{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T19:47:31Z","timestamp":1785527251620,"version":"3.56.0"},"reference-count":30,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,6,28]],"date-time":"2022-06-28T00:00:00Z","timestamp":1656374400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31750002"],"award-info":[{"award-number":["31750002"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32070475"],"award-info":[{"award-number":["32070475"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)","award":["31750002"],"award-info":[{"award-number":["31750002"]}]},{"name":"Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)","award":["32070475"],"award-info":[{"award-number":["32070475"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>The monotypic family Deuterophlebiidae of China was recorded twice previously from far northwest upon adults, the most parts of this country have not been investigated, leaving a huge blank of knowledge on their morphology, diversity, biology, or distribution. After deliberated collecting and rearing in recent years, we obtained more than one thousand specimens of Deuterophlebiidae, they are classified into five new species herein: Deuterophlebia sinensis sp. nov., D. yunnanensis sp. nov., D. wuyiensis sp. nov., D. acutirhina sp. nov. and D. alata sp. nov. Detailed descriptions and photographs of gathered life stages are given for these new species. Adults of them can be identified by chaetotaxy and length ratio of flagellomeres and legs, microtrichia on postgena and shape of their clypeus, pupae can be recognized by thoracic spines and abdominal chitin bands, and larvae can be separated by setae on thorax and abdomen. Genetic distances between species are 0.086\u20130.175 based on their COI genes. This contribution represents the first database of the enigmatic Deuterophlebiidae from China and shows a new distribution pattern of Deuterophlebia. In addition, the discovery throws some light on the origin and biogeography of the genus and family.<\/jats:p>","DOI":"10.3390\/insects13070593","type":"journal-article","created":{"date-parts":[[2022,6,28]],"date-time":"2022-06-28T23:59:22Z","timestamp":1656460762000},"page":"593","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Descriptions and Barcoding of Five New Chinese Deuterophlebia Species Revealing This Genus in Both Holarctic and Oriental Realms (Diptera: Deuterophlebiidae)"],"prefix":"10.3390","volume":"13","author":[{"given":"Xuhongyi","family":"Zheng","sequence":"first","affiliation":[{"name":"The Key Laboratory of Jiangsu Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiteng","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengxu","family":"Mu","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Jiangsu Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2100-6140","authenticated-orcid":false,"given":"Zhenxing","family":"Ma","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Jiangsu Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changfa","family":"Zhou","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Jiangsu Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,28]]},"reference":[{"key":"ref_1","unstructured":"Courtney, G.W. (1989). Morphology, Systematics and Ecology of Mountain Midges (Diptera: Deuterophlebiidae). [Ph.D. Thesis, University of Alberta]."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1080\/00222939000770071","article-title":"Revision of Nearctic m.ountain midges (Diptera: Deuterophlebiidae)","volume":"24","author":"Courtney","year":"1990","journal-title":"J. Nat. Hist."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1365-3113.1994.tb00576.x","article-title":"Revision of Palaearctic mountain midges (Diptera: Deuterophlebiidae), with phylogenetic and biogeographic analyses of world species","volume":"19","author":"Courtney","year":"1994","journal-title":"Syst. Entomol."},{"key":"ref_4","unstructured":"Yule, C.M., and Sen, Y.H. (2004). Insecta: Diptera, Deuterophlebiidae, Freshwater Invertebrates of the Malaysian Region."},{"key":"ref_5","first-page":"94","article-title":"Deuterophlebia Edwards, 1922 (Diptera: Deuterophlebiidae) an Enigmatic Primitive Diptera (Insecta) from Kashmir Himalaya","volume":"11","author":"Sofi","year":"2020","journal-title":"Halteres"},{"key":"ref_6","first-page":"973","article-title":"Deuterophlebia sajanica sp. n., a new species of mountain midges from Mongolia (Diptera: Deuterophlebiidae)","volume":"36","author":"Jedlicka","year":"1981","journal-title":"Biologia"},{"key":"ref_7","first-page":"487","article-title":"The Deuterophlebiidae of Japan","volume":"17","author":"Kitakami","year":"1938","journal-title":"Annot. Zool. Jpn."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"201","DOI":"10.2307\/3224115","article-title":"Biology and life history of a new species of mountain midge, Deuterophlebia nielsoni, from eastern California (Diptera: Deuterophlebiidae)","volume":"77","author":"Kennedy","year":"1958","journal-title":"Trans. Am. Microsc. Soc."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"191","DOI":"10.2307\/3224086","article-title":"Deuterophlebia inyoensis, a new species of mountain midge from the alpine zone of the Sierra Nevada Range, California (Diptera: Deuterophlebiidae)","volume":"79","author":"Kennedy","year":"1960","journal-title":"Trans. Am. Microsc. Soc."},{"key":"ref_10","first-page":"1","article-title":"Notes on mountain midges (Deuterophlebiidae) with a description of the immature stages of a new species from Colorado","volume":"1276","author":"Pennak","year":"1945","journal-title":"Am. Mus. Novit."},{"key":"ref_11","unstructured":"Pennak, R.W. (2022, June 05). Description of the imago of the mountain midge Deuterophlebia coloradensis Pennak (Diptera, Deuterophlebiidae). Am. Mus. Novit., Available online: https:\/\/digitallibrary.amnh.org\/bitstream\/handle\/2246\/2378\/N1534.pdf?sequence=1."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"204","DOI":"10.4039\/Ent86204-5","article-title":"First record of the family Deuterophlebiidae in Canada (Diptera)","volume":"86","author":"Shewell","year":"1954","journal-title":"Can. Entomol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1007\/BF00419212","article-title":"Zur Kenntniss der Wirbellosenfauna der Bergstr\u00f6me Mittelasiens. II. Deuterophlebia mirabilis EDW","volume":"18","author":"Brodsky","year":"1930","journal-title":"Z. Morphol. Okol. Tiere"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1080\/00222932208632687","article-title":"XLVI.-Deuterophlebia mirabilis, gen. et sp. n., a remarkable Dipterous insect from Kashmir","volume":"9","author":"Edwards","year":"1922","journal-title":"Ann. Mag. Nat. Hist."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1111\/j.1365-2311.1924.tb03350.x","article-title":"Metamorphosis of Deuterophlebia sp. (Diptera: Deuterophlebiidae Edw.)","volume":"1924","author":"Pulikovsky","year":"1924","journal-title":"Trans. Entomol. Soc. Lond."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1139\/z90-081","article-title":"Cuticular morphology of larval mountain midges (Diptera: Deuterophlebiidae): Implications for the phylogenetic relationships of Nematocera","volume":"68","author":"Courtney","year":"1990","journal-title":"Can. J. Zool."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1111\/j.1469-7998.1962.tb05689.x","article-title":"The structure and function of the spiracular gills of Deuterophlebia (Deuterophlebiidae) in relation to those of other Diptera","volume":"138","author":"Hinton","year":"1962","journal-title":"Proc. Zool. Soc. Lond."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.asd.2010.07.002","article-title":"Adult head structures of Deuterophlebiidae (Insecta), a highly derived \u201cancestral\u201d dipteran lineage","volume":"40","author":"Schneeberg","year":"2011","journal-title":"Arthropod Struct. Dev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1111\/j.1365-3113.1991.tb00683.x","article-title":"Phylogenetic analysis of the Blephariceromorpha, with special reference to mountain midges (Diptera: Deuterophlebiidae)","volume":"16","author":"Courtney","year":"1991","journal-title":"Syst. Entomol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1111\/j.1365-3113.2008.00437.x","article-title":"Phylogenetics and temporal diversification of the earliest true flies (Insecta: Diptera) based on multiple nuclear genes","volume":"33","author":"Bertone","year":"2008","journal-title":"Syst. Entomol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1146\/annurev.ento.44.1.397","article-title":"Congruence and controversy: Toward a higher-level phylogeny of Diptera","volume":"44","author":"Yeates","year":"1999","journal-title":"Annu. Rev. Entomol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1111\/j.1365-3113.2006.00355.x","article-title":"A mitochondrial genome phylogeny of Diptera: Whole genome sequence data accurately resolve relationships over broad timescales with high precision","volume":"32","author":"Cameron","year":"2007","journal-title":"Syst. Entomol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"565","DOI":"10.11646\/zootaxa.1668.1.27","article-title":"Phylogeny and systematics of Diptera: Two decades of progress and prospects","volume":"1668","author":"Yeates","year":"2007","journal-title":"Zootaxa"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1006\/zjls.1995.0080","article-title":"Phylogeny of the nematocerous families of Diptera (Insecta)","volume":"115","author":"Oosterbroek","year":"1995","journal-title":"J. Linn. Soc. Lond. Zool."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Zhang, X., Yang, D., and Kang, Z. (2022). New data on the mitochondrial genome of Nematocera (lower Diptera): Features, structures and phylogenetic implications. J. Linn. Soc. Lond. Zool., 1\u201317.","DOI":"10.1093\/zoolinnean\/zlac012"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1080\/01650424.2020.1871026","article-title":"First detailed description of adults and nymph of Cincticostella femorata (Tshernova, 1972) (Ephemeroptera: Ephemerellidae)","volume":"42","author":"Zheng","year":"2021","journal-title":"Aquat. Insects"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1163\/1876312X-bja10028","article-title":"A new subgenus of Epeorus and its five species from China (Ephemeroptera: Heptageniidae)","volume":"53","author":"Ma","year":"2022","journal-title":"Insect Syst. Evol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"353","DOI":"10.11646\/zootaxa.4341.3.2","article-title":"Mayflies of the genus Epeorus Eaton, 1881 s. l. (Ephemeroptera: Heptageniidae) from the Caucasus Mountains: A new species of Caucasiron Kluge, 1997 from Georgia and Turkey","volume":"4341","author":"Hrivniak","year":"2017","journal-title":"Zootaxa"},{"key":"ref_29","first-page":"294","article-title":"DNA primers for amplification of mitochondrial cytochrome C oxidase subunit I from diverse metazoan invertebrates","volume":"3","author":"Folmer","year":"1994","journal-title":"Mol. Mar. Biol. Biotechnol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"177","DOI":"10.2307\/1467577","article-title":"Life history patterns of Nearctic mountain midges (Diptera: Deuterophlebiidae)","volume":"10","author":"Courtney","year":"1991","journal-title":"J. N. Am. Benthol. Soc."}],"container-title":["Insects"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4450\/13\/7\/593\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:39:56Z","timestamp":1760139596000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4450\/13\/7\/593"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,28]]},"references-count":30,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2022,7]]}},"alternative-id":["insects13070593"],"URL":"https:\/\/doi.org\/10.3390\/insects13070593","relation":{},"ISSN":["2075-4450"],"issn-type":[{"value":"2075-4450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,28]]}}}