{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T09:02:51Z","timestamp":1789549371607,"version":"build-2803163510"},"reference-count":31,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Evaluation, Innovation, and Excellent Gene Excavation for Elite Soybean Cultivars between China and Europe","award":["2019YFE0105900"],"award-info":[{"award-number":["2019YFE0105900"]}]},{"name":"Research Expenses of Provincial Research Institutes of Heilongjiang Province","award":["CZKYF2023-1-C002"],"award-info":[{"award-number":["CZKYF2023-1-C002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Porcine reproductive and respiratory syndrome virus (PRRSV) has become a global health threat for swine. Because of the limited effectiveness of commercial vaccine strategies and the absence of specific antiviral chemical drugs for PRRS, it is urgent to identify new strategies for preventing and controlling PRRSV infections. Genistein, a soy-derived isoflavone, has shown promise in reducing viremia in PRRSV-infected pigs. However, its antiviral molecular mechanism remains unclear. This study investigates the inhibitory effects and mechanism of genistein on PRRSV infection in vitro. Our results demonstrated that genistein dose-dependently inhibited PRRSV infection in Marc-145 cells at several stages of the PRRSV replication cycle. With its favorable safety profile and potent efficacy in antiviral and growth promotion in swine, genistein stands out as a promising dietary adjunct for PRRSV prevention and control in swine husbandry.<\/jats:p>","DOI":"10.3390\/ijms27188235","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T07:58:59Z","timestamp":1789545539000},"page":"8235","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Soy-Derived Isoflavone Genistein Inhibits the Life Cycle of PRRSV In Vitro"],"prefix":"10.3390","volume":"27","author":[{"given":"Dan","family":"Cao","sequence":"first","affiliation":[{"name":"Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9904-8893","authenticated-orcid":false,"given":"Changchao","family":"Huan","sequence":"additional","affiliation":[{"name":"Institute of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China"},{"name":"Key Laboratory of Avian Bioproduct Development, Ministry of Agriculture and Rural Affairs, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofei","family":"Tang","sequence":"additional","affiliation":[{"name":"Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongguo","family":"Xue","sequence":"additional","affiliation":[{"name":"Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.virusres.2010.10.009","article-title":"Porcine reproductive and respiratory syndrome virus: An update on an emerging and re-emerging viral disease of swine","volume":"154","author":"Lunney","year":"2010","journal-title":"Virus Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.virusres.2010.07.016","article-title":"Porcine reproductive and respiratory syndrome in China","volume":"154","author":"Zhou","year":"2010","journal-title":"Virus Res."},{"key":"ref_3","first-page":"11","article-title":"PRRSV, the virus","volume":"31","author":"Meulenberg","year":"2000","journal-title":"Vet. 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