{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T03:32:52Z","timestamp":1773027172865,"version":"3.50.1"},"reference-count":30,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,4,23]],"date-time":"2023-04-23T00:00:00Z","timestamp":1682208000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170526"],"award-info":[{"award-number":["32170526"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170505"],"award-info":[{"award-number":["32170505"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32072419"],"award-info":[{"award-number":["32072419"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32070502"],"award-info":[{"award-number":["32070502"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31730074"],"award-info":[{"award-number":["31730074"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["20210302123473"],"award-info":[{"award-number":["20210302123473"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Natural Science Foundation of Shanxi Province","award":["32170526"],"award-info":[{"award-number":["32170526"]}]},{"name":"Natural Science Foundation of Shanxi Province","award":["32170505"],"award-info":[{"award-number":["32170505"]}]},{"name":"Natural Science Foundation of Shanxi Province","award":["32072419"],"award-info":[{"award-number":["32072419"]}]},{"name":"Natural Science Foundation of Shanxi Province","award":["32070502"],"award-info":[{"award-number":["32070502"]}]},{"name":"Natural Science Foundation of Shanxi Province","award":["31730074"],"award-info":[{"award-number":["31730074"]}]},{"name":"Natural Science Foundation of Shanxi Province","award":["20210302123473"],"award-info":[{"award-number":["20210302123473"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Group I chitin deacetylases (CDAs), CDA1 and CDA2, play an essential role in cuticle formation and molting in the process of insect wing development. A recent report showed that trachea are able to take up a secreted CDA1 (serpentine, serp) produced in the fat body to support normal tracheal development in the fruit fly Drosophila melanogaster. However, whether CDAs in wing tissue were produced locally or derived from the fat body remains an open question. To address this question, we applied tissue-specific RNAi against DmCDA1 (serpentine, serp) and DmCDA2 (vermiform, verm) in the fat body or the wing and analyzed the resulting phenotypes. We found that repression of serp and verm in the fat body had no effect on wing morphogenesis. RT-qPCR showed that RNAi against serp or verm in the fat body autonomously reduced their expression levels of serp or verm in the fat body but had no non-autonomous effect on the expression in wings. Furthermore, we showed that inhibition of serp or verm in the developing wing caused wing morphology and permeability deficiency. Taken together, the production of Serp and Verm in the wing was autonomous and independent of the fat body.<\/jats:p>","DOI":"10.3390\/insects14050406","type":"journal-article","created":{"date-parts":[[2023,4,24]],"date-time":"2023-04-24T02:31:05Z","timestamp":1682303465000},"page":"406","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Serpentine and Vermiform Are Produced Autonomously to Fulfill Their Function in Drosophila Wings"],"prefix":"10.3390","volume":"14","author":[{"given":"Xubo","family":"Zhang","sequence":"first","affiliation":[{"name":"Shanxi Key Laboratory of Nucleic Acid Biopesticides, Research Institute of Applied Biology, Shanxi University, Taiyuan 030006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanan","family":"Ji","sequence":"additional","affiliation":[{"name":"Shanxi Key Laboratory of Nucleic Acid Biopesticides, Research Institute of Applied Biology, Shanxi University, Taiyuan 030006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"Moussian","sequence":"additional","affiliation":[{"name":"INRAE, CNRS, Institut Sophia Agrobiotech, Sophia Antipolis, Universit\u00e9 C\u00f4te d\u2032Azur, 06108 Nice, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shumin","family":"Yang","sequence":"additional","affiliation":[{"name":"Shanxi Key Laboratory of Nucleic Acid Biopesticides, Research Institute of Applied Biology, Shanxi University, Taiyuan 030006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianzhen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanxi Key Laboratory of Nucleic Acid Biopesticides, Research Institute of Applied Biology, Shanxi University, Taiyuan 030006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3894-837X","authenticated-orcid":false,"given":"Tingting","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanxi Key Laboratory of Nucleic Acid Biopesticides, Research Institute of Applied Biology, Shanxi University, Taiyuan 030006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanxi Key Laboratory of Nucleic Acid Biopesticides, Research Institute of Applied Biology, Shanxi University, Taiyuan 030006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,23]]},"reference":[{"key":"ref_1","first-page":"12606","article-title":"Structure and mechanical properties of beetle wings: A review","volume":"2","author":"Sun","year":"2019","journal-title":"R. Soc. Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.femsle.2005.04.031","article-title":"The putative chitin deacetylase of Encephalitozoon cuniculi: A surface protein implicated in microsporidian spore-wall formation","volume":"247","author":"Brosson","year":"2005","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2564","DOI":"10.1073\/pnas.90.7.2564","article-title":"Bioconversion of chitin to chitosan: Purification and characterization of chitin deacetylase from Mucor rouxii","volume":"90","author":"Kafetzopoulos","year":"1993","journal-title":"PNAS"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"26286","DOI":"10.1074\/jbc.270.44.26286","article-title":"Purification and characterization of chitin deacetylase from Colletotrichum lindemuthianum","volume":"270","author":"Tsigos","year":"1995","journal-title":"J. Biol. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1890","DOI":"10.1111\/nph.15510","article-title":"Comparative characterization of putative chitin deacetylases from Phaeodactylum tricornutum and Thalassiosira pseudonana highlights the potential for distinct chitin-based metabolic processes in diatoms","volume":"221","author":"Shao","year":"2019","journal-title":"New Phytol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"110325","DOI":"10.1016\/j.cbpb.2019.110325","article-title":"Chitin deacetylase 1 and 2 are indispensable for larval\u2013pupal and pupal\u2013adult molts in Heortia vitessoides (Lepidoptera: Crambidae)","volume":"237","author":"Wang","year":"2019","journal-title":"Comp. Biochem. Physiol. Part B Biochem. Mol. Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1016\/j.ibmb.2007.12.002","article-title":"Domain organization and phylogenetic analysis of proteins from the chitin deacetylase gene family of Tribolium castaneum and three other species of insects","volume":"38","author":"Dixit","year":"2008","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_8","first-page":"44","article-title":"Extracellular Composite Matrices in Arthropods","volume":"15","author":"Cohen","year":"2016","journal-title":"Camb. Int. Law J."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1007\/s002530100741","article-title":"Shrimp chitin as substrate for fungal chitin deacetylase","volume":"57","author":"Win","year":"2001","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1016\/j.fsi.2016.06.025","article-title":"Molecular cloning and expression of chitin deacetylase 1 gene from the gills of Penaeus monodon (black tiger shrimp)","volume":"55","author":"Sarmiento","year":"2016","journal-title":"Fish Shellfish. Immunol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Heustis, R.J., Ng, H.K., Brand, K.J., Rogers, M.C., Le, L.T., Specht, C.A., and Fuhrman, J.A. (2012). Pharyngeal polysaccharide deacetylases affect development in the nematode C. elegans and deacetylate chitin in vitro. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0040426"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"24352","DOI":"10.1074\/jbc.M116.720581","article-title":"Helicoidal Organization of Chitin in the Cuticle of the Migratory Locust Requires the Function of the Chitin Deacetylase2 Enzyme (LmCDA2)","volume":"291","author":"Yu","year":"2016","journal-title":"J. Biol. Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1306","DOI":"10.1080\/09168451.2019.1591264","article-title":"Comparative characterization of putative chitin deacetylases from Tetranychus cinnabarinus","volume":"83","author":"Xiang","year":"2019","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Zhang, Z., Yan, J., Liu, Q., Zhang, Y., Gong, J., and Hou, Y. (2019). Genome-Wide Analysis and Hormone Regulation of Chitin Deacetylases in Silkworm. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20071679"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1016\/j.ibmb.2005.06.003","article-title":"A novel chitin-binding protein identified from the peritrophic membrane of the cabbage looper, Trichoplusia ni","volume":"35","author":"Guo","year":"2005","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1111\/imb.12554","article-title":"LmCDA1 organizes the cuticle by chitin deacetylation in Locusta migratoria","volume":"28","author":"Yu","year":"2019","journal-title":"Insect Mol. Biol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.ibmb.2019.05.008","article-title":"The putative chitin deacetylases Serpentine and Vermiform have non-redundant functions during Drosophila wing development","volume":"110","author":"Zhang","year":"2019","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.cub.2005.11.072","article-title":"Serpentine and vermiform encode matrix proteins with chitin binding and deacetylation domains that limit tracheal tube length in Drosophila","volume":"16","author":"Luschnig","year":"2006","journal-title":"Curr. Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.cub.2005.11.074","article-title":"Septate-junction-dependent luminal deposition of chitin deacetylases restricts tube elongation in the Drosophila trachea","volume":"16","author":"Wang","year":"2006","journal-title":"Curr. Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.jinsphys.2019.01.005","article-title":"Biochemical characterization of three midgut chitin deacetylases of the Lepidopteran insect Bombyx mori","volume":"113","author":"Liu","year":"2019","journal-title":"J. Insect Physiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.ibmb.2009.02.002","article-title":"Analysis of functions of the chitin deacetylase gene family in Tribolium castaneum","volume":"39","author":"Arakane","year":"2009","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"4104","DOI":"10.1242\/dev.109975","article-title":"A fat body-derived apical extracellular matrix enzyme is transported to the tracheal lumen and is required for tube morphogenesis in Drosophila","volume":"141","author":"Dong","year":"2014","journal-title":"Development"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Chapman, R.F., and Chapman, R.F. (1998). The Insects: Structure and function, Cambridge university press.","DOI":"10.1017\/CBO9780511818202"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"20152994","DOI":"10.1098\/rspb.2015.2994","article-title":"Regionalization of surface lipids in insects","volume":"283","author":"Wang","year":"2016","journal-title":"Proc. R. Soc. B Biol. Sci."},{"key":"ref_25","first-page":"1396","article-title":"Double cuticle barrier in two global pests, the whitefly Trialeurodes vaporariorum and the bedbug Cimex lectularius","volume":"220","author":"Wang","year":"2017","journal-title":"J. Exp. Biol."},{"key":"ref_26","first-page":"856369","article-title":"Fluorescent microscopy-based detection of chitin in intact Drosophila melanogaster","volume":"694","author":"Moussian","year":"2022","journal-title":"Front. Physiol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Otaki, J.M., and Nakazato, Y. (2022). Butterfly Wing Color Pattern Modification Inducers May Act on Chitin in the Apical Extracellular Site: Implications in Morphogenic Signals for Color Pattern Determination. Biology, 11.","DOI":"10.3390\/biology11111620"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1111\/j.1365-2583.2008.00829.x","article-title":"A chitin deacetylase and putative insect intestinal lipases are components of the Mamestra configurata (Lepidoptera: Noctuidae) peritrophic matrix","volume":"17","author":"Toprak","year":"2008","journal-title":"Insect Mol. Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"6985","DOI":"10.1074\/jbc.RA117.001454","article-title":"Group I chitin deacetylases are essential for higher order organization of chitin fibers in beetle cuticle","volume":"293","author":"Noh","year":"2018","journal-title":"J. Biol. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.lfs.2016.10.019","article-title":"Fat body remodeling and homeostasis control in Drosophila","volume":"167","author":"Zheng","year":"2016","journal-title":"Life Sci."}],"container-title":["Insects"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4450\/14\/5\/406\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:21:58Z","timestamp":1760124118000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4450\/14\/5\/406"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,23]]},"references-count":30,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2023,5]]}},"alternative-id":["insects14050406"],"URL":"https:\/\/doi.org\/10.3390\/insects14050406","relation":{},"ISSN":["2075-4450"],"issn-type":[{"value":"2075-4450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,23]]}}}