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J. Mol. Sci.","IJMS"],"abstract":"<jats:p>All four dengue virus serotypes (DENV-1 to DENV-4) can cause severe and potentially fatal disease. Understanding the interplay between host immune responses and viral genetic diversity is particularly crucial in hyperendemic regions, where serotype shifts frequently occur during outbreaks. In this cross-sectional, surveillance-based study, we analyzed 350 patients with confirmed secondary dengue infection in Lao PDR, with samples collected between 2022 and 2023. Of these, 80 were successfully serotyped by RT-PCR and included in the serotype-specific analyses. Viral genotyping was performed using envelope (E) gene sequencing, and neutralizing antibody (nAb) titers and antibody-dependent enhancement (ADE) activities against all four DENV serotypes were assessed using single-round infectious particles in both Fc\u03b3RIIA-negative and Fc\u03b3RIIA-positive BHK-21 cells. The nAb titers were highest against DENV-2, followed by DENV-1, DENV-4, and DENV-3. Nevertheless, DENV-2 exhibited the highest ADE activity, suggesting that high nAb titers may not necessarily prevent ADE. We also documented the co-circulation of DENV-2 Cosmopolitan and Asian I genotypes. Our findings highlight the complex interplay between neutralizing and enhancing antibody responses during secondary infection, particularly in the context of DENV-2 infection. Our results emphasize the need to understand the dynamics of DENV transmission in hyperendemic settings and may inform future vaccine development and evaluation strategies.<\/jats:p>","DOI":"10.3390\/ijms27198869","type":"journal-article","created":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:31:31Z","timestamp":1791203491000},"page":"8869","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["DENV-2 Genetic Diversity and Variation in Antibody-Dependent Enhancement in Lao PDR"],"prefix":"10.3390","volume":"27","author":[{"given":"Dalouny","family":"Xayavong","sequence":"first","affiliation":[{"name":"Department of Virology, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan"},{"name":"Graduate School of Biomedical Sciences, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan"},{"name":"Program for Nurturing Global Leaders in Tropical and Emerging Communicable Diseases, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan"},{"name":"National Center for Laboratory and Epidemiology, Ministry of Health, Vientiane 01002, Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virasack","family":"Somoulay","sequence":"additional","affiliation":[{"name":"National Center for Laboratory and Epidemiology, Ministry of Health, Vientiane 01002, Laos"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Bouaphanh","family":"Khamphaphongphane","sequence":"additional","affiliation":[{"name":"National Center for Laboratory and Epidemiology, Ministry of Health, Vientiane 01002, Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phonepadith","family":"Xangxayarath","sequence":"additional","affiliation":[{"name":"Department of Communicable Diseases Control, Ministry of Health, Vientiane 01002, 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Michie, A., Sasmono, R.T., and Imrie, A. (2020). Dengue: A Minireview. Viruses, 12.","DOI":"10.3390\/v12080829"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.meegid.2018.04.032","article-title":"Understanding dengue virus evolution to support epidemic surveillance and counter-measure development","volume":"62","author":"Pollett","year":"2018","journal-title":"Infect. Genet. Evol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2674","DOI":"10.4161\/hv.29563","article-title":"The relevance of dengue virus genotypes surveillance at country level before vaccine approval","volume":"10","author":"Laiton","year":"2014","journal-title":"Hum. Vaccines Immunother."},{"key":"ref_6","unstructured":"World Health Organization\/Department of Control of Neglected Tropical Diseases and TDR (2009). Dengue: Guidelines for Diagnosis, Treatment, Prevention and Control, World Health Organization."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1016\/j.jmii.2024.07.004","article-title":"Risk of severe dengue during secondary infection: A population-based cohort study in Taiwan","volume":"57","author":"Shih","year":"2024","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1186\/s40249-021-00908-2","article-title":"Clinical predictors of severe dengue: A systematic review and meta-analysis","volume":"10","author":"Tsheten","year":"2021","journal-title":"Infect. Dis. Poverty"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/s40794-024-00231-2","article-title":"The role of antibody-dependent enhancement in dengue vaccination","volume":"10","author":"Mersha","year":"2024","journal-title":"Trop. Dis. Travel Med. Vaccines"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1126\/science.aan6836","article-title":"Antibody-dependent enhancement of severe dengue disease in humans","volume":"358","author":"Katzelnick","year":"2017","journal-title":"Science"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Patro, A.R.K., Mohanty, S., Prusty, B.K., Singh, D.K., Gaikwad, S., Saswat, T., Chattopadhyay, S., Das, B.K., Tripathy, R., and Ravindran, B. (2019). Cytokine Signature Associated with Disease Severity in Dengue. Viruses, 11.","DOI":"10.3390\/v11010034"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"917","DOI":"10.4269\/ajtmh.22-0780","article-title":"Clinical, Virological, and Immunological Features in Cosmopolitan Genotype DENV-2\u2013Infected Patients during a Large Dengue Outbreak in Sri Lanka in 2017","volume":"109","author":"Nwe","year":"2023","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Guo, C., Zhou, Z., Wen, Z., Liu, Y., Zeng, C., Xiao, D., Ou, M., Han, Y., Huang, S., and Liu, D. (2017). Global Epidemiology of Dengue Outbreaks in 1990\u20132015: A Systematic Review and Meta-Analysis. Front. Cell. Infect. Microbiol., 7.","DOI":"10.3389\/fcimb.2017.00317"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Yamanaka, A., Mulyatno, K.C., Susilowati, H., Hendrianto, E., Ginting, A.P., Sary, D.D., Rantam, F.A., Soegijanto, S., and Konishi, E. (2011). Displacement of the Predominant Dengue Virus from Type 2 to Type 1 with a Subsequent Genotype Shift from IV to I in Surabaya, Indonesia 2008\u20132010. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0027322"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Forshey, B.M., Reiner, R.C., Olkowski, S., Morrison, A.C., Espinoza, A., Long, K.C., Vilcarromero, S., Casanova, W., Wearing, H.J., and Halsey, E.S. (2016). Incomplete Protection against Dengue Virus Type 2 Re-infection in Peru. PLoS Negl. Trop. Dis., 10.","DOI":"10.1371\/journal.pntd.0004398"},{"key":"ref_16","first-page":"219","article-title":"Epidemiological Situation of Dengue Infection in Lao PDR","volume":"35","author":"Fukunaga","year":"1994","journal-title":"Trop. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"55","DOI":"10.5365\/wpsar.2014.5.1.001","article-title":"Sexually transmitted infections among transgender people and men who have sex with men in Port Vila, Vanuatu","volume":"6","author":"Veronese","year":"2015","journal-title":"West. Pac. Surveill. Response"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"21327","DOI":"10.1038\/s41598-024-71807-3","article-title":"Bayesian spatio-temporal analysis of dengue transmission in Lao PDR","volume":"14","author":"Soukavong","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Lao, M., Caro, V., Thiberge, J.-M., Bounmany, P., Vongpayloth, K., Buchy, P., Duong, V., Vanhlasy, C., Hospied, J.-M., and Thongsna, M. (2014). Co-Circulation of Dengue Virus Type 3 Genotypes in Vientiane Capital, Lao PDR. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0115569"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Calvez, E., Pommelet, V., Somlor, S., Pompon, J., Viengphouthong, S., Bounmany, P., Chindavong, T.A., Xaybounsou, T., Prasayasith, P., and Keosenhom, S. (2020). Trends of the Dengue Serotype-4 Circulation with Epidemiological, Phylogenetic, and Entomological Insights in Lao PDR between 2015 and 2019. Pathogens, 9.","DOI":"10.3390\/pathogens9090728"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Calvez, E., Bounmany, P., Bali\u00e8re, C., Somlor, S., Viengphouthong, S., Xaybounsou, T., Keosenhom, S., Fangkham, K., Brey, P.T., and Caro, V. (2021). Using Background Sequencing Data to Anticipate DENV-1 Circulation in the Lao PDR. Microorganisms, 9.","DOI":"10.3390\/microorganisms9112263"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"88","DOI":"10.59096\/osir.v18i2.272340","article-title":"Analysis of the Situation and Factors Associated with Dengue Incidence at the Provincial Level during 2019\u20132023, Lao PDR","volume":"18","author":"Simmalavong","year":"2025","journal-title":"Outbreak Surveill. Investig. Response (OSIR) J."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1186\/s41182-026-01008-3","article-title":"Epidemiological, virological, and clinical characteristics of dengue in the Lao People\u2019s Democratic Republic, 2022\u20132023: A cross-sectional study","volume":"54","author":"Xayavong","year":"2026","journal-title":"Trop. Med. Health"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"17863","DOI":"10.1038\/s41598-022-21722-2","article-title":"Temporal patterns of functional anti-dengue antibodies in dengue infected individuals with different disease outcome or infection history","volume":"12","author":"Vo","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Calvez, E., Somlor, S., Viengphouthong, S., Bali\u00e8re, C., Bounmany, P., Keosenhom, S., Caro, V., and Grandadam, M. (2020). Rapid genotyping protocol to improve dengue virus serotype 2 survey in Lao PDR. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0237384"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1128\/CVI.00396-09","article-title":"Discrepancy in Dengue Virus Neutralizing Antibody Titers between Plaque Reduction Neutralizing Tests with Fc\u03b3 Receptor (Fc\u03b3R)-Negative and Fc\u03b3R-Expressing BHK-21 Cells","volume":"17","author":"Moi","year":"2010","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1126\/science.1185181","article-title":"Cross-Reacting Antibodies Enhance Dengue Virus Infection in Humans","volume":"328","author":"Dejnirattisai","year":"2010","journal-title":"Science"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.ijid.2023.11.025","article-title":"Role of pre-existing immunity in driving the dengue virus serotype 2 genotype shift in the Philippines: A retrospective analysis of serological data","volume":"139","author":"Balingit","year":"2023","journal-title":"Int. J. Infect. Dis."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Troupin, C., Intavong, K., Somlor, S., Viengphouthong, S., Keosenhom, S., Chindavong, T.A., Bounmany, P., Vachouaxiong, L., Xaybounsou, T., and Vanhnollat, C. (2025). Molecular Epidemiology of Dengue Viruses in Lao People\u2019s Democratic Republic, 2020\u20132023. Microorganisms, 13.","DOI":"10.3390\/microorganisms13020318"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/s00284-020-02284-w","article-title":"Current Understanding of the Pathogenesis of Dengue Virus Infection","volume":"78","author":"Bhatt","year":"2020","journal-title":"Curr. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"796","DOI":"10.4269\/ajtmh.2005.73.796","article-title":"Molecular epidemiology of dengue 2 viruses in the Philippines: Genotype shift and local evolution","volume":"73","author":"Salda","year":"2005","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1086\/315215","article-title":"Dengue Viremia Titer, Antibody Response Pattern, and Virus Serotype Correlate with Disease Severity","volume":"181","author":"Vaughn","year":"2000","journal-title":"J. Infect. Dis."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Samune, Y., Saito, A., Sasaki, T., Koketsu, R., Srimark, N., Phadungsombat, J., Yokoyama, M., Kotani, O., Sato, H., and Yamanaka, A. (2024). Genetic regions affecting the replication and pathogenicity of dengue virus type 2. PLoS Neglected Trop. Dis., 18.","DOI":"10.1371\/journal.pntd.0011885"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"ofae753","DOI":"10.1093\/ofid\/ofae753","article-title":"Epidemiology and Genotype Dynamics of Dengue in Hospitalized Patients in Northern Vietnam Between 2020 and 2022","volume":"12","author":"Anh","year":"2024","journal-title":"Open Forum Infect. Dis."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1038\/s42003-025-07999-9","article-title":"Climate, inter-serotype competition and arboviral interactions shape dengue dynamics in Thailand","volume":"8","author":"Perez","year":"2025","journal-title":"Commun. Biol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"30","DOI":"10.5365\/wpsar.2022.13.4.932","article-title":"Epidemiological profile of dengue in Champasak and Savannakhet provinces, Lao People\u2019s Democratic Republic, 2003\u20132020","volume":"13","author":"Zafar","year":"2022","journal-title":"West. Pac. Surveill. Response"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"105815","DOI":"10.1016\/j.meegid.2025.105815","article-title":"Genomic characterization and serotype distribution of dengue virus circulating in Pakistan during 2024","volume":"134","author":"Jamal","year":"2025","journal-title":"Infect. Genet. Evol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e0118324","DOI":"10.1128\/jvi.01183-24","article-title":"Envelope domain III E324, E351, and E380 mutations lever adaptive evolution of DENV-1 genotype I","volume":"98","author":"Jiang","year":"2024","journal-title":"J. Virol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Afreen, N., Naqvi, I.H., Broor, S., Ahmed, A., Kazim, S.N., Dohare, R., Kumar, M., and Parveen, S. (2016). Evolutionary Analysis of Dengue Serotype 2 Viruses Using Phylogenetic and Bayesian Methods from New Delhi, India. PLoS Neglected Trop. Dis., 10.","DOI":"10.1371\/journal.pntd.0004511"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/0166-0934(85)90120-X","article-title":"Antibody-capture ELISA for detection of immunoglobulin M antibodies in sera from japanese encephalitis and dengue hemorrhagic fever patients","volume":"11","author":"Bundo","year":"1985","journal-title":"J. Virol. Methods"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1089\/vbz.2008.0153","article-title":"Evaluation of a Dengue IgG Indirect Enzyme-Linked Immunosorbent Assay and a Japanese Encephalitis IgG Indirect Enzyme-Linked Immunosorbent Assay for Diagnosis of Secondary Dengue Virus Infection","volume":"10","author":"Inoue","year":"2009","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.meegid.2016.04.025","article-title":"Characterization of the 2013 dengue epidemic in Myanmar with dengue virus 1 as the dominant serotype","volume":"43","author":"Tun","year":"2016","journal-title":"Infect. Genet. Evol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Tun, M.M.N., Kapandji, M., Wada, A., Yamamoto, K., Dumre, S.P., Nwe, K.M., Lin, H., Takamatsu, Y., Thant, K.Z., and Thu, H.M. (2024). Performance of Fujifilm Dengue NS1 Antigen Rapid Diagnosis Kit Compared to Quantitative Real-Time Polymerase Chain Reaction. Pathogens, 13.","DOI":"10.3390\/pathogens13090818"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Fraenkel, S., Nabeshima, T., Xayavong, D., Nguyen, T.T.N., Xu, Q., Kapandji, M., Yamao, K., Balingit, J.C., Pandey, B.D., and Morita, K. (2024). The Development of New Primer Sets for the Amplification and Sequencing of the Envelope Gene of All Dengue Virus Serotypes. Microorganisms, 12.","DOI":"10.3390\/microorganisms12061092"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1658","DOI":"10.1093\/bioinformatics\/btl158","article-title":"Cd-hit: A fast program for clustering and comparing large sets of protein or nucleotide sequences","volume":"22","author":"Li","year":"2006","journal-title":"Bioinformatics"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability","volume":"30","author":"Katoh","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"msae263","DOI":"10.1093\/molbev\/msae263","article-title":"MEGA12: Molecular Evolutionary Genetic Analysis Version 12 for Adaptive and Green Computing","volume":"41","author":"Kumar","year":"2024","journal-title":"Mol. Biol. Evol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1099\/vir.0.058008-0","article-title":"Production of single-round infectious chimeric flaviviruses with DNA-based Japanese encephalitis virus replicon","volume":"95","author":"Suzuki","year":"2014","journal-title":"J. Gen. Virol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-018-34865-y","article-title":"High-throughput neutralization assay for multiple flaviviruses based on single-round infectious particles using dengue virus type 1 reporter replicon","volume":"8","author":"Matsuda","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1038\/s41541-025-01204-y","article-title":"Cross-genotype immunogenicity and antibody-dependent enhancement of KD-382 dengue vaccine in flavivirus-na\u00efve adults","volume":"10","author":"Balingit","year":"2025","journal-title":"npj Vaccines"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/19\/8869\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:15:37Z","timestamp":1791346537000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/19\/8869"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,5]]},"references-count":50,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2026,10]]}},"alternative-id":["ijms27198869"],"URL":"https:\/\/doi.org\/10.3390\/ijms27198869","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,5]]}}}