{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:57:47Z","timestamp":1790830667186,"version":"4.1.0"},"reference-count":45,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:00:00Z","timestamp":1790380800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003627","name":"Rural Development Administration","doi-asserted-by":"publisher","award":["RS-2026-25518677"],"award-info":[{"award-number":["RS-2026-25518677"]}],"id":[{"id":"10.13039\/501100003627","id-type":"DOI","asserted-by":"publisher"}]},{"award":["RS-2026-25518677"],"award-info":[{"award-number":["RS-2026-25518677"]}],"id":[{"id":"https:\/\/ror.org\/03xs9yg50","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>The root bark of Ulmus davidiana var. japonica (Ulmaceae), commonly known as yugeunpi, has long been used in traditional Korean medicine to treat gastroenteric and inflammatory disorders, as well as to promote blood circulation and tissue regeneration. As part of our ongoing efforts to discover bioactive natural products from resources with pharmacological potential, a phytochemical investigation of the root bark of U. davidiana var. japonica was conducted, and the wound-healing potential of the isolated compounds was evaluated. This study led to the isolation of one new catechin glycoside derivative, (\u2013)-catechinulmusol (1) and two new neolignan derivatives, neolignulmusides A and B (2 and 3), along with ten known compounds (4\u201313), including lignans (4 and 5), flavonoids (6\u201310), and phenolic derivatives (11\u201313). The structures of the new compounds (1\u20133) were elucidated by extensive spectroscopic analyses, including 1D and 2D NMR, high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), and chemical derivatization. Results from in vitro assays revealed that compounds 1, 4, 6, and 13 significantly promoted the migration of human skin keratinocytes. These findings suggest that these active compounds may serve as promising candidates for skin wound-healing applications, including epidermal regeneration.<\/jats:p>","DOI":"10.3390\/ijms27198630","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:50:54Z","timestamp":1790614254000},"page":"8630","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Polyphenols from the Root Barks of Ulmus davidiana var. japonica and Their Wound Healing Potential"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3320-4662","authenticated-orcid":false,"given":"Jae Sik","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Integrative Biological Sciences and Industry, Sejong University, 209 Neungdong-ro, Gwangjin-gu, Seoul 05006, Republic of Korea"},{"name":"School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4551-4116","authenticated-orcid":false,"given":"Gakyung","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Integrative Biological Sciences and Industry, Sejong University, 209 Neungdong-ro, Gwangjin-gu, Seoul 05006, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sooyeon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Integrative Biological Sciences and Industry, Sejong University, 209 Neungdong-ro, Gwangjin-gu, Seoul 05006, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoon-Joo","family":"Ko","sequence":"additional","affiliation":[{"name":"Laboratory of Nuclear Magnetic Resonance, National Center for Inter-University Research Facilities (NCIRF), Seoul National University, Gwanak-gu, Seoul 08826, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min Cheol","family":"Kang","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Gachon University, Incheon 21936, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8044-5613","authenticated-orcid":false,"given":"Sun Yeou","family":"Kim","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Gachon University, Incheon 21936, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min Hye","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, College of Pharmacy and Research Institute for Drug Development, Pusan National University, Busan 46241, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5285-9138","authenticated-orcid":false,"given":"Ki Hyun","family":"Kim","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1038\/s41577-018-0084-5","article-title":"The immunological anatomy of the skin","volume":"19","author":"Kabashima","year":"2019","journal-title":"Nat. Rev. Immunol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1064","DOI":"10.1038\/ni.2992","article-title":"Perivascular leukocyte clusters are essential for efficient activation of effector T cells in the skin","volume":"15","author":"Natsuaki","year":"2014","journal-title":"Nat. Immunol."},{"key":"ref_3","first-page":"149","article-title":"NED416, a novel synthetic Sirt1 activator, promotes cutaneous wound healing via the MAPK\/Rho pathway","volume":"46","author":"Wahedi","year":"2020","journal-title":"Int. J. Mol. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1528","DOI":"10.1177\/147323000903700531","article-title":"The wound healing process: An overview of the cellular and molecular mechanisms","volume":"37","author":"Velnar","year":"2009","journal-title":"J. Int. Med. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"41","DOI":"10.3831\/KPI.2013.16.013","article-title":"Anti-allergic effect of Ulmus davidiana cortex on contact dermatitis induced by dinitrofluoro-benzene in mice","volume":"16","author":"Lyu","year":"2013","journal-title":"J. Pharmacopunct."},{"key":"ref_6","first-page":"217","article-title":"A study on efficacy of Ulmi cortex","volume":"21","author":"Hong","year":"1990","journal-title":"Kor. J. Pharmacogn."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1021\/np0004799","article-title":"Five Novel Neuroprotective Triterpene Esters of Ulmus davidiana var. japonica","volume":"64","author":"Lee","year":"2001","journal-title":"J. Nat. Prod."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1007\/BF02855558","article-title":"Catechin glycoside from Ulmus davidiana","volume":"12","author":"Son","year":"1989","journal-title":"Arch. Pharm. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/S0378-8741(98)00063-4","article-title":"Inhibition of nitric oxide synthesis by butanol fraction of the methanol extract of Ulmus davidiana in murine macrophages","volume":"62","author":"Jun","year":"1998","journal-title":"J. Ethnopharmacol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1016\/j.fct.2006.12.006","article-title":"Glycoprotein isolated from Ulmus davidiana Nakai regulates expression of iNOS and COX-2 in vivo and in vitro","volume":"45","author":"Lee","year":"2007","journal-title":"Food Chem. Toxicol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"321","DOI":"10.4062\/biomolther.2010.18.3.321","article-title":"Anti-inflammatory activity of constituents isolated from Ulmus davidiana var. japonica","volume":"18","author":"Zheng","year":"2010","journal-title":"Biomol. Ther."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1443","DOI":"10.1007\/s12272-011-0905-6","article-title":"Protective constituents against sepsis in mice from the root barks of Ulmus davidiana var. japonica","volume":"34","author":"Zheng","year":"2011","journal-title":"Arch. Pharm. Res."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Kang, M.C., Yumnam, S., Park, W.S., So, H.M., Kim, K.H., Shin, M.C., Ahn, M.J., and Kim, S.Y. (2020). Ulmus parvifolia accelerates skin wound healing by regulating the expression of MMPs and TGF-\u03b2. J. Clin. Med., 9.","DOI":"10.20944\/preprints201911.0171.v1"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Lee, S., Yu, J.S., Phung, H.M., Lee, J.G., Kim, K.H., and Kang, K.S. (2020). Potential anti-skin aging effect of (-)-catechin isolated from the root bark of Ulmus davidiana var. japonica in tumor necrosis factor-\u03b1-stimulated normal human dermal fibroblasts. Antioxidants, 9.","DOI":"10.3390\/antiox9100981"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1007\/s12272-024-01486-1","article-title":"New autophagy-modulating lanostane-type triterpenoids from a hallucinogenic poisonous mushroom Gymnopilus orientispectabilis","volume":"47","author":"Lee","year":"2024","journal-title":"Arch. Pharm. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1007\/s12272-025-01553-1","article-title":"Activation of cryptic biosynthetic pathways in Saccharopolyspora spinosa through deletion of the spinosyn gene cluster: Induction of cryptic and bioactive natural products","volume":"48","author":"Bridget","year":"2025","journal-title":"Arch. Pharm. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"262","DOI":"10.20307\/nps.2024.30.4.262","article-title":"Aster glehni ethanol extract inhibits inflammatory responses regulating skin barrier molecules in human keratinocytes","volume":"30","author":"Gil","year":"2024","journal-title":"Nat. Prod. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"135","DOI":"10.20307\/nps.2024.30.2.135","article-title":"Phenolic compounds isolated from Juncus decipiens and their effects on osteoblast differentiation in the mouse mesenchymal stem cell line C3H10T1\/2","volume":"30","author":"Cho","year":"2024","journal-title":"Nat. Prod. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e07944","DOI":"10.1002\/advs.202507944","article-title":"Alternative models for anticancer drug discovery from natural products using binary tumor-microenvironment-on-a-chip","volume":"12","author":"Kim","year":"2025","journal-title":"Adv. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1897","DOI":"10.1016\/j.chom.2024.09.014","article-title":"Vaginal lactobacilli produce anti-inflammatory \u03b2-carboline compounds","volume":"32","author":"Glick","year":"2024","journal-title":"Cell Host Microbe"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Hong, E.Y., Kim, T.Y., Hong, G.U., Kang, H., Lee, J.Y., Park, J.Y., Kim, S.C., Kim, Y.H., Chung, M.H., and Kwon, Y.I. (2019). Inhibitory effects of roseoside and icariside E4 isolated from a natural product mixture (No-ap) on the expression of angiotensin II receptor 1 and oxidative stress in angiotensin II-stimulated H9C2 cells. Molecules, 24.","DOI":"10.3390\/molecules24030414"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.bmcl.2017.11.045","article-title":"(\u2212)-9\u2032-O-(\u03b1-l-Rhamnopyranosyl) lyoniresinol from Lespedeza cuneata suppresses ovarian cancer cell proliferation through induction of apoptosis","volume":"28","author":"Baek","year":"2018","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"136","DOI":"10.7324\/JAPS.2017.71120","article-title":"Steroids and isoquercetin from Lygodium microphyllum","volume":"7","author":"Kuncoro","year":"2017","journal-title":"J. Appl. Pharm. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1016\/S0367-326X(99)00084-2","article-title":"Constituents from the roots of Bowdichia virgilioides","volume":"70","author":"Velozo","year":"1999","journal-title":"Fitoterapia"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.1016\/0031-9422(95)00383-I","article-title":"Syringetin 3-O-(6\u2033-acetyl)-\u03b2-glucopyranoside and other flavonols from needles of Norway spruce, Picea abies","volume":"40","author":"Slimestad","year":"1995","journal-title":"Phytochemistry"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1071\/CH9650531","article-title":"Nuclear magnetic resonance spectra and structures of some C-glycosyl flavonoids","volume":"18","author":"Hillis","year":"1965","journal-title":"Aust. J. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2177","DOI":"10.1016\/j.phytochem.2005.02.002","article-title":"Circular dichroism, a powerful tool for the assessment of absolute configuration of flavonoids","volume":"66","author":"Slade","year":"2005","journal-title":"Phytochemistry"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"103145","DOI":"10.1016\/j.bioorg.2019.103145","article-title":"Chemical constituents of the root bark of Ulmus davidiana var. japonica and their potential biological activities","volume":"91","author":"So","year":"2019","journal-title":"Bioorg. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/j.jff.2015.12.009","article-title":"Antioxidants, quinone reductase inducers and acetylcholinesterase inhibitors from Spondias tuberosa fruits","volume":"21","author":"Zeraik","year":"2016","journal-title":"J. Funct. Foods"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1007\/s12272-011-0808-6","article-title":"Phenolic constituents of Acorus gramineus","volume":"34","author":"Park","year":"2011","journal-title":"Arch. Pharm. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1002\/(SICI)1097-458X(199610)34:10<755::AID-OMR950>3.0.CO;2-U","article-title":"1H and 13C NMR shifts for aldopyranose and aldofuranose monosaccharides: Conformational analysis and solvent dependence","volume":"34","author":"Hobley","year":"1996","journal-title":"Magn. Reson. Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1858","DOI":"10.1021\/acssynbio.9b00171","article-title":"Diversity of O-glycosyltransferases contributes to the biosynthesis of flavonoid and triterpenoid glycosides in Glycyrrhiza uralensis","volume":"8","author":"Chen","year":"2019","journal-title":"ACS Synth. Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1111\/j.2042-7158.2010.01162.x","article-title":"Anti-inflammatory potential of Phaseolus calcaratus Roxburgh, a oriental medicine, on LPS-stimulated RAW 264.7 macrophages","volume":"63","author":"Fang","year":"2011","journal-title":"J. Pharm. Pharmacol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1516","DOI":"10.1007\/s12272-013-0143-1","article-title":"Chemical constituents from Inula wissmanniana and their anti-inflammatory activities","volume":"36","author":"Wang","year":"2013","journal-title":"Arch. Pharm. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.1016\/S0031-9422(97)00198-2","article-title":"Proanthocyanidins from Lotus pedunculatus","volume":"45","author":"Foo","year":"1997","journal-title":"Phytochemistry"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"5459","DOI":"10.1016\/S0040-4020(01)91712-0","article-title":"Circular dichroism spectra of flavanols","volume":"27","author":"Korver","year":"1971","journal-title":"Tetrahedron"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1248\/cpb.55.899","article-title":"Facile discrimination of aldose enantiomers by reversed-phase HPLC","volume":"55","author":"Tanaka","year":"2007","journal-title":"Chem. Pharm. Bull."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1174","DOI":"10.1021\/acs.jnatprod.5b00090","article-title":"Lignan glycosides from the twigs of Chaenomeles sinensis and their biological activities","volume":"78","author":"Kim","year":"2015","journal-title":"J. Nat. Prod."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"12182","DOI":"10.1016\/j.tet.2006.10.018","article-title":"Synthesis of dihydrodehydrodiconiferyl alcohol and derivatives through intramolecular C\u2013H insertion","volume":"62","author":"Rosales","year":"2006","journal-title":"Tetrahedron"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2467","DOI":"10.1002\/hlca.201000116","article-title":"Neolignans and caffeoyl derivatives from Selaginella moellendorffii","volume":"93","author":"Wang","year":"2010","journal-title":"Helv. Chim. Acta"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Nishida, Y., Aono, R., Dohi, H., Ding, W.X., and Uzawa, H. (2023). 1H-NMR Karplus analysis of molecular conformations of glycerol under different solvent conditions: A consistent rotational isomerism in the backbone governed by glycerol\/water interactions. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24032766"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Kim, T.-Y., Lee, B.S., Jo, B.-G., Heo, S.P., Keem, M.-J., Kwon, T.-H., Kim, S.-N., Kim, K.H., and Yang, M.H. (2023). 3\u2034-O-Foliamenthoyl-Rutin, a New Flavonoid Glycoside from the Roots of Nymphoides peltata. Plants, 12.","DOI":"10.3390\/plants12244083"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/s41038-016-0032-1","article-title":"Evaluation of the wound healing potential of isoquercetin-based cream on scald burn injury in rats","volume":"4","author":"Bhatia","year":"2016","journal-title":"Burns Trauma"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1021\/acs.jnatprod.5b01042","article-title":"Furofuran lignan glucosides with estrogen-inhibitory properties from the Bangladeshi medicinal plant Terminalia citrina","volume":"79","author":"Muhit","year":"2016","journal-title":"J. Nat. Prod."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"3065","DOI":"10.1021\/acs.jnatprod.6b00720","article-title":"Antioxidant flavonols and phenolic compounds from Atraphaxis frutescens and their inhibitory activities against insect phenoloxidase and mushroom tyrosinase","volume":"79","author":"Odonbayar","year":"2016","journal-title":"J. Nat. Prod."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/19\/8630\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:21:01Z","timestamp":1790828461000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/19\/8630"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,26]]},"references-count":45,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2026,10]]}},"alternative-id":["ijms27198630"],"URL":"https:\/\/doi.org\/10.3390\/ijms27198630","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,26]]}}}