{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T06:08:53Z","timestamp":1789538933537,"version":"build-2803163510"},"reference-count":54,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,3,20]],"date-time":"2022-03-20T00:00:00Z","timestamp":1647734400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"OARDC SEEDS Partnership Grant","award":["2019009"],"award-info":[{"award-number":["2019009"]}]},{"name":"Vaylenx, LLC","award":["2019009"],"award-info":[{"award-number":["2019009"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>The yellow fever mosquito Aedes aegypti is one of the deadliest animals on the planet because it transmits several medically important arboviruses, including Zika, chikungunya, dengue, and yellow fever. Carbon-based nanoparticles (CNPs) derived from natural sources have previously been shown to have toxic effects on mosquito larvae and offer a potential alternative to chemical insecticides such as pyrethroids, for which mosquitoes have evolved resistance. However, CNPs derived from industrial sources, such as carbon black, have not previously been evaluated as larvicides. Here, we evaluate the effects of a commercially-available carbon black, EMPEROR\u00ae 1800 (E1800), on mortality and development of pyrethroid-susceptible (PS) and pyrethroid-resistant (PR) strains of Ae. aegypti. We found that E1800 exhibited concentration-dependent mortality against 1st instar larvae of both strains within the first 120 h after exposure, but after this period, surviving larvae did not show delays in their development to adults. Physical characterization of E1800 suspensions suggests that they form primary particles of ~30 nm in diameter that fuse into fundamental aggregates of ~170 nm in diameter. Notably, larvae treated with E1800 showed internal accumulation of E1800 in the gut and external accumulation on the respiratory siphon, anal papillae, and setae, suggesting a physical mode of toxic action. Taken together, our results suggest that E1800 has potential use as a larvicide with a novel mode of action for controlling PS and PR mosquitoes.<\/jats:p>","DOI":"10.3390\/insects13030307","type":"journal-article","created":{"date-parts":[[2022,3,20]],"date-time":"2022-03-20T21:30:14Z","timestamp":1647811814000},"page":"307","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Larvicidal Activity of Carbon Black against the Yellow Fever Mosquito Aedes aegypti"],"prefix":"10.3390","volume":"13","author":[{"given":"Erick J.","family":"Mart\u00ednez Rodr\u00edguez","sequence":"first","affiliation":[{"name":"Ohio Agricultural Research and Development Center, Department of Entomology, The Ohio State University, Wooster, OH 44691, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parker","family":"Evans","sequence":"additional","affiliation":[{"name":"Indiana Center for Regenerative Medicine & Engineering, Indiana University Health Comprehensive Wound Center, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megha","family":"Kalsi","sequence":"additional","affiliation":[{"name":"Lepidext Inc., Lexington, KY 40505, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noah","family":"Rosenblatt","sequence":"additional","affiliation":[{"name":"Vaylenx, LLC, Washington, DC 20003, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morgan","family":"Stanley","sequence":"additional","affiliation":[{"name":"Vaylenx, LLC, Washington, DC 20003, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1020-5774","authenticated-orcid":false,"given":"Peter M.","family":"Piermarini","sequence":"additional","affiliation":[{"name":"Ohio Agricultural Research and Development Center, Department of Entomology, The Ohio State University, Wooster, OH 44691, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1086\/511869","article-title":"Yellow Fever: Epidemiology and Prevention","volume":"44","author":"Barnett","year":"2007","journal-title":"Clin. 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