum f. sp. lycopersici Sacc. (Snyder & Hansen), the causal agent of the vascular wilt of tomato","volume":"43","year":"2019","journal-title":"Rev. Acad. Colomb. Cienc. Exactas F\u00edsicas Nat."},{"key":"ref_58","first-page":"1","article-title":"M\u00e9todos de an\u00e1lisis de tejidos vegetales","volume":"40","author":"Sadzawka","year":"2007","journal-title":"Ser. Actas INIA"},{"key":"ref_59","unstructured":"(2010). Calidad del Agua. Determinaci\u00f3n de Elementos Seleccionados por Espectrometr\u00eda de Emisi\u00f3n \u00f3ptica de Plasma Acoplado Inductivamente (ICP-OES) (Standard No. UNE-EN ISO 11885)."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.meatsci.2009.04.016","article-title":"Application of near infrared reflectance spectroscopy to predict meat and meat products quality: A review","volume":"83","author":"Prieto","year":"2009","journal-title":"Meat Sci."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/17\/7916\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T17:27:43Z","timestamp":1788542863000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/17\/7916"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,4]]},"references-count":60,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["ijms27177916"],"URL":"https:\/\/doi.org\/10.3390\/ijms27177916","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,4]]}}}