{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T10:08:36Z","timestamp":1788948516614,"version":"build-2803163510"},"reference-count":51,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005196","name":"Youth Innovation of the Chinese Academy of Agricultural Sciences","doi-asserted-by":"publisher","award":["Y2023QC31"],"award-info":[{"award-number":["Y2023QC31"]}],"id":[{"id":"10.13039\/501100005196","id-type":"DOI","asserted-by":"publisher"}]},{"award":["Y2023QC31"],"award-info":[{"award-number":["Y2023QC31"]}],"id":[{"id":"https:\/\/ror.org\/0313jb750","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009620","name":"Science and Technology Project of Gansu Province","doi-asserted-by":"publisher","award":["25JRRA452"],"award-info":[{"award-number":["25JRRA452"]}],"id":[{"id":"10.13039\/501100009620","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund","award":["1610322026001"],"award-info":[{"award-number":["1610322026001"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund","award":["1610322025010"],"award-info":[{"award-number":["1610322025010"]}]},{"name":"Science and Technology Innovation Program of Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences","award":["CAAS-LMY-04"],"award-info":[{"award-number":["CAAS-LMY-04"]}]},{"award":["CAAS-LMY-04"],"award-info":[{"award-number":["CAAS-LMY-04"]}],"id":[{"id":"https:\/\/ror.org\/0313jb750","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Aquaporins (AQPs) are key channels for water and small molecule transport across membranes, and represent early targets of stress signaling pathways in plants. Salicornia europaea is a typical halophyte adapted to saline-alkaline and nutrient-poor soils. However, studies on AQPs from S. europaea and their precise roles in abiotic stress responses remain limited. In this study, SeTIP2;3 and SePIP1;4 were cloned from S. europaea. Bioinformatics analysis confirmed that they belong to the TIP and PIP subfamilies of AQPs, respectively. Subcellular localization analysis revealed that SeTIP2;3 and SePIP1;4 were localized to the tonoplast and plasma membrane, respectively. Under polyethylene glycol (PEG) treatment, SeTIP2;3 showed increased transcription levels in the roots, and SePIP1;4 showed varied degrees of increased transcription in both roots and shoots. Heterologous expression of both genes in Arabidopsis thaliana was performed to investigate their functions. Under germination assays, the overexpression (OE) lines exhibited faster germination rates and longer primary roots (1.2\u20131.4 cm) compared to the wild-type (WT) plants (1.2 cm). Under mannitol-simulated drought stress, the transgenic lines showed reduced MDA content (23.8\u201328.7 nmol\/g), increased accumulation of proline (2.5\u20133.8 mg\/g) and soluble sugars (39.4\u201349.3 mg\/g), and higher chlorophyll content (0.7\u20130.9 mg\/g) than WT plants (30.9 nmol\/g; 2.4 mg\/g; 30.6 mg\/g; 0.7 mg\/g). Furthermore, under natural drought conditions, the OE lines displayed lower water loss rates and higher survival rates. Collectively, the findings of this study provide a theoretical basis for the potential application of Salicornia aquaporins in enhancing drought tolerance.<\/jats:p>","DOI":"10.3390\/ijms27188010","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T09:24:02Z","timestamp":1788945842000},"page":"8010","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Overexpression of SeTIP2;3 and SePIP1;4 from Salicornia europaea Enhances Drought Tolerance in Arabidopsis"],"prefix":"10.3390","volume":"27","author":[{"given":"Ruixin","family":"Zhang","sequence":"first","affiliation":[{"name":"Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China"},{"name":"Lanzhou Loess Plateau Ecological Environment Key Field Scientific Observation and Experiment Station, Ministry of Agriculture and Rural Affairs, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujie","family":"Li","sequence":"additional","affiliation":[{"name":"Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China"},{"name":"Lanzhou Loess Plateau Ecological Environment Key Field Scientific Observation and Experiment Station, Ministry of Agriculture and Rural Affairs, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiashou","family":"Shen","sequence":"additional","affiliation":[{"name":"Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China"},{"name":"Lanzhou Loess Plateau Ecological Environment Key Field Scientific Observation and Experiment Station, Ministry of Agriculture and Rural Affairs, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangxin","family":"Cui","sequence":"additional","affiliation":[{"name":"Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China"},{"name":"Lanzhou Loess Plateau Ecological Environment Key Field Scientific Observation and Experiment Station, Ministry of Agriculture and Rural Affairs, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biao","family":"Song","sequence":"additional","affiliation":[{"name":"Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China"},{"name":"Lanzhou Loess Plateau Ecological Environment Key Field Scientific Observation and Experiment Station, Ministry of Agriculture and Rural Affairs, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Wu","sequence":"additional","affiliation":[{"name":"Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China"},{"name":"Lanzhou Loess Plateau Ecological Environment Key Field Scientific Observation and Experiment Station, Ministry of Agriculture and Rural Affairs, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Xu","sequence":"additional","affiliation":[{"name":"Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China"},{"name":"Lanzhou Loess Plateau Ecological Environment Key Field Scientific Observation and Experiment Station, Ministry of Agriculture and Rural Affairs, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongshan","family":"Yang","sequence":"additional","affiliation":[{"name":"Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China"},{"name":"Lanzhou Loess Plateau Ecological Environment Key Field Scientific Observation and Experiment Station, Ministry of Agriculture and Rural Affairs, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huirong","family":"Duan","sequence":"additional","affiliation":[{"name":"Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China"},{"name":"Lanzhou Loess Plateau Ecological Environment Key Field Scientific Observation and Experiment Station, Ministry of Agriculture and Rural Affairs, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1016\/j.tplants.2021.03.011","article-title":"Addressing research bottlenecks to crop productivity","volume":"26","author":"Reynolds","year":"2021","journal-title":"Trends Plant Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1600","DOI":"10.1104\/pp.113.233791","article-title":"Aquaporins: Highly regulated channels controlling plant water relations","volume":"164","author":"Chaumont","year":"2014","journal-title":"Plant Physiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1152\/physrev.00008.2015","article-title":"Aquaporins in plants","volume":"95","author":"Maurel","year":"2015","journal-title":"Physiol. 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