{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T05:06:44Z","timestamp":1788930404101,"version":"build-2803163510"},"reference-count":61,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T00:00:00Z","timestamp":1788566400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"IInternational Science Programme (ISP), Uppsala University","award":["ETH:02"],"award-info":[{"award-number":["ETH:02"]}]},{"award":["ETH:02"],"award-info":[{"award-number":["ETH:02"]}],"id":[{"id":"https:\/\/ror.org\/048a87296","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Antimicrobial resistance is a growing global health challenge, highlighting the need for new bioactive compounds from medicinal plants. Vepris nobilis is traditionally used in East Africa for treating infections and respiratory disorders; however, its essential oil (EO) composition and antimicrobial mechanisms remain poorly characterized. This study investigated the chemical composition and antimicrobial activity of V. nobilis EO, along with an in silico evaluation of its major constituent, germacrene D. The EO was extracted by hydrodistillation and analyzed using gas chromatography-mass spectrometry (GC\u2013MS). Its antimicrobial activity was evaluated against 26 bacterial and 4 fungal strains using disc diffusion, broth microdilution, and MBC\/MFC (Minimum Bactericidal Concentration\/Minimum Fungicidal Concentration) assays. Germacrene D showed stronger activity than the EO, particularly against both multidrug resistant (MDR) and non-MDR Gram-negative bacterial strains (MIC = 10 \u00b5g\/mL), with bactericidal and fungicidal effect. Molecular docking of germacrene D against two clinically relevant enzymes\u2014dehydrosqualene synthase (CrtM) from Staphylococcus aureus and SWISS-modeled sterol 14-\u03b1-demethylase (CYP51) from Penicillium funiculosum\u2014suggested potential interactions with both targets, with a favorable predicted binding affinity for CrtM (\u22127.654 kcal\/mol) and for CYP51 (\u22125.898 kcal\/mol). These findings provide preliminary insights into a possible antimicrobial mechanism, although experimental validation is needed to confirm this hypothesis. ADMET analysis suggested favorable drug-like properties despite limited solubility. These findings provide scientific support for the traditional use of V. nobilis leaves in the treatment of respiratory infections and highlight germacrene D as a promising lead compound for further antimicrobial development.<\/jats:p>","DOI":"10.3390\/ijms27177927","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T12:12:25Z","timestamp":1788783145000},"page":"7927","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chemical Profiling and Antimicrobial Activity of the Leaf Essential Oil of Vepris nobilis (Delile) Mziray: In Silico Evaluation of the Major Constituent, Germacrene D"],"prefix":"10.3390","volume":"27","author":[{"given":"Biniam","family":"Paulos","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Chemistry and Pharmacognosy, School of Pharmacy, College of Health Sciences, Addis Ababa University, Addis Ababa P.O. Box 1176, Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0918-3635","authenticated-orcid":false,"given":"Mariamawit Y.","family":"Yeshak","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry and Pharmacognosy, School of Pharmacy, College of Health Sciences, Addis Ababa University, Addis Ababa P.O. Box 1176, Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3053-8106","authenticated-orcid":false,"given":"Avijit","family":"Mazumder","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Noida Institute of Engineering and Technology (Pharmacy Institute), 19 Knowledge Park II, Institutional Area, Greater Noida 201306, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5368-7510","authenticated-orcid":false,"given":"Peter","family":"Lindemann","sequence":"additional","affiliation":[{"name":"Division Pharmaceutical Biology and Pharmacology, Institute of Pharmacy, Martin-Luther-University, Halle-Wittenberg, Hoher Weg 8, 06120 Halle, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4007-6443","authenticated-orcid":false,"given":"Daniel","family":"Bisrat","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry and Pharmacognosy, School of Pharmacy, College of Health Sciences, Addis Ababa University, Addis Ababa P.O. Box 1176, Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaleab","family":"Asres","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry and Pharmacognosy, School of Pharmacy, College of Health Sciences, Addis Ababa University, Addis Ababa P.O. Box 1176, Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"103580","DOI":"10.1016\/j.micpath.2019.103580","article-title":"A comprehensive review of the antibacterial, antifungal and antiviral potential of essential oils and their chemical constituents against drug-resistant microbial pathogens","volume":"134","author":"Tariq","year":"2019","journal-title":"Microb. Pathog."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Mutlu-Ingok, A., Devecioglu, D., Dikmetas, D.N., Karbancioglu-Guler, F., and Capanoglu, E. (2020). Antibacterial, antifungal, antimycotoxigenic, and antioxidant activities of essential oils: An updated review. Molecules, 25.","DOI":"10.3390\/molecules25204711"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"100057","DOI":"10.1016\/j.engmic.2022.100057","article-title":"Synergistic antifungal mechanism of effective components from essential oil against Penicillium roqueforti","volume":"3","author":"Zhao","year":"2023","journal-title":"Eng. Microbiol."},{"key":"ref_4","first-page":"117","article-title":"Antimicrobial activity, in silico molecular docking, ADMET and DFT analysis of secondary metabolites from roots of three Ethiopian medicinal plants","volume":"14","author":"Anza","year":"2021","journal-title":"Adv. Appl. Bioinform. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"4066","DOI":"10.1021\/acs.jmedchem.5b00104","article-title":"pkCSM: Predicting small-molecule pharmacokinetic and toxicity properties using graph-based signatures","volume":"58","author":"Pires","year":"2015","journal-title":"J. Med. Chem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"113622","DOI":"10.1016\/j.jep.2020.113622","article-title":"Ethnomedicinal uses, phytochemistry, and pharmacology of the genus Vepris (Rutaceae): A review","volume":"267","author":"Ombito","year":"2021","journal-title":"J. Ethnopharmacol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.sajb.2023.03.057","article-title":"Phytochemistry of plants in the genus Vepris: A review and in silico analysis of their ADMET properties","volume":"157","author":"Ojuka","year":"2023","journal-title":"S. Afr. J. Bot."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1016\/j.phytochem.2003.09.013","article-title":"Furoquinoline alkaloids from Teclea nobilis","volume":"64","author":"Ahmad","year":"2003","journal-title":"Phytochemistry"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"166","DOI":"10.3923\/pjbs.2001.166.168","article-title":"Chemical and biological evaluation of essential oil of Teclea nobilis leaf","volume":"4","year":"2001","journal-title":"Pak. J. Biol. Sci."},{"key":"ref_10","unstructured":"Orwa, C., Mutua, A., Kindt, R., Jamnadass, R., and Simons, A. (2009). Agroforestree Database: A Tree Reference and Selection Guide, Version 4.0, World Agroforestry Centre (ICRAF). Available online: https:\/\/www.landscapealliance.org\/gtkp\/resource\/agroforestree-database\/."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"21337","DOI":"10.1073\/pnas.1010907107","article-title":"Mechanism of action and inhibition of dehydrosqualene yynthase","volume":"107","author":"Lin","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"4125","DOI":"10.1080\/14786419.2023.2277352","article-title":"Antifungal activity of citronellal against Trichophyton rubrum and its predictive mechanism of action by CYP51 inhibition through molecular docking","volume":"38","author":"Gouveia","year":"2024","journal-title":"Nat. Prod. Res."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Li, H.-Y., Yang, W.-Q., Zhou, X.-Z., Shao, F., Shen, T., Zhang, L.-M., and Zhang, J. (2022). Antibacterial and antifungal sesquiterpenoids: Chemistry, resources, and activity. Biomolecules, 12.","DOI":"10.3390\/biom12091271"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1128\/mr.59.2.201-222.1995","article-title":"Mechanisms of membrane toxicity of hydrocarbons","volume":"59","author":"Sikkema","year":"1995","journal-title":"Microbiol. Rev."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3989","DOI":"10.3390\/molecules17043989","article-title":"Essential oils in combination and their antimicrobial properties","volume":"17","author":"Juliani","year":"2012","journal-title":"Molecules"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1055\/s-2006-957742","article-title":"Composition and antimicrobial activity of the essential oil of Cistus creticus subsp. eriocephalus","volume":"63","author":"Demetzos","year":"1997","journal-title":"Planta Med."},{"key":"ref_17","first-page":"845","article-title":"Composition of the leaf essential oil of Phoebe formosana from Taiwan and its in vitro cytotoxic, antibacterial, and antifungal activities","volume":"11","author":"Su","year":"2016","journal-title":"Nat. Prod. Commun."},{"key":"ref_18","first-page":"1663","article-title":"Chemical composition, antimicrobial and antioxidant properties of the volatile oil and methanol extract of Xenophyllum poposum","volume":"7","author":"Tracanna","year":"2012","journal-title":"Nat. Prod. Commun."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Guimar\u00e3es, A.C., Meireles, L.M., Lemos, M.F., Guimar\u00e3es, M.C.C., Endringer, D.C., Fronza, M., and Scherer, R. (2019). Antibacterial activity of terpenes and terpenoids present in essential oils. Molecules, 24.","DOI":"10.3390\/molecules24132471"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Angane, M., Swift, S., Huang, K., Butts, C.A., and Quek, S.Y. (2022). Essential oils and their major components: An updated review on antimicrobial activities, mechanism of action and their potential application in the food industry. Foods, 11.","DOI":"10.3390\/foods11030464"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1146\/annurev-food-032818-121727","article-title":"Improving the efficacy of essential oils as antimicrobials in foods: Mechanisms of action","volume":"10","author":"Rao","year":"2019","journal-title":"Annu. Rev. Food Sci. Technol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"76","DOI":"10.3109\/1040841X.2013.763219","article-title":"Synergy between essential oil components and antibiotics: A review","volume":"40","author":"Langeveld","year":"2014","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Dias, K.J.S.D.O., Miranda, G.M., Bessa, J.R., Ara\u00fajo, A.C.J., Freitas, P.R., Almeida, R.S., Paulo, C.L.R., Neto, J.B.A., Coutinho, H.D.M., and Ribeiro-Filho, J. (2022). Terpenes as bacterial efflux pump inhibitors: A systematic review. Front. Pharmacol., 13.","DOI":"10.3389\/fphar.2022.953982"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Angelini, P. (2024). Plant-derived antimicrobials and their crucial role in combating antimicrobial resistance. Antibiotics, 13.","DOI":"10.3390\/antibiotics13080746"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1038\/s44259-025-00093-4","article-title":"Targeting fungal lipid synthesis for antifungal drug development and potentiation of contemporary antifungals","volume":"3","author":"Cramer","year":"2025","journal-title":"npj Antimicrob. Resist."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"8357","DOI":"10.1080\/07391102.2024.2310773","article-title":"In silico molecular docking and molecular dynamics simulation of \u03b3-elemene and caryophyllene identified from the essential oil of Kaempferia galanga L. against biofilm-forming proteins CrtM and SarA of Staphylococcus aureus","volume":"43","author":"Sharma","year":"2025","journal-title":"J. Biomol. Struct. Dyn."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"9995","DOI":"10.1039\/D1RA00359C","article-title":"Biologically guided isolation and ADMET profile of new factor Xa inhibitors from Glycyrrhiza glabra roots using in vitro and in silico approaches","volume":"11","author":"Ibrahim","year":"2021","journal-title":"RSC Adv."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1086\/381972","article-title":"Clinical relevance of bacteriostatic versus bactericidal mechanisms of action in the treatment of Gram-positive bacterial infections","volume":"38","author":"Pankey","year":"2004","journal-title":"Clin. Infect. Dis."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.jpha.2015.11.005","article-title":"Methods for in vitro evaluating antimicrobial activity: A review","volume":"6","author":"Balouiri","year":"2016","journal-title":"J. Pharm. Anal."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"361","DOI":"10.4315\/0362-028X-64.3.361","article-title":"Improved agar diffusion method for detecting residual antimicrobial agents","volume":"64","author":"Tsai","year":"2001","journal-title":"J. Food Prot."},{"key":"ref_31","unstructured":"National Committee for Clinical Laboratory Standards (NCCLS) (1999). Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline, NCCLS. NCCLS Document M26-A."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3012462","DOI":"10.1155\/2016\/3012462","article-title":"Antimicrobial properties of plant essential oils against human pathogens and their mode of action: An updated review","volume":"2016","author":"Swamy","year":"2016","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Chouhan, S., Sharma, K., and Guleria, S. (2017). Antimicrobial activity of some essential oils\u2014Present status and future perspectives. Medicines, 4.","DOI":"10.3390\/medicines4030058"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Nazzaro, F., Fratianni, F., Coppola, R., and De Feo, V. (2017). Essential oils and antifungal activity. Pharmaceuticals, 10.","DOI":"10.3390\/ph10040086"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.bcp.2016.11.019","article-title":"Antifungals","volume":"133","author":"Campoy","year":"2017","journal-title":"Biochem. Pharmacol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/S0966-842X(03)00117-3","article-title":"Antifungal agents: Mechanisms of action","volume":"11","author":"Odds","year":"2003","journal-title":"Trends Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.1126\/science.1153018","article-title":"A cholesterol biosynthesis inhibitor blocks Staphylococcus aureus virulence","volume":"319","author":"Liu","year":"2008","journal-title":"Science"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"4950","DOI":"10.1128\/IAI.00204-06","article-title":"Staphyloxanthin plays a role in the fitness of Staphylococcus aureus and its ability to cope with oxidative stress","volume":"74","author":"Clauditz","year":"2006","journal-title":"Infect. Immun."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Elmesseri, R.A., Saleh, S.E., Elsherif, H.M., Yahia, I.S., and Aboshanab, K.M. (2022). Staphyloxanthin as a potential novel target for deciphering promising anti-Staphylococcus aureus agents. Antibiotics, 11.","DOI":"10.3390\/antibiotics11030298"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.1021\/jm0306430","article-title":"Glide: A new approach for rapid, accurate docking and scoring. I. Method and assessment of docking accuracy","volume":"47","author":"Friesner","year":"2004","journal-title":"J. Med. Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1007\/s12154-014-0121-1","article-title":"Resistance to antifungals that target CYP51","volume":"7","author":"Parker","year":"2014","journal-title":"J. Chem. Biol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Salmaso, V., and Moro, S. (2018). Bridging molecular docking to molecular dynamics in exploring ligand\u2013protein recognition processes: An overview. Front. Pharmacol., 9.","DOI":"10.3389\/fphar.2018.00923"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1016\/j.fct.2007.09.106","article-title":"Biological effects of essential oils: A review","volume":"46","author":"Bakkali","year":"2008","journal-title":"Food Chem. Toxicol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"100217","DOI":"10.1016\/j.fochx.2022.100217","article-title":"Terpenes and terpenoids as the main bioactive compounds of essential oils: Their roles in human health and potential application as natural food preservatives","volume":"13","author":"Masyita","year":"2022","journal-title":"Food Chem. X"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0169-409X(00)00129-0","article-title":"Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings","volume":"46","author":"Lipinski","year":"2001","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1186\/2193-1801-2-353","article-title":"An in silico evaluation of the ADMET profile of the StreptomeDB database","volume":"2","year":"2013","journal-title":"SpringerPlus"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"6","DOI":"10.2174\/1874285801408010006","article-title":"Essential oils: A new horizon in combating bacterial antibiotic resistance","volume":"8","author":"Yap","year":"2014","journal-title":"Open Microbiol. J."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Baldim, I., Oliveira, A.M., Souto, E.B., and Oliveira, W.P. (2022). Cyclodextrins-in-liposomes: A promising delivery system for Lippia sidoides and Syzygium aromaticum essential oils. Life, 12.","DOI":"10.3390\/life12010095"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"101270","DOI":"10.1016\/j.scp.2023.101270","article-title":"Hydrodistillation-based essential oil extraction: A quest for the most effective and cleaner technology","volume":"36","author":"Katekar","year":"2023","journal-title":"Sustain. Chem. Pharm."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.jchromb.2008.04.042","article-title":"Retention index thresholds for compound matching in GC\u2013MS metabolite profiling","volume":"871","author":"Strehmel","year":"2008","journal-title":"J. Chromatogr. B"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1002\/ffj.1887","article-title":"Linear retention indices in gas chromatographic analysis: A review","volume":"23","author":"Zellner","year":"2008","journal-title":"Flavour Fragr. J."},{"key":"ref_52","unstructured":"Wiley Science Solutions (2026). Wiley Registry\/NIST EI Mass Spectral Library 2026, Wiley Science Solutions. Available online: https:\/\/sciencesolutions.wiley.com\/solutions\/technique\/gc-ms\/wiley-registry-nist-mass-spectral-library\/."},{"key":"ref_53","unstructured":"Adams, R.P. (2007). Identification of Essential Oil Components by GAS Chromatography\/Mass Spectrometry, Allured Publishing Corporation. [4th ed.]."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"S64","DOI":"10.5530\/pj.2017.6s.159","article-title":"Phytochemical screening and antimicrobial activity of rhizomes of Hedychium spicatum","volume":"9","author":"Arora","year":"2017","journal-title":"Pharmacogn. J."},{"key":"ref_55","first-page":"198","article-title":"Antimicrobial action of the leaf extract of Lagerstroemia parviflora Roxb","volume":"21","author":"Mazumder","year":"2002","journal-title":"Anc. Sci. Life"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1007\/s10822-013-9644-8","article-title":"Protein and ligand preparation: Parameters, protocols, and influence on virtual screening enrichments","volume":"27","author":"Sastry","year":"2013","journal-title":"J. Comput. Aided Mol. Des."},{"key":"ref_57","unstructured":"Schr\u00f6dinger, LLC (2025). Schr\u00f6dinger Release 2025-2, Schr\u00f6dinger, LLC."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1007\/s10822-007-9133-z","article-title":"Epik: A software program for pKa prediction and protonation-state generation for drug-like molecules","volume":"21","author":"Shelley","year":"2007","journal-title":"J. Comput. Aided Mol. Des."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"W296","DOI":"10.1093\/nar\/gky427","article-title":"SWISS-MODEL: Homology modelling of protein structures and complexes","volume":"46","author":"Waterhouse","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Studer, G., Tauriello, G., Bienert, S., Biasini, M., Johner, N., and Schwede, T. (2021). ProMod3\u2014A versatile homology modelling toolbox. PLoS Comput. Biol., 17.","DOI":"10.1371\/journal.pcbi.1008667"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1007\/978-1-60761-842-3_6","article-title":"Template-based protein structure modeling","volume":"673","author":"Fiser","year":"2010","journal-title":"Methods Mol. Biol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/17\/7927\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T04:12:49Z","timestamp":1788927169000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/17\/7927"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,5]]},"references-count":61,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["ijms27177927"],"URL":"https:\/\/doi.org\/10.3390\/ijms27177927","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,5]]}}}