{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:45:23Z","timestamp":1760060723379,"version":"build-2065373602"},"reference-count":22,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T00:00:00Z","timestamp":1758240000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJNS"],"abstract":"<jats:p>The State Hygienic Lab at the University of Iowa (SHL) performs newborn blood spot screening (NBS) for IA, AK, ND, and SD. In October 2022, we halted in-house CFTR DNA testing due to the unexpected nonperformance of our newly expanded variant panel. Samples were sent to a reference laboratory to ensure uninterrupted testing and by December 2022, SHL had selected an alternative test that enabled CFTR panel expansion as envisioned. However, due to circumstances beyond our control, test implementation was severely delayed, and in-house testing was paused. These events were consequential. Firstly, our prolonged utilization of reference labs and fees was a financial strain on the lab. Secondly, our timeliness decreased significantly, and lastly, these issues were burdensome for staff. The lab overcame these problems using three strategies: effective communication; technical expertise; and staff perseverance. Finally, in Aug 2023, SHL successfully resumed in-house testing. As state labs ponder major CFTR algorithm changes, such as the addition of next generation sequencing, the strategies we utilized can be useful during sudden setbacks. Our experience of replacing our CFTR assay underscores the importance of emergency preparedness and partnership within the NBS community.<\/jats:p>","DOI":"10.3390\/ijns11030082","type":"journal-article","created":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T12:08:03Z","timestamp":1758542883000},"page":"82","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Impact of Cystic Fibrosis Algorithm Changes: A Case Study of Challenges and Strategies"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4550-6924","authenticated-orcid":false,"given":"Jerusalem","family":"Alleyne","sequence":"first","affiliation":[{"name":"State Hygienic Laboratory, University of Iowa, Coralville, IA 52241, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth","family":"Coursey","sequence":"additional","affiliation":[{"name":"State Hygienic Laboratory, University of Iowa, Coralville, IA 52241, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimberly","family":"Noble Piper","sequence":"additional","affiliation":[{"name":"Iowa Department of Health and Human Services, Des Moines, IA 50319, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cynthia","family":"Cass","sequence":"additional","affiliation":[{"name":"State Hygienic Laboratory, University of Iowa, Coralville, IA 52241, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4830-4383","authenticated-orcid":false,"given":"Michael","family":"Pentella","sequence":"additional","affiliation":[{"name":"State Hygienic Laboratory, University of Iowa, Coralville, IA 52241, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1016\/j.pcl.2023.06.003","article-title":"Newborn Screening","volume":"70","author":"Pappas","year":"2023","journal-title":"Pediatr. Clin. N. Am."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Gaviglio, A., McKasson, S., Singh, S., and Ojodu, J. (2023). Infants with Congenital Diseases Identified through Newborn Screening\u2014United States, 2018\u20132020. Int. J. Neonatal Screen., 9.","DOI":"10.3390\/ijns9020023"},{"key":"ref_3","unstructured":"(2025, June 19). Recommended Uniform Screening Panel|HRSA, Available online: https:\/\/www.hrsa.gov\/advisory-committees\/heritable-disorders\/rusp."},{"key":"ref_4","unstructured":"(2025, June 19). Intro to CF|Cystic Fibrosis Foundation. Available online: https:\/\/www.cff.org\/intro-cf."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1038\/s41572-024-00538-6","article-title":"Cystic Fibrosis","volume":"10","author":"Mall","year":"2024","journal-title":"Nat. Rev. Dis. Primer"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1016\/S2213-2600(16)00053-9","article-title":"Newborn Screening for Cystic Fibrosis","volume":"4","author":"Castellani","year":"2016","journal-title":"Lancet Respir. Med."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Travert, G., Heeley, M., and Heeley, A. (2020). History of Newborn Screening for Cystic Fibrosis\u2014The Early Years. Int. J. Neonatal Screen., 6.","DOI":"10.3390\/ijns6010008"},{"key":"ref_8","unstructured":"(2025, June 19). Iowa Newborn Screening Program|Health & Human Services, Available online: https:\/\/hhs.iowa.gov\/programs-and-services\/family-health\/congenital-inherited-disorders\/iowa-newborn-screening-program."},{"key":"ref_9","unstructured":"(2025, June 19). State Hygienic Laboratory|The University of Iowa. Available online: https:\/\/shl.uiowa.edu\/."},{"key":"ref_10","unstructured":"(2025, June 19). 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Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1001\/jama.2024.19662","article-title":"Expanded Newborn Screening Using Genome Sequencing for Early Actionable Conditions","volume":"333","author":"Ziegler","year":"2025","journal-title":"JAMA"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"McGarry, M.E., Raraigh, K.S., Farrell, P., Shropshire, F., Padding, K., White, C., Dorley, M.C., Hicks, S., Ren, C.L., and Tullis, K. (2025). Cystic Fibrosis Newborn Screening: A Systematic Review-Driven Consensus Guideline from the United States Cystic Fibrosis Foundation. Int. J. Neonatal Screen., 11.","DOI":"10.3390\/ijns11020024"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Dwight, M., and Faro, A. (2025). It Takes All of Us: How the Cystic Fibrosis Foundation Is Supporting States in Advancing Cystic Fibrosis Newborn Screening. Int. J. 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