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J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Astrocytes are extensively interconnected via gap junction channels formed primarily by connexin 43 (Cx43) and connexin 30 (Cx30), two proteins that play central roles in intercellular signaling and homeostatic regulation in the brain. Although the functional properties of these connexins have been widely investigated, their spatial organization within astrocytes and its evolution during postnatal development remain poorly characterized. Here, we combined confocal and stimulated emission depletion (STED) super-resolution microscopy to examine the expression, distribution and colocalization of Cx43 and Cx30 immunoreactive puncta in hippocampal astrocytes from postnatal day 15 to adulthood. Quantitative analyses revealed a progressive increase in the number of both Cx43 and Cx30 puncta during development, whereas puncta size remained largely unchanged. Although connexin puncta appeared more distally distributed in mature astrocytes, this shift was fully accounted for by the growth of astrocytes during development. In addition, colocalization between Cx43 and Cx30 increased during maturation, reaching a stable level after postnatal day 30. Finally, STED super-resolution imaging revealed a diversity of connexin arrangements, including isolated puncta as well as complex assemblies composed of multiple neighboring connexin clusters. Together, these findings provide a quantitative characterization of the developmental remodeling of astroglial connexins and identify structural features that may contribute to the maturation of astrocytic networks.<\/jats:p>","DOI":"10.3390\/ijms27188042","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T16:43:16Z","timestamp":1788972196000},"page":"8042","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Spatio-Temporal Expression Patterns of Connexins 43 and 30 in Hippocampal Astrocytes During Postnatal Development"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2082-9126","authenticated-orcid":false,"given":"Alejandro","family":"Uribe-Arias","sequence":"first","affiliation":[{"name":"Center for Interdisciplinary Research in Biology, Coll\u00e8ge de France, CNRS, INSERM, Universit\u00e9 PSL, PSL-Neuro, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"J\u00e9r\u00f4me","family":"Ribot","sequence":"additional","affiliation":[{"name":"Center for Interdisciplinary Research in Biology, Coll\u00e8ge de France, CNRS, INSERM, Universit\u00e9 PSL, PSL-Neuro, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"software","vocabulary":"credit"}]},{"given":"Pascal","family":"Ezan","sequence":"additional","affiliation":[{"name":"Center for Interdisciplinary Research in Biology, Coll\u00e8ge de France, CNRS, INSERM, Universit\u00e9 PSL, PSL-Neuro, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Philippe","family":"Mailly","sequence":"additional","affiliation":[{"name":"Center for Interdisciplinary Research in Biology, Coll\u00e8ge de France, CNRS, INSERM, Universit\u00e9 PSL, PSL-Neuro, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5574-888X","authenticated-orcid":false,"given":"Nathalie","family":"Rouach","sequence":"additional","affiliation":[{"name":"Center for Interdisciplinary Research in Biology, Coll\u00e8ge de France, CNRS, INSERM, Universit\u00e9 PSL, PSL-Neuro, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1038\/s41583-018-0080-6","article-title":"Versatile Control of Synaptic Circuits by Astrocytes: Where, When and How?","volume":"19","author":"Zapata","year":"2018","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1126\/science.1164022","article-title":"Astroglial Metabolic Networks Sustain Hippocampal Synaptic Transmission","volume":"322","author":"Rouach","year":"2008","journal-title":"Science"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1038\/nature06063","article-title":"Gap Junction Adhesion Is Necessary for Radial Migration in the Neocortex","volume":"448","author":"Elias","year":"2007","journal-title":"Nature"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.tins.2005.02.006","article-title":"Emerging Complexities in Identity and Function of Glial Connexins","volume":"28","author":"Theis","year":"2005","journal-title":"Trends Neurosci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1017\/S0033583501003705","article-title":"Emerging issues of connexin channels: Biophysics fills the gap","volume":"34","author":"Harris","year":"2001","journal-title":"Q. Rev. Biophys."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"18442","DOI":"10.1073\/pnas.1107386108","article-title":"Plasticity of Astroglial Networks in Olfactory Glomeruli","volume":"108","author":"Roux","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1046\/j.1460-9568.2003.02784.x","article-title":"Connexin30-Deficient Mice Show Increased Emotionality and Decreased Rearing Activity in the Open-Field along with Neurochemical Changes","volume":"18","author":"Dere","year":"2003","journal-title":"Eur. J. Neurosci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2313","DOI":"10.1046\/j.1460-9568.2003.02971.x","article-title":"Mice with Astrocyte-Directed Inactivation of Connexin43 Exhibit Increased Exploratory Behaviour, Impaired Motor Capacities, and Changes in Brain Acetylcholine Levels","volume":"18","author":"Frisch","year":"2003","journal-title":"Eur. J. Neurosci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1523\/JNEUROSCI.23-03-00766.2003","article-title":"Accelerated Hippocampal Spreading Depression and Enhanced Locomotory Activity in Mice with Astrocyte-Directed Inactivation of Connexin43","volume":"23","author":"Theis","year":"2003","journal-title":"J. Neurosci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/0165-3806(92)90077-A","article-title":"Differential Anatomical and Cellular Patterns of Connexin43 Expression during Postnatal Development of Rat Brain","volume":"66","author":"Yamamoto","year":"1992","journal-title":"Dev. Brain Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/S0306-4522(98)00191-2","article-title":"Connexin30 in Rodent, Cat and Human Brain: Selective Expression in Gray Matter Astrocytes, Co-Localization with Connexin43 at Gap Junctions and Late Developmental Appearance","volume":"88","author":"Nagy","year":"1999","journal-title":"Neuroscience"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1002\/(SICI)1098-1136(19990115)25:2<111::AID-GLIA2>3.0.CO;2-I","article-title":"Late Onset and Increasing Expression of the Gap Junction Protein Connexin30 in Adult Murine Brain and Long-Term Cultured Astrocytes","volume":"25","author":"Kunzelmann","year":"1999","journal-title":"Glia"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.devbrainres.2005.09.011","article-title":"Postnatal Development of GFAP, Connexin43 and Connexin30 in Cat Visual Cortex","volume":"160","author":"Rochefort","year":"2005","journal-title":"Dev. Brain Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1038\/nature24638","article-title":"Astrocytic Neuroligins Control Astrocyte Morphogenesis and Synaptogenesis","volume":"551","author":"Stogsdill","year":"2017","journal-title":"Nature"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"dev155275","DOI":"10.1242\/dev.155275","article-title":"Connexin 30 Controls Astroglial Polarization during Postnatal Brain Development","volume":"145","author":"Calvo","year":"2018","journal-title":"Development"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1002\/glia.20698","article-title":"Neurons Control the Expression of Connexin 30 and Connexin 43 in Mouse Cortical Astrocytes","volume":"56","author":"Koulakoff","year":"2008","journal-title":"Glia"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1915","DOI":"10.1002\/glia.24590","article-title":"Connexin 30 Locally Controls Actin Cytoskeleton and Mechanical Remodeling in Motile Astrocytes","volume":"72","author":"Ribot","year":"2024","journal-title":"Glia"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1002\/cne.903020414","article-title":"On the organization of astrocytic gap junctions in rat brain as suggested by LM and EM immunohistochemistry of connexin43 expression","volume":"302","author":"Yamamoto","year":"1990","journal-title":"J. Comp. Neurol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1016\/S0306-4522(96)00394-6","article-title":"Connexin-43 in Rat Spinal Cord: Localization in Astrocytes and Identification of Heterotypic Astro-Oligodendrocytic Gap Junctions","volume":"76","author":"Ochalski","year":"1996","journal-title":"Neuroscience"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S0165-0173(99)00066-1","article-title":"Connexins and Gap Junctions of Astrocytes and Oligodendrocytes in the CNS","volume":"32","author":"Nagy","year":"2000","journal-title":"Brain Res. Rev."},{"key":"ref_21","unstructured":"Harris, A.L., and Locke, D. (2009). Biogenesis and Degradation of Gap Junctions. Connexins: A Guide, Humana Press."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1038\/nn.3662","article-title":"Connexin 30 Sets Synaptic Strength by Controlling Astroglial Synapse Invasion","volume":"17","author":"Pannasch","year":"2014","journal-title":"Nat. Neurosci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1126\/science.abf5273","article-title":"Astrocytes Close the Mouse Critical Period for Visual Plasticity","volume":"373","author":"Ribot","year":"2021","journal-title":"Science"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1002\/glia.21120","article-title":"Role of Astroglial Connexin30 in Hippocampal Gap Junction Coupling","volume":"59","author":"Gosejacob","year":"2011","journal-title":"Glia"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1038\/jcbfm.2013.206","article-title":"Hypothalamic Astroglial Connexins Are Required for Brain Glucose Sensing-Induced Insulin Secretion","volume":"34","author":"Allard","year":"2014","journal-title":"J. Cereb. Blood Flow. Metab."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.1016\/j.neuron.2017.08.022","article-title":"Connexin 43-Mediated Astroglial Metabolic Networks Contribute to the Regulation of the Sleep-Wake Cycle","volume":"95","author":"Clasadonte","year":"2017","journal-title":"Neuron"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1093\/cercor\/bhz123","article-title":"Neuronal Activity Drives Astroglial Connexin 30 in Perisynaptic Processes and Shapes Its Functions","volume":"30","author":"Vasile","year":"2020","journal-title":"Cereb. Cortex"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"7743","DOI":"10.1523\/JNEUROSCI.0341-09.2009","article-title":"Deletion of Astrocyte Connexins 43 and 30 Leads to a Dysmyelinating Phenotype and Hippocampal CA1 Vacuolation","volume":"29","author":"Lutz","year":"2009","journal-title":"J. Neurosci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2159","DOI":"10.1002\/jnr.24026","article-title":"Probing Nano-Organization of Astroglia with Multi-Color Super-Resolution Microscopy","volume":"95","author":"Heller","year":"2017","journal-title":"J. Neurosci. Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1906","DOI":"10.1038\/s41467-020-15648-4","article-title":"Structural Basis of Astrocytic Ca2+ Signals at Tripartite Synapses","volume":"11","author":"Arizono","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1002\/glia.24103","article-title":"Deciphering the Functional Nano-Anatomy of the Tripartite Synapse Using Stimulated Emission Depletion Microscopy","volume":"70","author":"Arizono","year":"2022","journal-title":"Glia"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.bbamem.2004.01.007","article-title":"Regulation of Connexin Biosynthesis, Assembly, Gap Junction Formation, and Removal","volume":"1662","author":"Segretain","year":"2004","journal-title":"Biochim. Biophys. Acta (BBA)-Biomembr."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Solan, J.L., and Lampe, P.D. (2020). Src Regulation of Cx43 Phosphorylation and Gap Junction Turnover. Biomolecules, 10.","DOI":"10.3390\/biom10121596"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3342","DOI":"10.1091\/mbc.e09-04-0288","article-title":"Gap Junction Turnover Is Achieved by the Internalization of Small Endocytic Double-Membrane Vesicles","volume":"20","author":"Falk","year":"2009","journal-title":"Mol. Biol. Cell"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1002\/glia.10278","article-title":"Coupling of Astrocyte Connexins Cx26, Cx30, Cx43 to Oligodendrocyte Cx29, Cx32, Cx47: Implications from Normal and Connexin32 Knockout Mice","volume":"44","author":"Nagy","year":"2003","journal-title":"Glia"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"4313","DOI":"10.1523\/JNEUROSCI.3303-03.2004","article-title":"Four Classes of Intercellular Channels between Glial Cells in the CNS","volume":"24","author":"Altevogt","year":"2004","journal-title":"J. Neurosci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.brainres.2012.06.045","article-title":"Connexin-Based Intercellular Communication and Astrocyte Heterogeneity","volume":"1487","author":"Theis","year":"2012","journal-title":"Brain Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"13949","DOI":"10.1523\/JNEUROSCI.3395-07.2007","article-title":"Two Distinct Heterotypic Channels Mediate Gap Junction Coupling between Astrocyte and Oligodendrocyte Connexins","volume":"27","author":"Freidin","year":"2007","journal-title":"J. Neurosci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1002\/glia.23196","article-title":"Subcellular Reorganization and Altered Phosphorylation of the Astrocytic Gap Junction Protein Connexin43 in Human and Experimental Temporal Lobe Epilepsy","volume":"65","author":"Deshpande","year":"2017","journal-title":"Glia"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1002\/1098-1136(20010101)33:1<72::AID-GLIA1007>3.0.CO;2-A","article-title":"GFAP Promoter-Controlled EGFP-Expressing Transgenic Mice: A Tool to Visualize Astrocytes and Astrogliosis in Living Brain Tissue","volume":"33","author":"Nolte","year":"2001","journal-title":"Glia"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Rueden, C.T., Schindelin, J., Hiner, M.C., DeZonia, B.E., Walter, A.E., Arena, E.T., and Eliceiri, K.W. (2017). ImageJ2: ImageJ for the next Generation of Scientific Image Data. BMC Bioinform., 18.","DOI":"10.1186\/s12859-017-1934-z"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: An Open-Source Platform for Biological-Image Analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Reyes-Aldasoro, C.C., Janowczyk, A., Veta, M., Bankhead, P., and Sirinukunwattana, K. (2019). Bringing Open Data to Whole Slide Imaging. Proceedings of the Digital Pathology, Springer International Publishing.","DOI":"10.1007\/978-3-030-23937-4"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1840","DOI":"10.1093\/bioinformatics\/btt276","article-title":"TANGO: A Generic Tool for High-Throughput 3D Image Analysis for Studying Nuclear Organization","volume":"29","author":"Ollion","year":"2013","journal-title":"Bioinformatics"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"a020370","DOI":"10.1101\/cshperspect.a020370","article-title":"Astrocytes Control Synapse Formation, Function, and Elimination","volume":"7","author":"Chung","year":"2015","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"ref_46","first-page":"371","article-title":"Chapter Nine\u2014Role of Astrocytes in Synapse Formation and Maturation","volume":"Volume 142","author":"Bashaw","year":"2021","journal-title":"Molecular Mechanisms of Neural Development and Insights into Disease"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1893","DOI":"10.1002\/glia.23666","article-title":"Astrocyte Lineage Cells Are Essential for Functional Neuronal Differentiation and Synapse Maturation in Human iPSC-Derived Neural Networks","volume":"67","author":"Klapper","year":"2019","journal-title":"Glia"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"103092","DOI":"10.1016\/j.conb.2025.103092","article-title":"Astroglial Regulation of Critical Period Plasticity in the Developing Brain","volume":"94","author":"Ribot","year":"2025","journal-title":"Curr. Opin. Neurobiol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/18\/8042\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T04:29:24Z","timestamp":1789100964000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/18\/8042"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,9]]},"references-count":48,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["ijms27188042"],"URL":"https:\/\/doi.org\/10.3390\/ijms27188042","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,9]]}}}