{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T23:07:21Z","timestamp":1780614441122,"version":"3.54.1"},"reference-count":43,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,11,16]],"date-time":"2022-11-16T00:00:00Z","timestamp":1668556800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Institut Pasteur de Tunis","award":["R21AI117107-01A1, EZ"],"award-info":[{"award-number":["R21AI117107-01A1, EZ"]}]},{"DOI":"10.13039\/100000002","name":"US National Institutes of Health","doi-asserted-by":"publisher","award":["R21AI117107-01A1, EZ"],"award-info":[{"award-number":["R21AI117107-01A1, EZ"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Based on nucleotide sequences, we re-identified representative samples of Phlebotomus perfiliewi originating from two different biogeographical areas of Tunisia, whose populations had previously been identified based on morphological criteria. A partial region of the mitochondrial DNA cytochrome b gene was targeted, and sandfly species was determined by analogy with DNA sequences available in the GenBank database via a BLAST analysis, taking into account the query coverage and percentage identity. The recognized species presents the most substantial homology with the analyzed sequence. The results of the molecular identification showed complete agreement with the morphological identifications, and Phlebotomus perfiliewi is genetically variable.<\/jats:p>","DOI":"10.3390\/insects13111057","type":"journal-article","created":{"date-parts":[[2022,11,16]],"date-time":"2022-11-16T02:24:53Z","timestamp":1668565493000},"page":"1057","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Investigation of the Sandfly Fauna of Central Arid Areas and Northern Humid Regions of Tunisia, with Morphological and Molecular Identification of the Recently Established Population of Phlebotomus (Larroussius) perfiliewi"],"prefix":"10.3390","volume":"13","author":[{"given":"Ifhem","family":"Chelbi","sequence":"first","affiliation":[{"name":"Unit of Vector Ecology, Pasteur Institute of Tunis, 13 Place Pasteur BP 74, 1002 Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amani","family":"Abdi","sequence":"additional","affiliation":[{"name":"Unit of Vector Ecology, Pasteur Institute of Tunis, 13 Place Pasteur BP 74, 1002 Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6995-0088","authenticated-orcid":false,"given":"J\u00e9r\u00f4me","family":"Depaquit","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Reims Champagne-Ardenne, ANSES, SFR Cap Sant\u00e9, EA4688 ESCAPE\u2013USC \u00abTransmission Vectorielle et \u00c9pid\u00e9miosurveillance de Maladies Parasitaires (VECPAR)\u00bb, 51 rue Cognacq-Jay, CEDEX, 51096 Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0452-2856","authenticated-orcid":false,"given":"Wasfi","family":"Fares","sequence":"additional","affiliation":[{"name":"Unit of Vector Ecology, Pasteur Institute of Tunis, 13 Place Pasteur BP 74, 1002 Tunis, Tunisia"},{"name":"Laboratory of Clinical Virology, Pasteur Institute of Tunis, 13 Place Pasteur BP 74, 1002 Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9428-2461","authenticated-orcid":false,"given":"Mohammed Abdo Saghir","family":"Abbas","sequence":"additional","affiliation":[{"name":"Unit of Vector Ecology, Pasteur Institute of Tunis, 13 Place Pasteur BP 74, 1002 Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khalil","family":"Dachraoui","sequence":"additional","affiliation":[{"name":"Unit of Vector Ecology, Pasteur Institute of Tunis, 13 Place Pasteur BP 74, 1002 Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9188-6227","authenticated-orcid":false,"given":"Elyes","family":"Zhioua","sequence":"additional","affiliation":[{"name":"Unit of Vector Ecology, Pasteur Institute of Tunis, 13 Place Pasteur BP 74, 1002 Tunis, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/0035-9203(93)90011-E","article-title":"Phlebotomus (Larroussius) perfiliewi naturally infected with dermotropic Leishmania infantum at Tenes, Algeria","volume":"87","author":"Izri","year":"1993","journal-title":"Trans. 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Pathogens, 11.","DOI":"10.3390\/pathogens11080855"}],"container-title":["Insects"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4450\/13\/11\/1057\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:19:03Z","timestamp":1760145543000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4450\/13\/11\/1057"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,16]]},"references-count":43,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2022,11]]}},"alternative-id":["insects13111057"],"URL":"https:\/\/doi.org\/10.3390\/insects13111057","relation":{},"ISSN":["2075-4450"],"issn-type":[{"value":"2075-4450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,16]]}}}