{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:58:21Z","timestamp":1790744301625,"version":"4.1.0"},"reference-count":39,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012542","name":"Dalin Tzu Chi Hospital","doi-asserted-by":"publisher","award":["DTCRD101-E-02"],"award-info":[{"award-number":["DTCRD101-E-02"]}],"id":[{"id":"10.13039\/501100012542","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>GJB2 encodes connexin 26 (Cx26), and pathogenic GJB2 variants are among the most common genetic causes of hereditary hearing loss; because Cx26 is expressed in metabolically relevant tissues including liver, pancreas, and adipose tissue, whether the hearing-loss-associated c.109G&gt;A (p.V37M) variant also modifies systemic metabolic responses to high-fat diet (HFD) exposure remains unclear. Three-month-old male wild-type (WT) and homozygous Gjb2 V37M mice were assigned to low-fat diet (LFD) or high-fat diet (HFD) groups, generating four study groups: WT-LFD, WT-HFD, V37M-LFD, and V37M-HFD. The mice were followed until 12 months of age, with dietary intervention continued for 9 months. Body weight, body mass index (BMI), fasting blood glucose, serum triglycerides, and HDL cholesterol were assessed at 3, 6, 9, and 12 months. An oral glucose tolerance test was performed at 9 months, and omental fat was measured at 12 months. WT-HFD mice showed marked progressive increases in body weight and BMI, whereas increases were less pronounced in V37M-HFD mice. At 12 months, body weight and BMI did not differ significantly between V37M-LFD and V37M-HFD mice. Fasting glucose, glucose tolerance, triglyceride, and HDL cholesterol profiles differed among groups. Omental fat weight also differed significantly, although the only significant post hoc comparison was between WT-HFD and V37M-LFD mice. These findings indicate that Gjb2 V37M is associated with distinct weight, glycemic, and lipid responses during chronic dietary exposure, suggesting that GJB2 variants may modify systemic metabolic adaptation to chronic high-fat feeding.<\/jats:p>","DOI":"10.3390\/ijms27198603","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T16:31:14Z","timestamp":1790353874000},"page":"8603","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Gjb2 V37M Modifies Metabolic Responses to Chronic High-Fat Feeding in Mice"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2588-8881","authenticated-orcid":false,"given":"Cheng-Yu","family":"Hsieh","sequence":"first","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Taichung 427213, Taiwan"},{"name":"School of Medicine, Tzu Chi University, Hualien 970374, Taiwan"},{"name":"Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei 100233, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying-Chang","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, National Taiwan University Hospital, Taipei 100225, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuan-Jen","family":"Hsu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Taichung 427213, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6771-5025","authenticated-orcid":false,"given":"Tien-Chen","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, National Taiwan University Hospital, Taipei 100225, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Shiung","family":"Yang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei 100233, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5047-2204","authenticated-orcid":false,"given":"Chen-Chi","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, National Taiwan University Hospital, Taipei 100225, Taiwan"},{"name":"Department of Otolaryngology-Head and Neck Surgery, National Taiwan University Hospital Hsin-Chu Branch, Hsinchu 300195, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1873-2472","authenticated-orcid":false,"given":"Juen-Haur","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Taichung 427213, Taiwan"},{"name":"School of Medicine, Tzu Chi University, Hualien 970374, Taiwan"},{"name":"Department of Otolaryngology-Head and Neck Surgery, Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Chiayi 62247, Taiwan"},{"name":"Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 40447, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.physbeh.2004.02.006","article-title":"Genetic vulnerability to diet-induced obesity in the C57BL\/6J mouse: Physiological and molecular characteristics","volume":"81","author":"Collins","year":"2004","journal-title":"Physiol. 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