{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T01:51:59Z","timestamp":1791597119793,"version":"4.3.4"},"reference-count":39,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>This prospective single-center cohort study evaluated changes in serum levels of novel adipokines and myokines, including isthmin-1 and irisin, as well as the advanced glycation end product (AGE)\u2013soluble receptor for AGE (sRAGE) axis and monomeric C-reactive protein (mCRP) in patients with obesity before and 5 months after laparoscopic sleeve gastrectomy (LSG). The study included 38 patients with obesity (26 females and 12 males) with a median age of 42 years who underwent LSG. Laboratory parameters were assessed using commercially available immunoenzymatic assays. We observed a significant decrease in isthmin-1 concentration at the postoperative time point (Me = 2.10 ng\/mL vs. Me = 1.93 ng\/mL, p = 0.009). Moreover, a significant increase in irisin levels (Me = 7.33 \u00b5g\/mL vs. Me = 10.64 \u00b5g\/mL, p = 0.002) and AGEs (Me = 589.50 ng\/mL vs. Me = 698.60 ng\/mL, p = 0.015) was observed after LSG. A significant increase in AGE levels was also noted after normalization to total protein (Me = 8.34 ng\/mg protein vs. Me = 10.37 ng\/mg protein, p = 0.027). sRAGE levels were similar at both time points. Five months after LSG, mCRP levels were significantly lower (Me = 1.99 ng\/mL vs. Me = 1.55 ng\/mL, p = 0.020). Exploratory correlation analyses identified potential associations between AGEs and isthmin-1 (R = 0.37) and between sRAGE and irisin (R = \u22120.31) preoperatively. Five months after LSG, potential associations were observed between AGEs and irisin (R = 0.38) and between sRAGE and mCRP (R = \u22120.37). Our findings indicate a decrease in circulating isthmin-1 and mCRP, together with an increase in irisin and AGE concentrations following LSG, suggesting changes in selected metabolic and tissue-related biomarkers after surgery. Further studies are needed to determine the mechanisms underlying these changes and to clarify their potential clinical significance.<\/jats:p>","DOI":"10.3390\/ijms27198932","type":"journal-article","created":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T01:40:17Z","timestamp":1791596417000},"page":"8932","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Changes\u00a0in Isthmin-1, Irisin, and the AGE\u2013RAGE Axis Following LSG: A Prospective Single-Center Cohort Study"],"prefix":"10.3390","volume":"27","author":[{"given":"Wojciech","family":"Kupczyk","sequence":"first","affiliation":[{"name":"Department of General, Gastroenterological, and Oncological Surgery, Faculty of Medicine, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toru\u0144, 87-100 Toru\u0144, Poland"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"}]},{"given":"Joanna","family":"Boinska","sequence":"additional","affiliation":[{"name":"Pathophysiology Department, Faculty of Pharmacy, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toru\u0144, 87-100 Toru\u0144, Poland"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4137-2981","authenticated-orcid":false,"given":"Artur","family":"S\u0142omka","sequence":"additional","affiliation":[{"name":"Pathophysiology Department, Faculty of Pharmacy, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toru\u0144, 87-100 Toru\u0144, Poland"},{"name":"Department of Hematology and Oncology, National Medical Institute of the Ministry of Interior and Administration, 02-507 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Kinga","family":"Kupczyk","sequence":"additional","affiliation":[{"name":"Department of General, Gastroenterological, and Oncological Surgery, Faculty of Medicine, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toru\u0144, 87-100 Toru\u0144, Poland"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Marek","family":"Jackowski","sequence":"additional","affiliation":[{"name":"Department of General, Gastroenterological, and Oncological Surgery, Faculty of Medicine, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toru\u0144, 87-100 Toru\u0144, Poland"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"supervision","vocabulary":"credit"}]},{"given":"Ewa","family":"\u017bekanowska","sequence":"additional","affiliation":[{"name":"Pathophysiology Department, Faculty of Pharmacy, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toru\u0144, 87-100 Toru\u0144, Poland"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,10,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1016\/j.soard.2022.08.013","article-title":"2022 American Society for Metabolic and Bariatric Surgery (ASMBS) and International Federation for the Surgery of Obesity and Metabolic Disorders (IFSO): Indications for Metabolic and Bariatric Surgery","volume":"18","author":"Eisenberg","year":"2022","journal-title":"Surg. 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