{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T03:26:18Z","timestamp":1783826778546,"version":"3.55.0"},"reference-count":28,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T00:00:00Z","timestamp":1690156800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Insects"],"abstract":"<jats:p>The brown dog tick, Rhipicephalus sanguineus s.l., is considered the most widely distributed three-host tick in the world and has medical and veterinary importance; the control of infestation is carried out with acaricides, towards which it can develop resistance. This study aimed to determine the discriminant dose (d.d) of amitraz to identify resistance in R. sanguineus s.l. larvae natives from Mexico and to evaluate its application in field-collected ticks. Engorged ticks were collected from naturally infested dogs residing in rural communities and were incubated for 25 days, and their progeny was used in a larval immersion test (LIT) to be exposed to the d.d. determined in Rhipicephalus microplus, and those that were susceptible were analyzed using the LIT in six concentrations. Mortality was analyzed through probit methodology to calculate the lethal concentration (LC) 50 and 99. The d.d. was determined as a consensus value by multiplying the LC99 \u00d7 2, and then, we proceeded to evaluate it in in-field samples by using the LIT technique. The d.d. calculated was 4 ppm. The in-field evaluation found 64% of the resistant samples to amitraz with mortality percentages between 98.3% and 0.35%. This dose can be used to rapidly and inexpensively identify resistant populations in samples collected in the field.<\/jats:p>","DOI":"10.3390\/insects14070662","type":"journal-article","created":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T01:32:10Z","timestamp":1690248730000},"page":"662","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Determination of a Discriminant Dose to Identify Resistance to Amitraz in Rhipicephalus sanguineus s.l. (Acari: Ixodidae) from Mexico"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0422-3482","authenticated-orcid":false,"given":"Francisco","family":"Mart\u00ednez-Iba\u00f1ez","sequence":"first","affiliation":[{"name":"Tecnol\u00f3gico Nacional de M\u00e9xico, Instituto Tecnol\u00f3gico El Llano Aguascalientes, Km. 18 Carretera Aguascalientes-San Luis Potos\u00ed, El Llano 20330, Aguascalientes, Mexico"},{"name":"Centro Nacional de Servicios en Constataci\u00f3n en Salud Animal, Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria Carretera Cuernavaca-Cuautla 8534, Col. Progreso, Jiutepec 62550, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0970-6811","authenticated-orcid":false,"given":"Carlos","family":"Cruz-V\u00e1zquez","sequence":"additional","affiliation":[{"name":"Tecnol\u00f3gico Nacional de M\u00e9xico, Instituto Tecnol\u00f3gico El Llano Aguascalientes, Km. 18 Carretera Aguascalientes-San Luis Potos\u00ed, El Llano 20330, Aguascalientes, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Osorio-Miranda","sequence":"additional","affiliation":[{"name":"Centro Nacional de Servicios en Constataci\u00f3n en Salud Animal, Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria Carretera Cuernavaca-Cuautla 8534, Col. Progreso, Jiutepec 62550, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Vitela-Mendoza","sequence":"additional","affiliation":[{"name":"Tecnol\u00f3gico Nacional de M\u00e9xico, Instituto Tecnol\u00f3gico El Llano Aguascalientes, Km. 18 Carretera Aguascalientes-San Luis Potos\u00ed, El Llano 20330, Aguascalientes, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leticia","family":"Medina-Esparza","sequence":"additional","affiliation":[{"name":"Tecnol\u00f3gico Nacional de M\u00e9xico, Instituto Tecnol\u00f3gico El Llano Aguascalientes, Km. 18 Carretera Aguascalientes-San Luis Potos\u00ed, El Llano 20330, Aguascalientes, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5474-9816","authenticated-orcid":false,"given":"Rodolfo","family":"Lagunes-Quintanilla","sequence":"additional","affiliation":[{"name":"Centro Nacional de Investigaci\u00f3n Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agr\u00edcolas y Pecuarias Carretera Cuernavaca-Cuautla 8534, Col. Progreso, Jiutepec 62550, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3783-3255","authenticated-orcid":false,"given":"Arturo","family":"Ch\u00e1vez-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Tecnol\u00f3gico Nacional de M\u00e9xico, Instituto Tecnol\u00f3gico de Tlajomulco, Km. 10 Carretera Tlajomulco, Cto. Metropolitano Sur, Tlajomulco de Zu\u00f1iga 45640, Jalisco, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.vetpar.2007.12.030","article-title":"The brown dog tick, Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae): From taxonomy to control","volume":"152","year":"2008","journal-title":"Vet. 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