{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T05:04:27Z","timestamp":1790139867624,"version":"4.0.1"},"reference-count":123,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Wroclaw Medical University","id":[{"id":"https:\/\/ror.org\/01qpw1b93","id-type":"ROR","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/01qpw1b93","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>This scoping review represents a comprehensive effort to conceptualize the mother, fetus, and amniotic fluid as a dynamic triad. The primary objective is to map the available literature and synthesize findings regarding how maternal mental health, psychiatric treatments, and environmental lifestyle exposures (specifically smoking) affect the biochemical composition of amniotic fluid in the context of metabolic programming. A systematic search was conducted using PubMed, Web of Science, and Embase in accordance with PRISMA Extension for Scoping Reviews guidelines. The quality (using the Quality Assessment Tool for Observational Cohort and Cross-Sectional Studies designed by the NIH) of all 30 original articles was assessed, and data regarding pregnancy and amniotic fluid findings were extracted for narrative synthesis. Chronic stress and maternal anxiety positively correlate with cortisol and corticotropin-releasing hormone levels in amniotic fluid. Psychiatric treatment with selective serotonin reuptake inhibitors was linked to elevated neuronal injury markers, such as S100B and activin A. Furthermore, smoking during pregnancy interferes with amniotic metabolic pathways, including those related to oxidative stress. The available evidence, despite limitations in examining amniotic fluid and the challenges of scientifically measuring maternal stress, supports the role of a dynamic triad between the mother, amniotic fluid, and the fetus and its interface in shaping fetal development and long-term health outcomes. Changes at the biochemical, hormonal, and genetic levels in amniotic fluid composition support the concept of metabolic programming. Understanding these pathways is crucial for recognizing early influences on fetal development and optimizing long-term health outcomes.<\/jats:p>","DOI":"10.3390\/ijms27188434","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T13:32:12Z","timestamp":1790083932000},"page":"8434","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Stressed, Stained, and Stored: The Impact of Maternal Distress and Smoking on Amniotic Biomarkers\u2014A Scoping Review"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-5379-7306","authenticated-orcid":false,"given":"Krystian","family":"Skowron","sequence":"first","affiliation":[{"name":"Scientific Association of Medical Chemistry and Immunochemistry, Wroclaw Medical University, 50-369 Wroclaw, Poland"},{"name":"Student Scientific Association for Children\u2019s Surgery, Wroclaw Medical University, 50-369 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filip","family":"Godziszewski","sequence":"additional","affiliation":[{"name":"Clinical and Dissecting Anatomy Students\u2019 Scientific Club, Wroclaw Medical University, 50-369 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Putyrska","sequence":"additional","affiliation":[{"name":"Student Scientific Association for Children\u2019s Surgery, Wroclaw Medical University, 50-369 Wroclaw, Poland"},{"name":"Student Scientific Association of Neurology, Wroclaw Medical University, 50-369 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicja","family":"\u0141yczkowska","sequence":"additional","affiliation":[{"name":"Student Scientific Association for Children\u2019s Surgery, Wroclaw Medical University, 50-369 Wroclaw, Poland"},{"name":"Student Scientific Association of Neurology, Wroclaw Medical University, 50-369 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikodem","family":"Skrzypiec","sequence":"additional","affiliation":[{"name":"Student Scientific Association for Children\u2019s Surgery, Wroclaw Medical University, 50-369 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0601-1232","authenticated-orcid":false,"given":"Magdalena","family":"Orczyk-Pawi\u0142owicz","sequence":"additional","affiliation":[{"name":"Division of Chemistry and Immunochemistry, Department of Biochemistry and Immunochemistry, Wroclaw Medical University, 50-369 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Fischer, R.L. (2008). Amniotic Fluid: Physiology and Assessment. The Global Library of Women\u2019s Medicine, Sapiens Publishing.","DOI":"10.3843\/GLOWM.10208"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"ten Broek, C.M.A., Bots, J., Varela-Lasheras, I., Bugiani, M., Galis, F., and Van Dongen, S. (2013). Amniotic Fluid Deficiency and Congenital Abnormalities Both Influence Fluctuating Asymmetry in Developing Limbs of Human Deceased Fetuses. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0081824"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/S1726-4901(09)70389-2","article-title":"Potential Function of Amniotic Fluid in Fetal Development\u2014Novel Insights by Comparing the Composition of Human Amniotic Fluid with Umbilical Cord and Maternal Serum at Mid and Late Gestation","volume":"72","author":"Tong","year":"2009","journal-title":"J. Chin. Med. Assoc."},{"key":"ref_4","first-page":"627","article-title":"Amniotic Fluid Characteristics and Its Application in Stem Cell Therapy: A Review","volume":"20","author":"Shamsnajafabadi","year":"2022","journal-title":"Int. J. Reprod. Biomed."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1038\/aps.2011.59","article-title":"Maternal-Fetal Fluid Balance and Aquaporins: From Molecule to Physiology","volume":"32","author":"Sha","year":"2011","journal-title":"Acta Pharmacol. Sin."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"17039","DOI":"10.1038\/s41598-023-44125-3","article-title":"The Amniotic Fluid Proteome Changes across Gestation in Humans and Rhesus Macaques","volume":"13","author":"Crosland","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1007\/BF01600209","article-title":"Ultrastructural Anomalies in the Fetal Small Intestine Indicate That Fetal Swallowing Is Important for Normal Development: An Experimental Study","volume":"420","author":"Trahair","year":"1992","journal-title":"Virchows Arch. A Pathol. Anat. Histopathol."},{"key":"ref_8","first-page":"524","article-title":"Trophic Effect of Multiple Growth Factors in Amniotic Fluid or Human Milk on Cultured Human Fetal Small Intestinal Cells","volume":"34","author":"Hirai","year":"2002","journal-title":"J. Pediatr. Gastroenterol. Nutr."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e88","DOI":"10.1542\/peds.107.6.e88","article-title":"Prenatal and Postnatal Flavor Learning by Human Infants","volume":"107","author":"Mennella","year":"2001","journal-title":"Pediatrics"},{"key":"ref_10","first-page":"101","article-title":"An Evaluation of Antibacterial Effects of Human Amniotic Fluid on Pathogenic and Probiotic Bacteria In Vitro","volume":"24","author":"Ghanbarzadeh","year":"2023","journal-title":"J. Reprod. Infertil."},{"key":"ref_11","first-page":"e36986","article-title":"Utilizing Amniotic Fluid Metabolomics to Monitor Fetal Well-Being: A Narrative Review of the Literature","volume":"15","author":"Kolvatzis","year":"2023","journal-title":"Cureus"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Buhimschi, C.S., Bhandari, V., Hamar, B.D., Bahtiyar, M.-O., Zhao, G., Sfakianaki, A.K., Pettker, C.M., Magloire, L., Funai, E., and Norwitz, E.R. (2007). Proteomic Profiling of the Amniotic Fluid to Detect Inflammation, Infection, and Neonatal Sepsis. PLoS Med., 4.","DOI":"10.1371\/journal.pmed.0040018"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2787","DOI":"10.19082\/2787","article-title":"The Effect of Diagnostic Amniocentesis and Its Complications on Early Spontaneous Abortion","volume":"8","author":"Tara","year":"2016","journal-title":"Electron. Physician"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Wolder, D., Blazuk-Fortak, A., Michalska, A., Detka, K., \u015awiercz, G., and Kaczmarek, P. (2025). Perinatal and Delivery Outcomes Following Amniocentesis: A Case-Control Study in the Polish Population. J. Clin. Med., 14.","DOI":"10.3390\/jcm14020309"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Sano, M., Nagura, H., Ueno, S., and Nakashima, A. (2021). Amino Acid Composition of Amniotic Fluid during the Perinatal Period Reflects Mother\u2019s Fat and Carbohydrate Intake. Nutrients, 13.","DOI":"10.3390\/nu13072136"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Visiedo, F., V\u00e1zquez-Fonseca, L., \u00c1balos-Mart\u00ednez, J., Broull\u00f3n-Molanes, J.R., Quintero-Prado, R., Mateos, R.M., and Bugatto, F. (2023). Maternal Elevated Inflammation Impairs Placental Fatty Acids \u03b2-Oxidation in Women with Gestational Diabetes Mellitus. Front. Endocrinol., 14.","DOI":"10.3389\/fendo.2023.1146574"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"O\u2019Neill, K., Alexander, J., Azuma, R., Xiao, R., Snyder, N.W., Mesaros, C.A., Blair, I.A., and Pinney, S.E. (2018). Gestational Diabetes Alters the Metabolomic Profile in 2nd Trimester Amniotic Fluid in a Sex-Specific Manner. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19092696"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Gaccioli, F., and Lager, S. (2016). Placental Nutrient Transport and Intrauterine Growth Restriction. Front. Physiol., 7.","DOI":"10.3389\/fphys.2016.00040"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1017\/S2040174412000529","article-title":"Placental Transport in Response to Altered Maternal Nutrition","volume":"4","author":"Gaccioli","year":"2013","journal-title":"J. Dev. Orig. Health Dis."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Skowron, K., Lichocki, I., Godziszewski, F., and Orczyk-Pawi\u0142owicz, M. (2025). From Mind to Milk: The Influence of Psychological Factors on the Composition of Human Breast Milk. Nutrients, 17.","DOI":"10.3390\/nu17061093"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"107308","DOI":"10.1016\/j.ntt.2023.107308","article-title":"Amniotic Fluid Cortisol Predicts Neonatal and Infant Development In-Stressed Rhesus Monkeys: Implications for Prenatal Stress","volume":"100","author":"Otridge","year":"2023","journal-title":"Neurotoxicol. Teratol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1037\/0033-2909.133.1.25","article-title":"If It Goes up, Must It Come down? Chronic Stress and the Hypothalamic-Pituitary-Adrenocortical Axis in Humans","volume":"133","author":"Miller","year":"2007","journal-title":"Psychol. Bull."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.psyneuen.2016.03.012","article-title":"The Temporal Dynamics of Cortisol and Affective States in Depressed and Non-Depressed Individuals","volume":"69","author":"Booij","year":"2016","journal-title":"Psychoneuroendocrinology"},{"key":"ref_24","first-page":"101571","article-title":"A Review on Linking Stress, Depression, and Insulin Resistance via Low-Grade Chronic Inflammation","volume":"36","author":"Mehdi","year":"2023","journal-title":"Biochem. Biophys. Rep."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"89","DOI":"10.31887\/DCNS.2011.13.1\/smbrody","article-title":"New Insights into Perinatal Depression: Pathogenesis and Treatment during Pregnancy and Postpartum","volume":"13","year":"2011","journal-title":"Dialogues Clin. Neurosci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1176\/appi.ajp.163.1.145","article-title":"Antidepressants in Amniotic Fluid: Another Route of Fetal Exposure","volume":"163","author":"Loughhead","year":"2006","journal-title":"Am. J. Psychiatry"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1186\/s12992-017-0237-5","article-title":"The Role of Unhealthy Lifestyles in the Incidence and Persistence of Depression: A Longitudinal General Population Study in Four Emerging Countries","volume":"13","author":"Cabello","year":"2017","journal-title":"Glob. Health"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1038\/jp.2012.57","article-title":"Prenatal Alcohol Exposure, Blood Alcohol Concentrations and Alcohol Elimination Rates for the Mother, Fetus and Newborn","volume":"32","author":"Burd","year":"2012","journal-title":"J. Perinatol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1763","DOI":"10.1093\/ntr\/ntab080","article-title":"Nicotine Induces Maternal and Fetal Inflammatory Responses Which Predispose Intrauterine Infection Risk in a Rat Model","volume":"23","author":"Reyes","year":"2021","journal-title":"Nicotine Tob. Res."},{"key":"ref_30","first-page":"e51534","article-title":"TORCH (Toxoplasmosis, Other, Rubella, Cytomegalovirus, Herpes Simplex Virus) Infection and the Enigma of Anomalous Fetal Development: Pregnancy Puzzles","volume":"16","author":"Patel","year":"2024","journal-title":"Cureus"},{"key":"ref_31","unstructured":"National Heart, Lung, and Blood Institute (2026, September 03). Study Quality Assessment Tools, Available online: https:\/\/www.nhlbi.nih.gov\/health-topics\/study-quality-assessment-tools."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"n71","DOI":"10.1136\/bmj.n71","article-title":"The PRISMA 2020 Statement: An Updated Guideline for Reporting Systematic Reviews","volume":"372","author":"Page","year":"2021","journal-title":"BMJ"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"107256","DOI":"10.1016\/j.psyneuen.2024.107256","article-title":"Does High Perceived Stress over the Past Month Alter Cortisol Reactivity to the Trier Social Stress Test?","volume":"172","author":"Creswell","year":"2025","journal-title":"Psychoneuroendocrinology"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1002\/dev.21007","article-title":"Prenatal Cortisol Exposure Predicts Infant Cortisol Response to Acute Stress","volume":"55","author":"Bergman","year":"2013","journal-title":"Dev. Psychobiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1016\/j.biopsych.2010.01.002","article-title":"Maternal Prenatal Cortisol and Infant Cognitive Development: Moderation by Infant-Mother Attachment","volume":"67","author":"Bergman","year":"2010","journal-title":"Biol. Psychiatry"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1016\/j.psyneuen.2008.10.005","article-title":"Association between Maternal and Amniotic Fluid Cortisol Is Moderated by Maternal Anxiety","volume":"34","author":"Glover","year":"2009","journal-title":"Psychoneuroendocrinology"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1016\/j.psyneuen.2012.09.015","article-title":"Influence of Prenatal Maternal Stress, Maternal Plasma Cortisol and Cortisol in the Amniotic Fluid on Birth Outcomes and Child Temperament at 3 Months","volume":"38","author":"Baibazarova","year":"2013","journal-title":"Psychoneuroendocrinology"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1111\/j.1365-2826.2008.01659.x","article-title":"Maternal Antenatal Anxiety and Amniotic Fluid Cortisol and Testosterone: Possible Implications for Foetal Programming","volume":"20","author":"Sarkar","year":"2008","journal-title":"J. Neuroendocrinol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1111\/j.1365-2265.2007.02785.x","article-title":"Ontogeny of Foetal Exposure to Maternal Cortisol Using Midtrimester Fluid as a Biomarker","volume":"66","author":"Sarkar","year":"2007","journal-title":"Clin. Endocrinol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1002\/uog.29238","article-title":"Maternal Stress and Fetoplacental Cortisol Regulation in Small-for-Gestational-Age Newborns","volume":"65","author":"Miranda","year":"2025","journal-title":"Ultrasound Obstet. Gynecol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1111\/j.1365-2265.2007.02955.x","article-title":"Amniotic Fluid Testosterone: Relationship with Cortisol and Gestational Age","volume":"67","author":"Sarkar","year":"2007","journal-title":"Clin. Endocrinol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/j.yhbeh.2009.12.012","article-title":"In Utero Cortisol and Testosterone Exposure and Fear Reactivity In","volume":"57","author":"Bergman","year":"2010","journal-title":"Horm. Behav."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Ilkevi\u010d, E., Hausmann, M., and Grik\u0161ien\u0117, R. (2024). Emotion Recognition and Regulation in Males: Role of Sex and Stress Steroids. Front. Neuroendocrinol., 74.","DOI":"10.1016\/j.yfrne.2024.101145"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1017\/S0954579418000147","article-title":"Cortisol, Cortisone, and BDNF in Amniotic Fluid in the Second Trimester of Pregnancy: Effect of Early Life and Current Maternal Stress and Socioeconomic Status","volume":"30","author":"Deuschle","year":"2018","journal-title":"Dev. Psychopathol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1002\/dev.21142","article-title":"The Association between the Acute Psychobiological Stress Response in Second Trimester Pregnant Women, Amniotic Fluid Glucocorticoids, and Neonatal Birth Outcome","volume":"56","author":"Ghaemmaghami","year":"2014","journal-title":"Dev. Psychobiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1097\/PSY.0000000000000130","article-title":"The Acute Autonomic Stress Response and Amniotic Fluid Glucocorticoids in Second-Trimester Pregnant Women","volume":"77","author":"Dainese","year":"2015","journal-title":"Psychosom. Med."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1080\/10253890.2017.1312336","article-title":"Second-Trimester Amniotic Fluid Corticotropin-Releasing Hormone and Urocortin in Relation to Maternal Stress and Fetal Growth in Human Pregnancy","volume":"20","author":"Dainese","year":"2017","journal-title":"Stress"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1002\/uog.138","article-title":"Maternal Anxiety and Fetal Behavior at 15 Weeks\u2019 Gestation","volume":"22","author":"Bartha","year":"2003","journal-title":"Ultrasound Obstet. Gynecol."},{"key":"ref_49","first-page":"253","article-title":"Catecholamines in Amniotic Fluid as Indicators of Intrapartum Fetal Stress","volume":"39","author":"Yashiro","year":"1985","journal-title":"Acta Med. Okayama"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"100658","DOI":"10.1016\/j.ynstr.2024.100658","article-title":"Stress in Pregnancy\u2014Implications for Fetal BDNF in Amniotic Fluid at Birth","volume":"31","author":"Lindner","year":"2024","journal-title":"Neurobiol. Stress"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"8476217","DOI":"10.1155\/2018\/8476217","article-title":"Association between Brain-Derived Neurotrophic Factor (BDNF) Levels in 2nd Trimester Amniotic Fluid and Fetal Development","volume":"2018","author":"Antonakopoulos","year":"2018","journal-title":"Mediat. Inflamm."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"579","DOI":"10.3892\/mmr.2014.2258","article-title":"Insulin-like Growth Factors in Embryonic and Fetal Growth and Skeletal Development (Review)","volume":"10","author":"Agrogiannis","year":"2014","journal-title":"Mol. Med. Rep."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"105180","DOI":"10.1016\/j.psyneuen.2021.105180","article-title":"Childhood Trauma and Insulin-like Growth Factors in Amniotic Fluid An Exploratory Analysis of 79 Women","volume":"127","author":"Lamade","year":"2021","journal-title":"Psychoneuroendocrinology"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1176\/appi.ajp.160.5.993","article-title":"Placental Passage of Antidepressant Medications","volume":"160","author":"Hendrick","year":"2003","journal-title":"Am. J. Psychiatry"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"F134","DOI":"10.1136\/fn.84.2.F134","article-title":"Neonatal Paroxetine Withdrawal Syndrome","volume":"84","author":"Stiskal","year":"2001","journal-title":"Arch. Dis. Child. Fetal Neonatal Ed."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"49","DOI":"10.2174\/1871527314666150116114033","article-title":"Antenatal Maternal Antidepressants Drugs Affect S100B Concentrations in Fetal-Maternal Biological Fluids","volume":"14","author":"Bellissima","year":"2015","journal-title":"CNS Neurol. Disord. Drug Targets"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.cca.2019.11.027","article-title":"Antenatal Maternal Antidepressants Drugs Treatment Affects S100B Levels in Maternal-Fetal Biological Fluids in a Dose Dependent Manner","volume":"501","author":"Bellissima","year":"2020","journal-title":"Clin. Chim. Acta"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"32","DOI":"10.3109\/14767058.2011.604931","article-title":"Antenatal Maternal Antidepressants Drugs Affect Activin A concentrations in Maternal Blood, in Amniotic Fluid and in Fetal Cord Blood","volume":"24","author":"Bellissima","year":"2011","journal-title":"J. Matern.-Fetal Neonatal Med."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1111\/ppc.12174","article-title":"Are Major Depression and Generalized Anxiety Disorder Associated With Oligohydramnios in Pregnant Women? A Case-Control Study","volume":"53","author":"Uguz","year":"2017","journal-title":"Perspect. Psychiatr. Care"},{"key":"ref_60","first-page":"813","article-title":"Influence of Maternal Smoking during Pregnancy on Oxidant Status in Amniotic Fluid","volume":"26","author":"Dittrich","year":"2012","journal-title":"In Vivo"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.envint.2017.07.019","article-title":"Low-Level Maternal Exposure to Nicotine Associates with Significant Metabolic Perturbations in Second-Trimester Amniotic Fluid","volume":"107","author":"Fischer","year":"2017","journal-title":"Environ. Int."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.ejogrb.2012.07.031","article-title":"Second Trimester Amniotic Fluid Adiponectin Level Is Affected by maternal Tobacco Exposure, Insulin, and PAPP-A Level","volume":"165","author":"Perez","year":"2012","journal-title":"Eur. J. Obstet. Gynecol. Reprod. Biol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"360","DOI":"10.2174\/157019311797440317","article-title":"The Role of Oxidative Stress in Smoking-Related Diseases","volume":"8","author":"Fearon","year":"2011","journal-title":"Mini. Rev. Org. Chem."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"601","DOI":"10.3109\/10715769809065816","article-title":"The Lag Phase","volume":"28","author":"Cadenas","year":"1998","journal-title":"Free Radic. Res."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1161\/HYPERTENSIONAHA.109.130583","article-title":"Circulating and Placental Growth-Differentiation Factor 15 in Preeclampsia and in Pregnancy Complicated by Diabetes Mellitus","volume":"54","author":"Sugulle","year":"2009","journal-title":"Hypertension"},{"key":"ref_66","first-page":"199","article-title":"Neuron Specific Enolase in Amniotic Fluid\u2014A Possible Indicator for Fetal Distress and Brain Implication","volume":"15","author":"Zinsmeyer","year":"1987","journal-title":"J. Perinat. Med."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"251","DOI":"10.3109\/01443615.2013.851656","article-title":"Decrease in 3-Nitrotyrosine in the Amniotic Fluid of Women with Cytomegalovirus Infection","volume":"34","author":"Gaj","year":"2014","journal-title":"J. Obstet. Gynaecol."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Edlow, A.G., Vora, N.L., Hui, L., Wick, H.C., Cowan, J.M., and Bianchi, D.W. (2014). Maternal Obesity Affects Fetal Neurodevelopmental and Metabolic Gene: A Pilot Study. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0088661"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"910","DOI":"10.3109\/14767058.2014.937692","article-title":"Second Trimester Amniotic Fluid Glucose, Uric Acid, Phosphate, Potassium, and Sodium Concentrations in Relation to Maternal Pre-Pregnancy BMI and Birth Weight Centiles","volume":"28","author":"Fotiou","year":"2015","journal-title":"J. Matern.-Fetal Neonatal Med."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Fl\u00f6ck, A., Odainic, A., Dolscheid-Pommerich, R., Jaskolski, M.R., Diedrich, A.K., Puth, M.T., Schmidt, S., Stoffel-Wagner, B., Strizek, B., and Gembruch, U. (2022). High Maternal BMI and Low Maternal Blood BDNF May Determine the Limit of Detection of Amniotic Fluid BDNF throughout Gestation: Analysis of Mother-Fetus Trios and Literature Review. PLoS ONE, 17.","DOI":"10.1371\/journal.pone.0265186"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"e002765","DOI":"10.1136\/bmjph-2025-002765","article-title":"Disruption and Recovery in Physical and Mental Health, Body Mass Index and Smoking during the COVID-19 Pandemic: A Trend Analysis of US BRFSS Data from 2016 to 2022","volume":"3","author":"Balsara","year":"2025","journal-title":"BMJ Public Health"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1001\/jamapsychiatry.2018.4543","article-title":"Association of Early-Life Stress With Cytomegalovirus Infection in Adults With Major Depressive Disorder","volume":"76","author":"Ford","year":"2019","journal-title":"JAMA Psychiatry"},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Gale, S.D., Farrer, T.J., Erbstoesser, R., MacLean, S., and Hedges, D.W. (2024). Human Cytomegalovirus Infection and Neurocognitive and Neuropsychiatric Health. Pathogens, 13.","DOI":"10.3390\/pathogens13050417"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"e28782","DOI":"10.1016\/j.heliyon.2024.e28782","article-title":"The Risk Factors for Mental Health Disorders in Patients with Type 2 Diabetes: An Umbrella Review of Systematic Reviews with and without Meta-Analysis","volume":"10","author":"Busili","year":"2024","journal-title":"Heliyon"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1097\/CM9.0000000000003230","article-title":"Oxidative Stress in Diabetes Mellitus and Its Complications: From Pathophysiology to Therapeutic Strategies","volume":"138","author":"Chen","year":"2025","journal-title":"Chin. Med. J."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"246","DOI":"10.3390\/oxygen2030018","article-title":"Roles of Reactive Oxygen Species in Vascular Complications of Diabetes: Therapeutic Properties of Medicinal Plants and Food","volume":"2","author":"Tan","year":"2022","journal-title":"Oxygen"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1016\/S0002-9378(99)70110-9","article-title":"Elevated Level of Free 8-Iso-Prostaglandin F2\u03b1 in the Decidua Basalis of Women with Preeclampsia","volume":"181","author":"Staff","year":"1999","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1096\/fasebj.14.10.1289","article-title":"Placental Isoprostane Is Significantly Increased in Preeclampsia","volume":"14","author":"Walsh","year":"2000","journal-title":"FASEB J."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"1991","DOI":"10.1038\/oby.2005.244","article-title":"Major Depression Predicts an Increase in Long-Term Body Weight Variability in Young Adults","volume":"13","author":"Hasler","year":"2005","journal-title":"Obes. Res."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"437","DOI":"10.31887\/DCNS.2016.18.4\/cepperson","article-title":"Considering Sex and Gender in Alzheimer Disease and Other Dementias","volume":"18","author":"Podcasy","year":"2016","journal-title":"Dialogues Clin. Neurosci."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1392","DOI":"10.1016\/j.humimm.2013.06.009","article-title":"3-Nitrotyrosine: A Biomarker of Nitrogen Free Radical Species Modified Proteins in Systemic Autoimmunogenic Conditions","volume":"74","author":"Ahsan","year":"2013","journal-title":"Hum. Immunol."},{"key":"ref_82","first-page":"642","article-title":"Psychological Aspects of Pain Experience in Amniocentesis","volume":"110","author":"Dorfer","year":"1998","journal-title":"Wien. Klin. Wochenschr."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1002\/pd.1444","article-title":"Maternal Anxiety at Amniocentesis and Plasma Cortisol","volume":"26","author":"Sarkar","year":"2006","journal-title":"Prenat. Diagn."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"137","DOI":"10.3325\/cmj.2010.51.137","article-title":"Stress and Anxiety in Relation to Amniocentesis: Do Women Who Perceive Their Partners to Be More Involved in Pregnancy Feel Less Stressed and Anxious?","volume":"51","year":"2010","journal-title":"Croat. Med. J."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"112","DOI":"10.18051\/UnivMed.2015.v34.112-120","article-title":"Amniocentesis Increases Level of Anxiety in Women with Invasive Prenatal Diagnosis of Down Syndrome","volume":"34","author":"Tursinawati","year":"2015","journal-title":"Universa Med."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1177\/1099800420942893","article-title":"Associations Between Hormonal Biomarkers and Preterm Infant Health and Development During the First 2 Years After Birth","volume":"23","author":"Cho","year":"2021","journal-title":"Biol. Res. Nurs."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"106341","DOI":"10.1016\/j.psyneuen.2023.106341","article-title":"Differential Susceptibility to Parenting Influences on Reactive and Proactive Aggression: The Role of Testosterone and Cortisol in Children","volume":"155","author":"Carreras","year":"2023","journal-title":"Psychoneuroendocrinology"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"102051","DOI":"10.1016\/j.avb.2025.102051","article-title":"A Systematic Review of the Relationship between Cortisol, Testosterone, and Aggression in Children and Adolescents","volume":"82","author":"Cima","year":"2025","journal-title":"Aggress. Violent Behav."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1348\/000712608X311731","article-title":"Fetal Testosterone and Autistic Traits","volume":"100","author":"Auyeung","year":"2009","journal-title":"Br. J. Psychol."},{"key":"ref_90","first-page":"e30898","article-title":"Risk Factors of Postpartum Depression","volume":"14","author":"Agrawal","year":"2022","journal-title":"Cureus"},{"key":"ref_91","doi-asserted-by":"crossref","unstructured":"Howard, K., Maples, J.M., and Tinius, R.A. (2022). Modifiable Maternal Factors and Their Relationship to Postpartum Depression. Int. J. Environ. Res. Public Health, 19.","DOI":"10.3390\/ijerph191912393"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"816","DOI":"10.4161\/epi.28703","article-title":"Epigenetic Regulation of the Placental HSD11B2 Barrier and Its Role as a Critical Regulator of Fetal Development","volume":"9","author":"Togher","year":"2014","journal-title":"Epigenetics"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1093\/humrep\/13.4.799","article-title":"11Beta-Hydroxysteroid Dehydrogenase Type 2 in Human Pregnancy and Reduced Expression in Intrauterine Growth Restriction","volume":"13","author":"Shams","year":"1998","journal-title":"Hum. Reprod."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"jeb246073","DOI":"10.1242\/jeb.246073","article-title":"How Is Prenatal Stress Transmitted from the Mother to the Fetus?","volume":"227","author":"Sze","year":"2024","journal-title":"J. Exp. Biol."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.1172\/JCI21647","article-title":"Epigenetic Regulation of 11\u03b2-Hydroxysteroid Dehydrogenase Type 2 Expression","volume":"114","author":"Fouladkou","year":"2004","journal-title":"J. Clin. Investig."},{"key":"ref_96","doi-asserted-by":"crossref","unstructured":"Jensen Pe\u00f1a, C., Monk, C., and Champagne, F.A. (2012). Epigenetic Effects of Prenatal Stress on 11\u03b2-Hydroxysteroid Dehydrogenase-2 in the Placenta and Fetal Brain. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0039791"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.placenta.2020.12.008","article-title":"Maternal Stress, Placental 11\u03b2-Hydroxysteroid Dehydrogenase Type 2, and Infant HPA Axis Development in Humans: Psychosocial and Physiological Pathways","volume":"104","author":"Jahnke","year":"2021","journal-title":"Placenta"},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1038\/npp.2015.231","article-title":"The Placenta as a Mediator of Stress Effects on Neurodevelopmental Reprogramming","volume":"41","author":"Bronson","year":"2016","journal-title":"Neuropsychopharmacology"},{"key":"ref_99","doi-asserted-by":"crossref","unstructured":"Pavli, P., Mastorakos, G., Eleftheriades, M., and Valsamakis, G. (2025). The Effect of Maternal Stress on 11beta-Hydroxysteroid Dehydrogenase Activity During Pregnancy: Evidence for Potential Pregnancy Complications and Consequences on Fetal Development and Metabolism. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms262211071"},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"27482","DOI":"10.3390\/ijms161126034","article-title":"Maternal Prenatal Mental Health and Placental 11\u03b2-HSD2 Gene Expression: Initial Findings from the Mercy Pregnancy and Emotional Wellbeing Study","volume":"16","author":"Seth","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"4951","DOI":"10.1210\/jc.2010-1215","article-title":"Salivary Cortisone Is a Potential Biomarker for Serum Free Cortisol","volume":"95","author":"Perogamvros","year":"2010","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_102","doi-asserted-by":"crossref","unstructured":"Lugovi\u0107-Mihi\u0107, L., \u0160trajtenberger, M., Kuna, M., Ladika-Davidovi\u0107, B., Barac, E., and Vilibi\u0107, M. (2025). Association Between Proinflammatory Cytokines IL-6 and TNF-Alpha, Psychological Stress and Chronic Spontaneous Urticaria Severity. Int. J. Mol. Sci., 26.","DOI":"10.20944\/preprints202508.1087.v1"},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.bbi.2019.06.001","article-title":"Effects of SSRIs on Peripheral Inflammatory Cytokines in Patients with Generalized Anxiety Disorder","volume":"81","author":"Hou","year":"2019","journal-title":"Brain Behav. Immun."},{"key":"ref_104","unstructured":"Sylv\u00e9n, S. (2012). Biological and Psychosocial Aspects of Postpartum Depression. [Ph.D. Thesis, Uppsala University]."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"e2453332","DOI":"10.1001\/jamanetworkopen.2024.53332","article-title":"Macronutrients in Human Milk Exposed to Antidepressant and Anti-Inflammatory Medications","volume":"8","author":"Bertrand","year":"2025","journal-title":"JAMA Netw. Open"},{"key":"ref_106","doi-asserted-by":"crossref","unstructured":"Alwan, S., and Berard, A. (2025). Epidemiology of Use of Psychotropic Drugs in Pregnant and Nursing Women. Perinatal Psychopharmacology, Springer Nature.","DOI":"10.1007\/978-3-031-99720-4_1"},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"F472","DOI":"10.1136\/archdischild-2011-214239","article-title":"Maternal Use of SSRIs, SNRIs and NaSSAs: Practical Recommendations during Pregnancy and Lactation","volume":"97","author":"Sie","year":"2012","journal-title":"Arch. Dis. Child. Fetal Neonatal Ed."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"245","DOI":"10.12740\/PP\/103385","article-title":"Recommendations of the Polish Psychiatric Association for Treatment of Affective Disorders in Women of Childbearing Age. Part I: Treatment of Depression","volume":"53","author":"Samochowiec","year":"2019","journal-title":"Psychiatr. Pol."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1177\/1753495X13495194","article-title":"Selective Serotonin Reuptake Inhibitors and Pregnancy: A Review of Maternal, Fetal and Neonatal Risks and Benefits","volume":"6","author":"Marchocki","year":"2013","journal-title":"Obstet. Med."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/S0006-8993(99)02430-0","article-title":"Comparative Study of Fluoxetine, Sibutramine, Sertraline and Dexfenfluramine on the Morphology of Serotonergic Nerve Terminals Using Serotonin Immunohistochemistry","volume":"858","author":"Kalia","year":"2000","journal-title":"Brain Res."},{"key":"ref_111","doi-asserted-by":"crossref","unstructured":"Hosseini, K., Cediel-Ulloa, A., AL-Sabri, M.H., Forsby, A., and Fredriksson, R. (2024). Assessing the Neurodevelopmental Impact of Fluoxetine, Citalopram, and Paroxetine on Neural Stem Cell-Derived Neurons. Pharmaceuticals, 17.","DOI":"10.3390\/ph17101392"},{"key":"ref_112","doi-asserted-by":"crossref","unstructured":"Derme, M., Fiore, M., Piccioni, M.G., Denotti, M., D\u2019Ambrosio, V., Francati, S., Mappa, I., and Rizzo, G. (2025). Smoking and Alcohol During Pregnancy: Effects on Fetal and Neonatal-A Pilot Study. J. Clin. Med., 14.","DOI":"10.3390\/jcm14197023"},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1159\/000028231","article-title":"Formation and Retention of Cotinine during Placental Transfer of Nicotine in Human Placental Cotyledon","volume":"57","author":"Sastry","year":"1998","journal-title":"Pharmacology"},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1080\/01443615.2019.1599834","article-title":"The Effects of Amniotic Fluid and Foetal Cord Blood Cotinine Concentrations on Pregnancy Complications and the Anthropometric Measurements of Newborns","volume":"39","author":"Terzioglu","year":"2019","journal-title":"J. Obstet. Gynaecol."},{"key":"ref_115","first-page":"384","article-title":"Extent of Nicotine and Cotinine Transfer to the Human Fetus, Placenta and Amniotic Fluid of Smoking Mothers","volume":"8","author":"Luck","year":"1985","journal-title":"Dev. Pharmacol. Ther."},{"key":"ref_116","first-page":"A101","article-title":"Smoking Behavior of the Mother and Sudden Infant Death Syndrome","volume":"9","author":"Vlachou","year":"2025","journal-title":"Eur. J. Midwifery"},{"key":"ref_117","doi-asserted-by":"crossref","unstructured":"\u00c1lvarez-Mej\u00eda, D., Rodas, J.A., and Leon-Rojas, J.E. (2025). From Womb to Mind: Prenatal Epigenetic Influences on Mental Health Disorders. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26136096"},{"key":"ref_118","doi-asserted-by":"crossref","unstructured":"Doi, M., Usui, N., and Shimada, S. (2022). Prenatal Environment and Neurodevelopmental Disorders. Front. Endocrinol., 13.","DOI":"10.3389\/fendo.2022.860110"},{"key":"ref_119","doi-asserted-by":"crossref","unstructured":"Wells, A.C., and Lotfipour, S. (2023). Prenatal Nicotine Exposure during Pregnancy Results in Adverse Neurodevelopmental Alterations and Neurobehavioral Deficits. Adv. Drug Alcohol Res., 3.","DOI":"10.3389\/adar.2023.11628"},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"453","DOI":"10.32641\/andespediatr.v94i4.4514","article-title":"Niveles S\u00e9ricos Bajos de Factor de Crecimiento Insul\u00ednico Tipo 1 (IGF-1) y Morbilidad En El Reci\u00e9n Nacido Prematuro","volume":"94","year":"2023","journal-title":"Andes Pediatr."},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"4076","DOI":"10.1038\/s41598-018-22230-y","article-title":"1H NMR-Based Metabolomics Reveals the Effect of Maternal Habitual Dietary Patterns on Human Amniotic Fluid Profile","volume":"8","author":"Fotiou","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"dev202088","DOI":"10.1242\/dev.202088","article-title":"Maternal-Fetal Cross-Talk via the Placenta: Influence on Offspring Development and Metabolism","volume":"150","author":"Kramer","year":"2023","journal-title":"Development"},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"a023101","DOI":"10.1101\/cshperspect.a023101","article-title":"The Amniotic Fluid Transcriptome as a Guide to Understanding Fetal Disease","volume":"5","author":"Zwemer","year":"2015","journal-title":"Cold Spring Harb. Perspect. Med."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/18\/8434\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T04:21:10Z","timestamp":1790137270000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/18\/8434"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,21]]},"references-count":123,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["ijms27188434"],"URL":"https:\/\/doi.org\/10.3390\/ijms27188434","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,21]]}}}