{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T07:28:35Z","timestamp":1791530915413,"version":"4.3.4"},"reference-count":60,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,10,2]],"date-time":"2022-10-02T00:00:00Z","timestamp":1664668800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the International Atomic Energy Agency, Vienna","award":["RC-20565\/RB"],"award-info":[{"award-number":["RC-20565\/RB"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Radiobiological investigations on the reproductive behavior of female Spodoptera litura (Fabr.) were conducted with the aim of determining the suitable radio-sterilizing dose for females in order to release them along with sub-sterile males for effective implementation of the Inherited Sterility technique against this pest. Calling and copulation duration significantly increased, while mating success, oviposition, fertility and longevity significantly decreased with increasing radiation dose (100\u2013200 Gy) compared to control. In view of the effect of irradiation on mating behavior and reproductive viability of female S. litura, 130 Gy was identified as a suitable radio-sterilization dose. Further molecular studies were conducted to corroborate this dose for female sterilization, along with a higher dose of 200 Gy in order to validate the gradational response of ionizing radiation. GC-MS analysis indicated decreased sex pheromone titer at 130 Gy, which was more pronounced at 200 Gy. Pheromone-associated genes, PBAN and PBAN-R showed decreased expression at 130 Gy, and were drastically reduced at 200 Gy. The fertility-related Vg gene also showed a negative correlation with radiation exposure. Based on these radiation responses of female S. litura, 130 Gy might be considered a suitable dose for complete female sterility and its inclusion in sterile insect programs against S. litura.<\/jats:p>","DOI":"10.3390\/insects13100898","type":"journal-article","created":{"date-parts":[[2022,10,8]],"date-time":"2022-10-08T23:39:31Z","timestamp":1665272371000},"page":"898","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Effect of Irradiation on Reproduction of Female Spodoptera litura (Fabr.) (Lepidoptera: Noctuidae) in Relation to the Inherited Sterility Technique"],"prefix":"10.3390","volume":"13","author":[{"given":"Madhumita","family":"Sengupta","sequence":"first","affiliation":[{"name":"Applied Entomology and Radiation Biology Lab, Department of Zoology, University of Delhi, Delhi 110007, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neha","family":"Vimal","sequence":"additional","affiliation":[{"name":"Applied Entomology and Radiation Biology Lab, Department of Zoology, University of Delhi, Delhi 110007, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nilza","family":"Angmo","sequence":"additional","affiliation":[{"name":"Applied Entomology and Radiation Biology Lab, Department of Zoology, University of Delhi, Delhi 110007, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rakesh Kumar","family":"Seth","sequence":"additional","affiliation":[{"name":"Applied Entomology and Radiation Biology Lab, Department of Zoology, University of Delhi, Delhi 110007, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"35","DOI":"10.2307\/1313226","article-title":"Evolution of feeding behaviour in insect herbivores","volume":"48","author":"Bernays","year":"1998","journal-title":"Bioscience"},{"key":"ref_2","first-page":"e05765","article-title":"EFSA Panel on Plant Health (PLH) Pest categorisation of Spodoptera litura","volume":"17","author":"Bragard","year":"2019","journal-title":"EFSA J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1002\/(SICI)1096-9063(199707)50:3<240::AID-PS579>3.0.CO;2-9","article-title":"Status of insecticide resistance in Spodoptera litura in Andhra Pradesh, India","volume":"50","author":"Armes","year":"1997","journal-title":"Pestic. 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