{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T09:02:50Z","timestamp":1789549370374,"version":"build-2803163510"},"reference-count":143,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Italian Ministry of University","award":["PNC0000001"],"award-info":[{"award-number":["PNC0000001"]}]},{"name":"Italian Ministry for University and Research (MUR)\u2014Departments of Excellence Grant"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Diabetes mellitus (DM) is a rapidly expanding global health burden and a leading cause of chronic complications. Among these, diabetic peripheral neuropathy (DN) is one of the most prevalent and disabling, affecting up to half of individuals with long-standing diabetes and involving both somatic and autonomic nerve fibers. DN is characterized by an insidious onset: axonal and glial damage begins during subclinical phases and is frequently detected only when irreversible injury has occurred, as conventional neurological examinations and nerve conduction studies lack sensitivity for detecting early-stage disease. DN pathogenesis is multifactorial and primarily driven by chronic hyperglycemia, which triggers a network of converging pathogenic mechanisms. This narrative review aims to explore the evolving landscape of DN, with a particular focus on the study of neuronal injury biomarkers in the early detection of diabetic nerve damage. In addition, it highlights the relevance of in vitro neuronal and glial models as complementary experimental platforms to investigate the molecular and cellular mechanisms underlying DN and to support the identification and validation of candidate biomarkers. Moreover, these in vitro systems provide a strategic tool for examining the impact of circulating factors on axonal and glial integrity, thereby facilitating the discovery and preliminary assessment of potential blood-based biomarkers.<\/jats:p>","DOI":"10.3390\/ijms27188234","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T07:58:59Z","timestamp":1789545539000},"page":"8234","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Modeling Diabetic Neuropathy: Injury Biomarkers for Early Detection Through Neuronal and Glial In Vitro Models"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4881-3313","authenticated-orcid":false,"given":"Luisa","family":"Muratori","sequence":"first","affiliation":[{"name":"Department of Clinical and Biological Sciences, University of Torino, 10043 Orbassano, Italy"},{"name":"Neuroscience Institute Cavalieri Ottolenghi (NICO), University of Torino, 10043 Orbassano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6967-0879","authenticated-orcid":false,"given":"Simona","family":"Rando","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences, University of Torino, 10043 Orbassano, Italy"},{"name":"Neuroscience Institute Cavalieri Ottolenghi (NICO), University of Torino, 10043 Orbassano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8907-473X","authenticated-orcid":false,"given":"Stefania","family":"Raimondo","sequence":"additional","affiliation":[{"name":"Department of Clinical and Biological Sciences, University of Torino, 10043 Orbassano, Italy"},{"name":"Neuroscience Institute Cavalieri Ottolenghi (NICO), University of Torino, 10043 Orbassano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1016\/j.clinthera.2021.04.003","article-title":"Corneal Confocal Microscopy: A Biomarker for Diabetic Peripheral Neuropathy","volume":"43","author":"Petropoulos","year":"2021","journal-title":"Clin. Ther."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1038\/s41574-021-00496-z","article-title":"Pathogenesis, diagnosis and clinical management of diabetic sensorimotor peripheral neuropathy","volume":"17","author":"Sloan","year":"2021","journal-title":"Nat. Rev. Endocrinol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"136","DOI":"10.2337\/dc16-2042","article-title":"Diabetic Neuropathy: A Position Statement by the American Diabetes Association","volume":"40","author":"Boulton","year":"2017","journal-title":"Diabetes Care"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2455","DOI":"10.1007\/s13300-024-01658-8","article-title":"Current Perspectives in Pre- and Diabetic Peripheral Neuropathy Diagnosis and Management: An Expert Statement for the Gulf Region","volume":"15","author":"Beshyah","year":"2024","journal-title":"Diabetes Ther."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2172","DOI":"10.1001\/jama.2015.13611","article-title":"Distal Symmetric Polyneuropathy: A Review","volume":"314","author":"Callaghan","year":"2015","journal-title":"JAMA"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2285","DOI":"10.2337\/dc10-1303","article-title":"Diabetic neuropathies: Update on definitions, diagnostic criteria, estimation of severity, and treatments","volume":"33","author":"Tesfaye","year":"2010","journal-title":"Diabetes Care"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"17","DOI":"10.4239\/wjd.v5.i1.17","article-title":"Cardiac autonomic neuropathy in patients with diabetes mellitus","volume":"5","author":"Dimitropoulos","year":"2014","journal-title":"World J. Diabetes"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1318","DOI":"10.1210\/er.2018-00161","article-title":"Diabetic Gastroparesis","volume":"40","author":"Bharucha","year":"2019","journal-title":"Endocr. Rev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1553","DOI":"10.2337\/diacare.26.5.1553","article-title":"Diabetic autonomic neuropathy","volume":"26","author":"Vinik","year":"2003","journal-title":"Diabetes Care"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Zhou, Q., Yang, L., Wang, Q., Li, Y., Wei, C., and Xie, L. (2022). Mechanistic investigations of diabetic ocular surface diseases. Front. Endocrinol., 13.","DOI":"10.3389\/fendo.2022.1079541"},{"key":"ref_11","first-page":"1","article-title":"Understanding Diabetic Neuropathy: Focus on Oxidative Stress","volume":"2020","author":"Pang","year":"2020","journal-title":"Oxidative Med. Cell Longev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/s11892-016-0727-5","article-title":"Inflammation as a Therapeutic Target for Diabetic Neuropathies","volume":"16","author":"Ang","year":"2016","journal-title":"Curr. Diab. Rep."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2581880","DOI":"10.1080\/21688370.2025.2581880","article-title":"Involvement of cellular and enzymatic aspects in the complexity of diabetic neuropathy","volume":"14","author":"Gupta","year":"2025","journal-title":"Tissue Barriers"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"598","DOI":"10.4103\/1673-5374.380872","article-title":"Cell metabolism pathways involved in the pathophysiological changes of diabetic peripheral neuropathy","volume":"19","author":"Lv","year":"2024","journal-title":"Neural Regen. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1016\/j.bcp.2012.01.015","article-title":"Interplay of sorbitol pathway of glucose metabolism, 12\/15-lipoxygenase, and mitogen-activated protein kinases in the pathogenesis of diabetic peripheral neuropathy","volume":"83","author":"Stavniichuk","year":"2012","journal-title":"Biochem. Pharmacol."},{"key":"ref_16","first-page":"147","article-title":"Biochemical Alterations in Diabetic Neuropathy","volume":"8","author":"Venugopal","year":"2014","journal-title":"J. Mol. Genet. Med."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Bhattacharya, R., Alam, M.R., Kamal, M.A., Seo, K.J., and Singh, L.R. (2023). AGE-RAGE axis culminates into multiple pathogenic processes: A central road to neurodegeneration. Front. Mol. Neurosci., 16.","DOI":"10.3389\/fnmol.2023.1155175"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vph.2015.06.011","article-title":"Soluble RAGEs\u2014Prospects for treating & tracking metabolic and inflammatory disease","volume":"72","author":"Schmidt","year":"2015","journal-title":"Vasc. Pharmacol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2251","DOI":"10.1007\/s00125-009-1458-9","article-title":"Multiple levels of regulation determine the role of the receptor for AGE (RAGE) as common soil in inflammation, immune responses and diabetes mellitus and its complications","volume":"52","author":"Bierhaus","year":"2009","journal-title":"Diabetologia"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"143","DOI":"10.3233\/CH-2010-1292","article-title":"The different isoforms of the receptor for advanced glycation end products are modulated by pharmacological agents","volume":"45","author":"Grossin","year":"2010","journal-title":"Clin. Hemorheol. Microcirc."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e42748","DOI":"10.1097\/MD.0000000000042748","article-title":"Mitochondrial dynamics in diabetic peripheral neuropathy: Pathogenesis, progression, and therapeutic approaches","volume":"104","author":"Yu","year":"2025","journal-title":"Medicine"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Zhang, Z., Huang, Q., Zhao, D., Lian, F., Li, X., and Qi, W. (2023). The impact of oxidative stress-induced mitochondrial dysfunction on diabetic microvascular complications. Front. Endocrinol., 14.","DOI":"10.3389\/fendo.2023.1112363"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"135316","DOI":"10.1016\/j.neulet.2020.135316","article-title":"Mechanisms of small nerve fiber pathology","volume":"737","author":"Gross","year":"2020","journal-title":"Neurosci. Lett."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"105952","DOI":"10.1016\/j.nbd.2022.105952","article-title":"Schwann cell functions in peripheral nerve development and repair","volume":"176","author":"Fledrich","year":"2023","journal-title":"Neurobiol. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1016\/j.conb.2013.06.010","article-title":"Signals regulating myelination in peripheral nerves and the Schwann cell response to injury","volume":"23","author":"Glenn","year":"2013","journal-title":"Curr. Opin. Neurobiol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Vallejo, F.A., Diaz, A., Errante, E.L., Smartz, T., Khan, A., Silvera, R., Brooks, A.E., Lee, Y.-S., Burks, S.S., and Levi, A.D. (2022). Systematic review of the therapeutic use of Schwann cells in the repair of peripheral nerve injuries: Advancements from animal studies to clinical trials. Front. Cell. Neurosci., 16.","DOI":"10.3389\/fncel.2022.929593"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Eckersley, L. (2002). Role of the Schwann Cell in Diabetic Neuropathy, Elsevier.","DOI":"10.1016\/S0074-7742(02)50081-7"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Abd Razak, N.H., Idris, J., Hassan, N.H., Zaini, F., Muhamad, N., and Daud, M.F. (2024). Unveiling the Role of Schwann Cell Plasticity in the Pathogenesis of Diabetic Peripheral Neuropathy. Int. J. Mol. Sci., 25.","DOI":"10.20944\/preprints202408.1297.v1"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Razak, N.H.A., Vijakumaran, U., Idris, I.M., Idris, J., Hassan, N.H., Zaini, F., Muhamad, N., and Daud, M.F. (2025). Hyperglycemia Modulates mTOR Signaling and Myelin Protein Expression in Schwann Cells. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26199724"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e32653","DOI":"10.1097\/MD.0000000000032653","article-title":"Mechanism of Schwann cells in diabetic peripheral neuropathy: A review","volume":"102","author":"Li","year":"2023","journal-title":"Medicine"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1727","DOI":"10.1007\/s00125-004-1525-1","article-title":"Hyperglycaemia-induced superoxide production decreases eNOS expression via AP-1 activation in aortic endothelial cells","volume":"47","author":"Srinivasan","year":"2004","journal-title":"Diabetologia"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/0014-4886(95)90023-3","article-title":"Impaired vasoreactivity to nitric oxide in experimental diabetic neuropathy","volume":"132","author":"Kirara","year":"1995","journal-title":"Exp. Neurol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.1016\/S0022-3565(25)20777-7","article-title":"Effects of a nonpeptide endothelin-1 ETA antagonist on neurovascular function in diabetic rats: Interaction with the renin-angiotensin system","volume":"278","author":"Cameron","year":"1996","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.2147\/VHRM.S3920","article-title":"The importance of endothelin-1 for microvascular dysfunction in diabetes","volume":"4","author":"Kalani","year":"2008","journal-title":"Vasc. Health Risk Manag."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"107613","DOI":"10.1016\/j.jdiacomp.2020.107613","article-title":"Molecular complexities underlying the vascular complications of diabetes mellitus\u2014A comprehensive review","volume":"34","author":"Paul","year":"2020","journal-title":"J. Diabetes Complicat."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e14477","DOI":"10.1111\/cns.14477","article-title":"How inflammation dictates diabetic peripheral neuropathy: An enlightening review","volume":"30","author":"Cheng","year":"2024","journal-title":"CNS Neurosci. Ther."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"370","DOI":"10.3390\/neurolint16020027","article-title":"Hyperglycaemia Aggravates Oxidised Low-Density Lipoprotein-Induced Schwann Cell Death via Hyperactivation of Toll-like Receptor 4","volume":"16","author":"Nihei","year":"2024","journal-title":"Neurol. Int."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/S0304-3940(01)01696-2","article-title":"Perineurium inflammation and altered connexin isoform expression in a rat model of diabetes related peripheral neuropathy","volume":"303","author":"Pitre","year":"2001","journal-title":"Neurosci. Lett."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Richner, M., Ferreira, N., Dudele, A., Jensen, T.S., Vaegter, C.B., and Gon\u00e7alves, N.P. (2019). Functional and Structural Changes of the Blood-Nerve-Barrier in Diabetic Neuropathy. Front. Neurosci., 12.","DOI":"10.3389\/fnins.2018.01038"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"674987","DOI":"10.1155\/2014\/674987","article-title":"Neuroinflammation and Oxidative Stress in Diabetic Neuropathy: Futuristic Strategies Based on These Targets","volume":"2014","author":"Sandireddy","year":"2014","journal-title":"Int. J. Endocrinol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Gardiner, N.J., and Freeman, O.J. (2016). Can Diabetic Neuropathy Be Modeled In Vitro?. International Review of Neurobiology, Elsevier.","DOI":"10.1016\/bs.irn.2016.02.004"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1974","DOI":"10.2337\/diacare.27.8.1974","article-title":"Intraepidermal Nerve Fibers Are Indicators of Small-Fiber Neuropathy in Both Diabetic and Nondiabetic Patients","volume":"27","author":"Pittenger","year":"2004","journal-title":"Diabetes Care"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.npep.2016.06.001","article-title":"Interaction of calcitonin gene related peptide (CGRP) and substance P (SP) in human skin","volume":"59","author":"Schlereth","year":"2016","journal-title":"Neuropeptides"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1055\/s-2000-7738","article-title":"Serum levels of substance P are decreased in patients with type 1 diabetes","volume":"108","author":"Kunt","year":"2000","journal-title":"Exp. Clin. Endocrinol. Diabetes"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1016\/j.jtos.2019.08.010","article-title":"Tear film substance P: A potential biomarker for diabetic peripheral neuropathy","volume":"17","author":"Tummanapalli","year":"2019","journal-title":"Ocul. Surf."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2508","DOI":"10.1002\/acn3.51234","article-title":"Blood neurofilament light: A critical review of its application to neurologic disease","volume":"7","author":"Barro","year":"2020","journal-title":"Ann. Clin. Transl. Neurol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.expneurol.2018.06.005","article-title":"Neurofilament light chain as disease biomarker in a rodent model of chemotherapy induced peripheral neuropathy","volume":"307","author":"Meregalli","year":"2018","journal-title":"Exp. Neurol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1007\/s00125-022-05846-8","article-title":"Serum neurofilament light chain: A novel biomarker for early diabetic sensorimotor polyneuropathy","volume":"66","author":"Maalmi","year":"2023","journal-title":"Diabetologia"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1002\/jum.16393","article-title":"Evaluation of Serum Neurofilament Light Chain and Nerve Ultrasound in Diabetic Neuropathy","volume":"43","author":"Cartwright","year":"2024","journal-title":"J. Ultrasound Med."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"15612","DOI":"10.1038\/s41598-024-66444-9","article-title":"The limited role of serum neurofilament light chain in predicting pain severity of patients with diabetic polyneuropathy","volume":"14","author":"Kim","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"e70030","DOI":"10.1111\/ejn.70030","article-title":"Peripherin, A New Promising Biomarker in Neurological Disorders","volume":"61","author":"Manco","year":"2025","journal-title":"Eur. J. Neurosci."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Johansen, N.J., Frugier, T., Hunne, B., and Brock, J.A. (2014). Increased peripherin in sympathetic axons innervating plantar metatarsal arteries in STZ-induced type I diabetic rats. Front. Neurosci., 8.","DOI":"10.3389\/fnins.2014.00099"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"17448069251328521","DOI":"10.1177\/17448069251328521","article-title":"Loss and damage in large-diameter sensory neurons in the db\/db diabetic mouse","volume":"21","author":"Filfilan","year":"2025","journal-title":"Mol. Pain"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Jessen, K.R., and Mirsky, R. (2019). The Success and Failure of the Schwann Cell Response to Nerve Injury. Front. Cell. Neurosci., 13.","DOI":"10.3389\/fncel.2019.00033"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"739","DOI":"10.4103\/1673-5374.206638","article-title":"Chronically denervated distal nerve stump inhibits peripheral nerve regeneration","volume":"12","author":"Ronchi","year":"2017","journal-title":"Neural Regen. Res."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"2367","DOI":"10.1111\/jcmm.12406","article-title":"Satellite glial cells in dorsal root ganglia are activated in streptozotocin-treated rodents","volume":"18","author":"Hanani","year":"2014","journal-title":"J. Cell Mol. Med."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1683","DOI":"10.1007\/s00592-025-02500-8","article-title":"Glial fibrillary acidic protein: A potential biomarker for small fiber neuropathy?","volume":"62","author":"Hviid","year":"2025","journal-title":"Acta Diabetol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1136\/jnnp-2014-309730","article-title":"Antifibroblast growth factor receptor 3 antibodies identify a subgroup of patients with sensory neuropathy","volume":"86","author":"Antoine","year":"2015","journal-title":"J. Neurol. Neurosurg. Psychiatry."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1155\/EDR.2003.271","article-title":"Nerve Growth Factor and Diabetic Neuropathy","volume":"4","author":"Pittenger","year":"2003","journal-title":"J. Diabetes Res."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"5943","DOI":"10.12659\/MSM.909449","article-title":"Diagnostic Significance of Serum Levels of Nerve Growth Factor and Brain Derived Neurotrophic Factor in Diabetic Peripheral Neuropathy","volume":"24","author":"Sun","year":"2018","journal-title":"Med. Sci. Monit."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Shackleford, G., Marziali, L.N., Sasaki, Y., Claessens, A., Ferri, C., Weinstock, N.I., Rossor, A.M., Silvestri, N.J., Wilson, E.R., and Hurley, E. (2022). A new mouse model of Charcot-Marie-Tooth 2J neuropathy replicates human axonopathy and suggest alteration in axo-glia communication. PLoS Genet., 18.","DOI":"10.1371\/journal.pgen.1010477"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"2843","DOI":"10.1007\/s00125-021-05557-6","article-title":"Neuron-specific biomarkers predict hypo- and hyperalgesia in individuals with diabetic peripheral neuropathy","volume":"64","author":"Morgenstern","year":"2021","journal-title":"Diabetologia"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1136\/jnnp.70.1.95","article-title":"Release of biochemical markers of damage to neuronal and glial brain tissue is associated with short and long term neuropsychological outcome after traumatic brain injury","volume":"70","author":"Herrmann","year":"2001","journal-title":"J. Neurol. Neurosurg. Psychiatry"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"227","DOI":"10.2478\/cipms-2023-0039","article-title":"Potential predictive biomarker for diabetic peripheral neuropathy: Serum neuron-specific enolase","volume":"36","author":"Majeed","year":"2023","journal-title":"Curr. Issues Pharm. Med. Sci."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"3405","DOI":"10.2337\/dc13-0590","article-title":"NSE, a potential biomarker, is closely connected to diabetic peripheral neuropathy","volume":"36","author":"Li","year":"2013","journal-title":"Diabetes Care"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1007\/s13410-023-01212-5","article-title":"The relationship between neuron-specific enolase, high sensitivity C reactive protein, and diabetic peripheral neuropathy in Chinese patients with type 2 diabetes: A prospective nested case\u2013control analysis","volume":"44","author":"Xie","year":"2024","journal-title":"Int. J. Diabetes Dev. Ctries."},{"key":"ref_67","first-page":"e87214","article-title":"Association Between Serum Neuron-specific Enolase Levels and Diabetic Neuropathy in Patients with Diabetes Mellitus: A Cross-sectional Study","volume":"17","author":"Pusapati","year":"2025","journal-title":"Cureus"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Dexheimer, P.J., and Cochella, L. (2020). MicroRNAs: From Mechanism to Organism. Front. Cell Dev. Biol., 8.","DOI":"10.3389\/fcell.2020.00409"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.ncrna.2025.08.001","article-title":"Stability of microRNAs in serum and plasma reveal promise as a circulating biomarker","volume":"15","author":"Katayama","year":"2025","journal-title":"Noncoding RNA Res."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1334","DOI":"10.1097\/j.pain.0000000000002159","article-title":"A genome-wide screen reveals microRNAs in peripheral sensory neurons driving painful diabetic neuropathy","volume":"162","author":"Bali","year":"2021","journal-title":"Pain"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"2024","DOI":"10.1007\/s12015-023-10560-y","article-title":"Inflammation Modifies miR-21 Expression Within Neuronal Extracellular Vesicles to Regulate Remyelination Following Spinal Cord Injury","volume":"19","author":"Han","year":"2023","journal-title":"Stem Cell Rev. Rep."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.jns.2017.07.041","article-title":"Aberrant microRNA expression in patients with painful peripheral neuropathies","volume":"380","author":"Leinders","year":"2017","journal-title":"J. Neurol. Sci."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1530\/JME-19-0067","article-title":"MiRNA-155 silencing reduces sciatic nerve injury in diabetic peripheral neuropathy","volume":"63","author":"Chen","year":"2019","journal-title":"J. Mol. Endocrinol."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Wei, Z., Guo, C., Zhou, H., Wu, Y., Zhou, X., Chen, J., and Li, F. (2025). Exosome-mediated miRNA delivery: A molecular switch for reshaping neuropathic pain therapy. Front. Mol. Neurosci., 18.","DOI":"10.3389\/fnmol.2025.1625943"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"171","DOI":"10.2147\/DDDT.S157109","article-title":"Involvement of microRNA-146a in diabetic peripheral neuropathy through the regulation of inflammation","volume":"12","author":"Feng","year":"2018","journal-title":"Drug Des. Dev. Ther."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Fan, B., Chopp, M., Zhang, Z.G., and Liu, X.S. (2020). Emerging Roles of microRNAs as Biomarkers and Therapeutic Targets for Diabetic Neuropathy. Front. Neurol., 11.","DOI":"10.3389\/fneur.2020.558758"},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Balde\u00f3n, R.L., Weigelt, K., de Wit, H., Ozcan, B., van Oudenaren, A., Semp\u00e9rtegui, F., Sijbrands, E., Grosse, L., Freire, W., and Drexhage, H.A. (2014). Decreased serum level of miR-146a as sign of chronic inflammation in type 2 diabetic patients. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0115209"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/j.bbrc.2017.06.196","article-title":"Decreased expression of miR-150, miR146a and miR424 in type 1 diabetic patients: Association with ongoing islet autoimmunity","volume":"498","author":"Wang","year":"2018","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.1007\/s11064-020-03041-y","article-title":"Olfactory Dysfunction in Diabetic Rats is Associated with miR-146a Overexpression and Inflammation","volume":"45","author":"Estudillo","year":"2020","journal-title":"Neurochem. Res."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Khan, A., Pasquier, J., Ramachandran, V., Ponirakis, G., Petropoulos, I.N., Chidiac, O., Thomas, B., Robay, A., Jayyousi, A., and Al Suwaidi, J. (2022). Altered Circulating microRNAs in Patients with Diabetic Neuropathy and Corneal Nerve Loss: A Pilot Study. J. Clin. Med., 11.","DOI":"10.3390\/jcm11061632"},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Sehgal, R., Haacke, N., Maguolo, A., Solari, F.A., J\u00e4hnert, M., Gottmann, P., Nilsson, E., Vaag, A., Fischer-Posovszky, P., and Werberger, A. (2025). Adipose tissue-derived microRNAs as epigenetic modulators of type 2 diabetes. BMC Med., 23.","DOI":"10.1186\/s12916-025-04560-7"},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"D\u00e9ctor, M.A., Mac\u00edas-Gonz\u00e1lez, V.C., S\u00e1nchez-Garc\u00eda, A., Hern\u00e1ndez-Mendoza, A., Mart\u00ednez-Acu\u00f1a, N., Rivas-Estilla, A.M., Gonz\u00e1lez-Gonz\u00e1lez, J.G., and Barboza-Cerda, M.C. (2026). An Exploratory Biomarker Study of First-Trimester Circulating miRNAs Associated with Later Gestational Diabetes Mellitus. Int. J. Mol. Sci., 27.","DOI":"10.3390\/ijms27041920"},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Dahlin, L.B. (2023). The Dynamics of Nerve Degeneration and Regeneration in a Healthy Milieu and in Diabetes. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms242015241"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1007\/s00401-010-0697-7","article-title":"Mitochondrial biogenesis and fission in axons in cell culture and animal models of diabetic neuropathy","volume":"120","author":"Vincent","year":"2010","journal-title":"Acta Neuropathol."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.neures.2020.06.004","article-title":"Pim1 kinase provides protection against high glucose-induced stress and apoptosis in cultured dorsal root ganglion neurons","volume":"169","author":"Liu","year":"2021","journal-title":"Neurosci. Res."},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Sango, K., Mizukami, H., Horie, H., and Yagihashi, S. (2017). Impaired Axonal Regeneration in Diabetes. Perspective on the Underlying Mechanism from In Vivo and In Vitro Experimental Studies. Front. Endocrinol., 8.","DOI":"10.3389\/fendo.2017.00012"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"117459","DOI":"10.1016\/j.lfs.2020.117459","article-title":"Schwann cells apoptosis is induced by high glucose in diabetic peripheral neuropathy","volume":"248","author":"Pu","year":"2020","journal-title":"Life Sci."},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Taveggia, C., and Bolino, A. (2018). DRG Neuron\/Schwann Cells Myelinating Cocultures. Methods in Molecular Biology, Humana Press.","DOI":"10.1007\/978-1-4939-7862-5_9"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1038\/nrneurol.2016.201","article-title":"Schwann cell interactions with axons and microvessels in diabetic neuropathy","volume":"13","author":"Andersen","year":"2017","journal-title":"Nat. Rev. Neurol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"2397","DOI":"10.2337\/db10-1462","article-title":"Beneficial Effects of Exendin-4 on Experimental Polyneuropathy in Diabetic Mice","volume":"60","author":"Himeno","year":"2011","journal-title":"Diabetes"},{"key":"ref_91","doi-asserted-by":"crossref","unstructured":"Taiana, M.M., Lombardi, R., Porretta-Serapiglia, C., Ciusani, E., Oggioni, N., Sassone, J., Bianchi, R., and Lauria, G. (2014). Neutralization of Schwann Cell-Secreted VEGF Is Protective to In Vitro and In Vivo Experimental Diabetic Neuropathy. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0108403"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1111\/ejn.13054","article-title":"In vitro models for peripheral nerve regeneration","volume":"43","author":"Geuna","year":"2016","journal-title":"Eur. J. Neurosci."},{"key":"ref_93","first-page":"5289","article-title":"Nanotechnology versus stem cell engineering: In vitro comparison of neurite inductive potentials","volume":"9","author":"Morano","year":"2014","journal-title":"Int. J. Nanomed."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1111\/bju.14834","article-title":"New basic insights on the potential of a chitosan-based medical device for improving functional recovery after radical prostatectomy","volume":"124","author":"Muratori","year":"2019","journal-title":"BJU Int."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1038\/s41392-024-01809-0","article-title":"Induced pluripotent stem cells (iPSCs): Molecular mechanisms of induction and applications","volume":"9","author":"Cerneckis","year":"2024","journal-title":"Signal Transduct. Target Ther."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1038\/nbt.2249","article-title":"Combined small-molecule inhibition accelerates developmental timing and converts human pluripotent stem cells into nociceptors","volume":"30","author":"Chambers","year":"2012","journal-title":"Nat. Biotechnol."},{"key":"ref_97","doi-asserted-by":"crossref","unstructured":"Schoepf, C.L., Zeidler, M., Spiecker, L., Kern, G., Lechner, J., Kummer, K.K., and Kress, M. (2020). Selected Ionotropic Receptors and Voltage-Gated Ion Channels: More Functional Competence for Human Induced Pluripotent Stem Cell (iPSC)-Derived Nociceptors. Brain Sci., 10.","DOI":"10.3390\/brainsci10060344"},{"key":"ref_98","doi-asserted-by":"crossref","unstructured":"H\u00f6rner, S.J., Couturier, N., Bruch, R., Koch, P., Hafner, M., and Rudolf, R. (2021). HiPSC-Derived Schwann Cells Influence Myogenic Differentiation in Neuromuscular Cocultures. Cells, 10.","DOI":"10.3390\/cells10123292"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"1714","DOI":"10.1016\/j.stemcr.2017.04.011","article-title":"Schwann Cell Precursors from Human Pluripotent Stem Cells as a Potential Therapeutic Target for Myelin Repair","volume":"8","author":"Kim","year":"2017","journal-title":"Stem Cell Rep."},{"key":"ref_100","doi-asserted-by":"crossref","unstructured":"Huang, Z., Powell, R., Kankowski, S., Phillips, J.B., and Haastert-Talini, K. (2023). Culture Conditions for Human Induced Pluripotent Stem Cell-Derived Schwann Cells: A Two-Centre Study. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24065366"},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1186\/s13287-026-04965-y","article-title":"Targeting p75NTR activity alleviates the neurotoxic effect of high glucose on iPSC-derived dopaminergic neurons","volume":"17","author":"Chanoumidou","year":"2026","journal-title":"Stem Cell Res. Ther."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"31567","DOI":"10.1021\/acsami.2c03861","article-title":"Development of an In Vitro Biomimetic Peripheral Neurovascular Platform","volume":"14","author":"Malheiro","year":"2022","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_103","doi-asserted-by":"crossref","unstructured":"Pandey, S., Jir\u00e1sko, M., Lochman, J., Chv\u00e1tal, A., Chottova Dvorakova, M., and Ku\u010dera, R. (2022). IPSCs in Neurodegenerative Disorders: A Unique Platform for Clinical Research and Personalized Medicine. J. Pers. Med., 12.","DOI":"10.3390\/jpm12091485"},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"1348","DOI":"10.1038\/s12276-024-01250-x","article-title":"Advances and challenges in modeling inherited peripheral neuropathies using iPSCs","volume":"56","author":"Prior","year":"2024","journal-title":"Exp. Mol. Med."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/s41232-024-00319-4","article-title":"Novel artificial nerve transplantation of human iPSC-derived neurite bundles enhanced nerve regeneration after peripheral nerve injury","volume":"44","author":"Nishijima","year":"2024","journal-title":"Inflamm. Regen."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1038\/s41574-022-00797-x","article-title":"Towards a better understanding of diabetes mellitus using organoid models","volume":"19","author":"Yennek","year":"2023","journal-title":"Nat. Rev. Endocrinol."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"160","DOI":"10.3344\/kjp.2022.35.2.160","article-title":"Insulin enhances neurite extension and myelination of diabetic neuropathy neurons","volume":"35","author":"Pham","year":"2022","journal-title":"Korean J. Pain"},{"key":"ref_108","doi-asserted-by":"crossref","unstructured":"Yang, Y., Wang, T.-T., Xie, H.-A., Hu, P.P., and Li, P. (2024). Experimental cell models of insulin resistance: Overview and appraisal. Front. Endocrinol., 15.","DOI":"10.3389\/fendo.2024.1469565"},{"key":"ref_109","doi-asserted-by":"crossref","unstructured":"Pavlacky, J., and Polak, J. (2020). Technical Feasibility and Physiological Relevance of Hypoxic Cell Culture Models. Front. Endocrinol., 11.","DOI":"10.3389\/fendo.2020.00057"},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"799","DOI":"10.52586\/4989","article-title":"The biological effect of cobalt chloride mimetic-hypoxia on nucleus pulposus cells and the comparability with physical hypoxia in vitro","volume":"26","author":"Gao","year":"2021","journal-title":"Front. Biosci.-Landmark"},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"1738","DOI":"10.1096\/fj.01-1027com","article-title":"High glucose-induced oxidative stress and mitochondrial dysfunction in neurons","volume":"16","author":"Russell","year":"2002","journal-title":"FASEB J."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1096\/fj.04-2513fje","article-title":"Short-term hyperglycemia produces oxidative damage and apoptosis in neurons","volume":"19","author":"Vincent","year":"2005","journal-title":"FASEB J."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1111\/j.1749-6632.2002.tb02108.x","article-title":"Oxidative Stress and Programmed Cell Death in Diabetic Neuropathy","volume":"959","author":"Vincent","year":"2002","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_114","first-page":"1","article-title":"Diabetic Polyneuropathy in Type 2 Diabetes Mellitus: Inflammation, Oxidative Stress, and Mitochondrial Function","volume":"2016","year":"2016","journal-title":"J. Diabetes Res."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1586\/eem.09.55","article-title":"Mitochondrial stress and the pathogenesis of diabetic neuropathy","volume":"5","author":"Fernyhough","year":"2010","journal-title":"Expert Rev. Endocrinol. Metab."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"3521","DOI":"10.1113\/JP270874","article-title":"The repair Schwann cell and its function in regenerating nerves","volume":"594","author":"Jessen","year":"2016","journal-title":"J. Physiol."},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.1093\/brain\/aws097","article-title":"Impaired adenosine monophosphate-activated protein kinase signalling in dorsal root ganglia neurons is linked to mitochondrial dysfunction and peripheral neuropathy in diabetes","volume":"135","author":"Smith","year":"2012","journal-title":"Brain"},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"17106","DOI":"10.1074\/jbc.M114.631291","article-title":"Hyperglycemia Promotes Schwann Cell De-differentiation and De-myelination via Sorbitol Accumulation and Igf1 Protein Down-regulation","volume":"290","author":"Hao","year":"2015","journal-title":"J. Biol. Chem."},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1155\/EDR.2003.235","article-title":"The Insulin-Like Growth Factor System and Neurological Complications in Diabetes","volume":"4","author":"Sima","year":"2003","journal-title":"J. Diabetes Res."},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"547834","DOI":"10.1155\/2015\/547834","article-title":"The Association between Serum Cytokines and Damage to Large and Small Nerve Fibers in Diabetic Peripheral Neuropathy","volume":"2015","author":"Magrinelli","year":"2015","journal-title":"J. Diabetes Res."},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1007\/BF00403326","article-title":"Hypoxic neuropathy: Relevance to human diabetic neuropathy","volume":"33","author":"Malik","year":"1990","journal-title":"Diabetologia"},{"key":"ref_122","doi-asserted-by":"crossref","unstructured":"Pucha\u0142owicz, K., and Ra\u0107, M.E. (2020). The Multifunctionality of CD36 in Diabetes Mellitus and Its Complications\u2014Update in Pathogenesis, Treatment and Monitoring. Cells, 9.","DOI":"10.3390\/cells9081877"},{"key":"ref_123","first-page":"148","article-title":"The synergistic effect of palmitic acid and glucose on inducing endoplasmic reticulum stress-associated apoptosis in rat Schwann cells","volume":"26","author":"Yang","year":"2022","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/S1672-0229(06)60033-8","article-title":"Optimal Allocation of Replicates for Measurement Evaluation Studies","volume":"4","author":"Zakharkin","year":"2006","journal-title":"Genom. Proteom. Bioinform."},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1210\/en.2006-0073","article-title":"Receptor for Advanced Glycation End Products Activation Injures Primary Sensory Neurons via Oxidative Stress","volume":"148","author":"Vincent","year":"2007","journal-title":"Endocrinology"},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"6431459","DOI":"10.1155\/2020\/6431459","article-title":"Neuroprotective Effect of Salvianolic Acid A against Diabetic Peripheral Neuropathy through Modulation of Nrf2","volume":"2020","author":"Xu","year":"2020","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"931","DOI":"10.2337\/db12-0632","article-title":"RAGE Deficiency Improves Postinjury Sciatic Nerve Regeneration in Type 1 Diabetic Mice","volume":"62","author":"Juranek","year":"2013","journal-title":"Diabetes"},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"1741","DOI":"10.1172\/JCI18058","article-title":"Loss of pain perception in diabetes is dependent on a receptor of the immunoglobulin superfamily","volume":"114","author":"Bierhaus","year":"2004","journal-title":"J. Clin. Investig."},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"102852","DOI":"10.1016\/j.xpro.2024.102852","article-title":"Protocol for isolating and processing mouse sciatic nerve fibers for confocal immunohistochemistry","volume":"5","author":"Sell","year":"2024","journal-title":"STAR Protoc."},{"key":"ref_130","first-page":"A186","article-title":"Using ImageJ to Assess Neurite Outgrowth in Mammalian Cell Cultures: Research Data Quantification Exercises in Undergraduate Neuroscience Lab","volume":"16","author":"Pemberton","year":"2018","journal-title":"J. Undergrad. Neurosci. Educ."},{"key":"ref_131","doi-asserted-by":"crossref","unstructured":"Ho, S.Y., Chao, C.Y., Huang, H.L., Chiu, T.W., Charoenkwan, P., and Hwang, E. (2011). NeurphologyJ: An automatic neuronal morphology quantification method and its application in pharmacological discovery. BMC Bioinform., 12.","DOI":"10.1186\/1471-2105-12-230"},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1007\/s00109-023-02347-y","article-title":"Novel insights into the nervous system affected by prolonged hyperglycemia","volume":"101","author":"Mukherjee","year":"2023","journal-title":"J. Mol. Med."},{"key":"ref_133","doi-asserted-by":"crossref","unstructured":"Miyashita, A., Kobayashi, M., Yokota, T., and Zochodne, D. (2023). Diabetic Polyneuropathy: New Strategies to Target Sensory Neurons in Dorsal Root Ganglia. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24065977"},{"key":"ref_134","doi-asserted-by":"crossref","unstructured":"Wu, L., Wang, X.J., Luo, X., Zhang, J., Zhao, X., and Chen, Q. (2024). Diabetic peripheral neuropathy based on Schwann cell injury: Mechanisms of cell death regulation and therapeutic perspectives. Front. Endocrinol., 15.","DOI":"10.3389\/fendo.2024.1427679"},{"key":"ref_135","doi-asserted-by":"crossref","unstructured":"Nashtahosseini, Z., Eslami, M., Paraandavaji, E., Haraj, A., Dowlat, B.F., Hosseinzadeh, E., Oksenych, V., and Naderian, R. (2025). Cytokine Signaling in Diabetic Neuropathy: A Key Player in Peripheral Nerve Damage. Biomedicines, 13.","DOI":"10.3390\/biomedicines13030589"},{"key":"ref_136","doi-asserted-by":"crossref","first-page":"29341","DOI":"10.1074\/jbc.M404167200","article-title":"Early Painful Diabetic Neuropathy Is Associated with Differential Changes in Tetrodotoxin-sensitive and -resistant Sodium Channels in Dorsal Root Ganglion Neurons in the Rat","volume":"279","author":"Hong","year":"2004","journal-title":"J. Biol. Chem."},{"key":"ref_137","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1007\/s11095-021-03075-z","article-title":"Multi-Electrode Array of Sensory Neurons as an In Vitro Platform to Identify the Nociceptive Response to Pharmaceutical Buffer Systems of Injectable Biologics","volume":"38","author":"Eaton","year":"2021","journal-title":"Pharm. Res."},{"key":"ref_138","first-page":"123","article-title":"Innovative microfluidic technologies in precision research and therapy development in diabetic neuropathy: A narrative review","volume":"1","author":"Lee","year":"2024","journal-title":"Adv. Technol. Neurosci."},{"key":"ref_139","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1038\/s41582-024-01041-y","article-title":"The global and regional burden of diabetic peripheral neuropathy","volume":"21","author":"Savelieff","year":"2025","journal-title":"Nat. Rev. Neurol."},{"key":"ref_140","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1038\/s41582-018-0058-z","article-title":"Neurofilaments as biomarkers in neurological disorders","volume":"14","author":"Khalil","year":"2018","journal-title":"Nat. Rev. Neurol."},{"key":"ref_141","doi-asserted-by":"crossref","unstructured":"Yang, H., Sloan, G., Ye, Y., Wang, S., Duan, B., Tesfaye, S., and Gao, L. (2019). New Perspective in Diabetic Neuropathy: From the Periphery to the Brain, a Call for Early Detection, and Precision Medicine. Front. Endocrinol., 10.","DOI":"10.3389\/fendo.2019.00929"},{"key":"ref_142","doi-asserted-by":"crossref","first-page":"101972","DOI":"10.1016\/j.mito.2024.101972","article-title":"Mitochondrial dysfunction in diabetic neuropathy: Impaired mitophagy triggers NLRP3 inflammasome","volume":"79","author":"Sriwastawa","year":"2024","journal-title":"Mitochondrion"},{"key":"ref_143","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1210\/er.2018-00107","article-title":"Emerging Biomarkers, Tools, and Treatments for Diabetic Polyneuropathy","volume":"40","author":"Herder","year":"2019","journal-title":"Endocr. Rev."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/18\/8234\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T08:05:40Z","timestamp":1789545940000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/18\/8234"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,16]]},"references-count":143,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["ijms27188234"],"URL":"https:\/\/doi.org\/10.3390\/ijms27188234","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,16]]}}}