{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T11:10:17Z","timestamp":1789125017941,"version":"build-2803163510"},"reference-count":23,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"2023 Shaoguan Social Development Science and Technology Collaborative Innovation System Project for Supporting Scientific Researchers","award":["230330178036357"],"award-info":[{"award-number":["230330178036357"]}]},{"DOI":"10.13039\/501100006770","name":"2023 University-level Quality Engineering Construction Project of Shaoguan University","doi-asserted-by":"publisher","award":["Shaoyuan Jiao [2024] No. 30"],"award-info":[{"award-number":["Shaoyuan Jiao [2024] No. 30"]}],"id":[{"id":"10.13039\/501100006770","id-type":"DOI","asserted-by":"publisher"}]},{"award":["Shaoyuan Jiao [2024] No. 30"],"award-info":[{"award-number":["Shaoyuan Jiao [2024] No. 30"]}],"id":[{"id":"https:\/\/ror.org\/0286g6711","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>miR-155 is an oncogenic microRNA implicated in breast cancer, but its downstream post-transcriptional targets remain incompletely defined. This study tested membrane-associated RING-CH 7 (MARCH7) as a direct miR-155 target and examined associated molecular and cellular phenotypes in MCF-7 and MDA-MB-231 breast cancer cells. Basal miR-155 expression was assessed in MCF-10A, MCF-7, and MDA-MB-231 cells. MCF-7 cells were analyzed by MTT, RT-qPCR, Western blotting, bioinformatic prediction, dual-luciferase reporter assays, and MARCH7 knockdown; MDA-MB-231 cells were additionally evaluated by MTT, RT-qPCR, wound-healing, and Transwell invasion assays. miR-155 was more abundant in the breast cancer cell lines than in MCF-10A cells. In MCF-7 cells, miR-155 overexpression increased MTT-derived metabolic viability, reduced MARCH7 and PTEN expression, increased Cyclin D1, reduced cleaved caspase-3, and was associated with increased PI3K and AKT phosphorylation. In MDA-MB-231 cells, RT-qPCR confirmed successful miR-155 modulation, whereas MTT changes were modest; these small changes may reflect either model\/endpoint differences or limited sensitivity of the MTT assay under the tested conditions. Dual-luciferase assays confirmed direct targeting of the MARCH7 3\u2032-UTR by miR-155, while miR-155 inhibition markedly reduced MDA-MB-231 migration and invasion. These findings establish MARCH7 as a direct miR-155 target in the models examined. The PTEN\/PI3K\/AKT changes are associated downstream findings and are not yet proven to be mediated specifically by MARCH7.<\/jats:p>","DOI":"10.3390\/ijms27188092","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T10:51:37Z","timestamp":1789123897000},"page":"8092","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["miR-155 Directly Targets MARCH7 and Modulates Viability and Invasive Phenotypes in Breast Cancer Cells"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1555-8459","authenticated-orcid":false,"given":"Xiaohua","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Medical Technology, School of Medicine, Shaoguan University, Shaoguan 512026, China"},{"name":"Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Pulau Pinang 11800, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"}]},{"given":"Huayun","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Nursing, School of Medicine, Shaoguan University, Shaoguan 512026, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]},{"given":"Fangying","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Pulau Pinang 11800, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Yue","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, School of Medicine, Shaoguan University, Shaoguan 512026, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Lei","family":"Rao","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, School of Medicine, Shaoguan University, Shaoguan 512026, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4972","DOI":"10.1097\/JS9.0000000000002543","article-title":"Associations of the NPAR index with breast cancer incidence and mortality based on the National Health and Nutrition Examination Survey (NHANES) 2001\u20132018: A cross-sectional study","volume":"111","author":"Su","year":"2025","journal-title":"Int. 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