{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T07:08:25Z","timestamp":1781680105153,"version":"3.54.5"},"reference-count":66,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T00:00:00Z","timestamp":1678320000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI148551"],"award-info":[{"award-number":["R01AI148551"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>The mosquito midgut is an important site for bloodmeal regulation while also acting as a primary site for pathogen exposure within the mosquito. Recent studies show that exposure to dehydrating conditions alters mosquito bloodfeeding behaviors as well as post-feeding regulation, likely altering how pathogens interact with the mosquito. Unfortunately, few studies have explored the underlying dynamics between dehydration and bloodmeal utilization, and the overall impact on disease transmission dynamics remains veiled. In this study, we find that dehydration-based feeding in the yellow fever mosquito, Aedes aegypti, prompts alterations to midgut gene expression, as well as subsequent physiological factors involving water control and post-bloodfeeding (pbf) regulation. Altered expression of ion transporter genes and aquaporin 2 (AQP2) in the midgut of dehydrated mosquitoes as well as the rapid reequilibration of hemolymph osmolality after a bloodmeal indicate an ability to expedite fluid and ion processing. These alterations ultimately indicate that female A. aegypti employ mechanisms to ameliorate the detriments of dehydration by imbibing a bloodmeal, providing an effective avenue for rehydration. Continued research into bloodmeal utilization and the resulting effects on arthropod-borne transmission dynamics becomes increasingly important as drought prevalence is increased by climate change.<\/jats:p>","DOI":"10.3390\/insects14030274","type":"journal-article","created":{"date-parts":[[2023,3,10]],"date-time":"2023-03-10T02:05:54Z","timestamp":1678413954000},"page":"274","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Dehydration Alters Transcript Levels in the Mosquito Midgut, Likely Facilitating Rapid Rehydration following a Bloodmeal"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9612-366X","authenticated-orcid":false,"given":"Christopher J.","family":"Holmes","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, University of Cincinnati, Cincinnati, OH 45221, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elliott S.","family":"Brown","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Cincinnati, Cincinnati, OH 45221, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhriti","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Cincinnati, Cincinnati, OH 45221, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9993-7976","authenticated-orcid":false,"given":"Matthew","family":"Warden","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Cincinnati, Cincinnati, OH 45221, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atit","family":"Pathak","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Cincinnati, Cincinnati, OH 45221, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blaine","family":"Payton","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Cincinnati, Cincinnati, OH 45221, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quynh","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Cincinnati, Cincinnati, OH 45221, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Austin","family":"Spangler","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Cincinnati, Cincinnati, OH 45221, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaishna","family":"Sivakumar","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Cincinnati, Cincinnati, OH 45221, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob M.","family":"Hendershot","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Cincinnati, Cincinnati, OH 45221, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua B.","family":"Benoit","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Cincinnati, Cincinnati, OH 45221, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"6804","DOI":"10.1038\/s41598-018-24893-z","article-title":"Dehydration Prompts Increased Activity and Blood Feeding by Mosquitoes","volume":"8","author":"Hagan","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1016\/S0022-1910(99)00085-2","article-title":"Adaptation of Aedes aegypti (Diptera: Culicidae) Oviposition Behavior in Response to Humidity and Diet","volume":"45","author":"Canyon","year":"1999","journal-title":"J. Insect Physiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1590\/S0085-56262010000300021","article-title":"Impact of Small Variations in Temperature and Humidity on the Reproductive Activity and Survival of Aedes aegypti (Diptera, Culicidae)","volume":"54","author":"Costa","year":"2010","journal-title":"Rev. Bras. Entomol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1093\/jee\/64.2.442","article-title":"A Study of the Probing Response of Aedes aegypti. 4. Effect of Dry and Moist Heat on Probing","volume":"64","author":"Khan","year":"1971","journal-title":"J. Econ. Entomol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1016\/0022-1910(68)90018-8","article-title":"The Effect of Temperature and Relative Humidity on the Flight Performance of Female Aedes aegypti","volume":"14","author":"Rowley","year":"1968","journal-title":"J. Insect Physiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1017\/S0007485300030698","article-title":"The Effect of a Difference in Temperature and Humidity on Certain Reactions of Female Aedes aegypti (L.)","volume":"43","author":"Parker","year":"1952","journal-title":"Bull. Entomol. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"104363","DOI":"10.1016\/j.jinsphys.2022.104363","article-title":"Bloodmeal Regulation in Mosquitoes Curtails Dehydration-Induced Mortality, Altering Vectorial Capacity","volume":"137","author":"Holmes","year":"2022","journal-title":"J. Insect Physiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2763","DOI":"10.1242\/jeb.044883","article-title":"Repeated Bouts of Dehydration Deplete Nutrient Reserves and Reduce Egg Production in the Mosquito Culex pipiens","volume":"213","author":"Benoit","year":"2010","journal-title":"J. Exp. Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1186\/1756-3305-7-176","article-title":"Cuticular Differences Associated with Aridity Acclimation in African Malaria Vectors Carrying Alternative Arrangements of Inversion 2La","volume":"7","author":"Reidenbach","year":"2014","journal-title":"Parasites Vectors"},{"key":"ref_10","first-page":"1","article-title":"Aedes aegypti Disregard Humidity-Related Conditions with Adequate Nutrition","volume":"30","author":"Canyon","year":"2013","journal-title":"Trop. Biomed."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1093\/aesa\/63.4.995","article-title":"Day-to-Day Change in Relative Humidity and the Activity of Culex nigripalpus (Diptera: Culicidae)","volume":"63","author":"Dow","year":"1970","journal-title":"Ann. Entomol. Soc. Am."},{"key":"ref_12","first-page":"3823","article-title":"Desiccation Tolerance as a Function of Age, Sex, Humidity and Temperature in Adults of the African Malaria Vectors Anopheles arabiensis and Anopheles funestus","volume":"217","author":"Lyons","year":"2014","journal-title":"J. Exp. Biol."},{"key":"ref_13","first-page":"103","article-title":"Effect of Temperature and Humidity on Life Cycle Duration of Culex quinquefasciatus Say (Diptera : Culicidae) at Muzaffarpur (Bihar), India","volume":"6","author":"Kumar","year":"2015","journal-title":"Adv. Biores."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1017\/S0007485300004612","article-title":"Longevity of Anopheles maculipennis Race Atroparvus, van Thiel, at Controlled Temperature and Humidity after One Blood Meal","volume":"30","author":"Leeson","year":"1939","journal-title":"Bull. Entomol. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"986","DOI":"10.2307\/4577885","article-title":"Notes on the Influence of Temperature and Humidity on Oviposition and Early Life of Anopheles","volume":"41","author":"Mayne","year":"1926","journal-title":"Public Health Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1756-3305-3-19","article-title":"Local Impact of Temperature and Precipitation on West Nile Virus Infection in Culex Species Mosquitoes in Northeast Illinois, USA","volume":"3","author":"Ruiz","year":"2010","journal-title":"Parasites Vectors"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Holmes, C.J., and Benoit, J.B. (2019). Biological Adaptations Associated with Dehydration in Mosquitoes. Insects, 10.","DOI":"10.3390\/insects10110375"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1089\/vbz.2007.0189","article-title":"Nutritional Stress Affects Mosquito Survival and Vector Competence for West Nile Virus","volume":"8","author":"Vaidyanathan","year":"2008","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1093\/jmedent\/30.6.975","article-title":"Multiple Blood Meals as a Reproductive Strategy in Anopheles (Diptera: Culicidae)","volume":"30","author":"Briegel","year":"1993","journal-title":"J. Med. Entomol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1111\/j.1365-3032.1978.tb00164.x","article-title":"Arrest, Resorption, or Maturation of Oocytes in Aedes aegypti: Dependence on the Quantity of Blood and the Interval between Blood Meals","volume":"3","author":"Lea","year":"1978","journal-title":"Physiol. Entomol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"20130561","DOI":"10.1098\/rstb.2013.0561","article-title":"Climate Change Impacts on West Nile Virus Transmission in a Global Context","volume":"370","author":"Paz","year":"2015","journal-title":"Philos. Trans. R. Soc. B Biol. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e3096","DOI":"10.7717\/peerj.3096","article-title":"Vector Competence of Anopheles and Culex Mosquitoes for Zika Virus","volume":"5","author":"Dodson","year":"2017","journal-title":"PeerJ"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1089\/vbz.2016.1940","article-title":"Detection of Sindbis and Inkoo Virus RNA in Genetically Typed Mosquito Larvae Sampled in Northern Sweden","volume":"16","author":"Spyckerelle","year":"2016","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"17504","DOI":"10.1073\/pnas.1316709110","article-title":"Impact of Trehalose Transporter Knockdown on Anopheles gambiae Stress Adaptation and Susceptibility to Plasmodium falciparum Infection","volume":"110","author":"Liu","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1093\/jmedent\/36.1.13","article-title":"Utilization of Human Blood and Sugar as Nutrients by Female Aedes aegypti (Diptera: Culicidae)","volume":"36","author":"Naksathit","year":"1999","journal-title":"J. Med. Entomol"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e15578","DOI":"10.1371\/journal.pone.0015578","article-title":"The Aquaporin Gene Family of the Yellow Fever Mosquito, Aedes aegypti","volume":"5","author":"Drake","year":"2010","journal-title":"PLoS ONE"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1007\/BF00689629","article-title":"Dynamic Changes in Flow Rate and Composition of Urine during the Post-Bloodmeal Diuresis in Aedes aegypti (L.)","volume":"153","author":"Williams","year":"1983","journal-title":"J. Comp. Physiol. B"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1038\/s41586-018-0692-z","article-title":"Improved Reference Genome of Aedes aegypti Informs Arbovirus Vector Control","volume":"563","author":"Matthews","year":"2018","journal-title":"Nature"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"W537","DOI":"10.1093\/nar\/gky379","article-title":"The Galaxy Platform for Accessible, Reproducible and Collaborative Biomedical Analyses: 2018 Update","volume":"46","author":"Afgan","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_30","unstructured":"Andrews, S. (2023, February 26). FastQC: A Quality Control Tool for High Throughput. Available online: http:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A Flexible Trimmer for Illumina Sequence Data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1038\/nbt.3519","article-title":"Near-Optimal Probabilistic RNA-Seq Quantification","volume":"34","author":"Bray","year":"2016","journal-title":"Nat. Biotechnol."},{"key":"ref_33","first-page":"462","article-title":"Sailfish enables alignment-free isoform quantification from RNA-seq reads using lightweight algorithms Nat","volume":"32","author":"Patro","year":"2014","journal-title":"Biotechnol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated Estimation of Fold Change and Dispersion for RNA-Seq Data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1093\/nar\/gkm226","article-title":"G:Profiler-a Web-Based Toolset for Functional Profiling of Gene Lists from Large-Scale Experiments","volume":"35","author":"Reimand","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Supek, F., Bo\u0161njak, M., \u0160kunca, N., and \u0160muc, T. (2011). REVIGO Summarizes and Visualizes Long Lists of Gene Ontology Terms. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0021800"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12859-019-2671-2","article-title":"CirGO: An Alternative Circular Way of Visualising Gene Ontology Terms","volume":"20","author":"Kuznetsova","year":"2019","journal-title":"BMC Bioinform."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"R106","DOI":"10.1186\/gb-2010-11-10-r106","article-title":"Differential Expression Analysis for Sequence Count Data","volume":"11","author":"Anders","year":"2010","journal-title":"Genome Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2008","DOI":"10.1101\/gr.133744.111","article-title":"Detecting Differential Usage of Exons from RNA-Seq Data","volume":"22","author":"Anders","year":"2012","journal-title":"Genome Res."},{"key":"ref_40","first-page":"1","article-title":"Age-Grouping Methods in Diptera of Medical Importance With Special Reference to Some Vectors of Malaria","volume":"47","author":"Detinova","year":"1962","journal-title":"World Health Organ. Monogr. Ser."},{"key":"ref_41","unstructured":"(2023, February 26). The GIMP Development Team GIMP. Available online: https:\/\/www.gimp.org."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/0022-1910(94)90044-2","article-title":"Redirection of Metabolism in the Flesh Fly, Sarcophaga bullata, Following Envenomation by the Ectoparasitoid Nasonia vitripennis and Correlation of Metabolic Effects with the Diapause Status of the Host","volume":"40","author":"Rivers","year":"1994","journal-title":"J. Insect Physiol."},{"key":"ref_43","first-page":"299","article-title":"Rapid Determination of Glycogen and Sugars in Mosquitoes","volume":"1","year":"1985","journal-title":"J. Am. Mosq. Control Assoc."},{"key":"ref_44","first-page":"302","article-title":"Rapid Determination of Total Lipids in Mosquitoes","volume":"1","year":"1985","journal-title":"J. Am. Mosq. Control Assoc."},{"key":"ref_45","unstructured":"(2023, February 26). Microsoft Corporation Microsoft Excel. Available online: https:\/\/office.microsoft.com\/excel."},{"key":"ref_46","unstructured":"R Core Team (2021). R: A Language and Environment for Statistical Computing, R Core Team."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1","DOI":"10.18637\/jss.v040.i01","article-title":"The Split-Apply-Combine Strategy for Data Analysis","volume":"40","author":"Wickham","year":"2011","journal-title":"J. Stat. Softw."},{"key":"ref_48","unstructured":"Wickham, H. (2023, February 26). Tidyr: Tidy Messy Data 2020. Available online: https:\/\/tidyr.tidyverse.org."},{"key":"ref_49","unstructured":"Wickham, H., Francois, R., Henry, L., and M\u00fcller, K. (2023, February 26). Dplyr: A Grammar of Data Manipulation 2017. Available online: https:\/\/dplyr.tidyverse.org."},{"key":"ref_50","unstructured":"Hope, R.M. (2023, February 26). Rmisc: Rmisc: Ryan Miscellaneous 2013. Available online: https:\/\/cran.r-project.org\/package=Rmisc."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Wickham, H. (2009). Ggplot2: Elegant Graphics for Data Analysis, Springer.","DOI":"10.1007\/978-0-387-98141-3"},{"key":"ref_52","unstructured":"Inkscape Project (2023, February 26). Inkscape 2020. Available online: https:\/\/inkscape.org."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"283","DOI":"10.3389\/fphys.2017.00283","article-title":"RNA-Seq Comparison of Larval and Adult Malpighian Tubules of the Yellow Fever Mosquito Aedes aegypti Reveals Life Stage-Specific Changes in Renal Function","volume":"8","author":"Li","year":"2017","journal-title":"Front. Physiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1093\/jmedent\/12.1.99","article-title":"The Effects of Nutrition on Survival and Fecundity on Florida Mosquitoes","volume":"12","author":"Nayar","year":"1975","journal-title":"J. Med. Ent"},{"key":"ref_55","first-page":"129","article-title":"The Genome Sequence of the Malaria Mosquito Anopheles gambiae","volume":"298","author":"Holt","year":"2002","journal-title":"October"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1146\/annurev.en.40.010195.002303","article-title":"Mosquito Sugar Feeding and Reproductive Energetics","volume":"40","author":"Foster","year":"1995","journal-title":"Annu. Rev. Entomol."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Lehane, M.J. (2005). The Biology of Blood-Sucking in Insects, Second Edition, Cambridge University Press.","DOI":"10.1017\/CBO9780511610493"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1016\/j.ibmb.2005.07.006","article-title":"Sindbis Virus Induces Transport Processes and Alters Expression of Innate Immunity Pathway Genes in the Midgut of the Disease Vector, Aedes aegypti","volume":"35","author":"Sanders","year":"2005","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"7795","DOI":"10.1038\/srep07795","article-title":"Functional Characterization of Aquaporins and Aquaglyceroporins of the Yellow Fever Mosquito, Aedes aegypti","volume":"5","author":"Drake","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"6062","DOI":"10.1073\/pnas.1102629108","article-title":"Aquaporin Water Channel AgAQP1 in the Malaria Vector Mosquito Anopheles gambiae during Blood Feeding and Humidity Adaptation","volume":"108","author":"Liu","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Tsujimoto, H., Liu, K., Linser, P.J., Agre, P., and Rasgon, J.L. (2013). Organ-Specific Splice Variants of Aquaporin Water Channel AgAQP1 in the Malaria Vector Anopheles gambiae. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0075888"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Piermarini, P.M. (2016). Renal Excretory Processes in Mosquitoes, Elsevier Ltd.. [1st ed.].","DOI":"10.1016\/bs.aiip.2016.04.003"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1111\/j.1365-2915.1987.tb00351.x","article-title":"Proteolytic Activity in the Ectoperitrophic Fluid of Blood-fed Culex nigripalpus","volume":"1","author":"Romoser","year":"1987","journal-title":"Med. Vet. Entomol."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Clements, A.N. (1992). The Biology of Mosquitoes: Development, Nutrition and Reproduction, Chapman & Hall.","DOI":"10.1079\/9780851993744.0000"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"e01962-21","DOI":"10.1128\/jvi.01962-21","article-title":"RACK1 Associates with RNA-Binding Proteins Vigilin and SERBP1 to Facilitate Dengue Virus Replication","volume":"96","author":"Brugier","year":"2022","journal-title":"J. Virol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1038\/s41564-019-0619-y","article-title":"Successive Blood Meals Enhance Virus Dissemination within Mosquitoes and Increase Transmission Potential","volume":"5","author":"Armstrong","year":"2020","journal-title":"Nat. Microbiol."}],"container-title":["Insects"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4450\/14\/3\/274\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:51:24Z","timestamp":1760122284000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4450\/14\/3\/274"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,9]]},"references-count":66,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2023,3]]}},"alternative-id":["insects14030274"],"URL":"https:\/\/doi.org\/10.3390\/insects14030274","relation":{},"ISSN":["2075-4450"],"issn-type":[{"value":"2075-4450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,9]]}}}