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J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Peripheral nerve injuries are common traumas that are difficult to overcome. Although nerve grafting can quickly recover nearly all sensitivity and function, graft availability remains an issue. Research has focused on ways to directly rebuild and promote the healing of damaged nerves using surgically implanted scaffolds. Nerve regeneration can be further promoted through strategies aimed at enhancing the regenerative microenvironment, such as improving the electrical conductivity of nerve guidance conduits. In this study, composite films of chitosan (Ch) and carboxymethyl cellulose (CMC) nanofibers were prepared. To improve the films\u2019 conductivity, reduced graphene oxide (RGO) was added. Structural analysis indicated interactions between Ch and CMC and showed that incorporation of RGO did not produce additional detectable crystalline phases. ChCMCRGO films showed slightly higher conductivity and greater surface hydrophilicity than ChCMC membranes and supported greater neurite outgrowth.<\/jats:p>","DOI":"10.3390\/ijms27188013","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T09:24:02Z","timestamp":1788945842000},"page":"8013","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Nerve Cell Responses to Biocompatible RGO\u2013Chitosan\u2013CMC Nanofiber Composite Membranes"],"prefix":"10.3390","volume":"27","author":[{"given":"Quentin","family":"Sins","sequence":"first","affiliation":[{"name":"Department of Engineering, Graduate School of Engineering, Kyushu Institute of Technology, 1-1 Sensui-cho, Tobata-ku, Kitakyushu-shi 804-8550, Fukuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Giulia","family":"Zo","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences, and Cavalieri Ottolenghi Neuroscience Institute, University of Turin, Regione Gonzole 10, 10043 Orbassano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3341-5489","authenticated-orcid":false,"given":"Giuliana","family":"Paravizzini","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences, and Cavalieri Ottolenghi Neuroscience Institute, University of Turin, Regione Gonzole 10, 10043 Orbassano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Elena Raluca","family":"Sandu","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences, and Cavalieri Ottolenghi Neuroscience Institute, University of Turin, Regione Gonzole 10, 10043 Orbassano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8907-473X","authenticated-orcid":false,"given":"Stefania","family":"Raimondo","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences, and Cavalieri Ottolenghi Neuroscience Institute, University of Turin, Regione Gonzole 10, 10043 Orbassano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"resources","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]},{"given":"Wang","family":"Meng-Jiy","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, National Taiwan University of Science and Technology, No. 43, Sec. 4, Keelung Rd., Taipei 106, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"resources","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Federica","family":"Fregnan","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences, and Cavalieri Ottolenghi Neuroscience Institute, University of Turin, Regione Gonzole 10, 10043 Orbassano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4207-4393","authenticated-orcid":false,"given":"Yuki","family":"Shirosaki","sequence":"additional","affiliation":[{"name":"Department of Materials Science, Faculty of Engineering, Kyushu Institute of Technology, 1-1 Sensui-cho, Tobata-ku, Kitakyushu-shi 804-8550, Fukuoka, Japan"},{"name":"Collaborative Research Centre for Green Materials on Environmental Technology, Kyushu Institute of Technology, 1-1 Sensui-cho, Tobata-ku, Kitakyushu-shi 804-8550, Fukuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1615\/CritRevBiomedEng.2015014015","article-title":"Peripheral Nerve Regeneration Strategies: Electrically Stimulating Polymer Based Nerve Growth Conduits","volume":"43","author":"Anderson","year":"2015","journal-title":"Crit. 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