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J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Bang sensitivity (BS) is a well-characterized seizure-and-paralysis phenotype in Drosophila melanogaster, but its genetic basis remains unexamined in the phylogenetically distant sister species Drosophila funebris. Here, we identify a naturally occurring BS strain in D. funebris from a wild population near Lake Valday, characterized by acute mechanical shock-induced seizures and paralysis, and examine its molecular-genetic and lipidomic basis. Comparative transcriptomics identified 241 differentially expressed genes (FDR-adjusted p &lt; 0.05), with \u201cfatty acid metabolic process\u201d as the most enriched GO term. Notably, within this pathway, the peroxisomal acyl-CoA oxidase gene Acox57D-d showed the most significant downregulation (log2FC = \u22124.21, padj = 4.1 \u00d7 10\u2212110), validated by head-specific RT-qPCR. Untargeted lipidomics of BS heads (LC-Q-TOF-MS) revealed 145 altered lipid species, dominated by oxygenated triacylglycerol accumulation, cardiolipin\/phosphatidylethanolamine depletion, and a divergent shift\u2014depletion of DG 29:0 and accumulation of DG 47:4, reflecting impaired peroxisomal \u03b2-oxidation and potential mitochondrial and Na+\/K+-ATPase dysfunction. These findings nominate Acox57D-d as a leading candidate gene associated with the phenotype and molecular signature of the studied BS line and establish this natural BS strain as a potentially useful model for investigating the relationship between peroxisomal lipid metabolism, neuronal excitability, and insect thanatosis.<\/jats:p>","DOI":"10.3390\/ijms27198845","type":"journal-article","created":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:31:31Z","timestamp":1791203491000},"page":"8845","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The First Report of a Bang-Sensitive Strain in Drosophila funebris: Molecular-Genetic and Lipidomic Characterization"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5699-9878","authenticated-orcid":false,"given":"Karina A.","family":"Kosherova","sequence":"first","affiliation":[{"name":"Vavilov Institute of General Genetics Russian Academy of Sciences, Gubkin St. 3, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6659-9851","authenticated-orcid":false,"given":"Dmitrii A.","family":"Abashkin","sequence":"additional","affiliation":[{"name":"Mental Health Research Center, Clinical Genetics Laboratory, Kashirskoe Sh. 34, 115522 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"}]},{"given":"Natalya B.","family":"Zakharzhevskaya","sequence":"additional","affiliation":[{"name":"Department of Postgenomics, Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, 119435 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0788-6938","authenticated-orcid":false,"given":"Victoriia D.","family":"Kazakova","sequence":"additional","affiliation":[{"name":"Department of Postgenomics, Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, 119435 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Elizaveta A.","family":"Vorobyeva","sequence":"additional","affiliation":[{"name":"Department of Postgenomics, Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, 119435 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1473-4470","authenticated-orcid":false,"given":"Alexander V.","family":"Symonenko","sequence":"additional","affiliation":[{"name":"National Research Centre \u201cKurchatov Institute\u201d, 2 Kurchatov Sq., 123182 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7380-0776","authenticated-orcid":false,"given":"Dmitry V.","family":"Mukha","sequence":"additional","affiliation":[{"name":"Vavilov Institute of General Genetics Russian Academy of Sciences, Gubkin St. 3, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,10,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1093\/genetics\/100.4.597","article-title":"Indirect suppression involving behavioral mutants with altered nerve excitability in drosophila 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