{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:06:23Z","timestamp":1760148383280,"version":"build-2065373602"},"reference-count":35,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,4,27]],"date-time":"2023-04-27T00:00:00Z","timestamp":1682553600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institute of Food and Agriculture","award":["MAS00527\/S51076"],"award-info":[{"award-number":["MAS00527\/S51076"]}]},{"name":"U.S. Department of Agriculture","award":["MAS00527\/S51076"],"award-info":[{"award-number":["MAS00527\/S51076"]}]},{"name":"the Massachusetts Agricultural Experiment Station","award":["MAS00527\/S51076"],"award-info":[{"award-number":["MAS00527\/S51076"]}]},{"name":"Stockbridge School of Agriculture at the Univ. of Massachusetts, Amherst","award":["MAS00527\/S51076"],"award-info":[{"award-number":["MAS00527\/S51076"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>Infection with salivary gland hypertrophy virus (MdSGHV) of Musca domestica prevents female flies from accepting copulation attempts by healthy or virus-infected males. This study focused on supplemental hormonal rescue therapy for mating behavior in virus-infected female house flies. The inhibitory effect of the virus on mating behavior in females injected with MdSGHV was reversed by hormonal therapy in the form of octopamine injections, topical application of methoprene, or both therapies combined along with 20-hydroxyecdysone. Infected females whose mating responsiveness had been restored continued to have other viral pathologies associated with infection such as hypertrophy of the salivary glands and a lack of ovarian development.<\/jats:p>","DOI":"10.3390\/insects14050416","type":"journal-article","created":{"date-parts":[[2023,4,27]],"date-time":"2023-04-27T02:18:34Z","timestamp":1682561914000},"page":"416","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rescuing the Inhibitory Effect of the Salivary Gland Hypertrophy Virus of Musca domestica on Mating Behavior"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-9205-7839","authenticated-orcid":false,"given":"Marissa","family":"Gallagher","sequence":"first","affiliation":[{"name":"Neuroscience Department, University of Massachusetts, Amherst, MA 01003, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9582-6432","authenticated-orcid":false,"given":"Arianna","family":"Ramirez","sequence":"additional","affiliation":[{"name":"Biology Department, University of Massachusetts, Amherst, MA 01003, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6187-8162","authenticated-orcid":false,"given":"Christopher J.","family":"Geden","sequence":"additional","affiliation":[{"name":"Center for Medical, Agricultural and Veterinary Entomology, USDA, Agricultural Research Service, Gainesville, FL 32608, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"John G.","family":"Stoffolano","sequence":"additional","affiliation":[{"name":"Stockbridge School of Agriculture, University of Massachusetts, Amherst, MA 01003, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"6776","DOI":"10.1111\/mec.16179","article-title":"Virus infection modulates male sexual behaviour in Caenorhabditis elegans","volume":"30","author":"Liu","year":"2021","journal-title":"Mol. Ecol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1673\/031.005.0601","article-title":"Infection with the insect virus Hz-2v alters mating behavior and pheromone production in female Helicoverpa zea moths","volume":"5","author":"Burand","year":"2005","journal-title":"J. Insect Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"287","DOI":"10.3390\/insects4030287","article-title":"Virology, epidemiology and pathology of Glossina hytrosavirus, and its control prospects in laboratory colonies of the tsetse fly, Glossina pallidipes (Diptera; Glossinidae)","volume":"4","author":"Kariithi","year":"2013","journal-title":"Insects"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.cois.2017.05.009","article-title":"Hytrosaviruses: Current status and perspective","volume":"22","author":"Kariithi","year":"2017","journal-title":"Curr. Opin. Ins. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1093\/jmedent\/45.1.42","article-title":"Seasonal prevalence and transmission of salivary gland hypertrophy virus of house flies (Diptera: Muscidae)","volume":"45","author":"Geden","year":"2008","journal-title":"J. Med. Entomol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"337","DOI":"10.3920\/978-90-8686-863-6_12","article-title":"Biological control of livestock pests: Entomopathogens","volume":"Volume 5","author":"Garros","year":"2018","journal-title":"Ecology and Control of Vector-Borne Diseases"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Mutika, G.N., Marin, C., Parker, A.G., Vreysen, M.J.B., and Boucias, D.G. (2012). Abd-Alla AMM: Impact of salivary gland hypertrophy virus infection on the mating success of male Glossina pallidipes: Consequences for the sterile insect technique. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0042188"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1111\/j.1365-2915.1993.tb00688.x","article-title":"Characterization and description of a virus causing salivary gland hyperplasia in the housefly, Musca domestica","volume":"7","author":"Coler","year":"1993","journal-title":"Med. Vet. Entomol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"6811","DOI":"10.1128\/AEM.02694-06","article-title":"Effects of salivary gland hypertrophy virus on the reproductive behavior of the housefly, Musca domestica","volume":"73","author":"Lietze","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"583","DOI":"10.3389\/fmicb.2017.00583","article-title":"Responses of the housefly, Musca domestica, to the hytrosavirus replication: Impacts on host\u2019s vitellogenesis and immunity","volume":"8","author":"Kariithi","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1146\/annurev.en.41.010196.002353","article-title":"Sexual receptivity in insects","volume":"41","author":"Ringo","year":"1996","journal-title":"Annu. Rev. Entomol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3708","DOI":"10.1073\/pnas.97.7.3708","article-title":"Mating experience and juvenile hormone enhance sexual signaling and mating in male Caribbean fruit flies","volume":"97","author":"Teal","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1093\/aesa\/66.6.1249","article-title":"The courtship of Musca species found in North America, 1. The housefly, Musca domestica","volume":"66","author":"Tobin","year":"1973","journal-title":"Ann. Entomol. Soc. Am."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1016\/S0022-1910(99)00047-5","article-title":"Regulation of mating behavior by nutrition and the corpus allatum in both male and female Phormia regina (Meigen)","volume":"45","author":"Yin","year":"1999","journal-title":"J. Insect Physiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1007\/BF02765373","article-title":"A pharmacological and endocrinological study of female insemination in Phormia regina (Diptera: Calliphoridae)","volume":"10","author":"Evans","year":"1997","journal-title":"J. Insect Behav."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/S0065-2806(08)60143-5","article-title":"Biogenic amines in the insect nervous system","volume":"15","author":"Evans","year":"1980","journal-title":"Adv. Insect Physiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1093\/jisesa\/iey040","article-title":"Effect of diet on adult house fly (Diptera: Muscidae) injected with the salivary gland hypertrophy virus (MdSGHV)","volume":"18","author":"Schaler","year":"2018","journal-title":"J. Insect Sci."},{"key":"ref_18","unstructured":"Sokal, R.R., and Rohlf, F.J. (1980). Biometry, W. H. Freeman."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1038\/2111321b0","article-title":"Corpus allatum and sexual receptivity in female Drosophila melanogaster","volume":"211","author":"Manning","year":"1966","journal-title":"Nature"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/0022-1910(69)90269-8","article-title":"Relationship of age, ovarian development, and the corpus allatum to mating in the housefly, Musca domestica","volume":"15","author":"Adams","year":"1969","journal-title":"J. Insect Physiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1146\/annurev.en.18.010173.002305","article-title":"Neuro-hormonal control of sexual behavior in insects","volume":"18","author":"Barth","year":"1973","journal-title":"Ann. Rev. Entomol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/B978-012532104-4\/50038-X","article-title":"Hormonal regulation of sexual behavior in insects","volume":"3","author":"Ringo","year":"2002","journal-title":"Horm. Brain Behav."},{"key":"ref_23","first-page":"353","article-title":"Hormonal control of diapause","volume":"Volume 8","author":"Kerkut","year":"1985","journal-title":"Comprehensive Insect Physiology, Biochemistry and Pharmacology"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1139\/z74-130","article-title":"Influence of diapause and diet on the development of the gonads and accessory reproductive glands of the black blowfly, Phormia regina (Meigen)","volume":"52","author":"Stoffolano","year":"1974","journal-title":"Canandian J. Zool."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"639","DOI":"10.2108\/zsj.16.639","article-title":"Role of the corpus allatum in the control of reproductive diapause in the male blow fly, Protophormia terraenovae","volume":"16","author":"Tanigawa","year":"1999","journal-title":"Zool. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/0022-1910(89)90053-X","article-title":"Precocene II treatment inhibits terminal oocyte development but not vitellogenin synthesis in the black blowfly Phormia regina","volume":"35","author":"Yin","year":"1989","journal-title":"J. Insect Physiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/j.jinsphys.2005.01.007","article-title":"Juvenile hormone, 20-hydroxyecdysone and dopamine interaction in Drosophila virilis reproduction under normal and nutritional stress conditions","volume":"51","author":"Gruntenko","year":"2005","journal-title":"J. Insect Physiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1002\/arch.20187","article-title":"Effects of octopamine on reproduction, juvenile hormone metabolism, dopamine, and 20-hydroxyecdysone contents in Drosophila","volume":"65","author":"Gruntenko","year":"2007","journal-title":"Arch. Insect Biochem. Physiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1134\/S0012496606020190","article-title":"Dopamine affects the level of 20-hydroxyecdysone in Drosophila virilis females","volume":"407","author":"Rauschenbach","year":"2006","journal-title":"Dokl. Biol. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"902","DOI":"10.1016\/j.jinsphys.2008.04.004","article-title":"Interplay of JH, 20E and biogenic amines under normal and stress conditions and its effect on reproduction","volume":"54","author":"Gruntenko","year":"2008","journal-title":"J. Insect Physiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.jinsphys.2006.11.009","article-title":"Comparing injection, feeding and topical application methods for treatment of honeybees with octopamine","volume":"53","author":"Barron","year":"2007","journal-title":"J. Insect Physiol."},{"key":"ref_32","first-page":"3178","article-title":"Precocene-I inhibits juvenile hormone biosynthesis, ovarian activation, aggression and alters sterility signal production in bumble bee (Bombus terrestris) workers","volume":"217","author":"Amsalem","year":"2014","journal-title":"J. Exp. Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"994","DOI":"10.1128\/AEM.02424-09","article-title":"Musca domestica salivary gland hypertrophy virus, a globally distributed insect virus that infects and sterilizes female houseflies","volume":"76","author":"Prompiboon","year":"2010","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.jip.2014.10.004","article-title":"Enhancement of the Musca domestica hytrosavirus infection with orally delivered reducing agents","volume":"124","author":"Boucias","year":"2015","journal-title":"J. Invert. Pathol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1111\/j.1948-7134.2011.00163.x","article-title":"Salivary gland hypertrophy virus of house flies in Denmark: Prevalence, host range, and comparison with a Florida isolate","volume":"36","author":"Geden","year":"2011","journal-title":"J. Vector Ecol."}],"container-title":["Insects"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2075-4450\/14\/5\/416\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:24:08Z","timestamp":1760124248000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2075-4450\/14\/5\/416"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,27]]},"references-count":35,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2023,5]]}},"alternative-id":["insects14050416"],"URL":"https:\/\/doi.org\/10.3390\/insects14050416","relation":{},"ISSN":["2075-4450"],"issn-type":[{"type":"electronic","value":"2075-4450"}],"subject":[],"published":{"date-parts":[[2023,4,27]]}}}