{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T17:59:12Z","timestamp":1780077552111,"version":"3.54.0"},"reference-count":65,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,1,12]],"date-time":"2022-01-12T00:00:00Z","timestamp":1641945600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["427879779"],"award-info":[{"award-number":["427879779"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["DR416\/12-1"],"award-info":[{"award-number":["DR416\/12-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Most of our knowledge on insect cuticular hydrocarbons (CHCs) stems from analytical techniques based on gas-chromatography coupled with mass spectrometry (GC-MS). However, this method has its limits under standard conditions, particularly in detecting compounds beyond a chain length of around C40. Here, we compare the CHC chain length range detectable by GC-MS with the range assessed by silver-assisted laser desorption\/ionization mass spectrometry (Ag-LDI-MS), a novel and rarely applied technique on insect CHCs, in seven species of the order Blattodea. For all tested species, we unveiled a considerable range of very long-chain CHCs up to C58, which are not detectable by standard GC-MS technology. This indicates that general studies on insect CHCs may frequently miss compounds in this range, and we encourage future studies to implement analytical techniques extending the conventionally accessed chain length range. Furthermore, we incorporate 3D scanned insect body surface areas as an additional factor for the comparative quantification of extracted CHC amounts between our study species. CHC quantity distributions differed considerably when adjusted for body surface areas as opposed to directly assessing extracted CHC amounts, suggesting that a more accurate evaluation of relative CHC quantities can be achieved by taking body surface areas into account.<\/jats:p>","DOI":"10.3390\/insects13010083","type":"journal-article","created":{"date-parts":[[2022,1,12]],"date-time":"2022-01-12T23:17:07Z","timestamp":1642029427000},"page":"83","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Neglected Very Long-Chain Hydrocarbons and the Incorporation of Body Surface Area Metrics Reveal Novel Perspectives for Cuticular Profile Analysis in Insects"],"prefix":"10.3390","volume":"13","author":[{"given":"Marek","family":"Golian","sequence":"first","affiliation":[{"name":"Institute for Evolution & Biodiversity, University of M\u00fcnster, H\u00fcfferstr. 1, D-48149 M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6080-548X","authenticated-orcid":false,"given":"Tanja","family":"Bien","sequence":"additional","affiliation":[{"name":"Institute of Hygiene, University of M\u00fcnster, Robert-Koch-Str. 41, D-48149 M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6707-6331","authenticated-orcid":false,"given":"Sebastian","family":"Schmelzle","sequence":"additional","affiliation":[{"name":"Ecological Networks, Technical University of Darmstadt, Schnittspahnstr. 2, D-64287 Darmstadt, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margy Alejandra","family":"Esparza-Mora","sequence":"additional","affiliation":[{"name":"Institute of Biology\u2014Zoology, Free University of Berlin, Unter den Eichen 87, D-12205 Berlin, Germany"},{"name":"Department for Materials and Environment, BAM Federal Institute for Materials Research and Testing, Unter den Eichen 87, D-12205 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dino Peter","family":"McMahon","sequence":"additional","affiliation":[{"name":"Institute of Biology\u2014Zoology, Free University of Berlin, Unter den Eichen 87, D-12205 Berlin, Germany"},{"name":"Department for Materials and Environment, BAM Federal Institute for Materials Research and Testing, Unter den Eichen 87, D-12205 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Dreisewerd","sequence":"additional","affiliation":[{"name":"Institute of Hygiene, University of M\u00fcnster, Robert-Koch-Str. 41, D-48149 M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8493-692X","authenticated-orcid":false,"given":"Jan","family":"Buellesbach","sequence":"additional","affiliation":[{"name":"Institute for Evolution & Biodiversity, University of M\u00fcnster, H\u00fcfferstr. 1, D-48149 M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,12]]},"reference":[{"key":"ref_1","first-page":"269","article-title":"The physiology of the cuticle and of ecdysis in Rhodnius prolixus (Triatomidae, Hemiptera); with special reference to the function of the oenocytes and of the dermal glands","volume":"76","author":"Wigglesworth","year":"1933","journal-title":"Q. 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