{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T17:09:34Z","timestamp":1790096974829,"version":"4.0.1"},"reference-count":28,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T00:00:00Z","timestamp":1789862400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Polish Ministry of Science and Higher Education","id":[{"id":"https:\/\/ror.org\/05dwvd537","id-type":"ROR","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/05dwvd537","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Financial Support System for Scientists and Research Teams at the Warsaw University of Life Sciences","award":["853-2-80-45-780400-S25009"],"award-info":[{"award-number":["853-2-80-45-780400-S25009"]}],"id":[{"id":"https:\/\/ror.org\/05srvzs48","id-type":"ROR","asserted-by":"crossref"}]},{"award":["853-2-80-45-780400-S25009"],"award-info":[{"award-number":["853-2-80-45-780400-S25009"]}],"id":[{"id":"https:\/\/ror.org\/05srvzs48","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Sucralose is a widely consumed non-nutritive sweetener, with approximately 85% reaching the colon unmetabolized and directly interacting with the gut microbiota. Despite regulatory approval, its impact on microbial ecology and metabolic outcomes remains inconclusive. Previous meta-analyses included only two human studies and did not integrate microbiota, short-chain fatty acids, and metabolic parameters. This systematic review and meta-analysis evaluated the effects of sucralose on gut microbiota composition, alpha diversity, short-chain fatty acid production, and metabolic parameters in healthy adults based on randomized controlled trial evidence. A systematic search of PubMed, Scopus, and Google Scholar was conducted in May 2026 following PRISMA guidelines. Risk of bias was assessed using Cochrane RoB 2.0, and meta-analysis employed a REML random-effects model. Ten randomized controlled trials involving 8\u201361 participants per study (total across intervention and control groups) met the inclusion criteria; seven provided data for meta-analysis. Sucralose did not significantly alter alpha diversity (Shannon index: SMD = \u22120.00; 95% CI \u22121.16 to 1.15; p = 0.99; I2 = 25.67%), fasting blood glucose (SMD = \u22120.04; 95% CI = \u22120.63 to 0.56; p = 0.88; I2 = 69.61%), and HOMA-IR (SMD = \u22120.13; 95% CI = \u22120.36 to 0.10; p = 0.212; I2 = 0.00%). Butyrate showed a numerical decrease, although the pooled effect was not statistically significant and substantial heterogeneity was observed. Overall, the available evidence from short-term randomized controlled trials did not demonstrate statistically significant effects of sucralose consumption on the evaluated microbiota and metabolic outcomes. Standardized, longer-duration randomized controlled trials with inert comparators are needed.<\/jats:p>","DOI":"10.3390\/ijms27188377","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T13:32:12Z","timestamp":1790083932000},"page":"8377","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of Sucralose Consumption on Gut Microbiota: A Systematic Review and Meta-Analysis of Randomized Controlled Trials"],"prefix":"10.3390","volume":"27","author":[{"given":"Dwi Tari","family":"Wulandari","sequence":"first","affiliation":[{"name":"Division of Food Science and Technology, Faculty of Engineering and Technology, IPB University, IPB Dramaga Campus, Bogor 16680, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0065-6283","authenticated-orcid":false,"given":"Nuri","family":"Andarwulan","sequence":"additional","affiliation":[{"name":"Division of Food Science and Technology, Faculty of Engineering and Technology, IPB University, IPB Dramaga Campus, Bogor 16680, Indonesia"},{"name":"South-East Asia Food & Agricultural Science and Technology (SEAFAST) Center, IPB University, IPB Dramaga Campus, Bogor 16680, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Saraswati","sequence":"additional","affiliation":[{"name":"Division of Food Science and Technology, Faculty of Engineering and Technology, IPB University, IPB Dramaga Campus, Bogor 16680, Indonesia"},{"name":"South-East Asia Food & Agricultural Science and Technology (SEAFAST) Center, IPB University, IPB Dramaga Campus, Bogor 16680, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2029-3106","authenticated-orcid":false,"given":"Eny","family":"Palupi","sequence":"additional","affiliation":[{"name":"Department of Community Nutrition, Faculty of Medicine and Nutrition, IPB University, IPB Dramaga Campus, Bogor 16680, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8143-2918","authenticated-orcid":false,"given":"Dominika","family":"\u015arednicka-Tober","sequence":"additional","affiliation":[{"name":"Division of Food Science and Technology, Faculty of Engineering and Technology, IPB University, IPB Dramaga Campus, Bogor 16680, Indonesia"},{"name":"Department of Functional and Organic Food, Institute of Human Nutrition Sciences, Warsaw University of Life Sciences, Nowoursynowska 159c, 02-776 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,20]]},"reference":[{"key":"ref_1","unstructured":"Bothare, P. 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