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J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Glaucoma drainage device (GDD) surgery is frequently limited by postoperative fibroblast proliferation, leading to device failure and the need for revision procedures. In this work, we developed a spray-assisted layer-by-layer (LbL) drug delivery system for GDD surfaces intended to enable localized release of antiproliferative agents, as a proof-of-concept approach toward more standardized patient care. Multilayer thin films composed of poly(\u03b2-amino ester) (PBAE) and a 5-fluorouracil (5-FU) and \u03b2-cyclodextrin (\u03b2-CD) complex (5-FU:\u03b2-CD) were fabricated, without (type A films) and with (type B films) graphene oxide (GO) barrier layers. Film growth was monitored by ultraviolet\u2013visible spectroscopy (UV-Vis) and vacuum ultraviolet spectroscopy (VUV), confirming consistent, sequential deposition; type A films exhibited lower variability than GO-containing type B films. Drug release studies revealed a rapid burst release within the first 5 min, followed by a plateau, regardless of GO incorporation, indicating that sustained drug release was not achieved under the current film architecture. Cell cycle analysis confirmed that \u03b2-CD complexation preserved the biological activity of 5-FU, which induced an S-phase arrest in a dose-dependent manner. Although further optimization of film architecture and composition is required to improve release control and achieve sustained delivery, this study demonstrates the feasibility of spray-assisted LbL coatings as a localized drug delivery strategy for GDD surfaces. Overall, these findings support the proof-of-concept nature of this approach and identify key parameters that must be optimized before translational applicability can be established.<\/jats:p>","DOI":"10.3390\/ijms27188257","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T16:07:29Z","timestamp":1789574849000},"page":"8257","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["From Fabrication to Function: Scalable Spray-Assisted Layer-by-Layer Films for Drug Delivery in Glaucoma"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5842-8550","authenticated-orcid":false,"given":"Alexandra M. L.","family":"Oliveira","sequence":"first","affiliation":[{"name":"NOVA Medical School, Faculdade de Ci\u00eancias M\u00e9dicas (NMS, FCM), Universidade Nova de Lisboa, 1160-056 Lisboa, Portugal"},{"name":"iNOVA4Health, NOVA Medical School, Faculdade de Ci\u00eancias M\u00e9dicas (NMS, FCM), Universidade Nova de Lisboa, 1160-056 Lisboa, 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Future","volume":"12","author":"Allison","year":"2020","journal-title":"Cureus"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1901","DOI":"10.1001\/jama.2014.3192","article-title":"The Pathophysiology and Treatment of Glaucoma: A Review","volume":"311","author":"Weinreb","year":"2014","journal-title":"JAMA"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.preteyeres.2004.08.003","article-title":"Understanding mechanisms of pressure-induced optic nerve damage","volume":"24","author":"Morrison","year":"2005","journal-title":"Prog. Retin. Eye Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"6795","DOI":"10.1038\/s41598-022-10269-x","article-title":"Structural and functional differentiation between compressive and glaucomatous optic neuropathy","volume":"12","author":"Laowanapiban","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2183","DOI":"10.1016\/S0140-6736(17)31469-1","article-title":"Glaucoma","volume":"390","author":"Jonas","year":"2017","journal-title":"Lancet"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1788","DOI":"10.1016\/S0140-6736(23)01289-8","article-title":"Glaucoma: Now and beyond","volume":"402","author":"Jayaram","year":"2023","journal-title":"Lancet"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1803","DOI":"10.1016\/S0140-6736(99)04240-3","article-title":"Glaucoma","volume":"354","author":"Coleman","year":"1999","journal-title":"Lancet"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1097\/IJG.0000000000002341","article-title":"Comparison of Structural and Functional Features in Primary Angle Closure and Open Angle Glaucomas","volume":"33","author":"Sun","year":"2024","journal-title":"J. Glaucoma"},{"key":"ref_9","first-page":"225","article-title":"The Diagnosis and Treatment of Glaucoma","volume":"117","author":"Schuster","year":"2020","journal-title":"Dtsch. Arztebl. Int."},{"key":"ref_10","first-page":"728","article-title":"Congenital glaucoma","volume":"58","author":"Cibis","year":"1987","journal-title":"J. Am. Optom. Assoc."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"525","DOI":"10.4103\/ijo.IJO_1591_20","article-title":"Neovascular glaucoma\u2014A review","volume":"69","author":"Senthil","year":"2021","journal-title":"Indian J. Ophthalmol."},{"key":"ref_12","first-page":"375","article-title":"Uveitic glaucoma","volume":"15","author":"Kok","year":"2002","journal-title":"Ophthalmol. Clin."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1016\/j.survophthal.2020.01.002","article-title":"Steroid-induced glaucoma: Epidemiology, pathophysiology, and clinical management","volume":"65","author":"Roberti","year":"2020","journal-title":"Surv. Ophthalmol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1007\/BF00161770","article-title":"Lens-induced glaucoma","volume":"81","author":"Ellant","year":"1992","journal-title":"Doc. Ophthalmol."},{"key":"ref_15","first-page":"215","article-title":"Ocular trauma-related glaucoma","volume":"15","author":"Girkin","year":"2002","journal-title":"Ophthalmol. Clin."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1097\/00055735-200204000-00001","article-title":"Genetic basis of glaucoma","volume":"13","author":"WuDunn","year":"2002","journal-title":"Curr. Opin. Ophthalmol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1111\/cge.14603","article-title":"Childhood glaucoma: Implications for genetic counselling","volume":"106","author":"Maxwell","year":"2024","journal-title":"Clin. Genet."},{"key":"ref_18","first-page":"CD010520","article-title":"Topical medication instillation techniques for glaucoma","volume":"2","author":"Xu","year":"2017","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1111\/aos.16599","article-title":"Guidelines for the management of open-angle glaucoma: National Program Area Eye Diseases, National Working Group Glaucoma","volume":"102","author":"Stille","year":"2024","journal-title":"Acta Ophthalmol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Patton, G.N., and Lee, H.J. (2024). Chemical Insights into Topical Agents in Intraocular Pressure Management: From Glaucoma Etiopathology to Therapeutic Approaches. Pharmaceutics, 16.","DOI":"10.3390\/pharmaceutics16020274"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.ejphar.2004.06.067","article-title":"Effects of neurosteroids on ischemia-reperfusion injury in the rat retina: Role of sigma1 recognition sites","volume":"498","author":"Bucolo","year":"2004","journal-title":"Eur. J. Pharmacol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1002\/dneu.20373","article-title":"Retinal ganglion cell protection by 17-beta-estradiol in a mouse model of inherited glaucoma","volume":"67","author":"Zhou","year":"2007","journal-title":"Dev. Neurobiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3253","DOI":"10.1021\/mp400313u","article-title":"17\u03b2-estradiol eye drops protect the retinal ganglion cell layer and preserve visual function in an in vivo model of glaucoma","volume":"10","author":"Xin","year":"2013","journal-title":"Mol. Pharm."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Kapic, A., Zaman, K., Nguyen, V., Neagu, G.C., Sumien, N., Prokai, L., and Prokai-Tatrai, K. (2024). The Prodrug DHED Delivers 17\u03b2-Estradiol into the Retina for Protection of Retinal Ganglion Cells and Preservation of Visual Function in an Animal Model of Glaucoma. Cells, 13.","DOI":"10.3390\/cells13131126"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1097\/01.icu.0000156139.05323.26","article-title":"Compliance and persistency in glaucoma follow-up treatment","volume":"16","author":"Schwartz","year":"2005","journal-title":"Curr. Opin. Ophthalmol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1418","DOI":"10.4103\/ijo.IJO_2008_20","article-title":"An exploratory study of compliance to anti-glaucoma medications among literate primary glaucoma patients at an urban tertiary eye care center in South India","volume":"69","author":"Ramesh","year":"2021","journal-title":"Indian J. Ophthalmol."},{"key":"ref_27","first-page":"e63475","article-title":"Level of Adherence to Glaucoma Medication and Its Associated Factors Among Adult Jordanian Patients","volume":"16","author":"Alhusban","year":"2024","journal-title":"Cureus"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1097\/01.icu.0000193078.47616.aa","article-title":"Ethnic differences in primary angle-closure glaucoma","volume":"17","author":"Yip","year":"2006","journal-title":"Curr. Opin. Ophthalmol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.ophtha.2022.09.009","article-title":"Laser in Glaucoma and Ocular Hypertension (LiGHT) Trial: Six-Year Results of Primary Selective Laser Trabeculoplasty versus Eye Drops for the Treatment of Glaucoma and Ocular Hypertension","volume":"130","author":"Gazzard","year":"2023","journal-title":"Ophthalmology"},{"key":"ref_30","first-page":"493","article-title":"Cyclodestruction and cyclophotocoagulation: Where are we?","volume":"99","year":"2024","journal-title":"Arch. Soc. Esp. Oftalmol. (Engl. Ed.)"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1097\/IIO.0000000000000231","article-title":"Trabeculectomy: A Brief History and Review of Current Trends","volume":"58","author":"Koike","year":"2018","journal-title":"Int. Ophthalmol. Clin."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"357","DOI":"10.2147\/OPTH.S104220","article-title":"Ahmed Glaucoma Valve Implant: Surgical Technique and Complications","volume":"11","author":"Riva","year":"2017","journal-title":"Clin. Ophthalmol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"16646","DOI":"10.1038\/s41598-025-00839-0","article-title":"Efficacy and safety outcomes of the Paul glaucoma implant compared to the Ahmed glaucoma valve","volume":"15","author":"Stingl","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Balas, M., and Mathew, D.J. (2023). Minimally Invasive Glaucoma Surgery: A Review of the Literature. Vision, 7.","DOI":"10.3390\/vision7030054"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2447","DOI":"10.1007\/s00417-025-06861-2","article-title":"The Paul Glaucoma Implant: A systematic review of safety, efficacy, and emerging applications","volume":"263","author":"Gambini","year":"2025","journal-title":"Graefe\u2019s Arch. Clin. Exp. Ophthalmol."},{"key":"ref_36","first-page":"1937","article-title":"iStent trabecular micro-bypass stent for open-angle glaucoma","volume":"8","author":"Le","year":"2014","journal-title":"Clin. Ophthalmol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Sacchi, M., Tomaselli, D., Ruggeri, M.L., Aiello, F.B., Sabella, P., Dore, S., Pinna, A., Mastropasqua, R., Nubile, M., and Agnifili, L. (2025). Fighting Bleb Fibrosis After Glaucoma Surgery: Updated Focus on Key Players and Novel Targets for Therapy. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26052327"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1579","DOI":"10.18240\/ijo.2025.08.21","article-title":"Mechanisms of fibrosis formation following glaucoma filtration surgery","volume":"18","author":"Zhang","year":"2025","journal-title":"Int. J. Ophthalmol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Mill\u00e1, E., Ventura-Abreu, N., Vendrell, C., Muniesa, M.J., Pazos, M., Gasull, X., and Comes, N. (2023). Differential Gene and Protein Expression of Conjunctival Bleb Hyperfibrosis in Early Failure of Glaucoma Surgery. Int. J. Mol. Sci., 24.","DOI":"10.20944\/preprints202306.0545.v1"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1007\/s00347-016-0372-z","article-title":"Revision nach Filtrationschirurgie","volume":"113","author":"Rejdak","year":"2016","journal-title":"Ophthalmologe"},{"key":"ref_41","first-page":"79","article-title":"Mitomycin C in glaucoma surgery","volume":"247","year":"1993","journal-title":"Bull. Soc. Belge. Ophtalmol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"320","DOI":"10.4103\/IJO.IJO_2013_23","article-title":"Wound modulation in glaucoma surgery: The role of anti-scarring agents","volume":"72","author":"Kavitha","year":"2024","journal-title":"Indian. J. Ophthalmol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1016\/S0161-6420(87)33321-4","article-title":"Glaucoma filtering surgery with 5-fluorouracil","volume":"94","author":"Rockwood","year":"1987","journal-title":"Ophthalmology"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"55","DOI":"10.4103\/meajo.meajo_33_23","article-title":"Corneal Endothelial Toxicity Secondary to Topical 5-fluorouracil","volume":"30","author":"Alsaleh","year":"2023","journal-title":"Middle East. Afr. J. Ophthalmol."},{"key":"ref_45","first-page":"580","article-title":"5-Fluorouracil toxicity to the ocular surface epithelium","volume":"26","author":"Shapiro","year":"1985","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1016\/S0161-6420(95)30875-5","article-title":"Mitomycin C versus 5-fluorouracil in high-risk glaucoma filtering surgery. Extended follow-up","volume":"102","author":"Katz","year":"1995","journal-title":"Ophthalmology"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1076\/ceyr.22.3.174.5513","article-title":"Mechanism of growth inhibitory effect of Mitomycin-C on cultured human retinal pigment epithelial cells: Apoptosis and cell cycle arrest","volume":"22","author":"Kang","year":"2001","journal-title":"Curr. Eye Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"503252","DOI":"10.1016\/j.mrgentox.2020.503252","article-title":"Mitomycin C induced genotoxic stress in endothelial cells is associated with differential expression of proinflammatory cytokines","volume":"858\u2013860","author":"Sinitsky","year":"2020","journal-title":"Mutat. Res. Genet. Toxicol. Environ. Mutagen."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.3390\/molecules13081551","article-title":"5-Fluorouracil: Mechanisms of Resistance and Reversal Strategies","volume":"13","author":"Zhang","year":"2008","journal-title":"Molecules"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"211","DOI":"10.4103\/joco.joco_283_23","article-title":"Use of Mitomycin C in Ophthalmic Surgery","volume":"36","author":"Nassiri","year":"2024","journal-title":"J. Curr. Ophthalmol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1097\/ICU.0000000000000729","article-title":"History, presence, and future of mitomycin C in glaucoma filtration surgery","volume":"32","author":"Wolters","year":"2021","journal-title":"Curr. Opin. Ophthalmol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Assun\u00e7\u00e3o, I.C.C., S\u00e9rio, S., Ferreira, Q., Jones, N.C., Hoffmann, S.V., Ribeiro, P.A., and Raposo, M. (2021). Graphene Oxide Layer-by-Layer Films for Sensors and Devices. Nanomaterials, 11.","DOI":"10.3390\/nano11061556"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Oliveira, A.M.L., Machado, M., Silva, G.A., Bitoque, D.B., Tavares Ferreira, J., Pinto, L.A., and Ferreira, Q. (2022). Graphene Oxide Thin Films with Drug Delivery Function. Nanomaterials, 12.","DOI":"10.3390\/nano12071149"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"4173","DOI":"10.1021\/acsabm.9b00417","article-title":"Self-Assembled Multilayer Films for Time-Controlled Ocular Drug Delivery","volume":"2","author":"Machado","year":"2019","journal-title":"ACS Appl. Bio Mater."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"10761","DOI":"10.1021\/ja0015388","article-title":"Degradable Poly(\u03b2-amino esters): Synthesis, Characterization, and Self-Assembly with Plasmid DNA","volume":"122","author":"Lynn","year":"2000","journal-title":"J. Am. Chem. Soc."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Melnikova, D.L., Badrieva, Z.F., Kostin, M.A., Maller, C., Stas, M., Buczek, A., Broda, M.A., Kupka, T., Kelterer, A.M., and Tolstoy, P.M. (2020). On Complex Formation between 5-Fluorouracil and \u03b2-Cyclodextrin in Solution and in the Solid State: IR Markers and Detection of Short-Lived Complexes by Diffusion NMR. Molecules, 25.","DOI":"10.3390\/molecules25235706"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Di Donato, C., Lavorgna, M., Fattorusso, R., Isernia, C., Isidori, M., Malgieri, G., Piscitelli, C., Russo, C., Russo, L., and Iacovino, R. (2016). Alpha- and Beta-Cyclodextrin Inclusion Complexes with 5-Fluorouracil: Characterization and Cytotoxic Activity Evaluation. Molecules, 21.","DOI":"10.3390\/molecules21121644"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"33522","DOI":"10.1039\/D5RA03005F","article-title":"Enhancement of the solubility of 5-fluorouracil through encapsulation within \u03b2-cyclodextrin to control fibroblast growth in glaucoma surgery","volume":"15","author":"Machado","year":"2025","journal-title":"RSC Adv."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"11176","DOI":"10.1021\/ie100990z","article-title":"Preparation of 5-Fluorouracil\/\u03b2-Cyclodextrin Complex Intercalated in Layered Double Hydroxide and the Controlled Drug Release Properties","volume":"49","author":"Jin","year":"2010","journal-title":"Ind. Eng. Chem. Res."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"20221019","DOI":"10.1259\/bjr.20221019","article-title":"A systematic review of repeatability and reproducibility studies of diffusion tensor imaging of cervical spinal cord","volume":"96","author":"Fulford","year":"2023","journal-title":"Br. J. Radiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"10099","DOI":"10.1021\/acsomega.3c05966","article-title":"Layer-by-Layer Biopolymer Assembly for the In Situ Fabrication of AuNP Plasmonic Paper-A SERS Substrate for Food Adulteration Detection","volume":"9","author":"Viriyakitpattana","year":"2024","journal-title":"ACS Omega"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1007\/s13239-018-0346-1","article-title":"Coating and Pharmacokinetic Evaluation of Air Spray Coated Drug Coated Balloons","volume":"9","author":"Turner","year":"2018","journal-title":"Cardiovasc. Eng. Technol."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Kittle, J., Levin, J., and Levin, N. (2021). Water Content of Polyelectrolyte Multilayer Films Measured by Quartz Crystal Microbalance and Deuterium Oxide Exchange. Sensors, 21.","DOI":"10.3390\/s21030771"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Serebrennikova, K.V., Komova, N.S., Zherdev, A.V., and Dzantiev, B.B. (2024). SERS Sensors with Bio-Derived Substrates Under the Way to Agricultural Monitoring of Pesticide Residues. Biosensors, 14.","DOI":"10.3390\/bios14120573"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"6645","DOI":"10.1007\/s00289-021-03804-9","article-title":"In vitro release and cytotoxicity activity of 5-fluorouracil entrapped polycaprolactone nanoparticles","volume":"79","author":"Samy","year":"2022","journal-title":"Polym. Bull."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/1476-4598-5-20","article-title":"Cellular response to 5-fluorouracil (5-FU) in 5-FU-resistant colon cancer cell lines during treatment and recovery","volume":"5","author":"Svendsrud","year":"2006","journal-title":"Mol. Cancer"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"231","DOI":"10.3727\/096504001108747729","article-title":"Differences in the induction of DNA damage, cell cycle arrest, and cell death by 5-fluorouracil and antifolates","volume":"12","author":"Backus","year":"2000","journal-title":"Oncol. Res."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"2432","DOI":"10.1021\/nn2047197","article-title":"Highly Tunable Charge Transport in Layer-by-Layer Assembled Graphene Transistors","volume":"6","author":"Hwang","year":"2012","journal-title":"ACS Nano"},{"key":"ref_69","unstructured":"National Center for Biotechnology Information (2005). PubChem Compound Summary for CID 3385, 5-Fluorouracil, National Library of Medicine."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Pirvu, A.S., Varut, R.-M., Trasca, D.-M., Stoica, G.A., Radivojevic, K., Carmen, S., Arsenie, C.C., and Popescu, C. (2025). Cyclodextrins as Active Therapeutic Agents: Beyond Their Role as Excipients. Pharmaceuticals, 18.","DOI":"10.3390\/ph18101592"},{"key":"ref_71","unstructured":"Morais, H.I.C. (2017). Nanostructured Films of Graphene for Controlled Ocular Drug Delivery, Instituto Polit\u00e9cnico de Lisboa."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Tadyszak, K., Wychowaniec, J.K., and Litowczenko, J. (2018). Biomedical Applications of Graphene-Based Structures. Nanomaterials, 8.","DOI":"10.3390\/nano8110944"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1002\/smll.201101941","article-title":"Functionalization of Amorphous SiO2 and 6H-SiC(0001) Surfaces with Benzo[ghi]perylene-1,2-dicarboxylic Anhydride via an APTES Linker","volume":"8","author":"Bhowmick","year":"2012","journal-title":"Small"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"8425","DOI":"10.1021\/la501877v","article-title":"Adsorption and Surfactant-Mediated Desorption of Poly(vinylpyrrolidone) on Plasma- and Piranha-Cleaned Silica Surfaces","volume":"30","author":"Cattoz","year":"2014","journal-title":"Langmuir"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"100818","DOI":"10.1016\/j.surfin.2020.100818","article-title":"Gold surface cleaning by etching polishing: Optimization of polycrystalline film topography and surface functionality for biosensing","volume":"22","author":"Snopok","year":"2021","journal-title":"Surf. Interfaces"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"e01799","DOI":"10.1002\/brb3.1799","article-title":"A lab-on-a-chip model of glaucoma","volume":"10","author":"Nafian","year":"2020","journal-title":"Brain Behav."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/18\/8257\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T16:09:32Z","timestamp":1789574972000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/18\/8257"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,16]]},"references-count":76,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["ijms27188257"],"URL":"https:\/\/doi.org\/10.3390\/ijms27188257","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,16]]}}}