{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T17:33:21Z","timestamp":1771263201524,"version":"3.50.1"},"reference-count":34,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T00:00:00Z","timestamp":1690243200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fondazione per la Ricerca sulla Fibrosi Cistica","award":["FFC#30\/2018"],"award-info":[{"award-number":["FFC#30\/2018"]}]},{"name":"Fondazione per la Ricerca sulla Fibrosi Cistica","award":["FFC#24\/2020"],"award-info":[{"award-number":["FFC#24\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJNS"],"abstract":"<jats:p>The sweat test (ST) is the current diagnostic gold standard for cystic fibrosis (CF). Many CF centres have switched from the Gibson\u2013Cooke method to the Macroduct system-based method. We used these methods simultaneously to compare CF screening outcomes. STs using both methods were performed simultaneously between March and December 2022 at CF Centre in Florence. We included newborns who underwent newborn bloodspot screening (NBS), newborns undergoing transfusion immediately after birth, and children with CF screen-positive, inconclusive diagnosis (CFSPID). We assessed 72 subjects (median age 4.4 months; range 0\u201376.7): 30 (41.7%) NBS-positive, 18 (25.0%) newborns who underwent transfusion, and 24 (33.3%) children with CFSPID. No significant differences were found between valid sample numbers, by patient ages and groups (p = 0.10) and between chloride concentrations (p = 0.13), except for sweat chloride (SC) measured by the Gibson\u2013Cooke and Macroduct methods in CFSPID group (29.0, IQR: 20.0\u201348.0 and 22.5, IQR: 15.5\u201330.8, respectively; p = 0.01). The Macroduct and Gibson\u2013Cooke methods showed substantial agreement with the SC values, except for CFSPID, whose result may depend on the method of sweat collection. In case of invalid values with Macroduct, the test should be repeated with Gibson\u2013Cooke method.<\/jats:p>","DOI":"10.3390\/ijns9030041","type":"journal-article","created":{"date-parts":[[2023,7,26]],"date-time":"2023-07-26T01:09:01Z","timestamp":1690333741000},"page":"41","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Comparison between Gibson\u2013Cooke and Macroduct Methods in the Cystic Fibrosis Neonatal Screening Program and in Subjects Who Are Cystic Fibrosis Screen-Positive with an Inconclusive Diagnosis"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8317-9928","authenticated-orcid":false,"given":"Daniela","family":"Dolce","sequence":"first","affiliation":[{"name":"Cystic Fibrosis Regional Reference Center, Department of Paediatric Medicine, Meyer Children\u2019s Hospital IRCCS, Viale Gaetano Pieraccini 24, 50139 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3642-8928","authenticated-orcid":false,"given":"Cristina","family":"Fevola","sequence":"additional","affiliation":[{"name":"Cystic Fibrosis Regional Reference Center, Department of Paediatric Medicine, Meyer Children\u2019s Hospital IRCCS, Viale Gaetano Pieraccini 24, 50139 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erica","family":"Camera","sequence":"additional","affiliation":[{"name":"Cystic Fibrosis Regional Reference Center, Department of Paediatric Medicine, Meyer Children\u2019s Hospital IRCCS, Viale Gaetano Pieraccini 24, 50139 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3724-7501","authenticated-orcid":false,"given":"Tommaso","family":"Orioli","sequence":"additional","affiliation":[{"name":"Cystic Fibrosis Regional Reference Center, Department of Paediatric Medicine, Meyer Children\u2019s Hospital IRCCS, Viale Gaetano Pieraccini 24, 50139 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5608-5902","authenticated-orcid":false,"given":"Ersilia","family":"Lucenteforte","sequence":"additional","affiliation":[{"name":"Unit of Medical Statistics, Department of Clinical and Experimental Medicine, University of Pisa, 56126 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco Andrea","family":"Malanima","sequence":"additional","affiliation":[{"name":"Unit of Medical Statistics, Department of Clinical and Experimental Medicine, University of Pisa, 56126 Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9448-5381","authenticated-orcid":false,"given":"Giovanni","family":"Taccetti","sequence":"additional","affiliation":[{"name":"Cystic Fibrosis Regional Reference Center, Department of Paediatric Medicine, Meyer Children\u2019s Hospital IRCCS, Viale Gaetano Pieraccini 24, 50139 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1106-4424","authenticated-orcid":false,"given":"Vito","family":"Terlizzi","sequence":"additional","affiliation":[{"name":"Cystic Fibrosis Regional Reference Center, Department of Paediatric Medicine, Meyer Children\u2019s Hospital IRCCS, Viale Gaetano Pieraccini 24, 50139 Florence, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/S2213-2600(19)30337-6","article-title":"The Future of Cystic Fibrosis Care: A Global Perspective","volume":"8","author":"Bell","year":"2020","journal-title":"Lancet Respir. 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