{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T04:58:53Z","timestamp":1790398733075,"version":"4.1.0"},"reference-count":39,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science and Technology Council","award":["NSTC 114-2314-B-182A-144"],"award-info":[{"award-number":["NSTC 114-2314-B-182A-144"]}]},{"award":["NSTC 114-2314-B-182A-144"],"award-info":[{"award-number":["NSTC 114-2314-B-182A-144"]}],"id":[{"id":"https:\/\/ror.org\/02kv4zf79","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Lipopolysaccharide (LPS) induces pulmonary fibrosis by increasing microvascular permeability and inducing severe inflammation and extracellular matrix accumulation. Mechanical ventilation (MV), although life-sustaining, can increase inflammatory cytokine production, oxidative stress, and alveolar\u2013capillary membrane permeability, leading to ventilator-induced lung injury and pulmonary fibrosis. Lumican is essential for extracellular matrix signaling and wound fibrogenesis in the lungs, cornea, and heart. Lumikine, a peptide corresponding to the 13 C-terminal amino acids of lumican, binds to transforming growth factor-\u03b2 receptor I\/activin receptor-like kinase 5 and promotes wound healing. How the lumican pathway contributes to MV-induced lung inflammation and fibrosis remains unclear. We hypothesized that MV with or without LPS pretreatment would exacerbate lung injury, oxidative stress, and lung fibrosis through the lumican pathway. C57BL\/6 mice (wild-type and lumican-deficient) were injected with LPS (intratracheal; 7 days) to induce lung fibrosis and then subjected to MV (10 mL\/kg; room air; 8 h). Nonventilated mice served as controls. MV with LPS pretreatment reduced lumican expression and increased microvascular permeability, matrix metalloproteinase-9 level, transforming growth factor-\u03b21 level, \u03b1-smooth muscle actin staining intensity, and Masson\u2019s trichrome staining intensity. Lumikine substantially mitigated these fibrotic changes. In conclusion, lumikine attenuates MV-induced exacerbation of pulmonary fibrosis after LPS-induced acute lung injury, partially by modulating the lumican pathway.<\/jats:p>","DOI":"10.3390\/ijms27198515","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T09:07:54Z","timestamp":1790240874000},"page":"8515","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Lumikine Attenuates Ventilation-Induced Lung Injury in Mice with Lipopolysaccharide-Induced Lung Fibrosis"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5819-7238","authenticated-orcid":false,"given":"Li-Fu","family":"Li","sequence":"first","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Chang Gung Memorial Hospital, Keelung 20401, Taiwan"},{"name":"Department of Internal Medicine, Chang Gung University, Taoyuan 33302, 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