{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:19:17Z","timestamp":1790615957443,"version":"4.1.0"},"reference-count":61,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:00:00Z","timestamp":1790380800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Ovarian cancer (OC) is a leading cause of death in women, with limited advances in diagnosis and treatment over the past decade. The Synovial Sarcoma, X Breakpoint (SSX) family of cancer-testis antigens represent promising biomarkers and immunotherapeutic targets; however, the functions of individual SSX family members remain poorly defined due to their high sequence similarity. We investigated the roles of SSX2A, SSX2B and SSX3 using RNA sequencing, in vitro and in vivo assays. Pathway analysis revealed involvement in nucleosome assembly, calcium signalling, and cellular stress responses, alongside increased OC cell proliferation in vitro following overexpression. Additionally, SSX variants altered key epithelial\u2013mesenchymal transition (EMT) associated markers, including upregulation of the transcription factor, SLUG. Integration with patient transcriptomic datasets identified several SSX regulated genes associated with overall survival (OS). These findings suggest that SSX family members are not functionally redundant and may contribute to OC progression and clinical outcome.<\/jats:p>","DOI":"10.3390\/ijms27198608","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:50:54Z","timestamp":1790614254000},"page":"8608","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Distinct and Convergent Roles of the Synovial Sarcoma X Breakpoint Family of Cancer-Testis Antigens in Ovarian Cancer"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-4602-0363","authenticated-orcid":false,"given":"Kelly C.","family":"Grayson","sequence":"first","affiliation":[{"name":"Centre for Biomedicine, Hull York Medical School, University of Hull, Hull HU6 7RX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8453-7264","authenticated-orcid":false,"given":"Holly N.","family":"Wilkinson","sequence":"additional","affiliation":[{"name":"Wound Innovation Institute, University of Hull, Hull HU6 7RX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John D.","family":"Wright","sequence":"additional","affiliation":[{"name":"Centre for Biomedicine, Hull York Medical School, University of Hull, Hull HU6 7RX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen J.","family":"Archibald","sequence":"additional","affiliation":[{"name":"Centre for Biomedicine, Hull York Medical School, University of Hull, Hull HU6 7RX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J.","family":"Hardman","sequence":"additional","affiliation":[{"name":"Wound Innovation Institute, University of Hull, Hull HU6 7RX, UK"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"supervision","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0639-4541","authenticated-orcid":false,"given":"Barbara-ann","family":"Guinn","sequence":"additional","affiliation":[{"name":"Centre for Biomedicine, Hull York Medical School, University of Hull, Hull HU6 7RX, UK"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"conceptualization","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"x111","DOI":"10.1093\/annonc\/mds300","article-title":"New insights into ovarian cancer pathology","volume":"23","author":"Prat","year":"2012","journal-title":"Ann. Oncol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Lisio, M.A., Fu, L., Goyeneche, A., Gao, Z.H., and Telleria, C. (2019). High-Grade Serous Ovarian Cancer: Basic Sciences, Clinical and Therapeutic Standpoints. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20040952"},{"key":"ref_3","first-page":"760429","article-title":"Serous tubal intraepithelial carcinoma: An incidental finding at the time of prophylactic bilateral salpingo-oophorectomy","volume":"2015","author":"Vaughan","year":"2015","journal-title":"Case Rep. Obstet. Gynecol."},{"key":"ref_4","first-page":"254","article-title":"Current clinical application of serum biomarkers to detect ovarian cancer","volume":"14","author":"Nowak","year":"2015","journal-title":"Przegl\u0105d Menopauzalny"},{"key":"ref_5","first-page":"713","article-title":"Correlation of CA-125 serum level and clinico-pathological characteristic of patients with endometriosis","volume":"14","author":"Sekhavat","year":"2016","journal-title":"Int. J. Reprod. Biomed."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/j.ygyno.2008.05.006","article-title":"The epidemiology of CA-125 in women without evidence of ovarian cancer in the Prostate, Lung, Colorectal and Ovarian Cancer (PLCO) Screening Trial","volume":"110","author":"Johnson","year":"2008","journal-title":"Gynecol. Oncol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"127","DOI":"10.3109\/08830185.2011.572504","article-title":"Cancer testis antigens: Novel biomarkers and targetable proteins for ovarian cancer","volume":"30","author":"Mirandola","year":"2011","journal-title":"Int. Rev. Immunol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.semcancer.2018.08.006","article-title":"Cancer-testis antigens: Unique cancer stem cell biomarkers and targets for cancer therapy","volume":"53","author":"Gordeeva","year":"2018","journal-title":"Semin. Cancer Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1038\/ng0894-502","article-title":"Identification of novel genes, SYT and SSX, involved in the t(X;18)(p11.2;q11.2) translocation found in human synovial sarcoma","volume":"7","author":"Clark","year":"1994","journal-title":"Nat. Genet."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1002\/ijc.10634","article-title":"The SSX gene family: Characterization of 9 complete genes","volume":"101","author":"Wei","year":"2002","journal-title":"Int. J. Cancer"},{"key":"ref_11","first-page":"4766","article-title":"The SSX-2 gene, which is involved in the t(X;18) translocation of synovial sarcomas, codes for the human tumor antigen HOM-MEL-40","volume":"56","author":"Sahin","year":"1996","journal-title":"Cancer Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1002\/(SICI)1097-0215(19980703)77:1<19::AID-IJC4>3.0.CO;2-2","article-title":"Expression of SSX genes in human tumors","volume":"77","author":"Tureci","year":"1998","journal-title":"Int. J. Cancer J. Int. Cancer"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1158\/1078-0432.CCR-05-1902","article-title":"Expression of synovial sarcoma X (SSX) antigens in epithelial ovarian cancer and identification of SSX-4 epitopes recognized by CD4+ T cells","volume":"12","author":"Valmori","year":"2006","journal-title":"Clin. Cancer Res. Off. J. Am. Assoc. Cancer Res."},{"key":"ref_14","unstructured":"Grayson, K. (2023). Investigating Synovial Sarcoma, X Breakpoint Proteins in Ovarian Cancer, University of Hull."},{"key":"ref_15","unstructured":"Fearn, A., Doli\u0144ska, W., Grayson, K., Khan, G.N., Pulford, K., Wilkinson, H., and Guinn, B.A. SSX Family Members Play Different Roles in the Early Development of Ovarian Cancer, in preparation."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1038\/nrg3185","article-title":"Insights into the regulation of protein abundance from proteomic and transcriptomic analyses","volume":"13","author":"Vogel","year":"2012","journal-title":"Nat. Rev. Genet."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"106121","DOI":"10.1016\/j.biocel.2021.106121","article-title":"SSX2 promotes the formation of a novel type of intranuclear lamin bodies","volume":"142","author":"Traynor","year":"2022","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Alrubie, T.M., Alamri, A.M., Almutairi, B.O., Alrefaei, A.F., Arafah, M.M., Alanazi, M., Semlali, A., and Almutairi, M.H. (2023). Higher Expression Levels of SSX1 and SSX2 in Patients with Colon Cancer: Regulated In Vitro by the Inhibition of Methylation and Histone Deacetylation. Medicina, 59.","DOI":"10.3390\/medicina59050988"},{"key":"ref_19","first-page":"1986","article-title":"Cancer\/testis antigen SSX2 enhances invasiveness in MCF-7 cells by repressing ER\u03b1 signaling","volume":"40","author":"Chen","year":"2012","journal-title":"Int. J. Oncol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1038\/s41419-019-2068-1","article-title":"A functional genetic screen identifies the Mediator complex as essential for SSX2-induced senescence","volume":"10","author":"Bennedsen","year":"2019","journal-title":"Cell Death Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.molonc.2014.09.001","article-title":"Ectopic expression of cancer\/testis antigen SSX2 induces DNA damage and promotes genomic instability","volume":"9","author":"Greve","year":"2015","journal-title":"Mol. Oncol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"50997","DOI":"10.18632\/oncotarget.9802","article-title":"SSX2 regulates focal adhesion but does not drive the epithelial to mesenchymal transition in prostate cancer","volume":"7","author":"Bloom","year":"2016","journal-title":"Oncotarget"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2207","DOI":"10.1158\/0008-5472.CAN-04-1882","article-title":"Cancer\/testis antigen expression in human mesenchymal stem cells: Down-regulation of SSX impairs cell migration and matrix metalloproteinase 2 expression","volume":"65","author":"Cronwright","year":"2005","journal-title":"Cancer Res."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Bradbury, A., O\u2019Donnell, R., Drew, Y., Curtin, N., and Saha, S.S. (2020). Characterisation of Ovarian Cancer Cell Line NIH-OVCAR3 and Implications of Genomic, Transcriptomic, Proteomic and Functional DNA Damage Response Biomarkers for Therapeutic Targeting. Cancers, 12.","DOI":"10.3390\/cancers12071939"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.4049\/jimmunol.174.3.1709","article-title":"Naturally acquired MAGE-A10- and SSX-2-specific CD8+ T cell responses in patients with hepatocellular carcinoma","volume":"174","author":"Bricard","year":"2005","journal-title":"J. Immunol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1053\/j.gastro.2009.04.045","article-title":"Profile of tumor antigen-specific CD8 T cells in patients with hepatitis B virus-related hepatocellular carcinoma","volume":"137","author":"Gehring","year":"2009","journal-title":"Gastroenterology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1186\/1479-5876-11-52","article-title":"SSX2IP promotes metastasis and chemotherapeutic resistance of hepatocellular carcinoma","volume":"11","author":"Li","year":"2013","journal-title":"J. Transl. Med."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Lv, X., Kong, J., Chen, W.-D., and Wang, Y.-D. (2017). The Role of the Apelin\/APJ System in the Regulation of Liver Disease. Front. Pharmacol., 8.","DOI":"10.3389\/fphar.2017.00221"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"W216","DOI":"10.1093\/nar\/gkac194","article-title":"DAVID: A web server for functional enrichment analysis and functional annotation of gene lists (2021 update)","volume":"50","author":"Sherman","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"11433","DOI":"10.1093\/nar\/gku852","article-title":"SSX2 is a novel DNA-binding protein that antagonizes polycomb group body formation and gene repression","volume":"42","author":"Gjerstorff","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"6668","DOI":"10.1093\/nar\/gkz396","article-title":"Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins","volume":"47","author":"Traynor","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_32","unstructured":"Khan, G. (2016). Characterisation of the Expression of Tumour Antigens and Biomarkers in Myeloid Leukaemia and Ovarian Cancer, University of Bedfordshire."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1111\/tan.12340","article-title":"SSX2-4 expression in early-stage non-small cell lung cancer","volume":"83","author":"Greve","year":"2014","journal-title":"Tissue Antigens"},{"key":"ref_34","unstructured":"Frank, S.A. (2020). Dynamics of Cancer: Incidence, Inheritance, and Evolution, Princeton University Press."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1038\/s41580-019-0163-x","article-title":"Advances in protein structure prediction and design","volume":"20","author":"Kuhlman","year":"2019","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1038\/s41573-021-00387-y","article-title":"Identification of neoantigens for individualized therapeutic cancer vaccines","volume":"21","author":"Lang","year":"2022","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Day, C.A., Hinchcliffe, E.H., and Robinson, J.P. (2022). H3K27me3 in Diffuse Midline Glioma and Epithelial Ovarian Cancer: Opposing Epigenetic Changes Leading to the Same Poor Outcomes. Cells, 11.","DOI":"10.3390\/cells11213376"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1038\/nrc.2017.18","article-title":"The calcium-cancer signalling nexus","volume":"17","author":"Monteith","year":"2017","journal-title":"Nat. Rev. Cancer"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Wu, S., Lv, Z., Wang, Y., Sun, L., Jiang, Z., Xu, C., Zhao, J., Sun, X., Li, X., and Hu, L. (2013). Increased Expression of Pregnancy Up-Regulated Non-Ubiquitous Calmodulin Kinase Is Associated with Poor Prognosis in Clear Cell Renal Cell Carcinoma. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0059936"},{"key":"ref_40","first-page":"5571","article-title":"The caM kinase, Pnck, is spatially and temporally regulated during murine mammary gland development and may identify an epithelial cell subtype involved in breast cancer","volume":"60","author":"Gardner","year":"2000","journal-title":"Cancer Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/s13045-018-0574-8","article-title":"CAMKs support development of acute myeloid leukemia","volume":"11","author":"Kang","year":"2018","journal-title":"J. Hematol. Oncol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Tsuyoshi, H., Orisaka, M., Fujita, Y., Asare-Werehene, M., Tsang, B.K., and Yoshida, Y. (2020). Prognostic impact of Dynamin related protein 1 (Drp1) in epithelial ovarian cancer. BMC Cancer, 20.","DOI":"10.1186\/s12885-020-06965-4"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Bartha, \u00c1., and Gy\u0151rffy, B. (2021). TNMplot.com: A Web Tool for the Comparison of Gene Expression in Normal, Tumor and Metastatic Tissues. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22052622"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1016\/j.ygyno.2017.03.509","article-title":"NY-ESO-1 expression predicts an aggressive phenotype of ovarian cancer","volume":"145","author":"Szender","year":"2017","journal-title":"Gynecol. Oncol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Zhang, S., Zhou, X., Yu, H., and Yu, Y. (2010). Expression of tumor-specific antigen MAGE, GAGE and BAGE in ovarian cancer tissues and cell lines. BMC Cancer, 10.","DOI":"10.1186\/1471-2407-10-163"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"D\u2019Arcy, P., Maruwge, W., Wolahan, B., Ma, L., and Brodin, B. (2014). Oncogenic functions of the cancer-testis antigen SSX on the proliferation, survival, and signaling pathways of cancer cells. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0095136"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Steinbichler, T.B., Dudas, J., Ingruber, J., Glueckert, R., Sprung, S., Fleischer, F., Cidlinsky, N., Dejaco, D., Kofler, B., and Giotakis, A.I. (2020). Slug Is A Surrogate Marker of Epithelial to Mesenchymal Transition (EMT) in Head and Neck Cancer. J. Clin. Med., 9.","DOI":"10.3390\/jcm9072061"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"6797","DOI":"10.18632\/oncotarget.3180","article-title":"Snail and Slug collaborate on EMT and tumor metastasis through miR-101-mediated EZH2 axis in oral tongue squamous cell carcinoma","volume":"6","author":"Zheng","year":"2015","journal-title":"Oncotarget"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1177\/1933719116654989","article-title":"Slug Is Associated With Tumor Metastasis and Angiogenesis in Ovarian Cancer","volume":"24","author":"Gu","year":"2017","journal-title":"Reprod. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.bbrc.2009.04.051","article-title":"Molecular mechanisms controlling E-cadherin expression in breast cancer","volume":"384","author":"Baranwal","year":"2009","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1838","DOI":"10.1096\/fj.09-151639","article-title":"Vimentin induces changes in cell shape, motility, and adhesion during the epithelial to mesenchymal transition","volume":"24","author":"Mendez","year":"2010","journal-title":"FASEB J."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Rosso, M., Majem, B., Devis, L., Lapyckyj, L., Besso, M.J., Llaurad\u00f3, M., Abascal, M.F., Matos, M.L., Lanau, L., and Castellv\u00ed, J. (2017). E-cadherin: A determinant molecule associated with ovarian cancer progression, dissemination and aggressiveness. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0184439"},{"key":"ref_53","unstructured":"Blighe, K., Rana, S., and Lewis, M. (EnhancedVolcano: Publication-Ready Volcano Plots with Enhanced Colouring and Labelling [Internet], 2021). EnhancedVolcano: Publication-Ready Volcano Plots with Enhanced Colouring and Labelling [Internet], R package version 1.12. 0."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2648","DOI":"10.1111\/bph.70390","article-title":"TNMplot: An enhanced platform for pharmacological target identification through cross-stage and pan-cancer gene expression analysis","volume":"183","author":"Bartha","year":"2026","journal-title":"Br. J. Pharmacol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1889","DOI":"10.1007\/s11357-023-00742-4","article-title":"Discovery and ranking of the most robust prognostic biomarkers in serous ovarian cancer","volume":"45","author":"Gyorffy","year":"2023","journal-title":"Geroscience"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"440","DOI":"10.4161\/cam.36224","article-title":"An introduction to the wound healing assay using live-cell microscopy","volume":"8","author":"Jonkman","year":"2014","journal-title":"Cell Adhes. Migr."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Suarez-Arnedo, A., Figueroa, F.T., Clavijo, C., Arbel\u00e1ez, P., Cruz, J.C., and Mu\u00f1oz-Camargo, C. (2020). An image J plugin for the high throughput image analysis of in vitro scratch wound healing assays. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0232565"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1555","DOI":"10.1038\/sj.bjc.6605642","article-title":"Guidelines for the welfare and use of animals in cancer research","volume":"102","author":"Workman","year":"2010","journal-title":"Br. J. Cancer"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1136\/jcp.48.9.876","article-title":"A \u201cquickscore\u201d method for immunohistochemical semiquantitation: Validation for oestrogen receptor in breast carcinomas","volume":"48","author":"Detre","year":"1995","journal-title":"J. Clin. Pathol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/19\/8608\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:53:21Z","timestamp":1790614401000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/19\/8608"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,26]]},"references-count":61,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2026,10]]}},"alternative-id":["ijms27198608"],"URL":"https:\/\/doi.org\/10.3390\/ijms27198608","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,26]]}}}