{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T12:08:58Z","timestamp":1780056538516,"version":"3.54.0"},"reference-count":62,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2021,3,30]],"date-time":"2021-03-30T00:00:00Z","timestamp":1617062400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004493","name":"International Atomic Energy Agency","doi-asserted-by":"publisher","award":["22628\/R0"],"award-info":[{"award-number":["22628\/R0"]}],"id":[{"id":"10.13039\/501100004493","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004493","name":"International Atomic Energy Agency","doi-asserted-by":"publisher","award":["project BRA0023"],"award-info":[{"award-number":["project BRA0023"]}],"id":[{"id":"10.13039\/501100004493","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>A common strategy used to maintain sterile fly quality without sacrificing sterility is to irradiate the insects under an oxygen-reduced atmosphere. So far, sterilizing doses for the South American fruit fly Anastrepha fraterculus have only been determined under normoxia. Our study reports for the first time the dose-sterility response under hypoxia for two different A. fraterculus strains. The pupae were derived from a bisexual strain (a Brazilian-1 population) and a recently developed genetic sexing strain (GSS-89). Two hours prior to irradiation, pupae were transferred to sealed glass bottles and irradiated when oxygen concentration was below 3%. Four types of crosses with nonirradiated flies of the bisexual strain were set to assess sterility for each radiation dose. For males from both strains, Weibull dose\u2013response curves between radiation doses and the proportion of egg hatch, egg-to-pupa recovery, and recovery of adults were determined. The GSS males revealed high sterility\/mortality levels compared to males from the bisexual strain at doses &lt; 40 Gy, but a dose of 74 Gy reduced egg hatch by 99% regardless of the male strain and was considered the sterilizing dose. The fertility of irradiated females was severely affected even at low doses under hypoxia.<\/jats:p>","DOI":"10.3390\/insects12040308","type":"journal-article","created":{"date-parts":[[2021,3,31]],"date-time":"2021-03-31T00:13:10Z","timestamp":1617149590000},"page":"308","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Determining the Sterilization Doses under Hypoxia for the Novel Black Pupae Genetic Sexing Strain of Anastrepha fraterculus (Diptera, Tephritidae)"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6639-0994","authenticated-orcid":false,"given":"Paloma Della","family":"Giustina","sequence":"first","affiliation":[{"name":"Center for Nuclear Energy in Agriculture (CENA\/USP), Piracicaba, S\u00e3o Paulo 13416-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1882-9565","authenticated-orcid":false,"given":"Thiago","family":"Mastrangelo","sequence":"additional","affiliation":[{"name":"Center for Nuclear Energy in Agriculture (CENA\/USP), Piracicaba, S\u00e3o Paulo 13416-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sohel","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Insect Pest Control Laboratory, Joint FAO\/IAEA Centre of Nuclear Techniques in Food and Agriculture, A-2444 Seibersdorf, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Mascarin","sequence":"additional","affiliation":[{"name":"EMBRAPA Environment, Jaguari\u00fana, S\u00e3o Paulo 13918-110, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Caceres","sequence":"additional","affiliation":[{"name":"Insect Pest Control Laboratory, Joint FAO\/IAEA Centre of Nuclear Techniques in Food and Agriculture, A-2444 Seibersdorf, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1590\/S1519-566X2004000100015","article-title":"Fruit fly (Diptera: Tephritoidea) infestation in citrus in the State of S\u00e3o Paulo, Brazil","volume":"33","author":"Raga","year":"2004","journal-title":"Neotrop. Entomol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Prezotto, L., Perondini, A., Hern\u00e1ndez-Ortiz, V., Fr\u00edas, D., and Selivon, D. (2019). What Can Integrated Analysis of Morphological and Genetic Data Still Reveal about the Anastrepha fraterculus (Diptera: Tephritidae) Cryptic Species Complex?. Insects, 10.","DOI":"10.3390\/insects10110408"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"P\u00e9rez-Staples, D., D\u00edaz-Fleischer, F., Montoya, P., and Vera, M. (2019). A Review of the Natural Host Plants of the Anastrepha fraterculus Complex in the Americas. Area-Wide Management of Fruit Fly Pests, CRC Press.","DOI":"10.1201\/9780429355738"},{"key":"ref_4","unstructured":"Fundecitrus (2020, November 20). Fundo de Defesa da Citricultura. Estimativa da safra de laranja 2020\/2021 do Cintur\u00e3o Citr\u00edcola de S\u00e3o Paulo e Tri\u00e2ngulo\/Sudoeste Mineiro: Sum\u00e1rio Executivo 2020\/21. Available online: Fundecitrus.com.br."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Cladera, J., Vilardi, J., Juri, M., Paulin, L., Giardini, M., G\u00f3mez Cendra, P., Segura, D., and Lanzavecchia, S. (2014). Genetics and biology of Anastrepha fraterculus: Research supporting the use of the sterile insect technique (SIT) to control this pest in Argentina. BMC Genet., 15.","DOI":"10.1186\/1471-2156-15-S2-S12"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1653\/0015-4040(2007)90[53:DAQCPO]2.0.CO;2","article-title":"Demographic and quality control parameters of Anastrepha fraterculus (Diptera: Tephritidae) maintained under artificial rearing","volume":"90","author":"Vera","year":"2007","journal-title":"Fla. Entomol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1590\/0103-9016-2013-233","article-title":"Large scale artificial rearing of Anastrepha sp.1 aff. fraterculus (Diptera: Tephritidae) in Brazil","volume":"71","author":"Walder","year":"2014","journal-title":"Sci. Agric."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Dyck, V.A., Hendrichs, J., and Robinson, A.S. (2021). Sterilizing insects with ionizing radiation. Sterile Insect Technique: Principles and Practice in Area-Wide Integrated Pest Management, CRC Press. [2nd ed.].","DOI":"10.1201\/9781003035572"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/S1383-5742(02)00006-6","article-title":"Mutations and their use in insect control","volume":"511","author":"Robinson","year":"2002","journal-title":"Mutat. Res. Rev. Mutat."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Dyck, V.A., Hendrichs, J., and Robinson, A.S. (2021). Sterile Insect Quality Control\/Assurance. Sterile Insect Technique: Principles and Practice in Area-Wide Integrated Pest Management, CRC Press. [2nd ed.].","DOI":"10.1201\/9781003035572"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1653\/0015-4040(2007)90[88:SITAMF]2.0.CO;2","article-title":"Sterile insect technique: A model for dose optimization for improved sterile insect quality","volume":"90","author":"Parker","year":"2007","journal-title":"Fla. Entomol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Nenoi, M. (2015). Ionizing Radiations in Entomology. Evolution of Ionizing Radiation Research, InTechOpen.","DOI":"10.5772\/59330"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"L\u00f3pez-Mart\u00ednez, G., and Hahn, D. (2014). Early Life Hormetic Treatments Decrease Irradiation-Induced Oxidative Damage, Increase Longevity, and Enhance Sexual Performance during Old Age in the Caribbean Fruit Fly. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0088128"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1093\/jee\/tox326","article-title":"Sexual Competitiveness, Field Survival, and Dispersal of Anastrepha obliqua (Diptera: Tephritidae) Fruit Flies Irradiated at Different Doses","volume":"111","author":"Liedo","year":"2018","journal-title":"J. Econ. Entomol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1590\/S0103-90161993000200001","article-title":"Effects of gamma radiation on the sterility and behavioral quality of the caribbean fruit fly, Anastrepha suspensa (Loew) (Diptera:Tephritidae)","volume":"50","author":"Walder","year":"1993","journal-title":"Sci. Agr\u00edcola"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1653\/0015-4040(2007)90[96:IOSIAF]2.0.CO;2","article-title":"Induction of sterility in Anastrepha fraterculus (Diptera: Tephritidae) by gamma radiation","volume":"90","author":"Allinghi","year":"2007","journal-title":"Fla. Entomol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1111\/j.1570-7458.2011.01196.x","article-title":"Quantity and safety vs. quality and performance: Conflicting interests during mass rearing and transport affect the efficiency of sterile insect technique programs","volume":"142","author":"Rull","year":"2011","journal-title":"Entomol. Exp. Appl."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1673\/031.012.4501","article-title":"Mass Rearing History and Irradiation Affect Mating Performance of the Male Fruit Fly, Anastrepha obliqua","volume":"12","author":"Rull","year":"2012","journal-title":"J. Insect Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"S28","DOI":"10.1017\/S174275841400006X","article-title":"Improved mating performance of male Anastrepha ludens (Diptera: Tephritidae) irradiated at low doses for release in sterile insect technique programmes","volume":"34","author":"Rull","year":"2014","journal-title":"Int. J. Trop. Insect Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1068","DOI":"10.1093\/jee\/64.5.1068","article-title":"Competitiveness of Gamma-Sterilized Males of the Mediterranean Fruit Fly1","volume":"64","author":"Hooper","year":"1971","journal-title":"J. Econ. Entomol."},{"key":"ref_21","unstructured":"Zavala-L\u00f3pez, J.L., and Enkerlin, W.R. (2017). Guideline for Packing, Shipping, Holding and Release of Sterile Flies in Area-Wide Fruit Fly Control Programmes, Food and Agriculture Organization of the United Nations. [2nd ed.]."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1080\/09583157.2016.1145627","article-title":"Effect of hypoxia and its repercussions in packing pupae of the parasitoid Diachasmimorpha longicaudata (Hymenoptera: Braconidae) for shipment","volume":"26","author":"Cancino","year":"2016","journal-title":"Biocontrol. Sci. Technol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Dyck, V.A., Hendrichs, J., and Robinson, A.S. (2021). Supply, Emergence, and Release of Sterile Insects. Sterile Insect Technique: Principles and Practice in Area-Wide Integrated Pest Management, CRC Press. [2nd ed.].","DOI":"10.1201\/9781003035572"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Mastrangelo, T., Kovaleski, A., Botteon, V., Scopel, W., and Costa, M. (2018). Optimization of the sterilizing doses and overflooding ratios for the South American fruit fly. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0201026"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Dias, V., Hallman, G., Mart\u00ednez-Barrera, O., Hurtado, N., Cardoso, A., Parker, A., Caravantes, L., Rivera, C., Ara\u00fajo, A., and Maxwell, F. (2020). Modified Atmosphere Does Not Reduce the Efficacy of Phytosanitary Irradiation Doses Recommended for Tephritid Fruit Flies. Insects, 11.","DOI":"10.3390\/insects11060371"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1603\/0022-0493-97.5.1547","article-title":"Medfly (Diptera:Tephritidae) Genetic Sexing: Large-Scale Field Comparison of Males-Only and Bisexual Sterile Fly Releases in Guatemala","volume":"97","author":"McInnis","year":"2004","journal-title":"J. Econ. Entomol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1111\/j.1439-0418.2012.01748.x","article-title":"Do sterile females affect the sexual performance of sterile males of Anastrepha ludens (Diptera: Tephritidae)?","volume":"137","author":"Orozco","year":"2012","journal-title":"J. Appl. Entomol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Zepeda-Cisneros, C., Meza Hern\u00e1ndez, J., Garc\u00eda-Mart\u00ednez, V., Iba\u00f1ez-Palacios, J., Zacharopoulou, A., and Franz, G. (2014). Development, genetic and cytogenetic analyses of genetic sexing strains of the Mexican fruit fly, Anastrepha ludens Loew (Diptera: Tephritidae). BMC Genet., 15.","DOI":"10.1186\/1471-2156-15-S2-S1"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Meza, J., Bourtzis, K., Zacharopoulou, A., Gariou-Papalexiou, A., and C\u00e1ceres, C. (2020). Development and characterization of a pupal-colour based genetic sexing strain of Anastrepha fraterculus sp. 1 (Diptera: Tephritidae). BMC Genet., 21.","DOI":"10.1186\/s12863-020-00932-5"},{"key":"ref_30","unstructured":"Mitchell, S., Tanaka, N., and Steiner, L.F. (1965). Methods of Mass Culturing Melon Flies and Oriental and Mediterranean Fruit Flies, U.S. Dept. of Agriculture, Agricultural Research Service."},{"key":"ref_31","unstructured":"International Atomic Energy Agency (2020, December 10). Dosimetry System for SIT: Standard Operating Procedure for Gafchromic \u00delm. Available online: http:\/\/www.naweb.iaea.org\/nafa\/ipc\/public\/ipc-gafchromic-dosimetry-sit.html."},{"key":"ref_32","unstructured":"Food and Agriculture Organization of the United Nations, International Atomic Energy Agency, and United States Department of Agriculture (2019). Product Quality Control for Sterile Mass-Reared and Released Tephritid Fruit Flies, Version 7.0."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Ritz, C., Baty, F., Streibig, J., and Gerhard, D. (2015). Dose-Response Analysis Using R. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0146021"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Wickham, H. (2016). ggplot2\u2014Elegant Graphics for Data Analysis, Springer. [2nd ed.].","DOI":"10.1007\/978-3-319-24277-4_9"},{"key":"ref_35","unstructured":"R Core Team (2020, December 10). R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing. Available online: http:\/\/www.R-project.org\/."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.cbpa.2019.04.004","article-title":"Twenty years of the \u2018Preparation for Oxidative Stress\u2019 (POS) theory: Ecophysiological advantages and molecular strategies","volume":"234","author":"Moreira","year":"2019","journal-title":"Comp. Biochem. Phys. A"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1146\/annurev-ento-020117-043145","article-title":"Functional Hypoxia in Insects: Definition, Assessment, and Consequences for Physiology, Ecology, and Evolution","volume":"63","author":"Harrison","year":"2018","journal-title":"Annu. Rev. Entomol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"110658","DOI":"10.1016\/j.cbpa.2020.110658","article-title":"A dose of experimental hormesis: When mild stress protects and improves animal performance","volume":"242","author":"Berry","year":"2020","journal-title":"Comp. Biochem. Phys. A"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2150","DOI":"10.1242\/jeb.065631","article-title":"Short-term anoxic conditioning hormesis boosts antioxidant defenses, lowers oxidative damage following irradiation and enhances male sexual performance in the Caribbean fruit fly, Anastrepha suspensa","volume":"215","author":"Hahn","year":"2012","journal-title":"J. Exp. Bio."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1603\/EC13370","article-title":"Low-Oxygen Atmospheric Treatment Improves the Performance of Irradiation-Sterilized Male Cactus Moths Used in SIT","volume":"107","author":"Carpenter","year":"2014","journal-title":"J. Econ. Entomol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1093\/jee\/70.2.165","article-title":"Mediterranean Fruit Flies: Sterility and Sexual Competitiveness in the Laboratory After Treatment with Gamma Irradiation in Air, Carbon Dioxide, Helium, Nitrogen or Partial Vacuum1","volume":"70","author":"Ohinata","year":"1977","journal-title":"J. Econ. Entomol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1609","DOI":"10.1093\/jee\/90.6.1609","article-title":"Irradiation Effects in Air and in Nitrogen on Mediterranean Fruit Fly (Diptera: Tephritidae) Pupae in Western Australia","volume":"90","author":"Fisher","year":"1997","journal-title":"J. Econ. Entomol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1023\/A:1020967810703","article-title":"Mass rearing of temperature sensitive genetic sexing strains in the Mediterranean fruit fly (Ceratitis capitata)","volume":"116","author":"Caceres","year":"2002","journal-title":"Genetica"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Orozco-D\u00e1vila, D., Adriano-Anaya, M., Quintero-Fong, L., and Salvador-Figueroa, M. (2015). Sterility and Sexual Competitiveness of Tapachula-7 Anastrepha ludens Males Irradiated at Different Doses. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0135759"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"526","DOI":"10.2307\/3574127","article-title":"Influence of Anoxia during Gamma Irradiation on the Fertility and Competitiveness of the Adult Male Codling Moth, Laspeyresia pomonella (L.)","volume":"61","author":"Robinson","year":"1975","journal-title":"Radiat. Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1111\/j.1570-7458.1974.tb00323.x","article-title":"The viability, fertility and behaviour of tsetse flies (Glossina morsitans) sterilized by irradiation under various conditions","volume":"17","author":"Langley","year":"1974","journal-title":"Entomol. Exp. Appl."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Sass\u00f9, F., Nikolouli, K., Pereira, R., Vreysen, M., Stauffer, C., and C\u00e1ceres, C. (2019). Irradiation dose response under hypoxia for the application of the sterile insect technique in Drosophila suzukii. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0226582"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1093\/jee\/56.1.42","article-title":"Effects of Gamma Radiation on Various Stages of Three Fruit Fly Species","volume":"56","author":"Balock","year":"1963","journal-title":"J. Econ. Entomol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1093\/aesa\/78.1.101","article-title":"Gamma Irradiation of the Mediterranean Fruit Fly (Diptera: Tephritidae): Effects of Puparial Age under Induced Hypoxia on Female Sterility","volume":"78","author":"Williamson","year":"1985","journal-title":"Ann. Entomol. Soc. Am."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1653\/0015-4040(2007)90[80:EOPCOM]2.0.CO;2","article-title":"Effects of pre-irradiation conditioning of Medfly pupae (Diptera: Tephritidae): Hypoxia and quality of sterile males","volume":"90","author":"Nestel","year":"2007","journal-title":"Fla. Entomol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1603\/EC09139","article-title":"A New Generation of X Ray Irradiators for Insect Sterilization","volume":"103","author":"Mastrangelo","year":"2010","journal-title":"J. Econ. Entomol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.1093\/jee\/tox119","article-title":"Effect of Irradiation Doses on Sterility and Biological Security in a Genetically Modified Strain of the Mediterranean Fruit Fly (Diptera: Tephritidae)","volume":"110","author":"Toledo","year":"2017","journal-title":"J. Econ. Entomol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1007\/s42690-019-00039-0","article-title":"Biochemical patterns of protein in gamma irradiated males of Ceratitis capitata (Wied.)","volume":"39","author":"Sayed","year":"2019","journal-title":"Int. J. Trop. Insect Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1093\/jee\/84.4.1227","article-title":"Mating Competitiveness of Irradiated Mexican Fruit Fly (Diptera: Tephritidae)","volume":"84","author":"Moreno","year":"1991","journal-title":"J. Econ. Entomol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1653\/024.100.0312","article-title":"Timing of Irradiation and Male Mating History Effects on Female Remating in Anastrepha ludens (Diptera: Tephritidae)","volume":"100","author":"Arredondo","year":"2017","journal-title":"Fla. Entomol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1603\/0022-0493-97.2.383","article-title":"Irradiation of Anastrepha obliqua (Diptera: Tephritidae) Revisited: Optimizing Sterility Induction","volume":"97","author":"Toledo","year":"2004","journal-title":"J. Econ. Entomol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1603\/0022-0493(2007)100[1153:IOALDT]2.0.CO;2","article-title":"Irradiation of Anastrepha ludens (Diptera: Tephritidae) Revisited: Optimizing Sterility Induction","volume":"100","author":"Rull","year":"2007","journal-title":"J. Econ. Entomol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1590\/1678-992x-2017-0253","article-title":"Quality control and characterization of the testicles and ovaries of irradiated Anastrepha obliqua from Brazil","volume":"77","author":"Costa","year":"2020","journal-title":"Sci. Agr."},{"key":"ref_59","unstructured":"Toledo-Arreola, J. (1992). Dosis \u00d3ptima de Irradiaci\u00f3n a Pupas de Anastrepha serpentina (Wiedemann) (Diptera: Tephritidae) Para la Obtenci\u00f3n de Adultos Est\u00e9riles Sexualmente Competitivos. [Master\u2019s Thesis, Instituto Tecnol\u00f3gico y de Estudios Superiores de Monterrey]."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.jinsphys.2015.11.011","article-title":"Male irradiation affects female remating behavior in Anastrepha serpentina (Diptera: Tephritidae)","volume":"85","author":"Arredondo","year":"2016","journal-title":"J. Insect Physiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1093\/jee\/toy013","article-title":"Packing and Postirradiation Handling of the Anastrepha ludens (Diptera: Tephritidae) Tapachula-7 Genetic Sexing StraIn Combined Effects of Hypoxia, Pupal Size, and Temperature on Adult Quality","volume":"111","author":"Arredondo","year":"2018","journal-title":"J. Econ. Entomol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1007\/s10340-020-01269-9","article-title":"Reduced quality of sterile Queensland fruit fly following post-production stress from hypoxia, irradiation and vibration","volume":"94","author":"Benelli","year":"2020","journal-title":"J. Pest Sci."}],"container-title":["Insects"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4450\/12\/4\/308\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:55:44Z","timestamp":1760363744000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4450\/12\/4\/308"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,30]]},"references-count":62,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2021,4]]}},"alternative-id":["insects12040308"],"URL":"https:\/\/doi.org\/10.3390\/insects12040308","relation":{},"ISSN":["2075-4450"],"issn-type":[{"value":"2075-4450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,30]]}}}