{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:00:04Z","timestamp":1790830804742,"version":"4.1.0"},"reference-count":63,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:00:00Z","timestamp":1790380800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2022YFD1200404"],"award-info":[{"award-number":["2022YFD1200404"]}]},{"name":"National Natural Science Foundation of China","award":["32501906"],"award-info":[{"award-number":["32501906"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"crossref"}]},{"award":["32501906"],"award-info":[{"award-number":["32501906"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Yuelushan Laboratory Breeding Program","award":["50225100802"],"award-info":[{"award-number":["50225100802"]}]},{"name":"Science and Technology Innovation Leading Talent Project of Hunan Province","award":["2023RC1063"],"award-info":[{"award-number":["2023RC1063"]}]},{"name":"Hunan Provincial Natural Science Foundation","award":["2024JJ6233"],"award-info":[{"award-number":["2024JJ6233"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>The UMAMIT (Usually Multiple Acids Move In and Out Transporters) family comprises plant-specific amino acid transporters that play key roles in nitrogen uptake, transport, and storage. As a major oil crop in the Brassicaceae family, efficient amino acid transport in Brassica napus seeds is closely linked to seed yield and quality. Despite extensive studies in other species, the UMAMIT gene family has not been systematically characterized in B. napus. In this study, we identified 172 BnaUMAMIT genes, all of which harbor the EamA\/WAT1-related domain and are predicted to localize to the plasma membrane. Both tandem and whole-genome\/segmental duplications contributed to the expansion of this gene family. Phylogenetic analysis clustered BnaUMAMIT proteins into eight clades, revealing extensive collinearity with homologs in Arabidopsis thaliana, Brassica rapa, and Brassica oleracea. Gene structure analysis revealed conserved exon\u2013intron organization within subgroups, whereas promoter analysis revealed diverse cis-acting elements associated with stress responses, hormone signaling, and developmental regulation. Transcriptome profiling revealed that 6 BnaUMAMIT genes were more highly expressed in low-oil-content seeds than in high-oil-content seeds at 25, 35, and 45 days after pollination in the two different environments, suggesting a possible association with seed oil content. Weighted gene co-expression network analysis (WGCNA) further demonstrated a significant correlative relationship between BnaUMAMIT transcripts and seed oil accumulation; meanwhile, co-expression network analysis revealed that BnaUMAMITs are correlated with the expression of lipid synthesis-related transcription factors, such as BnaTT8, BnaSTK, BnaGL2, BnaLEC1, and BnaLEC2, suggesting a potential regulatory association involved in seed oil accumulation. However, these results remain correlative and require further functional validation. Taken together, the results of this study provide a comprehensive understanding of the evolution, structure, and expression of the BnaUMAMIT gene family, laying a foundation for future functional studies and potential improvements in seed oil content in B. napus.<\/jats:p>","DOI":"10.3390\/ijms27198635","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:50:54Z","timestamp":1790614254000},"page":"8635","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome-Wide Identification and Characterization of the UMAMIT Gene Family in Brassica napus: Evolution, Expression, and Potential Association with Oil Content"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-4117-5791","authenticated-orcid":false,"given":"Dan","family":"He","sequence":"first","affiliation":[{"name":"College of Agronomy, Hunan Agricultural University, Changsha 410128, China"},{"name":"Yuelushan Laboratory, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Agronomy, Hunan Agricultural University, Changsha 410128, China"},{"name":"Yuelushan Laboratory, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tongxiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Agronomy, Hunan Agricultural University, Changsha 410128, China"},{"name":"Yuelushan Laboratory, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8602-5166","authenticated-orcid":false,"given":"Xin","family":"He","sequence":"additional","affiliation":[{"name":"College of Agronomy, Hunan Agricultural University, Changsha 410128, China"},{"name":"Yuelushan Laboratory, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Qiu","sequence":"additional","affiliation":[{"name":"College of Agronomy, Hunan Agricultural University, Changsha 410128, China"},{"name":"Yuelushan Laboratory, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongsong","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Agronomy, Hunan Agricultural University, Changsha 410128, China"},{"name":"Yuelushan Laboratory, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lunwen","family":"Qian","sequence":"additional","affiliation":[{"name":"College of Agronomy, Hunan Agricultural University, Changsha 410128, China"},{"name":"Yuelushan Laboratory, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Liu, S., Snowdon, R., and Chalhoub, B. (2018). Academic and economic importance of Brassica napus rapeseed. The Brassica napus Genome, Springer International Publishing.","DOI":"10.1007\/978-3-319-43694-4"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Gosal, S.S., and Wani, S.H. (2022). Breeding major oilseed crops: Prospects and future research needs. Accelerated Plant Breeding, Volume 4: Oil Crops, Springer International Publishing.","DOI":"10.1007\/978-3-030-81107-5"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1016\/j.molp.2020.12.003","article-title":"Genome- and transcriptome-wide association studies provide insights into the genetic basis of natural variation of seed oil content in Brassica napus","volume":"14","author":"Tang","year":"2021","journal-title":"Mol. Plant"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"koaf062","DOI":"10.1093\/plcell\/koaf062","article-title":"Genetic and transcriptome analyses of the effect of genotype-by-environment interactions on Brassica napus seed oil content","volume":"37","author":"Han","year":"2025","journal-title":"Plant Cell"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2799","DOI":"10.1111\/jipb.70017","article-title":"Genetic optimization of the source, sink and flow for increasing seed oil content in rapeseed","volume":"67","author":"Shen","year":"2025","journal-title":"J. Integr. Plant Biol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1016\/j.plaphy.2007.09.009","article-title":"Carnitine is associated with fatty acid metabolism in plants","volume":"45","author":"Bourdin","year":"2007","journal-title":"Plant Physiol. Biochem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1016\/j.biortech.2015.08.100","article-title":"Effects of various amino acids as organic nitrogen sources on the growth and biochemical composition of Chlorella pyrenoidosa","volume":"197","author":"Zhang","year":"2015","journal-title":"Bioresour. Technol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1016\/j.plantsci.2018.06.020","article-title":"Physiology of L-carnitine in plants in light of the knowledge in animals and microorganisms","volume":"274","author":"Jacques","year":"2018","journal-title":"Plant Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3405","DOI":"10.1007\/s00253-022-11931-0","article-title":"The relationship between amino acid and lipid metabolism in oleaginous eukaryotic microorganism","volume":"106","author":"Cai","year":"2022","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1071\/AR01203","article-title":"Influence of genotype and environment on oil and protein concentrations of canola (Brassica napus L.) grown across southern Australia","volume":"54","author":"Si","year":"2003","journal-title":"Aust. J. Agric. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.1105\/tpc.113.111740","article-title":"Seed architecture shapes embryo metabolism in oilseed rape","volume":"25","author":"Borisjuk","year":"2013","journal-title":"Plant Cell"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"46295","DOI":"10.1038\/srep46295","article-title":"Genetic dissection of seed oil and protein content and identification of networks associated with oil content in Brassica napus","volume":"7","author":"Chao","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3603","DOI":"10.1105\/tpc.110.073833","article-title":"Altered xylem-phloem transfer of amino acids affects metabolism and leads to increased seed yield and oil content in Arabidopsis","volume":"22","author":"Zhang","year":"2010","journal-title":"Plant Cell"},{"key":"ref_14","first-page":"848","article-title":"Histidine regulates seed oil deposition through abscisic acid biosynthesis and \u03b2-oxidation1","volume":"172","author":"Ma","year":"2016","journal-title":"Plant Physiol."},{"key":"ref_15","first-page":"577","article-title":"Transgenic canola and soybean seeds with increased lysine","volume":"13","author":"Falco","year":"1995","journal-title":"Bio\/Technology"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/S0005-2736(00)00144-9","article-title":"Amino acid transporters in plants","volume":"1465","author":"Chang","year":"2000","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1038\/nature11909","article-title":"Using membrane transporters to improve crops for sustainable food production","volume":"497","author":"Schroeder","year":"2013","journal-title":"Nature"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.semcdb.2017.07.010","article-title":"Update on amino acid transporter functions and on possible amino acid sensing mechanisms in plants","volume":"74","author":"Dinkeloo","year":"2018","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1016\/j.cj.2021.04.002","article-title":"Function, transport, and regulation of amino acids: What is missing in rice?","volume":"9","author":"Guo","year":"2021","journal-title":"Crop J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1038\/s41586-023-05969-x","article-title":"Export of defensive glucosinolates is key for their accumulation in seeds","volume":"617","author":"Xu","year":"2023","journal-title":"Nature"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Yang, G., Wei, Q., Huang, H., and Xia, J. (2020). Amino acid transporters in plant cells: A brief review. Plants, 9.","DOI":"10.3390\/plants9080967"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Yao, X., Nie, J., Bai, R., and Sui, X. (2020). Amino acid transporters in plants: Identification and function. Plants, 9.","DOI":"10.3390\/plants9080972"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1111\/j.1469-8137.2008.02589.x","article-title":"Root uptake of cationic amino acids by Arabidopsis depends on functional expression of amino acid permease 5","volume":"180","author":"Svennerstam","year":"2008","journal-title":"New Phytol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1111\/j.1469-8137.2011.03699.x","article-title":"Transporters in Arabidopsis roots mediating uptake of amino acids at naturally occurring concentrations","volume":"191","author":"Svennerstam","year":"2011","journal-title":"New Phytol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3126","DOI":"10.1016\/j.cub.2015.10.038","article-title":"Amino acid export in developing Arabidopsis seeds depends on UmamiT facilitators","volume":"25","author":"Fastner","year":"2015","journal-title":"Curr. Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5221","DOI":"10.1093\/jxb\/ery302","article-title":"Arabidopsis UMAMIT24 and UMAMIT25 are amino acid exporters involved in seed loading","volume":"69","author":"Besnard","year":"2018","journal-title":"J. Exp. Bot."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s10265-024-01596-3","article-title":"New insights into the evolution and function of the UMAMIT (usually multiple acids move in and out transporter) gene family","volume":"138","author":"Cao","year":"2025","journal-title":"J. Plant Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1093\/pcp\/pct198","article-title":"Emerging functions of nodulin-like proteins in non-nodulating plant species","volume":"55","author":"Szurek","year":"2014","journal-title":"Plant Cell Physiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1643","DOI":"10.1104\/pp.111.192583","article-title":"SIARed1 from Arabidopsis acts as a bidirectional amino acid transporter that is crucial for the amino acid homeostasis of siliques","volume":"158","author":"Ladwig","year":"2012","journal-title":"Plant Physiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2625","DOI":"10.1038\/ncomms3625","article-title":"Arabidopsis WAT1 is a vacuolar auxin transport facilitator required for auxin homoeostasis","volume":"4","author":"Ranocha","year":"2013","journal-title":"Nat. Commun."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"6400","DOI":"10.1093\/jxb\/erab288","article-title":"Detailed characterization of the UMAMIT proteins provides insight into their evolution, amino acid transport properties, and role in the plant","volume":"72","author":"Zhao","year":"2021","journal-title":"J. Exp. Bot."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1093\/plcell\/koad310","article-title":"UMAMIT44 is a key player in glutamate export from Arabidopsis chloroplasts","volume":"36","author":"The","year":"2024","journal-title":"Plant Cell"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Meyer, L., Crocoll, C., Halkier, B.A., Mirza, O.A., and Xu, D. (2023). Identification of key amino acid residues in AtUMAMIT29 for transport of glucosinolates. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1219783"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1038\/s41598-021-04284-7","article-title":"Tissue specific expression of UMAMIT amino acid transporters in wheat","volume":"12","author":"Fang","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1111\/j.1365-313X.2007.03045.x","article-title":"AAP1 transports uncharged amino acids into roots of Arabidopsis","volume":"50","author":"Lee","year":"2007","journal-title":"Plant J."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1111\/j.1365-313X.2009.03890.x","article-title":"AAP1 regulates import of amino acids into developing Arabidopsis embryos","volume":"59","author":"Sanders","year":"2009","journal-title":"Plant J."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1302","DOI":"10.4161\/psb.5.10.13103","article-title":"Light-regulated compensation of wat1 (walls are thin1) growth and secondary cell wall phenotypes is auxin-independent","volume":"5","author":"Ranocha","year":"2010","journal-title":"Plant Signal. Behav."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1111\/j.1365-313X.2010.04256.x","article-title":"Walls are thin 1 (WAT1), an Arabidopsis homolog of Medicago truncatula NODULIN21, is a tonoplast-localized protein required for secondary wall formation in fibers","volume":"63","author":"Ranocha","year":"2010","journal-title":"Plant J."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1016\/j.jplph.2013.11.012","article-title":"Characterization of the putative amino acid transporter genes AtCAT2, 3 & 4: The tonoplast localized AtCAT2 regulates soluble leaf amino acids","volume":"171","author":"Yang","year":"2014","journal-title":"J. Plant Physiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"950","DOI":"10.1126\/science.1253435","article-title":"Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome","volume":"345","author":"Chalhoub","year":"2014","journal-title":"Science"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1534\/genetics.105.042093","article-title":"Segmental structure of the Brassica napus genome based on comparative analysis with Arabidopsis thaliana","volume":"171","author":"Parkin","year":"2005","journal-title":"Genetics"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1073\/pnas.1109047109","article-title":"Divergence of duplicate genes in exon\u2013intron structure","volume":"109","author":"Xu","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Ilg\u00fc, H., Jeckelmann, J.M., Kalbermatter, D., Ucurum, Z., Lemmin, T., and Fotiadis, D. (2021). High-resolution structure of the amino acid transporter AdiC reveals insights into the role of water molecules and networks in oligomerization and substrate binding. BMC Biol., 19.","DOI":"10.1186\/s12915-021-01102-4"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Besnard, J., Sonawala, U., Maharjan, B., Collakova, E., Finlayson, S.A., Pilot, G., McDowell, J., and Okumoto, S. (2021). Increased expression of UMAMIT amino acid transporters results in activation of salicylic acid-dependent stress response. Front. Plant Sci., 11.","DOI":"10.3389\/fpls.2020.606386"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"3846","DOI":"10.1093\/jxb\/erab103","article-title":"Cis-regulation of the amino acid transporter genes ZmAAP2 and ZmLHT1 by ZmPHR1 transcription factors in maize ear under phosphate limitation","volume":"72","author":"Wang","year":"2021","journal-title":"J. Exp. Bot."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"6385","DOI":"10.1093\/jxb\/erw412","article-title":"UMAMIT14 is an amino acid exporter involved in phloem unloading in Arabidopsis roots","volume":"67","author":"Besnard","year":"2016","journal-title":"J. Exp. Bot."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1111\/j.1439-0523.2005.01131.x","article-title":"Recurrent selection for increased protein content in yellow mustard (Sinapis alba L.)","volume":"124","author":"Raney","year":"2005","journal-title":"Plant Breed."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1016\/j.biotechadv.2009.07.001","article-title":"Increasing the flow of carbon into seed oil","volume":"27","author":"Weselake","year":"2009","journal-title":"Biotechnol. Adv."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Zhang, Z., Dunwell, J.M., and Zhang, Y.M. (2018). An integrated omics analysis reveals molecular mechanisms that are associated with differences in seed oil content between Glycine max and Brassica napus. BMC Plant Biol., 18.","DOI":"10.1186\/s12870-018-1542-8"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1111\/j.1365-313X.2007.03092.x","article-title":"WRINKLED1 specifies the regulatory action of LEAFY COTYLEDON2 towards fatty acid metabolism during seed maturation in Arabidopsis","volume":"50","author":"Baud","year":"2007","journal-title":"Plant J."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.plaphy.2015.12.022","article-title":"Modification of oil and glucosinolate content in canola seeds with altered expression of Brassica napus LEAFY COTYLEDON1","volume":"100","author":"Elahi","year":"2016","journal-title":"Plant Physiol. Biochem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"e2417264122","DOI":"10.1073\/pnas.2417264122","article-title":"Natural variations in TT8 and its neighboring STK confer yellow seed with elevated oil content in Brassica juncea","volume":"122","author":"Qian","year":"2025","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1007\/s00122-025-05146-w","article-title":"Joint analysis of GWAS and TILLING reveals the negative role of GLABRA2 in controlling seed oil content in Brassica napus","volume":"139","author":"Bai","year":"2026","journal-title":"Theor. Appl. Genet."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"iyae027","DOI":"10.1093\/genetics\/iyae027","article-title":"TAIR: The Arabidopsis information resource in 2024","volume":"227","author":"Reiser","year":"2024","journal-title":"Genetics"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"D1432","DOI":"10.1093\/nar\/gkab1057","article-title":"BRAD V3.0: An upgraded Brassicaceae database","volume":"50","author":"Chen","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"D444","DOI":"10.1093\/nar\/gkae1082","article-title":"InterPro: The protein sequence classification resource in 2025","volume":"53","author":"Blum","year":"2025","journal-title":"Nucleic Acids Res."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"D257","DOI":"10.1093\/nar\/gku949","article-title":"SMART: Recent updates, new developments and status in 2015","volume":"43","author":"Letunic","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"W521","DOI":"10.1093\/nar\/gkae241","article-title":"The EMBL-EBI job dispatcher sequence analysis tools framework in 2024","volume":"52","author":"Madeira","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.1016\/j.molp.2023.09.010","article-title":"TBtools-II: A \u201cone for all, all for one\u201d bioinformatics platform for biological big-data mining","volume":"16","author":"Chen","year":"2023","journal-title":"Mol. Plant"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1093\/nar\/30.1.325","article-title":"PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences","volume":"30","author":"Lescot","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Chao, H., Li, T., Luo, C., Huang, H., Ruan, Y., Li, X., Niu, Y., Fan, Y., Sun, W., and Zhang, K. (2020). BrassicaEDB: A gene expression database for Brassica crops. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21165831"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Jia, Y., Yao, M., He, X., Xiong, X., Guan, M., Liu, Z., Guan, C., and Qian, L. (2022). Transcriptome and regional association analyses reveal the effects of oleosin genes on the accumulation of oil content in Brassica napus. Plants, 11.","DOI":"10.3390\/plants11223140"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1101\/gr.1239303","article-title":"Cytoscape: A software environment for integrated models of biomolecular interaction networks","volume":"13","author":"Shannon","year":"2003","journal-title":"Genome Res."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/19\/8635\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:22:38Z","timestamp":1790828558000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/19\/8635"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,26]]},"references-count":63,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2026,10]]}},"alternative-id":["ijms27198635"],"URL":"https:\/\/doi.org\/10.3390\/ijms27198635","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,26]]}}}