{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T09:29:09Z","timestamp":1789464549508,"version":"build-2803163510"},"reference-count":32,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100015397","name":"Inner Mongolia Natural Science Foundation","doi-asserted-by":"publisher","award":["2026MS1020"],"award-info":[{"award-number":["2026MS1020"]}],"id":[{"id":"10.13039\/501100015397","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Inner Mongolia Science and Technology Program","award":["2023YFHH0056"],"award-info":[{"award-number":["2023YFHH0056"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>This study aimed to screen genes associated with drought adaptation in Cerasus humilis and analyze their sequence characteristics and evolutionary relationships. The results could provide a theoretical basis for exploring the molecular basis underlying drought adaptation of Cerasus humilis. Two-year-old wild Cerasus humilis plants were cultivated in a greenhouse, and irrigation was completely withheld for 20 consecutive days. Leaves were sampled and a cDNA library of wild Cerasus humilis was constructed. By utilizing high-throughput screening in yeast combined with mannitol-induced simulated drought stress, genes associated with drought adaptation were isolated and identified. Additionally, core candidate genes were cloned and subjected to systematic bioinformatic analysis. The results showed that: (1) the drought adaptation-associated genes ChU40rps27a and ChTp258 were obtained through high-throughput screening of yeast and mannitol-simulated drought resistance test; (2) gene cloning revealed that the open reading frame of ChU40rps27a is 471 bp long, encoding 157 amino acids, and the open reading frame of ChTp258 is 231 bp long, encoding 77 amino acids; (3) multiple sequence alignment of the genes indicated that ChU40rps27a shares 88.5% identity with apple 40S (XM_050288755), and ChTp258 shares 94.4% identity with peach (XM_020567676) in terms of nucleotide sequence; (4) the phylogenetic tree analysis revealed that ChU40rps27a is closely related to Citrus sinensisU40rps27a (XM_006487968); and ChTp258 is closely related to peach (Prunus persica, XM_020567676), apricot (Prunus dulcis, XM_034370534), plum (Prunus mume, XM_008244122), and sweet cherry (Prunus avium, XM_021951344) in the Rosaceae family, clustering in the same clade. This study identified two candidate genes involved in long-term drought adaptation rather than short-term stress responses. These findings clarify their sequence characteristics, sequence conservation and evolutionary status, providing important gene resources and theoretical support for further exploring drought adaptation related molecular processes in Cerasus humilis and developing drought-adapted molecular breeding.<\/jats:p>","DOI":"10.3390\/ijms27188186","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T08:16:48Z","timestamp":1789460208000},"page":"8186","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Screening and Bioinformatic Analysis of Drought-Adaptive-Response Genes in Wild Cerasus humilis"],"prefix":"10.3390","volume":"27","author":[{"given":"Xu","family":"Wang","sequence":"first","affiliation":[{"name":"College of Mechanical and Electrical Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenzhe","family":"Feng","sequence":"additional","affiliation":[{"name":"College of Horticultural and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"conceptualization","vocabulary":"credit"}]},{"given":"Li","family":"Fan","sequence":"additional","affiliation":[{"name":"College of Horticultural and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"}]},{"given":"Yuedong","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Horticultural and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Xiaoyan","family":"Li","sequence":"additional","affiliation":[{"name":"College of Horticultural and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"resources","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]},{"given":"Haiqing","family":"Tian","sequence":"additional","affiliation":[{"name":"College of Mechanical and Electrical Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"ref_1","first-page":"56","article-title":"Overview of the current research status of Chinese calcium fruit","volume":"37","author":"Hu","year":"2021","journal-title":"Chin. Agric. Sci. Bull."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/s12953-017-0117-1","article-title":"Water deficit mechanisms in perennial shrubs Cerasus humilis leaves revealed by physiological and proteomic analyses","volume":"15","author":"Yin","year":"2017","journal-title":"Proteome Sci."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Zhang, L., Zhang, J., and Guo, J. (2024). Relationships Between Organic Acid Metabolism and the Accumulation of Sugars and Calcium in Fruits of Cerasus humilis During Different Development Stages. Plants, 13.","DOI":"10.3390\/plants13213053"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"5479565","DOI":"10.1155\/2018\/5479565","article-title":"Polyphenol Content, Physicochemical Properties, Enzymatic Activity, Anthocyanin Profiles, and Antioxidant Capacity of Cerasus humilis (Bge.) Sok. Genotypes","volume":"2018","author":"Liu","year":"2018","journal-title":"J. Food Qual."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"137195","DOI":"10.1016\/j.ijbiomac.2024.137195","article-title":"ChBBX6 and ChBBX18 are positive regulators of anthocyanins biosynthesis and carotenoids degradation in Cerasus humilis","volume":"282","author":"Hu","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"02005","DOI":"10.1051\/e3sconf\/202018902005","article-title":"Comparative nutritional analysis for different varieties of Chinese dwarf cherry (Cerasus humilis (Bge.) Sok)","volume":"189","author":"Liang","year":"2020","journal-title":"E3S Web Conf."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e14350","DOI":"10.1111\/ppl.14350","article-title":"The key pathways for drought tolerance in Cerasus humilis were unveiled through transcriptome analysis","volume":"176","author":"Lang","year":"2024","journal-title":"Physiol. Plant."},{"key":"ref_8","first-page":"1159","article-title":"Effects of drought stress on phenotypic plasticity of Prunus avium seedlings","volume":"39","author":"Na","year":"2019","journal-title":"Guangxi Bot."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Liu, Y., Zhao, C., Tang, X., Wang, L., and Guo, R. (2024). Transcriptomic and Metabolomic Insights into ABA-Related Genes in Cerasus humilis under Drought Stress. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25147635"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"108696","DOI":"10.1016\/j.plaphy.2024.108696","article-title":"Regulatory mechanisms used by ZmMYB39 to enhance drought tolerance in maize (Zea mays) seedlings","volume":"211","author":"Ren","year":"2024","journal-title":"Plant Physiol. Biochem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1296","DOI":"10.1104\/pp.18.00502","article-title":"MdMYB88 and MdMYB124 Enhance Drought Tolerance by Modulating Root Vessels and Cell Walls in Apple","volume":"178","author":"Geng","year":"2018","journal-title":"Plant Physiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"104117","DOI":"10.1016\/j.envexpbot.2020.104117","article-title":"PtrWRKY75 overexpression reduces stomatal aperture and improves drought tolerance by salicylic acid-induced reactive oxygen species accumulation in poplar","volume":"176","author":"Zhang","year":"2020","journal-title":"Environ. Exp. Bot."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Han, H., Mu, X., Wang, P., Wang, Z., Fu, H., Gao, Y.G., and Du, J. (2021). Identification of LecRLK gene family in Cerasus humilis through genomic-transcriptomic data mining and expression analyses. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0254535"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Liu, W., Ren, W., Liu, X., He, L., Qin, C., Wang, P., Kong, L., Li, Y., Liu, Y., and Ma, W. (2023). Identification and characterization of Dof genes in Cerasus humilis. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1152685"},{"key":"ref_15","first-page":"75","article-title":"Construction and evaluation of yeast two-hybrid cDNA library of tomato","volume":"46","author":"Que","year":"2021","journal-title":"J. Southwest China Norm. Univ. (Nat. Sci. Ed.)"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"122676","DOI":"10.1016\/j.indcrop.2026.122676","article-title":"Genome-wide analysis of the sugarcane PTP family combined with yeast cDNA library screening identifies key cold-responsive genes","volume":"240","author":"Wang","year":"2026","journal-title":"Ind. Crops Prod."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Zan, L., Liu, H., Zhao, X., Du, D., and Li, K. (2024). Construction of Shoot Apical Meristem cDNA Yeast Library of Brassica napus L. and Screening of Proteins That Interact with the Inflorescence Regulatory Factors BnTFL1s. Curr. Issues Mol. Biol., 47.","DOI":"10.3390\/cimb47010015"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"105500","DOI":"10.1016\/j.dci.2025.105500","article-title":"Screening of host proteins interacting with the major capsid protein of decapod iridescent virus 1 in Litopenaeus vannamei by the yeast two-hybrid system","volume":"172","author":"Lu","year":"2025","journal-title":"Dev. Comp. Immunol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Feng, C., Guo, X., Gu, T., Hua, Y., Zhuang, X., and Zhang, K. (2023). Generation of a Triple-Shuttling Vector and the Application in Plant Plus-Strand RNA Virus Infectious cDNA Clone Construction. Int. J. Mol. Sci., 24.","DOI":"10.2139\/ssrn.4308184"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Wongsurawat, T., Jenjaroenpun, P., Wanchai, V., and Nookaew, I. (2022). Native RNA or cDNA Sequencing for Transcriptomic Analysis: A Case Study on Saccharomyces cerevisiae. Front. Bioeng. Biotechnol., 10.","DOI":"10.3389\/fbioe.2022.842299"},{"key":"ref_21","first-page":"1","article-title":"Research progress on construction strategies and analysis of cDNA library","volume":"21","author":"Yan","year":"2006","journal-title":"J. Yunnan Agric. Univ."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1073\/pnas.72.1.11","article-title":"Isolation of polypeptide thathaslym-phocyte-differentiation properties and is probably represented universally in living cells","volume":"72","author":"Goldstein","year":"1975","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Jia, W., Liu, G., Zhang, P., Li, H., Peng, Z., Wang, Y., Jemri\u0107, T., and Fu, D. (2023). The Ubiquitin\u201326S Proteasome Pathway and Its Role in the Ripening of Fleshy Fruits. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24032750"},{"key":"ref_24","first-page":"3266","article-title":"Cloning and expression analysis of polyubiquitin gene TkUBQ6 in Taraxacum kok-saghyz","volume":"19","author":"Wang","year":"2021","journal-title":"Mol. Plant Breed."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.plaphy.2013.11.003","article-title":"Manipulation of monoubiquitin improves chilling tolerance in transgenic tobacco (Nicotiana tabacum)","volume":"75","author":"Feng","year":"2014","journal-title":"Plant Physiol. Biochem."},{"key":"ref_26","first-page":"218","article-title":"Construction of cDNA library of Bipolaris sorokiniana and screening of proteins interacting with BsTup1","volume":"58","author":"Li","year":"2024","journal-title":"J. Henan Agric. Univ."},{"key":"ref_27","unstructured":"Zhang, M.Y. (2023). Identification of WRKY Transcription Factor Family and Drought Resistance Mechanism of ChWRKY34 Gene in Cerasus humilis. [Master\u2019s Thesis, Northeast Forestry University]."},{"key":"ref_28","first-page":"51","article-title":"Construction and identification of yeast cDNA library of Agrostis stolonifera under high temperature stress","volume":"45","author":"Liu","year":"2022","journal-title":"J. Hebei Agric. Univ."},{"key":"ref_29","first-page":"156","article-title":"Construction of full-length cDNA library from Cerasus humilis leaves and sequence analysis of partial clones","volume":"18","author":"Gao","year":"2010","journal-title":"J. Agric. Biotechnol."},{"key":"ref_30","first-page":"120","article-title":"Construction of yeast hybrid cDNA library during dormancy release of peony seeds","volume":"45","author":"Fei","year":"2023","journal-title":"J. Beijing For. Univ."},{"key":"ref_31","first-page":"184","article-title":"Construction and quality evaluation of wheat cDNA library induced by Puccinia striiformis","volume":"7","author":"Cheng","year":"2009","journal-title":"Mol. Plant Breed."},{"key":"ref_32","first-page":"245","article-title":"Construction of yeast two-hybrid cDNA library of rubber tree cambium tissue and screening of HbHDA6 interacting proteins","volume":"44","author":"Zhang","year":"2024","journal-title":"Guihaia"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/18\/8186\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T08:18:55Z","timestamp":1789460335000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/18\/8186"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,15]]},"references-count":32,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["ijms27188186"],"URL":"https:\/\/doi.org\/10.3390\/ijms27188186","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,15]]}}}