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J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Neuroblastoma is a heterogeneous pediatric malignancy with marked molecular diversity and variable clinical behavior. Although current risk stratification incorporates clinical and genomic factors, additional biomarkers are needed to improve tumor characterization and advance precision oncology. The present study investigated the expression of miR-141-3p, miR-150-5p, miR-181a-5p, and miR-182-5p and their predicted target genes in pediatric neuroblastoma. Tumor samples from 58 neuroblastoma and 13 ganglioneuroma patients were compared with 41 adjacent non-tumor tissues, while an independent cohort of 17 frozen tumors (13 neuroblastomas and 4 ganglioneuroblastomas) was used for integrated miRNA\u2013target gene analyses. Diagnostic performance, functional enrichment, integrated analyses, and clinicopathological associations were evaluated. All four miRNAs were predominantly downregulated in tumors, with miR-150-5p and miR-181a-5p showing the most consistent reduction. A combined panel of miR-141-3p, miR-150-5p, and miR-181a-5p discriminated tumor from adjacent non-tumor tissues with 80.7% accuracy. miR-141-3p and miR-150-5p were associated with age at diagnosis, and miR-181a-5p was associated with overall survival. Functional enrichment implicated these miRNAs in neural crest development, stemness, neuronal differentiation, and major oncogenic pathways. Integrated analyses revealed reduced BCL2 expression in high-risk tumors and a positive correlation between miR-181a-5p and MYCN. These findings support these miRNAs as complementary molecular biomarkers and provide insights into miRNA-mediated regulatory networks in neuroblastoma.<\/jats:p>","DOI":"10.3390\/ijms27188139","type":"journal-article","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T12:09:51Z","timestamp":1789387791000},"page":"8139","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated miRNA\u2013Target Gene Analysis Reveals Complementary Molecular Biomarkers and Regulatory Networks in Pediatric Neuroblastoma"],"prefix":"10.3390","volume":"27","author":[{"given":"Karine","family":"Fiorentin","sequence":"first","affiliation":[{"name":"Instituto de Pesquisa Pel\u00e9 Pequeno Pr\u00edncipe, Curitiba 80250-060, PR, Brazil"},{"name":"Faculdades Pequeno Pr\u00edncipe, Curitiba 80230-020, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"given":"Solange S.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisa Pel\u00e9 Pequeno Pr\u00edncipe, Curitiba 80250-060, PR, Brazil"},{"name":"Faculdades Pequeno Pr\u00edncipe, Curitiba 80230-020, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayara O.","family":"Ruthes","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisa Pel\u00e9 Pequeno Pr\u00edncipe, Curitiba 80250-060, PR, Brazil"},{"name":"Faculdades Pequeno Pr\u00edncipe, Curitiba 80230-020, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daiane","family":"Rosolen","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisa Pel\u00e9 Pequeno Pr\u00edncipe, Curitiba 80250-060, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Fernanda C. B.","family":"Berti","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisa Pel\u00e9 Pequeno Pr\u00edncipe, Curitiba 80250-060, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Hadelle H.","family":"Hassmann","sequence":"additional","affiliation":[{"name":"Hospital Pequeno Pr\u00edncipe, Curitiba 80250-060, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula","family":"Kuczynski","sequence":"additional","affiliation":[{"name":"Hospital Pequeno Pr\u00edncipe, Curitiba 80250-060, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"resources","vocabulary":"credit"}]},{"given":"L\u00facia","family":"de Noronha","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Celular e Molecular e Patologia Experimental, Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1, Curitiba 80215-901, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"resources","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6446-8660","authenticated-orcid":false,"given":"Hellen G. G.","family":"Santos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Bioinform\u00e1tica e Gen\u00f4mica Cl\u00ednica, Instituto Carlos Chagas, Fiocruz, Curitiba 81350-010, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cleanderson R.","family":"Fidelis","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisa Pel\u00e9 Pequeno Pr\u00edncipe, Curitiba 80250-060, PR, Brazil"},{"name":"Faculdades Pequeno Pr\u00edncipe, Curitiba 80230-020, PR, Brazil"},{"name":"Departamento de Estat\u00edstica, Universidade Estadual da Para\u00edba, Campina Grande 58429-500, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0840-8487","authenticated-orcid":false,"given":"Aline S.","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisa Pel\u00e9 Pequeno Pr\u00edncipe, Curitiba 80250-060, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciane R.","family":"Cavalli","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisa Pel\u00e9 Pequeno Pr\u00edncipe, Curitiba 80250-060, PR, Brazil"},{"name":"Faculdades Pequeno Pr\u00edncipe, Curitiba 80230-020, PR, Brazil"},{"name":"Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC 20007, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1016\/S1470-2045(17)30186-9","article-title":"International incidence of childhood cancer, 2001\u20132010: A population-based registry study","volume":"18","author":"Colombet","year":"2017","journal-title":"Lancet Oncol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e30732","DOI":"10.1002\/pbc.30732","article-title":"A comprehensive analysis of neuroblastoma incidence, survival, and racial and ethnic disparities from 2001 to 2019","volume":"71","author":"Campbell","year":"2024","journal-title":"Pediatr. Blood Cancer"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"16078","DOI":"10.1038\/nrdp.2016.78","article-title":"Neuroblastoma","volume":"2","author":"Matthay","year":"2016","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_4","unstructured":"Instituto Nacional de C\u00e2ncer (2026). Jos\u00e9 Alencar Gomes da Silva Estimativa 2026: Incid\u00eancia de C\u00e2ncer no Brasil, INCA."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"311","DOI":"10.3109\/01913129509064236","article-title":"Comparison of Ultrastructural Features Among Neuroblastic Tumors: Maturation from Neuroblastoma to Ganglioneuroma","volume":"19","author":"Hicks","year":"1995","journal-title":"Ultrastruct. Pathol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.1038\/ng.3921","article-title":"Heterogeneity of neuroblastoma cell identity defined by transcriptional circuitries","volume":"49","author":"Boeva","year":"2017","journal-title":"Nat. Genet."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"188805","DOI":"10.1016\/j.bbcan.2022.188805","article-title":"Tumoral heterogeneity in neuroblastoma","volume":"1877","author":"Gomez","year":"2022","journal-title":"Biochim. Biophys. Acta (BBA)-Rev. Cancer"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1007\/s11912-022-01270-8","article-title":"Neuroblastoma Heterogeneity, Plasticity, and Emerging Therapies","volume":"24","author":"Lundberg","year":"2022","journal-title":"Curr. Oncol. Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2202","DOI":"10.1056\/NEJMra0804577","article-title":"Recent Advances in Neuroblastoma","volume":"362","author":"Maris","year":"2010","journal-title":"N. Engl. J. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2243","DOI":"10.1056\/NEJMoa052399","article-title":"Chromosome 1p and 11q Deletions and Outcome in Neuroblastoma","volume":"353","author":"Attiyeh","year":"2005","journal-title":"N. Engl. J. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1418","DOI":"10.1038\/bjc.2012.375","article-title":"Segmental chromosomal alterations have prognostic impact in neuroblastoma: A report from the INRG project","volume":"107","author":"Schleiermacher","year":"2012","journal-title":"Br. J. Cancer"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Chen, Y., Yang, H., Xiao, L., Yan, N., and Zhang, M. (2025). Molecular regulation and therapeutic targeting of MYCN in neuroblastoma: A comprehensive review. Front. Cell Dev. Biol., 13.","DOI":"10.3389\/fcell.2025.1683331"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.canlet.2005.01.055","article-title":"Germline mutations of the paired-like homeobox 2B (PHOX2B) gene in neuroblastoma","volume":"228","author":"Bourdeaut","year":"2005","journal-title":"Cancer Lett."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1038\/ng.3438","article-title":"TERT rearrangements are frequent in neuroblastoma and identify aggressive tumors","volume":"47","author":"Valentijn","year":"2015","journal-title":"Nat. Genet."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e247051","DOI":"10.6004\/jnccn.2024.7051","article-title":"Current and Emerging Biomarkers: Impact on Risk Stratification for Neuroblastoma","volume":"22","author":"Irwin","year":"2024","journal-title":"J. Natl. Compr. Canc. Netw."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1038\/nature07261","article-title":"Identification of ALK as a major familial neuroblastoma predisposition gene","volume":"455","author":"Laudenslager","year":"2008","journal-title":"Nature"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3229","DOI":"10.1200\/JCO.21.00278","article-title":"Revised Neuroblastoma Risk Classification System: A Report From the Children\u2019s Oncology Group","volume":"39","author":"Irwin","year":"2021","journal-title":"J. Clin. Oncol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"413","DOI":"10.6004\/jnccn.2024.0040","article-title":"Neuroblastoma, Version 2.2024, NCCN Clinical Practice Guidelines in Oncology","volume":"22","author":"Bagatell","year":"2024","journal-title":"J. Natl. Compr. Cancer Netw."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"AlKhazal, A., Chohan, S., Ross, D.J., Kim, J., and Brown, E.G. (2025). Emerging clinical and research approaches in targeted therapies for high-risk neuroblastoma. Front. Oncol., 15.","DOI":"10.3389\/fonc.2025.1553511"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Benchia, D., B\u00eec\u0103, O.D., S\u00e2rbu, I., Savu, B., Farca\u0219, D., Miron, I., Postolache, A.L., Cojocaru, E., Abbo, O., and Ciongradi, C.I. (2025). Targeting Pathways in Neuroblastoma: Advances in Treatment Strategies and Clinical Outcomes. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26104722"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1080\/14737140.2017.1364995","article-title":"Anti-GD2 immunotherapy for neuroblastoma","volume":"17","author":"Sait","year":"2017","journal-title":"Expert Rev. Anticancer Ther."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1284","DOI":"10.1056\/NEJMoa2210859","article-title":"GD2-CART01 for Relapsed or Refractory High-Risk Neuroblastoma","volume":"388","author":"Caruana","year":"2023","journal-title":"N. Engl. J. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"5523","DOI":"10.1158\/0008-5472.CAN-15-2507","article-title":"Integrative Genome-Scale Analysis Identifies Epigenetic Mechanisms of Transcriptional Deregulation in Unfavorable Neuroblastomas","volume":"76","author":"Henrich","year":"2016","journal-title":"Cancer Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1007\/s10555-020-09946-y","article-title":"Neuroblastoma and the epigenome","volume":"40","author":"Fetahu","year":"2021","journal-title":"Cancer Metastasis Rev."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1190","DOI":"10.1038\/s41417-023-00623-0","article-title":"Role of non-coding RNAs in neuroblastoma","volume":"30","author":"Anoushirvani","year":"2023","journal-title":"Cancer Gene Ther."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"22184","DOI":"10.18632\/oncotarget.25084","article-title":"Methylation of DNA and chromatin as a mechanism of oncogenesis and therapeutic target in neuroblastoma","volume":"9","author":"Schor","year":"2018","journal-title":"Oncotarget"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Epp, S., Chuah, S.M., and Halasz, M. (2023). Epigenetic Dysregulation in MYCN-Amplified Neuroblastoma. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms242317085"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1038\/nrc.2017.99","article-title":"Non-coding RNA networks in cancer","volume":"18","author":"Anastasiadou","year":"2018","journal-title":"Nat. Rev. Cancer"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1016\/j.cell.2019.10.017","article-title":"The Role of Non-coding RNAs in Oncology","volume":"179","author":"Slack","year":"2019","journal-title":"Cell"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"115527","DOI":"10.1016\/j.biopha.2023.115527","article-title":"Non-coding RNAs\u2019 function in cancer development, diagnosis and therapy","volume":"167","author":"Zhang","year":"2023","journal-title":"Biomed. Pharmacother."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1186\/s40364-022-00368-2","article-title":"The role of ncRNAs in neuroblastoma: Mechanisms, biomarkers and therapeutic targets","volume":"10","author":"Huang","year":"2022","journal-title":"Biomark. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1111\/cbdd.14263","article-title":"Targeting long noncoding RNAs in neuroblastoma: Progress and prospects","volume":"102","author":"Ataei","year":"2023","journal-title":"Chem. Biol. Drug Des."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3745","DOI":"10.1038\/s41467-024-47945-7","article-title":"A human neural crest model reveals the developmental impact of neuroblastoma-associated chromosomal aberrations","volume":"15","author":"Boswell","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1186\/s13046-022-02281-w","article-title":"Recent advances in the developmental origin of neuroblastoma: An overview","volume":"41","author":"Ponzoni","year":"2022","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0092-8674(04)00045-5","article-title":"MicroRNAs","volume":"116","author":"Bartel","year":"2004","journal-title":"Cell"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"15004","DOI":"10.1038\/sigtrans.2015.4","article-title":"The role of MicroRNAs in human cancer","volume":"1","author":"Peng","year":"2016","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.jbior.2018.12.002","article-title":"Noncoding RNA genes in cancer pathogenesis","volume":"71","author":"Pekarsky","year":"2019","journal-title":"Adv. Biol. Regul."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1515\/bmc-2017-0024","article-title":"Epigenetic regulation mechanisms of microRNA expression","volume":"8","author":"Morales","year":"2017","journal-title":"Biomol. Concepts"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Misiak, D., Hagemann, S., Bell, J.L., Busch, B., Lederer, M., Bley, N., Schulte, J.H., and H\u00fcttelmaier, S. (2021). The MicroRNA Landscape of MYCN-Amplified Neuroblastoma. Front. Oncol., 11.","DOI":"10.3389\/fonc.2021.647737"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Veeraraghavan, V.P., Jayaraman, S., Rengasamy, G., Mony, U., Ganapathy, D.M., Geetha, R.V., and Sekar, D. (2021). Deciphering the Role of MicroRNAs in Neuroblastoma. Molecules, 27.","DOI":"10.3390\/molecules27010099"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1895","DOI":"10.1007\/s11033-022-08137-y","article-title":"MicroRNAs as prospective biomarkers, therapeutic targets and pharmaceuticals in neuroblastoma","volume":"50","author":"Andreeva","year":"2023","journal-title":"Mol. Biol. Rep."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1016\/j.bbrc.2017.05.179","article-title":"miR-141-3p functions as a tumor suppressor modulating activating transcription factor 5 in glioma","volume":"490","author":"Wang","year":"2017","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"6740","DOI":"10.1038\/s41598-019-43231-5","article-title":"miR-150-5p represses TP53 tumor suppressor gene to promote proliferation of colon adenocarcinoma","volume":"9","author":"Liu","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Sugita, B.M., Rodriguez, Y., Fonseca, A.S., Nunes Souza, E., Kallakury, B., Cavalli, I.J., Ribeiro, E.M.S.F., Aneja, R., and Cavalli, L.R. (2022). MiR-150-5p Overexpression in Triple-Negative Breast Cancer Contributes to the In Vitro Aggressiveness of This Breast Cancer Subtype. Cancers, 14.","DOI":"10.3390\/cancers14092156"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"9766","DOI":"10.1080\/21655979.2021.1995102","article-title":"MicroRNA hsa-miR-150-5p inhibits nasopharyngeal carcinogenesis by suppressing PYCR1 (pyrroline-5-carboxylate reductase 1)","volume":"12","author":"Li","year":"2021","journal-title":"Bioengineered"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1016\/j.omtn.2019.04.017","article-title":"miR-150-5p Inhibits Non-Small-Cell Lung Cancer Metastasis and Recurrence by Targeting HMGA2 and \u03b2-Catenin Signaling","volume":"16","author":"Dai","year":"2019","journal-title":"Mol. Ther.\u2014Nucleic Acids"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.bbrc.2019.05.111","article-title":"MiR-150-5p regulates melanoma proliferation, invasion and metastasis via SIX1-mediated Warburg Effect","volume":"515","author":"Yang","year":"2019","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"263","DOI":"10.17219\/acem\/66842","article-title":"Role of miR-181a in the process of apoptosis of multiple malignant tumors: A literature review","volume":"27","author":"Feng","year":"2018","journal-title":"Adv. Clin. Exp. Med."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"173483","DOI":"10.1016\/j.ejphar.2020.173483","article-title":"microRNA-181a mediates the chemo-sensitivity of glioblastoma to carmustine and regulates cell proliferation, migration, and apoptosis","volume":"888","author":"Rezaei","year":"2020","journal-title":"Eur. J. Pharmacol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Souza, M.F., C\u00f3lus, I.M.S., Fonseca, A.S., Antunes, V.C., Kumar, D., and Cavalli, L.R. (2022). MiR-182-5p Modulates Prostate Cancer Aggressive Phenotypes by Targeting EMT Associated Pathways. Biomolecules, 12.","DOI":"10.3390\/biom12020187"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Suzuki, R., Amatya, V.J., Kushitani, K., Kai, Y., Kambara, T., and Takeshima, Y. (2018). miR-182 and miR-183 Promote Cell Proliferation and Invasion by Targeting FOXO1 in Mesothelioma. Front. Oncol., 8.","DOI":"10.3389\/fonc.2018.00446"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1101\/gad.257394.114","article-title":"miR-182 integrates apoptosis, growth, and differentiation programs in glioblastoma","volume":"29","author":"Kouri","year":"2015","journal-title":"Genes Dev."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"122911","DOI":"10.1016\/j.jns.2024.122911","article-title":"MiR-182 in Glioma: An insufficiently explored and controversial research area","volume":"458","author":"Chen","year":"2024","journal-title":"J. Neurol. Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2943","DOI":"10.3892\/or.2016.4640","article-title":"MicroRNA-141 and its associated gene FUS modulate proliferation, migration and cisplatin chemosensitivity in neuroblastoma cell lines","volume":"35","author":"Wang","year":"2016","journal-title":"Oncol. Rep."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1002\/mc.22839","article-title":"MiR-181a\/b induce the growth, invasion, and metastasis of neuroblastoma cells through targeting ABI1","volume":"57","author":"Liu","year":"2018","journal-title":"Mol. Carcinog."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"42274","DOI":"10.18632\/oncotarget.9786","article-title":"MicroRNA-181a suppresses parkin-mediated mitophagy and sensitizes neuroblastoma cells to mitochondrial uncoupler-induced apoptosis","volume":"7","author":"Cheng","year":"2016","journal-title":"Oncotarget"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"9027","DOI":"10.1038\/srep09027","article-title":"Genome wide expression profiling of p53 regulated miRNAs in neuroblastoma","volume":"5","author":"Rihani","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1038\/s43018-020-00171-8","article-title":"Combined inhibition of Aurora-A and ATR kinases results in regression of MYCN-amplified neuroblastoma","volume":"2","author":"Roeschert","year":"2021","journal-title":"Nat. Cancer"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1186\/s12943-022-01548-2","article-title":"Clinical significance of FBXW7 loss of function in human cancers","volume":"21","author":"Fan","year":"2022","journal-title":"Mol. Cancer"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Zhai, L., Balachandran, A., Larkin, R., Seneviratne, J.A., Chung, S.A., Lalwani, A., Tsubota, S., Beck, D., Kadomatsu, K., and Beckers, A. (2023). Mitotic Dysregulation at Tumor Initiation Creates a Therapeutic Vulnerability to Combination Anti-Mitotic and Pro-Apoptotic Agents for MYCN-Driven Neuroblastoma. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms242115571"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.1038\/s41416-023-02430-8","article-title":"Increased MCL1 dependency leads to new applications of BH3-mimetics in drug-resistant neuroblastoma","volume":"129","author":"Jacob","year":"2023","journal-title":"Br. J. Cancer"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.csbj.2015.04.003","article-title":"Identification of miRNAs contributing to neuroblastoma chemoresistance","volume":"13","author":"Ayers","year":"2015","journal-title":"Comput. Struct. Biotechnol. J."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"113636","DOI":"10.1016\/j.isci.2025.113636","article-title":"Extracellular vesicle derived miRNAs from plasma as promising diagnosis and prognosis biomarkers for neuroblastoma","volume":"28","author":"Zhou","year":"2025","journal-title":"iScience"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1136\/gutjnl-2011-301122","article-title":"miR-150 as a potential biomarker associated with prognosis and therapeutic outcome in colorectal cancer","volume":"61","author":"Ma","year":"2012","journal-title":"Gut"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"11595","DOI":"10.18632\/oncotarget.7292","article-title":"MicroRNA-150 suppresses cell proliferation and metastasis in hepatocellular carcinoma by inhibiting the GAB1-ERK axis","volume":"7","author":"Sun","year":"2016","journal-title":"Oncotarget"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Ziel-Swier, L.J.Y.M., Liu, Y., Seitz, A., De Jong, D., Koerts, J., Rutgers, B., Veenstra, R., Razak, F.R.A., Dzikiewicz-Krawczyk, A., and Van Den Berg, A. (2022). The Role of the MYC\/miR-150\/MYB\/ZDHHC11 Network in Hodgkin Lymphoma and Diffuse Large B-Cell Lymphoma. Genes, 13.","DOI":"10.3390\/genes13020227"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"5134","DOI":"10.7150\/thno.34887","article-title":"C-myc\/miR-150\/EPG5 axis mediated dysfunction of autophagy promotes development of non-small cell lung cancer","volume":"9","author":"Li","year":"2019","journal-title":"Theranostics"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"100026","DOI":"10.1016\/j.clinsp.2022.100026","article-title":"microRNA-181a-5p impedes the proliferation, migration, and invasion of retinoblastoma cells by targeting the NRAS proto-oncogene","volume":"77","author":"Ouyang","year":"2022","journal-title":"Clinics"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"108","DOI":"10.3892\/ijo.2023.5556","article-title":"Role of miR-181a-5p in cancer (Review)","volume":"63","author":"Li","year":"2023","journal-title":"Int. J. Oncol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"758","DOI":"10.3892\/ol.2020.11579","article-title":"MiR-181a functions as an oncogene by regulating CCND1 in multiple myeloma","volume":"20","author":"Yan","year":"2020","journal-title":"Oncol. Lett."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"42805","DOI":"10.18632\/oncotarget.8715","article-title":"Dysregulation and functional roles of miR-183-96-182 cluster in cancer cell proliferation, invasion and metastasis","volume":"7","author":"Ma","year":"2016","journal-title":"Oncotarget"},{"key":"ref_72","first-page":"6147","article-title":"MicroRNA-183 cluster: A promising biomarker and therapeutic target in gastrointestinal malignancies","volume":"13","author":"Zheng","year":"2023","journal-title":"Am. J. Cancer Res."},{"key":"ref_73","first-page":"2371","article-title":"miRNA-183-5p.1 promotes the migration and invasion of gastric cancer AGS cells by targeting TPM1","volume":"42","author":"Lin","year":"2019","journal-title":"Oncol. Rep."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"3447","DOI":"10.1007\/s10620-017-4755-z","article-title":"miR-182 and miR-135b Mediate the Tumorigenesis and Invasiveness of Colorectal Cancer Cells via Targeting ST6GALNAC2 and PI3K\/AKT Pathway","volume":"62","author":"Jia","year":"2017","journal-title":"Dig. Dis. Sci."},{"key":"ref_75","first-page":"1086","article-title":"MicroRNAs in neuroblastoma tumorigenesis, therapy resistance, and disease evolution","volume":"2","author":"Aravindan","year":"2019","journal-title":"Cancer Drug Resist."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.4161\/cbt.8.16.8964","article-title":"Mcl1 downregulation sensitizes neuroblastoma to cytotoxic chemotherapy and small molecule Bcl2-family antagonists","volume":"8","author":"Lestini","year":"2009","journal-title":"Cancer Biol. Ther."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"2876","DOI":"10.1080\/15384101.2019.1652033","article-title":"The crucial role of DNA-dependent protein kinase and myelin transcription factor 1-like protein in the miR-141 tumor suppressor network","volume":"18","author":"Wang","year":"2019","journal-title":"Cell Cycle"},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Sukhanova, M.V., Singatulina, A.S., Pastr\u00e9, D., and Lavrik, O.I. (2020). Fused in Sarcoma (FUS) in DNA Repair: Tango with Poly(ADP-ribose) Polymerase 1 and Compartmentalisation of Damaged DNA. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21197020"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1158\/1535-7163.MCT-20-0666","article-title":"High-Throughput Screening Identifies Idasanutlin as a Resensitizing Drug for Venetoclax-Resistant Neuroblastoma Cells","volume":"20","author":"Vernooij","year":"2021","journal-title":"Mol. Cancer Ther."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1007\/s00441-014-1981-y","article-title":"The role of microRNAs in human neural stem cells, neuronal differentiation and subtype specification","volume":"359","author":"Stappert","year":"2015","journal-title":"Cell Tissue Res."},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Perri, P., Ponzoni, M., Corrias, M.V., Ceccherini, I., Candiani, S., and Bachetti, T. (2021). A Focus on Regulatory Networks Linking MicroRNAs, Transcription Factors and Target Genes in Neuroblastoma. Cancers, 13.","DOI":"10.3390\/cancers13215528"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"dev200331","DOI":"10.1242\/dev.200331","article-title":"Linking neural crest development to neuroblastoma pathology","volume":"149","author":"Liu","year":"2022","journal-title":"Development"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1093\/neuonc\/noad034","article-title":"Modeling brain and neural crest neoplasms with human pluripotent stem cells","volume":"25","author":"Schloo","year":"2023","journal-title":"Neuro-Oncology"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1097\/MPH.0000000000000329","article-title":"PI3King the Lock: Targeting the PI3K\/Akt\/mTOR Pathway as a Novel Therapeutic Strategy in Neuroblastoma","volume":"37","author":"King","year":"2015","journal-title":"J. Pediatr. Hematol. Oncol."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1186\/s13046-021-01967-x","article-title":"A review of the biological and clinical implications of RAS-MAPK pathway alterations in neuroblastoma","volume":"40","author":"Mlakar","year":"2021","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Becker, J., and Wilting, J. (2019). WNT Signaling in Neuroblastoma. Cancers, 11.","DOI":"10.3390\/cancers11071013"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"6190","DOI":"10.1038\/onc.2017.288","article-title":"FOXO3-mediated chemo-protection in high-stage neuroblastoma depends on wild-type TP53 and SESN3","volume":"36","author":"Rupp","year":"2017","journal-title":"Oncogene"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"2549","DOI":"10.1038\/s41467-022-30237-3","article-title":"Environmental cues from neural crest derivatives act as metastatic triggers in an embryonic neuroblastoma model","volume":"13","author":"Thoinet","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"100161","DOI":"10.1016\/j.ejcped.2024.100161","article-title":"The neuroblastoma tumor microenvironment: From an in-depth characterization towards novel therapies","volume":"3","author":"Louault","year":"2024","journal-title":"EJC Paediatr. Oncol."},{"key":"ref_90","doi-asserted-by":"crossref","unstructured":"Chu, J. (2024). Validation of the biological function and prognostic significance of AURKA in neuroblastoma. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0313939"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/s12935-019-1072-y","article-title":"Silencing of AURKA augments the antitumor efficacy of the AURKA inhibitor MLN8237 on neuroblastoma cells","volume":"20","author":"Yang","year":"2020","journal-title":"Cancer Cell Int."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"1544","DOI":"10.1038\/s41416-020-0795-9","article-title":"A direct comparison of selective BH3-mimetics reveals BCL-XL, BCL-2 and MCL-1 as promising therapeutic targets in neuroblastoma","volume":"122","author":"Bierbrauer","year":"2020","journal-title":"Br. J. Cancer"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"069","DOI":"10.1055\/s-0043-1774798","article-title":"Impact of BCL-2 Expression on Course of Disease in Neuroblastoma","volume":"34","author":"Muehling","year":"2024","journal-title":"Eur. J. Pediatr. Surg."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"1466","DOI":"10.1200\/JCO.1993.11.8.1466","article-title":"Revisions of the international criteria for neuroblastoma diagnosis, staging, and response to treatment","volume":"11","author":"Brodeur","year":"1993","journal-title":"J. Clin. Oncol."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"S168","DOI":"10.21037\/gs.2019.06.01","article-title":"Neuroblastoma image-defined risk factors in adrenal neuroblastoma: Role of radiologist","volume":"8","author":"Lanza","year":"2019","journal-title":"Gland Surg."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1002\/(SICI)1097-0142(19990715)86:2<364::AID-CNCR21>3.0.CO;2-7","article-title":"The International Neuroblastoma Pathology Classification (the Shimada system)","volume":"86","author":"Shimada","year":"1999","journal-title":"Cancer"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"2451","DOI":"10.1002\/1097-0142(20011101)92:9<2451::AID-CNCR1595>3.0.CO;2-S","article-title":"International neuroblastoma pathology classification for prognostic evaluation of patients with peripheral neuroblastic tumors: A report from the Children\u2019s Cancer Group","volume":"92","author":"Shimada","year":"2001","journal-title":"Cancer"},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1200\/JCO.2008.16.6785","article-title":"The International Neuroblastoma Risk Group (INRG) Classification System: An INRG Task Force Report","volume":"27","author":"Cohn","year":"2009","journal-title":"J. Clin. Oncol."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1200\/JCO.2008.16.6876","article-title":"The International Neuroblastoma Risk Group (INRG) staging system: An INRG Task Force report","volume":"27","author":"Monclair","year":"2009","journal-title":"J. Clin. Oncol."},{"key":"ref_100","unstructured":"Rudzinski, E.R., Jarzembowski, J.A., Davis, J.L., Shimada, H., Reyes-Mugica, M., and Sebire, N. (2021). Protocol for the Examination of Resection Specimens from Patients with Neuroblastoma, College of American Pathologists."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2\u2212\u0394\u0394CT Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_102","unstructured":"GraphPad Software (GraphPad Prism, 2018). GraphPad Prism, version 8."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"D304","DOI":"10.1093\/nar\/gkad1071","article-title":"TarBase-v9.0 extends experimentally supported miRNA\u2013gene interactions to cell-types and virally encoded miRNAs","volume":"52","author":"Skoufos","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"D147","DOI":"10.1093\/nar\/gkae1072","article-title":"miRTarBase 2025: Updates to the collection of experimentally validated microRNA\u2013target interactions","volume":"53","author":"Cui","year":"2025","journal-title":"Nucleic Acids Res."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"D638","DOI":"10.1093\/nar\/gkac1000","article-title":"The STRING database in 2023: Protein-protein association networks and functional enrichment analyses for any sequenced genome of interest","volume":"51","author":"Szklarczyk","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1101\/gr.1239303","article-title":"Cytoscape: A Software Environment for Integrated Models of Biomolecular Interaction Networks","volume":"13","author":"Shannon","year":"2003","journal-title":"Genome Res."},{"key":"ref_107","unstructured":"R Core Team (2025). R: A Language and Environment for Statistical Computing [Internet], R Foundation for Statistical Computing. Available online: https:\/\/www.r-project.org\/."},{"key":"ref_108","unstructured":"Posit Team (2026). RStudio: Integrated Development Environment for R [Internet], Posit Software, PBC. Available online: https:\/\/posit.co\/."},{"key":"ref_109","doi-asserted-by":"crossref","unstructured":"Li, J., Smyth, P., Flavin, R., Cahill, S., Denning, K., Aherne, S., Guenther, S.M., O\u2019Leary, J.J., and Sheils, O. (2007). Comparison of miRNA expression patterns using total RNA extracted from matched samples of formalin-fixed paraffin-embedded (FFPE) cells and snap frozen cells. BMC Biotechnol., 7.","DOI":"10.1186\/1472-6750-7-36"},{"key":"ref_110","doi-asserted-by":"crossref","unstructured":"Vojtechova, Z., Zavadil, J., Klozar, J., Grega, M., and Tachezy, R. (2017). Comparison of the miRNA expression profiles in fresh frozen and formalin-fixed paraffin-embedded tonsillar tumors. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0179645"},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1080\/01621459.1958.10501452","article-title":"Nonparametric Estimation from Incomplete Observations","volume":"53","author":"Kaplan","year":"1958","journal-title":"J. Am. Stat. Assoc."},{"key":"ref_112","first-page":"163","article-title":"Evaluation of survival data and two new rank order statistics arising in its consideration","volume":"50","author":"Mantel","year":"1966","journal-title":"Cancer Chemother. Rep."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"2233","DOI":"10.1002\/sim.4780132105","article-title":"Cox regression analysis of multivariate failure time data: The marginal approach","volume":"13","author":"Lin","year":"1994","journal-title":"Stat. Med."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/18\/8139\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:52:28Z","timestamp":1790859148000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/18\/8139"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,12]]},"references-count":113,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["ijms27188139"],"URL":"https:\/\/doi.org\/10.3390\/ijms27188139","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,12]]}}}